Neurotoxic action of permethrin in Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) female ticks. Morphological and cytochemical evaluation of the central nervous system

Studies on the molecular bases of the neurotoxic action of acaricides are found in the literature; but there are no studies of this action on the nervous system of ticks at the cellular level. The present study describes the morphological and cytochemical changes in the synganglion of Rhipicephalus sanguineus semi-engorged females exposed to different concentrations of permethrin, a pyrethroid with recognized neurotoxic action. Permethrin induced the degeneration of the synganglion through a process of apoptosis involving autophagy, characterized by the condensation and margination of the chromatin, formation of blebs in the nuclear envelope and fragmentation of the nucleus, loss of shape of neural cells and integrity of cellular membrane, cytoplasmic shrinkage, and lower levels of acid phosphatase in the nervous tissue as the concentration of permethrin increased. This study provided further evidence of the neurotoxic action of permethrin, which impairs the metabolism of R. sanguineus nervous systems, and consequently the physiology of other systems, dependent on the neural control. These results provide cytochemical and histological confirmation of the neurotoxic action of permethrin, previously inferred from molecular and tick behavioral evidence. © 2013 Elsevier B.V.

Action of andiroba oil and permethrin on the central nervous and reproductive systems of Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) ticks females. A confocal study

Research for acaricides with lower toxicity and impact on the environment has been intensified. An alternative would be the use of natural compounds or of synthetic products in lower concentrations than the ones sold commercially. Thus, this study describes the action of andiroba seed oil on the nuclei of the ovary and synganglion cells of Rhipicephalus sanguineus, and presents an analysis of the nuclear morphology of the nervous system cells of this tick species when exposed to permethrin. The results obtained showed that, although no changes have been observed in the genetic material of the ovary cells exposed to the andiroba oil, this compound, as well as permethrin, has neurotoxic action on the females of this species. The damages caused to the physiology of the synganglion, due to the loss of integrity of the genetic material, would result in the impairment of the metabolism of other systems of R. sanguineus ticks. © 2013 Elsevier Ltd.

Crescimento, produtividade, acúmulo e exportação de nutrientes em cultivares de batata (Solanum tuberosum L.)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Inibidores de etileno na pós-colheita de Lisianthus

Pós-graduação em Agronomia (Horticultura) - FCA

Controle da mancha angular do feijoeiro com fungicidas e efeito na produção de plantas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

The economics of climate change

This paper focuses on several key economic issues relevant to climate change. Given the fact that changes in climate are projections using the best available data, it examines the economic principles of uncertainty and the precautionary approach, and then continues to address the key drivers of climate change. Climate change is expected to result in negative impacts and, in this regard, the consequences of anticipated rising temperatures and sea levels as well as changes in precipitation that may result in flooding and/or drought are addressed. However, the situation is not all negative and, as such, the opportunities that are likely to arise through adaptation and mitigation are discussed especially with respect to the Caribbean. In this regard and recognizing that it would be useful to Caribbean policymakers to utilize these opportunities and to address the negative impacts, the Economic Commission for Latin America and the Caribbean (ECLAC), through its subregional headquarters for the Caribbean, is spearheading a review of the economics of climate change in the Caribbean with a view to providing quantitative information to stakeholders in dealing with climate change. The paper makes recommendations for the Caribbean to address climate change at the national level. These include macroplanning; promotion of energy efficiency; creation of incentives to obtain support for implementation of alternative energy technologies; maintaining the will among stakeholders on a sustained basis for addressing climate change; and the conduct of relevant research into varieties of plants and animals that could adapt to changing climatic conditions. Finally, it must be recognized that to successfully combat climate change, the threats and opportunities must be properly assessed as part of an ongoing region-wide system of risk management, which should not be incidental, but integral, to national and regional planning and forecasting for the future.

Modos de parcelamento da aplicação de regulador de crescimento em função de espaçamentos e densidades populacionais em algodoeiro

Pós-graduação em Agronomia - FEIS

Caracterização de Puccinia psidii, identificação de mirtáceas diferenciadoras de raças fisiológicas e estudos anatômicos do limbo foliar relacionados à resistência

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Sombreamento e aplicação de nitrogênio em plantas jovens de Jatropha curcas L

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação de cultivares de milho em função de populações de plantas e épocas de semeadura

Pós-graduação em Agronomia (Agricultura) - FCA

Influência do frio e do défice hídrico nas características fisiológicas e bioquímicas em videiras (Vitis vinífera L.) cv. Thompson seedless

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ethnoveterinary knowledge of the inhabitants of Marajó Island, Eastern Amazonia, Brazil

Em várias partes do mundo existem relatos etnoveterinários sobre a utilização de plantas em protocolos terapêuticos, entretanto não existem informações disponíveis sobre a etnoveterinária praticada na Amazônia brasileira. Desta forma, objetivou-se documentar o conhecimento etnoveterinário de habitantes da Ilha do Marajó, Amazônia Oriental. Foram realizadas 50 entrevistas individuais com aplicação de questionários semi-estruturados que foram analisados quantitativamente através de estatística descritiva utilizando freqüência de distribuição. O valor de uso foi calculado para determinar as espécies mais importantes. Amostras de plantas com relatos de uso medicinal foram coletadas e identificadas botanicamente. Cinqüenta plantas, distribuídas em 48 gêneros e 34 famílias, foram indicadas para 21 diferentes usos medicinais. A família Asteraceae foi a que teve maior número de espécies citadas e Carapa guianensis Aubl, Crescentia cujete L., Copaifera martii Hayne, Caesalpinia ferrea Mart., Chenopodium ambrosioides L., Jatropha curcas L. e Momordica charantia L. foram as espécies com maiores valor de uso. As partes das plantas mais utilizadas para preparo dos medicamentos etnoveterinários foram folhas (56%), cascas (18%), raizes (14%), sementes (14%) e frutos (8%). Quanto à forma de uso o chá foi citado por 56% dos entrevistados e a maioria das preparações (90,9%) utiliza uma só planta. Além das plantas medicinais, os entrevistados relataram o uso de produtos de origem animal e mineral. Esse trabalho contribui para realização de um inventário das plantas utilizadas na etnoveterinária marajoara que pode servir de base de dados para futuros estudos de validação científica.

Sequence characterization of coding regions of the myostatin gene (GDF8) from Brazilian Murrah buffaloes (Bubalus bubalis) and comparison with the Bos taurus sequence

Within about 30 years the Brazilian buffalo (Bubalus bubalis) herd will reach approximately 50 million head as a result of the great adaptive capacity of these animals to tropical climates, together with the good productive and reproductive potential which make these animals an important animal protein source for poor and developing countries. The myostatin gene (GDF8) is important in the physiology of stock animals because its product produces a direct effect on muscle development and consequently also on meat production. The myostatin sequence is known in several mammalian species and shows a high degree of amino acid sequence conservation, although the presence of non-silent and silent changes in the coding sequences and several alterations in the introns and untranslated regions have been identified. The objective of our work was to characterize the myostatin coding regions of B. bubalis (Murrah breed) and to compare them with the Bos taurus regions looking for variations in nucleotide and protein sequences. In this way, we were able to identify 12 variations at DNA level and five alterations on the presumed myostatin protein sequence as compared to non double-muscled bovine sequences.

Intraspecific variability of Holostylis reniformis: concentration of lignans, as determined by maceration and supercritical fluid extraction (SFE-CO2), as a function of plant provenance and plant parts

Maceration and supercritical fluid extraction were used to prepare extracts from parts of plants (Holostylis reniformis) collected in two different regions of Brazil. ¹H NMR, HPLC-DAD-ESI/MS, HPLC-DAD, GC-MS, and chemometric techniques were used to analyse lignans in the extracts and showed that yields of SFE-CO2 were less than or equal to those of hexane maceration extracts. These analyses, in conjunction with the concentrations of aliphatic hydrocarbons, fatty acids and their methyl and ethyl derivatives in the extracts, also allowed the chemical composition of parts and provenance of the plant to be differentiated.

Action of rain on the efficiency of herbicides applied post-emergence in the control of Senna obtusifolia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)