Purification and Characterization of an Ethanol-Tolerant β-Glucosidase from Sporidiobolus pararoseus and Its Potential for Hydrolysis of Wine Aroma Precursors

An extracellular ethanol-tolerant β-glucosidase from Sporidiobolus pararoseus was purified to homogeneity and characterized, and its potential use for the enhancement of wine aroma was investigated. The crude enzymatic extract was purified in four steps (concentration, dialysis, ultrafiltration, and chromatography) with a yield of around 40 % for total activity. The purified enzyme (designated Sp-βgl-P) showed a specific activity of approximately 20.0 U/mg, an estimated molecular mass of 63 kDa after sodium dodecyl sulfate polyacrylamide gel electrophoresis, and isoelectric point of 5.0 by isoelectric focusing. Sp-βgl-P has optimal activity at pH 4.0 and at 55 °C. It was stable in a broad pH range at low temperatures and it was tolerant to ethanol and glucose, indicating suitable properties for winemaking. The hydrolysis of glycosidic terpenes was analyzed by adding Sp-βgl-P directly to the wines. The released terpene compounds were evaluated by gas chromatography/mass spectrometry. The enzymatic treatment significantly increased the amount of free terpenes, suggesting that this enzyme could potentially be applicable in wine aroma improvement. © 2013 Springer Science+Business Media New York.

Antitumour and anti-inflammatory effects of palladium(II) complexes on Ehrlich tumour

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Time-course morphological and functional disorders of the kidney induced by long-term high-fat diet intake in female rats

BackgroundEvidence is emerging that highlights the far-reaching consequences of a high-fat diet (HFD) on kidney morphology and function disorders.MethodsThe present study was performed on 3-, 5-, 7- and 9-week-old HFD female rats compared with the appropriate gender and age-matched animals. We evaluated the kidney expression of angiotensin type II receptor and fibrotic and epithelial-to-mesenchymal transition (EMT) markers, by immunoblotting and immunohistochemical and histological techniques, in parallel with kidney function.ResultsIn the current study, the time-course HFD-treated group showed, by immunoblotting and immunohistochemical analysis, an early time-course increase in the expression of transforming growth factor β-1 (TGFβ-1) in the entire kidney of HFD-treated rats, compared with that observed in the control group. Simultaneously, the study shows a transient increase in the expression of ZEB2 in the HFD whole kidney accompanied by a fall in the E-cadherin expression and increased collagen and fibronectin deposition. A pronounced decrease in fractional urinary sodium excretion was also demonstrated in the long-term HFD-treated rats. The decreased FENa + was accompanied by a fall in FEPNa + and FEPP Na +, which occurred in association with significantly decreased CCr and, certainly on the sodium-filtered load. The reduction in the glomerular filtration rate (GFR) occurred in parallel to proteinuria and glomerular desmin overexpression.ConclusionsThe results of the current study suggest that podocyte injury in parallel with observed proteinuria and evidence of EMT transformation are associated with long-term loss of kidney function and renal sodium and water retention. © 2013 The Author. All rights reserved.

Alterações odontológicas em pacientes urêmicos em hemodiálise e suas possíveis correlações com neuropatias de nervos cranianos

Pós-graduação em Bases Gerais da Cirurgia - FMB

Atividade mutagênica e ativadora da resposta imune celular induzidas por Byrsonima crassa Niedenzu e Byrsonima intermedia A.Juss. (Malpighiaceae)

Pós-graduação em Biociências e Biotecnologia Aplicadas à Farmácia - FCFAR

Atividade física habitual, barreiras para prática de atividade física e indicadores de saúde de pacientes em hemodiálise

Pós-graduação em Ciências da Motricidade - IBRC

Estudo físico-químico de nanopartículas de DNA com derivado de quitosana contendo grupos fosforilcolina

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Susceptibilidade antifúngica, produção de biofilme e caracterização do gene ALS3 em isolados de Candida albicans e não-albicans do hospital das clínicas, UNESP, Botucatu

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da própolis in vivo sobre a expressão de receptores semelhantes a Toll (TLR-4 e TLR-2) e produção de citocinas por camundongos BALB/c

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito do suco de laranja e da glicosil hesperidina sobre o sistema imune inato de camundongos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Produtos nitrogenados na saliva de portadores de doença renal crônica em hemodiálise

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Digestibilidade das proteínas do soro do leite mediante ensaio in vitro

Pós-graduação em Alimentos e Nutrição - FCFAR

Avaliação de sessões de hemodiálise em cães portadores de injúria renal aguda assiciada à infecções

Pós-graduação em Medicina Veterinária - FMVZ

Preditores de incorreçao na prescrição de antimicrobianos no Hospital Estadual Bauru (Bauru, São Paulo)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação da citotoxicidade de cimentos endodônticos quanto a liberação de peróxido de hidrogênio e óxido nítrico em culturas de macrófagos peritoneais de camundongos

Pós-graduação em Odontologia - FOAR