A Laodicean; a story of today.

Paged continuously.

Five hundred books for the young,

Mode of access: Internet.

Contemporary American literature,

Mode of access: Internet.

The Child development associate program : prototype model : CDA training provider improvement system /

"Contract number 105-78-1016."

Chemical and biological quality of streams at the Indiana Dunes National Lakeshore, Indiana, 1978-80 /

Bibliography: p. 55-57.

Der praktische obstgärtner.

Bd. 3, Der Obstbaumschnitt, nach J.A. Hardy, Traite de la taille des arbres fruitiers. 1860.

Calendar to the Sessions records ... 1678 to

At head of title: County of Buckingham.

Lighthouses their history and romance /

Mode of access: Internet.

Lighthouses; their history and romance,

Mode of access: Internet.

When vicars meet: A narrow contact zone between morphologically cryptic phylogeographic lineages of the rainforest skink, Carlia rubrigularis

Phylogeographic analyses of the fauna of the Australian wet tropics rainforest have provided strong evidence for long-term isolation of populations among allopatric refugia, yet typically there is no corresponding divergence in morphology. This system provides an opportunity to examine the consequences of geographic isolation, independent of morphological divergence, and thus to assess the broader significance of historical subdivisions revealed through mitochondrial DNA phylogeography. We have located and characterized a zone of secondary contact between two long isolated (mtDNA divergence > 15%) lineages of the skink Carlia rubrigularis using one mitochondrial and eight nuclear (two intron, six microsatellite) markers. This revealed a remarkably narrow (width < 3 km) hybrid zone with substantial linkage disequilibrium and strong deficits of heterozygotes at two of three nuclear loci with diagnostic alleles. Cline centers were coincident across loci. Using a novel form of likelihood analysis, we were unable to distinguish between sigmoidal and stepped cline shapes except at one nuclear locus for which the latter was inferred. Given estimated dispersal rates of 90-133 m x gen(-1/2) and assuming equilibrium, the observed cline widths suggest effective selection against heterozygotes of at least 22-49% and possibly as high as 70%. These observations reveal substantial postmating isolation, although the absence of consistent deviations from Hardy-Weinberg equilibrium at diagnostic loci suggests that there is little accompanying premating isolation. The tight geographic correspondence between transitions in mtDNA and those for nuclear genes and corresponding evidence for selection against hybrids indicates that these morphologically cryptic phylogroups could be considered as incipient species. Nonetheless, we caution against the use of mtDNA phylogeography as a sole criterion for defining species boundaries.

Loss of PKR Activity in Chronic Lymphocytic Leukemia

There are a number of observations that suggest the dsRNA-activated protein kinase, PKR, may play an active role in formation and maintenance of leukemia, including nonrandom chromosomal deletions in acute leukemia as well as truncations and deletions of the PKR gene in some leukemia cell lines. However, there is little direct evidence from patient material that this is so. Here we show that full-length PKR is present but not active in 21 of 28 patient samples from B-cell chronic lymphocytic leukemia (B-CLL). PKR from these patients was unable to auto-activate or phosphorylate substrates but was able to bind dsRNA. Furthermore, the lack of PKR activation was not due to differing levels of the PKR activator, PACT nor of the PKR inhibitor, p58(IPK). We compared PKR status with clinical parameters and disease staging. No differences were found between the 2 groups in terms of staging (modified Rai or Binet), age, CD38 status, p53 status, 11q23 deletion status or CEP12 deletion status. However, there was a significant correlation between deletion in 13q14.3 and lack of PKR activity. We show that B-CLL cells appear to contain a soluble inhibitor of PKR, as lysates from cells lacking PKR activity were able to inhibit exogenous PKR in mixing experiments. Finally, we show suppression of PKR activity was still present following ultrafilitration through a 10,000 Da cutoff filter but was lost upon extraction with phenol/chloroform or by high salt washing. This data suggests loss of PKR activity may contribute to the formation and/or maintenance of CLL. (C) 2004 Wiley-Liss, Inc.

Ventilation rate and behavioural responses of two species of intertidal goby (Pisces : Gobiidae) at extremes of environmental temperature

We investigated the behavioural responses of two gobiid fish species to temperature to determine if differences in behaviour and ventilation rate might explain any apparent vertical zonation. A survey of the shore at Manly, Moreton Bay revealed Favonigobius exquisitus to dominate the lower shore and Pseudogobius sp. 4 the upper shore. These species were exposed to a range of temperatures (15-40 degreesC) in aquaria for up to 6 h. At 20 degreesC F. exquisitus exhibited a mean gill ventilation rate of 26 +/- 1.4 bpm (beats per minute) differing significantly from Pseudogobius, which ventilated at a fivefold greater rate of 143 +/- 6 bpm. The ventilation rate in F. exquisitus underwent a fivefold increase from normal local water temperature (20 degreesC) to high temperature (35 degreesC) conditions, whereas that of Pseudogobius did not even double, suggesting that Pseudogobius sp. is a better thermal regulator than F. exquisitus. While both species emerged from the water at high temperatures (>30 degreesC) the behaviours they exhibited while immersed at high temperature were quite different. F. exquisitus undertook vertical displacement movements we interpret as an avoidance response, whereas Pseudogobius sp. appeared to use a coping strategy involving movements that might renew the water mass adjacent to its body. The thermal tolerances and behaviours of F. exquisitus and Pseudogobius sp. are in broad agreement with their vertical distribution on the shore.