Infection of Anopheles darlingi fed on patients infected with Plasmodium vivax before and during treatment with chloroquine plus primaquine in Costa Marques, Rondônia, Brazil

Five patients with asexual and sexual parasites of Plasmodium vivax were treated orally with 600 mg chloroquine diphosphate (hour 0) followed with 300 mg at 8, 24 and 48 h later. Primaquine phospate, 15 mg, was administered concurrently at h 0 and 24 h intervals for 14 days. Anopheles darlingi were fed before the first dose (h-0.5) and 0.5, 1, 2, 4, 6, 8, 10, 12, 16, 20, 24, 36, 48, 60 and 72 h later. Mosquitoes were examined for oocysts on day 8 and for sporozoites on day 15 after infection. Four of the five patients studied were still infective to mosquitoes from 1-5 h after the first dose of chloroquine plus primaquine. One of these and one other patient, who vomited 15 min after the first dose, became inffective again at hours 10 and 12, respectively. Once produced, oocysts in mosquitoes fed on patients before, during and after chloroquine plus primaquine treatment appeared normal and produced sporozoite infected salivary glands. In view of these data , it is concluded that primaquine demonstrated rapid gametocytocidal activity and should be administred concurrently with chloroquine to reduce vivax malaria transmission.

Description of Raillietia flechtmani sp. n. (Acari; Gamasida)

Raillietia flechtmanni sp. n. is described from the ear canal of its type host the domestic buffalo. The new species parasitizes cattle as well in Brazil.

Preliminary investigations on transmission and life cycle of the ear mites of the genus Raillietia trouessart (Acari: Gamasida) parasites of cattle

The life cycle of ear mites of the genus Raillietia Trouessart consists of egg, larva, proto-and deutonymph and adult. The proto-and deutonymph are free living, non feeding instars. The teneral adult is the transfer stage. The minimum period required for completion of the life cycle is approximately eight days.

Escuela de Artes Dramáticas de la Universidad de Costa Rica, como formador de identidad teatral en Costa Rica

El presente trabajo de investigación tiene como objetivo plantear un análisis que estudie el desarrollo de la actividad teatral en Costa Rica a partir de la Escuela de Artes Dramáticas, como primera institución de enseñanza superior de teatro. La influencia que ha generado en el país, a través de un trabajo metódico y que forma un medio profesional que se fortalece con los años, convirtiéndose en institución de enseñanza teatral por excelencia y base del movimiento teatral por costarricense.

Visceral leishmaniasis in Teresina, state of Piauí, Brazil: preliminary observations on the detection and transmissibility of canine and sandfly infections

A Leishmania donovani-complex specific DNA probe was usedto confirm the widespread dissemination of amastigotes in apparently normal skinof dogs with canine visceral leishmaniasis. When Lutzomyia longipalpis were fed on abnormal skin of five naturally infected dogs 57 of 163 (35 per cent) fliesbecame infected: four of 65 flies (6 per cent) became infected when fed on apparently normal skin. The bite of a single sandfly that had fed seven days previouslyon a naturally infected dog transmitted the infection to a young dog from a non-endemic area. Within 22 days a lesion had developed at the site of the infectivebite (inner ear): 98 days after infection organisms had not disseminated throughout the skin, bone marrow, spleen or liver and the animal was still serologically negative by indirect immunofluorescence and dot-enzyme-linked immunosorbent assay. When fed Lu. longipalpis were captured from a kennel with a sick dog known to be infected, 33 out of 49 (67 per cent) of flies contained promastigotes. In contrast only two infections were detected among more than 200 sandflies captured in houses. These observations confirm the ease of transmissibility of L.chagasi from dog to sandfly to dog in Teresina. It is likely that canine VL is the major source of human VL by the transmission route dog-sandfly-human. the Lmet2 DNA probe was a useful epidemiological tool for detecting L. chagasi in sandflies.

¿Elecciones en 2009? Avances y obstáculos de la construcción de paz en Costa de Marfil

Tres meses de conflicto armado en Côte d'Ivoire en 2002 acabaron con la división del país en dos regiones, separadas por una línea de separación controlada por las francesas Forces Licorne. El proceso de paz se alargó en el tiempo y se caracterizó por una falta de confianza mutua y por la inmovilidad política. Estos hechos desembocaron en una situación de impasse y en la permanencia de Laurent Gbagbo en la presidencia del país. Además, los diferentes acuerdos políticos no ayudaron al proceso de construcción de paz, ya que no trataban algunos de los problemas principales del país, como la propiedad de las tierras y la identidad. Este documento de trabajo aspira, en primer lugar, a analizar los hechos principales y las causas que originaron el conflicto desde el golpe de estado de 2002. En segundo lugar, el documento analiza el proceso de paz y señala los elementos clave del Acuerdo de Paz de Ouagadougou (2007): la creación de una estructura de fuerzas armadas nueva y única, así como la identificación de la población y la convocatoria de elecciones. El objetivo principal es proporcionar una herramienta de trabajo al Institut Català Internacional per la Pau (ICIP) para enviar una futura comisión de observación electoral a este país africano.

Eleccions al 2009? Avenços i obstacles de la construcció de pau a Costa d'Ivori

Tres mesos de conflicte armat a Côte d’Ivoire durant el 2002 acabaren amb la divisió del país en dues regions, separades per una línia d’interposició controlada per les franceses Forces Licorne. El procés de pau es perllongà en el temps i es caracteritzà per una manca de confiança mútua i per una immobilitat política. Aquests fets desembocaren en una situació d’impasse i en la permanència de Laurent Gbagbo a la presidència del país. A més, els diferents acords polítics no ajudaren el procés de construcció de pau, ja que no encaraven alguns dels problemes principals del país, com ara la propietat de les terres i els assumptes d’identitat. Aquest document de treball aspira, en primer lloc, a analitzar els fets principals i les causes que originaren el conflicte des del cop d’estat del 2002. En segon lloc, el document analitza el procés de pau i assenyala els elements clau de l’Acord de Pau d’Ouagadougou (2007): la creació d’una estructura de forces armades nova i única, així com la identificació de la població i la realització d’un procés electoral. L’objectiu principal és proporcionar una eina de treball a l’Institut Català Internacional per la Pau (ICIP) per enviar una missió d’observació electoral a aquest país africà el novembre de 2009.

Analysis of the clonal diversity of Staphylococcus aureus methicillin-resistant strains isolated at João Pessoa, state of Paraíba, Brazil

To investigate the clonal diversity of Staphylococcus aureus strains isolated at João Pessoa, State of Paraíba, Brazil, digested genomic DNA were studied by pulsed-field gel electrophoresis (PFGE) in nine methicillin-resistant strains (MRSA) and three methicillin-sensitive strains (MSSA), selected among 67 isolates based on their antimicrobial susceptibility and epidemiology. The isolates were obtained between April and November 1992 from the Hospital of the Federal University of Paraíba, located in João Pessoa. Two MRSA isolates from the Oswaldo Cruz Hospital, São Paulo, Brazil, including an epidemic strain previously detected from different hospitals at the country were used as control. Five different patterns, were demonstrated by MRSA isolated in João Pessoa and these patterns were described in several epidemiologically unrelated hospitals in São Paulo. Our results suggest the interstate dissemination of a MRSA clone in João Pessoa which is similar to that described in other cities of Brazil.

Late Cretaceous and Paleogene Radiolaria from the Nicoya Peninsula, Costa Rica: a tectonostratigraphic application

Detailed field mapping and paleontological dating in the central and southeastern Nicoya Peninsula has revealed Late Cretaceous and Paleogene radiolarian-bearing siliceous mudstones. These rocks belong to two terranes (Matambfi and Manzanillo) that are partially contemporaneous with the Nicoya Complex, but are genetically different. While the Nicoya Complex is formed exclusively by intraplate igneous rocks with associated radiolarites, the studied sections include variable amounts of are-derived volcanic and terrigenous materials. These fore-arc terranes include mafic to intermediate volcaniclastics and associated pelagic and hemipelagic rocks rich in biogenic silica. Radiolarian preservation in these sediments is often enhanced by the presence of silica-saturated volcanic tuffs and debris. Seven out of 29 samples from different outcrops yielded relatively well-preserved radiolarian faunas. In total, 60 species belonging to 34 genera were present in these faunas, ranging in age from middle Turonian-Santonian to late Thanetian-Ypresian.

Condicions per a la consolidació de la pau a Costa d'Ivori. Relatoria del seminari Abidjan, Setembre 2010

Aquesta relatoria correspon al seminari internacional “Conditions pour la consolidation de la Paix en Côte d’Ivoire” coorganitzat per l’Institut Català Internacional per la Pau, el Centre de Recherche et Action pour la Paix (CERAP), l’Université de Bouaké, i la missió del PNUD a Costa d’Ivori, que es va realitzar a Abidjan entre el 27 i el 29 de setembre de 2010. La situació al país en el moment de la realització del seminari era difícil davant la celebració d’eleccions presidencials pocs mesos després. A la relatoria es recullen diferents aspectes sociopolítics que indicaven les greus fractures a les que s’enfrontava el país, així s’analitzen les qüestions relatives a la propietat de la terra, els problemes relacionats amb la nacionalitat i ciutadania ivoriana, les possibilitats de reorganització de l’estat i la situació del jovent, majoritari a la població ivoriana. Malauradament, les dificultats prèvies a les eleccions és van agreujar amb un resultat ajustat i la proclamació dels dos candidats com a guanyadors. Aquest desafortunat escenari ha fet encara més valuosa la present relatoria.

The role of basalt replenishment in the generation of basaltic andesites of the ongoing activity at Arenal volcano, Costa Rica: evidence from clinopyroxene and spinel

The bulk composition of magma erupted from Volcan Arenal has remained nearly constant (SiO2 = 53.6-54.9 wt%; MgO = 5.0-4.5 wt%) during almost 30 years of continuous activity (1969-1996). None the less, clinopyroxene (cpx) phenocrysts and their spinel inclusions record a much more complex open-system evolution in which steady-state production of the erupted basaltic andesitic magma is linked to episodic injections of basalt into Arenal's magma conduit/reservoir system. High-resolution major element zoning profiles (electron microprobe) on a large number of phenocrysts (>14,000 analyses), tied to back-scattered electron (BSE) images, have been used to assess the compositional characteristics of the magmatic end members as well as the timing and dynamics of magma replenishment events. No two cpx phenocrysts have exactly the same zoning profile. The vast majority of our analyses record the crystallization of cpx (Cr2O3 < 0.12 wt%; Mg# = 65-79; Al/Ti = 2-7) from a liquid comparable to or more evolved than erupted magma compositions. However, half of all cpx grains are cored by high-Cr cpx (Cr2O3 = 0.2-0.72 wt%) or contain similar basaltic compositions as abrupt growth bands in phenocrysts with and without high-Cr cores; phenocrysts with high-Cr cpx occur throughout the ongoing activity. In a few cases, high-Cr cpx occurs very near the outer margin of the grain without an apparent growth hiatus, particularly in 1968/69 and 1992/93. The main conclusions are: (1) all basaltic andesitic lavas erupted at Arenal during the ongoing activity that began in July, 1968, are the products of magma mixing, (2) clinopyroxenes record multiple replenishment events of basaltic magma in contrast to the near constancy of erupted bulk compositions, (3) some phenocrysts preserve records of multiple interactions with basaltic magmas requiring magmatic processes to operate on time-scales shorter than residence times of some phenocrysts, (4) multiple occurrences of clinopyroxene with high-Cr rims suggest that basalt replenishment events have occurred with sub-decadal frequency and may predate eruption by months or less. From this we infer that Arenal volcano is underlain by a continuously active, small-volume magmatic reservoir maintained in quasi-steady state by basalt recharge over several decades. The monotony of erupting Arenal magmas implies that fractionation, recharge, ascent, and eruption are well balanced in order for magmas to be essentially uniform while containing phenocrysts with vastly different growth histories at the time of eruption.

Zymodeme and Serodeme Characterization of Leishmania Isolates Obtained from Costa Rican Patients

Thirty-four Leishmania isolates obtained from Costa Rican patients, from different geographical areas, were characterized by isoenzyme electrophoresis and indirect immunofluorescense with monoclonal antibodies. Thirty-two were characterized as L. panamensis strains and two were L. braziliensis variants. We confirm the evident predominance of L. panamensis as the main etiological agent of leishmaniasis in Costa Rica and the existence of L. braziliensis in the country.