An integrated biostratigraphy (conodonts and foraminifers) and chronostratigraphy (paleomagnetic reversals, magnetic susceptibility, elemental chemistry, carbon isotopes and geochronology) for the Permian-Triassic strata of Guandao section

The chronostratigraphy of Guandao section has served as the foundation for numerous studies of the end-Permian extinction and biotic recovery in south China. Guandao section is continuous from the Permian-Triassic boundary to the Upper Triassic.Conodonts enable broad delineation of stage and substage boundaries and calibration of foraminifer biostratigraphy as follows. Changhsingian- Griesbachian: first Hindeodus parvus, and first appearance of foraminifers Postcladella kalhori and Earlandia sp. Griesbachian-Dienerian: first Neospathodus dieneri, and last appearance of foraminifer P. grandis. Dienerian-Smithian: first Novispathodus waageni and late Dienerian first appearance of foraminifer Hoyenella ex gr. sinensis. Smithian-Spathian: first Nv? crassatus and last appearance of foraminifers Arenovidalina n. sp. and Glomospirella cf. vulgaris. Spathian-Aegean: first Chiosella timorensis and first appearance of foraminifer Meandrospira dinarica. Aegean-Bithynian: first Nicoraella germanica and first appearance of foraminifer Pilammina densa. Bithynian-Pelsonian: after last Neogondolella regalis, prior to first Paragondolella bulgarica and first appearance of foraminifer Aulotortus eotriasicus. Pelsonian-Illyrian: first Pg. excelsa and last appearance of foraminifers Meandrospira ? deformata and Pilamminella grandis. Illyrian-Fassanian: first Budurovignathus truempyi, and first appearance of foraminifers Abriolina mediterranea and Paleolituonella meridionalis. Fassanian-Longobardian: first Bv. mungoensis and last appearance of foraminifer A. mediterranea. Longobardian-Cordevolian: first Quadralella polygnathiformis and last appearance of foraminifers Turriglomina mesotriasica and Endotriadella wirzi. The section contains primary magnetic signature with frequent reversals occurring around the Permian-Triassic, Olenekian-Anisian, and Anisian-Ladinian boundaries. Predominantly normal polarity occurs in the lower Smithian, Bithynian, and Longobardian-Cordevolian. Predominantly reversed polarity occurs in the upper Griesbachian, Induan-Olenekian, Pelsonian and lower Illyrian. Reversals match well with the GPTS. Large amplitude carbon isotope excursions, attaining values as low as -2.9 per mil d13C and high as +5.7 per mil d13C, characterize the Lower Triassic and basal Anisian. Values stabilize around +2 per mil d13C through the Anisian to Carnian. Similar signatures have been reported globally. Magnetic susceptibility and synthetic gamma ray logs show large fluctuations in the Lower Triassic and an overall decline in magnitude of fluctuation through the Middle and Upper Triassic. The largest spikes in magnetic susceptibility and gamma ray, indicating greater terrestrial lithogenic flux, correspond to positive d13C excursions. Several volcanic ash horizons occur in the Lower Triassic and Olenekian-Anisian boundary. High resolution U-Pb analysis of zircons provide a robust age of 247.2 Ma for the Olenekian-Anisian boundary.

Modeled integrated responses of ppERK1 and ppERK2 of normal and elevated ERK1 and ERK2 levels (Figure 5b)

The present dataset contain source data for Figure 5b from Schilling et al., 2009. Cell fate decisions are regulated by the coordinated activation of signalling pathways such as the extracellular signal-regulated kinase (ERK) cascade, but contributions of individual kinase isoforms are mostly unknown. The authors combined quantitative data from erythropoietin-induced pathway activation in primary erythroid progenitor (colony-forming unit erythroid stage, CFU-E) cells with mathematical modelling, in order to predict and experimentally confirmed a distributive ERK phosphorylation mechanism in CFU-E cells. The authors found evidences that double-phosphorylated ERK1 attenuates proliferation beyond a certain activation level, whereas activated ERK2 enhances proliferation with saturation kinetics. They show integrated responses of double-phosphorylated ERK1 and ERK2 that were calculated for different Epo concentrations for the original model as well as for models with elevated ERK1 or ERK2 levels.

Integrated Paleocene calcareous plankton magnetobiochronology of DSDP Hole 43-384 in the Northwest Atlantic Ocean

At Deep Sea Drilling Site 384 (J-Anomaly Ridge, Grand Banks Continental Rise, NW Atlantic Ocean) Paleocene nannofossil chalks and oozes (~70 m thick) are unconformably/disconformably underlain (~168 m; upper Maastrichtian) and overlain (~98.7 m; upper lower Eocene) by sediments of comparable lithologies. The chalks are more indurated in stratigraphically higher levels of the Paleocene reflecting increasing amounts of biosiliceous (radiolarians and diatoms) components. This site serves as an excellent location for an integrated calcareous and siliceous microfossil zonal stratigraphy and stable isotope stratigraphy. We report the results of a magnetostratigraphic study which, when incorporated with published magnetostratigraphic results, reveals an essentially complete magnetostratigraphic record spanning the interval from Magnetochron C31n (late Maastrichtian) to C25n (partim) (late Paleocene, Thanetian). Integrated magnetobiochronology and stable isotope stratigraphy support the interpretation of, and constrain the estimated duration of, a short hiatus (~0.9 my) within the younger part of Chron C29r (including the K/P boundary) and an ~6 my hiatus separating upper Paleocene (Magnetozone C25n) and upper lower Eocene (Magnetozone C22r) sediments. Some 30 planktonic foraminiferal datum levels [including the criteria used to denote the Paleocene planktonic foraminiferal (sub)tropical zonal scheme of Berggren and Miller, Micropaleontology 34 (4) (1988) 362-380 and Berggren et al., SEPM Spec. Publ. 54 (1995) 129-212, Geol. Soc. Am. Bull. 107 (11) (1995) 1272-1287], and nearly two dozen calcareous nannoplankton datum levels have been recognized and calibrated to the magnetochronology. Planktonic foraminiferal Subzones P4a and P4b of (upper Paleocene) Zone P4 are emended/redefined based on the discovery of a longer stratigraphic extension of Acarinina subsphaerica (into at last Magnetozone C25n). Stable isotope stratigraphies from benthic foraminifera and fine fraction (<38 µm) carbonate have been calibrated to the biochronology and magnetostratigraphy. A minimum in benthic foraminifer delta13C was reached near the Danian/Selandian boundary (within Chron C26r, planktonic foraminiferal Zone P3a and calcareous nannoplankton Zone NP4) and is followed by the rise to maximum delta13C values in the late Thanetian (near the base of C25n, in Zone P4c and NP9a, respectively) that can be used for global correlation in the Paleocene.

(Table 1) Trace element and age analysis from the Porites coral from IODP Exp 310

Climate responses and changes in marine environments during the last deglaciation have been controversial and few paleoceanographic data are available from the tropical South Pacific, though this region is crucial in the investigations of ocean-atmosphere interactions. Integrated Ocean Drilling Program Expedition 310 was conducted to establish the time course of the postglacial sea-level rise at Tahiti in the South Pacific. A principal objective of this expedition was to examine the variation of marine environments during the last deglaciation. As fossil Porites coral is ideal for assessing past marine environments, we selected only Porites specimens from the many coral specimens retrieved, examined them by XRD, and dated them by the 14C method. In all, we obtained 17 pristine Porites specimens composed of only aragonite with ages from 15 to 9 ka. Then, we measured Mg/Ca, Ba/Ca, and U/Ca ratios and Cd contents as proxies for upwelling and sea surface temperature. Higher Ba/Ca ratios and Cd content together with lower reconstructed SSTs using U/Ca ratios in the coral specimens between 12.6 and 9.8 cal ka compared to around 15 cal ka suggest that upwelling and/or entrainment of subsurface water into mixed layer was enhanced around Tahiti during this period. This finding is consistent with previous reports and supports the idea that the South Pacific was characterized by La Niña-like conditions at least from 12.6 to 9.8 cal ka.

(Table 1) Different age analysis from IODP Site 310-M0018A

Deglacial reefs from Tahiti (IODP 310) feature a co-occurrence of zooxanthellate corals with microbialites that compose up to 80 vol% of the reef framework. The notion that microbialites tend to form in more nutrient-rich environments has previously led to the concept that such encrustations are considerably younger than the coral framework, and that they have formed in deeper storeys of the reef edifice, or that they represent severe disturbances of the reef ecosystem. As indicated by their repetitive interbedding with coralline red algae, the microbialites of this reef succession of Tahiti, however, formed immediately after coral growth under photic conditions. Clearly, the deglacial reef microbialites present in the IODP 310 cores did not follow disturbances such as drowning or suffocation by terrestrial material, and are not "disaster forms". Given that the corals and the microbialites developed in close spatial proximity, highly elevated nutrient levels caused by fluvial or groundwater transport from the volcanic hinterland are an unlikely cause for the exceptionally voluminous development of microbialites. That voluminous deglacial reef microbialites generally are restricted to volcanic islands, however, implies that moderately, and possibly episodically elevated nutrient levels favored this type of microbialite formation.

Integrated values of heterotrophic picoplankton and primary production (euphotic zone) in the Antarctic polar frontal region during POLARSTERN cruises ANT-XIII/2 and ANT-XVI/3

We assessed relationships between phytoplankton standing stock, measured as chlorophyll a (Chl a), primary production (PP), and heterotrophic picoplankton production (HPP), in the epipelagic zone (0-100 m) as well as in the mesopelagic zone (100-1,000 m) in the polar frontal zone of the Atlantic sector of the Southern Ocean in austral summer (late December to January) and fall (March to early May). Integrated epipelagic HPP was positively correlated to integrated PP in summer (data for fall are not available) but not to integrated Chl a. However, integrated mesopelagic HPP was positively correlated to Chl a in summer as well as fall. The mesopelagic fraction of HPP as a percentage of total HPP was also positively correlated to Chl a, whereas the epipelagic fraction of HPP was negatively correlated to it. These results indicate that with increasing phytoplankton standing stock, constituted mainly of highly silicified diatoms, the focus of its consumption by heterotrophic picoplankton shifts from epipelagic to mesopelagic waters. With a growth efficiency of 30%, our HPP data indicate that in both the epipelagic and mesopelagic zone heterotrophic picoplankton consume 20% of PP. Mesopelagic heterotrophic picoplankton consumed around 80% of the sinking flux, measured from depletion of 234Th, which is a lower fraction than that reported from the central and subarctic Pacific. Our analysis indicates that it is important to include mesopelagic HPP in comprehensive assessments of the microbial consumption of PP, phytoplankton biomass, and particulate organic matter in cold oceanic systems with high rates of export production.

Metadata und statistic analysis of archael and bacterial sequences originating from sediments of the Håkon Mosby mud volcano (Z1-Z3, all habitats)

DNA extraction was carried out as described on the MICROBIS project pages (http://icomm.mbl.edu/microbis ) using a commercially available extraction kit. We amplified the hypervariable regions V4-V6 of archaeal and bacterial 16S rRNA genes using PCR and several sets of forward and reverse primers (http://vamps.mbl.edu/resources/primers.php). Massively parallel tag sequencing of the PCR products was carried out on a 454 Life Sciences GS FLX sequencer at Marine Biological Laboratory, Woods Hole, MA, following the same experimental conditions for all samples. Sequence reads were submitted to a rigorous quality control procedure based on mothur v30 (doi:10.1128/AEM.01541-09) including denoising of the flow grams using an algorithm based on PyroNoise (doi:10.1038/nmeth.1361), removal of PCR errors and a chimera check using uchime (doi:10.1093/bioinformatics/btr381). The reads were taxonomically assigned according to the SILVA taxonomy (SSURef v119, 07-2014; doi:10.1093/nar/gks1219) implemented in mothur and clustered at 98% ribosomal RNA gene V4-V6 sequence identity. V4-V6 amplicon sequence abundance tables were standardized to account for unequal sampling effort using 1000 (Archaea) and 2300 (Bacteria) randomly chosen sequences without replacement using mothur and then used to calculate inverse Simpson diversity indices and Chao1 richness (doi:10.2307/4615964). Bray-Curtis dissimilarities (doi:10.2307/1942268) between all samples were calculated and used for 2-dimensional non metric multidimensional scaling (NMDS) ordinations with 20 random starts (doi:10.1007/BF02289694). Stress values below 0.2 indicated that the multidimensional dataset was well represented by the 2D ordination. NMDS ordinations were compared and tested using Procrustes correlation analysis (doi:10.1007/BF02291478). All analyses were carried out with the R statistical environment and the packages vegan (available at: http://cran.r-project.org/package=vegan), labdsv (available at: http://cran.r-project.org/package=labdsv), as well as with custom R scripts. Operational taxonomic units at 98% sequence identity (OTU0.03) that occurred only once in the whole dataset were termed absolute single sequence OTUs (SSOabs; doi:10.1038/ismej.2011.132). OTU0.03 sequences that occurred only once in at least one sample, but may occur more often in other samples were termed relative single sequence OTUs (SSOrel). SSOrel are particularly interesting for community ecology, since they comprise rare organisms that might become abundant when conditions change.16S rRNA amplicons and metagenomic reads have been stored in the sequence read archive under SRA project accession number SRP042162.