Proteomics of the neurotoxic fraction from the sea anemone Bunodosoma cangicum venom: Novel peptides belonging to new classes of toxins

In contrast to the many studies on the venoms of scorpions, spiders, snakes and cone snails, tip to now there has been no report of the proteomic analysis of sea anemones venoms. In this work we report for the first time the peptide mass fingerprint and some novel peptides in the neurotoxic fraction (Fr III) of the sea anemone Bunodosoma cangicum venom. Fr III is neurotoxic to crabs and was purified by rp-HPLC in a C-18 column, yielding 41 fractions. By checking their molecular masses by ESI-Q-Tof and MALDI-Tof MS we found 81 components ranging from near 250 amu to approximately 6000 amu. Some of the peptidic molecules were partially sequenced through the automated Edman technique. Three of them are peptides with near 4500 amu belonging to the class of the BcIV, BDS-I, BDS-II, APETx1, APETx2 and Am-II toxins. Another three peptides represent a novel group of toxins (similar to 3200 amu). A further three molecules (similar to similar to 4900 amu) belong to the group of type 1 sodium channel neurotoxins. When assayed over the crab leg nerve compound action potentials, one of the BcIV- and APETx-like peptides exhibits an action similar to the type 1 sodium channel toxins in this preparation, suggesting the same target in this assay. On the other hand one of the novel peptides, with 3176 amu, displayed an action similar to potassium channel blockage in this experiment. In summary, the proteomic analysis and mass fingerprint of fractions from sea anemone venoms through MS are valuable tools, allowing us to rapidly predict the occurrence of different groups of toxins and facilitating the search and characterization of novel molecules without the need of full characterization of individual components by broader assays and bioassay-guided purifications. It also shows that sea anemones employ dozens of components for prey capture and defense. (C) 2008 Elsevier Inc. All rights reserved.

ON THE IDENTITY OF CYCLORAMPHUS JORDANENSIS HEYER, 1983 (ANURA: CYCLORAMPHIDAE)

Cycloramphus jordanensis was described based on a single preserved specimen from Campos do Jordao (22 degrees 44` S ,45 degrees 35` W), State of Sao Paulo, Brazil. While examining the holotype; we noticed the presence of toe and tarsal fringes. Because these characters are absent in Cycloramphus, we suspected that the species was mistakenly placed in the genus. X-ray images of the holotype revealed T-shaped terminal phalanges and fang-like teeth. Together with the presence of toe and tarsal fringes, these characters squarely place the specimen in the genus Megaelasia. Considering the striking niche differences between Cycloramphus and Megaclosia, we expect the new combination will facilitate location of new individuals of this rare frog.

THE ROLE OF INNATE IMMUNITY IN SEPTIC ACUTE KIDNEY INJURIES

Acute kidney injury (AKI) is an important clinical syndrome characterized by abnormalities in the hydroelectrolytic balance. Because of high rates of morbidity and mortality (from 15% to 60%) associated with AKI, the study of its pathophysiology is critical in searching for clinical targets and therapeutic strategies. Severe sepsis is the major cause of AKI. The host response to sepsis involves an inflammatory response, whereby the pathogen is initially sensed by innate immune receptors (pattern recognition receptors [PRRs]). When it persists, this immune response leads to secretion of proinflammatory products that induce organ dysfunction such as renal failure and consequently increased mortality. Moreover, the injured tissue releases molecules resulting from extracellular matrix degradation or dying cells that function as alarmines, which are recognized by PRR in the absence of pathogens in a second wave of injury. Toll-like receptors (TLRs) and NOD-like receptors (NLRs) are the best characterized PRRs. They are expressed in many cell types and throughout the nephron. Their activation leads to translocation of nuclear factors and synthesis of proinflammatory cytokines and chemokines. TLRs` signaling primes the cells for a robust inflammatory response dependent on NLRs; the interaction of TLRs and NLRs gives rise to the multiprotein complex known as the inflammasome, which in turn activates secretion of mature interleukin 1 beta and interleukin 18. Experimental data show that innate immune receptors, the inflammasome components, and proinflammatory cytokines play crucial roles not only in sepsis, but also in organ-induced dysfunction, especially in the kidneys. In this review, we discuss the significance of the innate immune receptors in the development of acute renal injury secondary to sepsis.

The concept of quasi-integrability: a concrete example

We use the deformed sine-Gordon models recently presented by Bazeia et al [1] to take the first steps towards defining the concept of quasi-integrability. We consider one such definition and use it to calculate an infinite number of quasi-conserved quantities through a modification of the usual techniques of integrable field theories. Performing an expansion around the sine-Gordon theory we are able to evaluate the charges and the anomalies of their conservation laws in a perturbative power series in a small parameter which describes the ""closeness"" to the integrable sine-Gordon model. We show that in the case of the two-soliton scattering the charges, up to first order of perturbation, are conserved asymptotically, i.e. their values are the same in the distant past and future, when the solitons are well separated. We indicate that this property may hold or not to higher orders depending on the behavior of the two-soliton solution under a special parity transformation. For closely bound systems, such as breather-like field configurations, the situation however is more complex and perhaps the anomalies have a different structure implying that the concept of quasi-integrability does not apply in the same way as in the scattering of solitons. We back up our results with the data of many numerical simulations which also demonstrate the existence of long lived breather-like and wobble-like states in these models.

Kraftutveckling för manuella trädfällningsredskap : Force development for manual tree felling tools

The objective with this study has been to build general models of the mechanics in tree felling with chain-saw and to compare felling torque for different tools. The theoretical models are completed and validated with a comparative study. The study includes a great number of felling tools of which some are used with different methods. Felling torque was measured using a naturally like measuring arrangement where a tree is cut at about 3.7 m height and then anchored with a dynamometer to a tree opposite to the felling direction. Notch and felling cut was made as ordinary with exception that the hinge was made extra thin to reduce bending resistance. The tree was consequently not felled during the trials and several combinations of felling tools and individuals could be used on the same tree.The results show big differences between tools, methods and persons. The differences were, however, not general, but could vary depending on conditions (first of all tree diameters). Tools and methods that push or pull on the stem are little affected by the size of the tree, while tools that press on the stump are very much dependent of a large stump-diameter. Hand force asserted on a simple pole is consequently a powerful tool on small trees. For trees of medium size there are several alternative methods with different sizes and brands of felling levers and wedges. Larger and more ungainly tools and methods like tree pusher, winch, etc. develop very high felling torque on all tree sizes. On large trees also the felling wedge and especially the use of several wedges together develop very high felling torque.

Provningsmetod för integrerade sol-biosystem : Årsverkningsgrad genom korttidsmätning

Test method for integrated solar- biomass systems - Long term prediction trough short term measurementsSP Technical Research Institute of Sweden and SERC, Dalarna University have in cooperation developed a test method for integrated solar and biomass systems. The test method is performed under six days including two summer days, two winter days and two spring/autumn days true to real weather conditions and loads for a single family house. The aim of the test method is to get information about a Combisystem’s annual performance and operation throughout a short term test. Seven different solar Combisystems have been tested within the project together with a pellet boiler without solar collectors. In addition to that a comparative testing has been performed at the two laboratories at SP and at SERC on the same Combisystem. The test method developed within the project has been proved to withstand the aim of the project, which is to be able to compare the performance between the systems. The test method is also suitable for identification of possible operation problems so they can be taken care of and consequently improves the system.The project and the system testing reveal that it is in general favorable to combine biomass pellets with solar heating. Pellet boilers has normally a low performance during the summer period but combined with a solar collector the boiler can be switch off during this period. There are however big differences in performance between the tested. The efficiency of the pellet boiler is highly dependent of the operating conditions and elements like heat losses from the system, system configuration and control strategy have great influence of the performance of the system and the emissions. On the other hand, the performance and the size of the solar collectors have a minor effect on the overall system performance. There is obviously a big potential for improvement of the system´s performance and the developed test method is an essential way to implement this perfection.

Soft computing in biometrics : a pragmatic appraisal

The ever increasing spurt in digital crimes such as image manipulation, image tampering, signature forgery, image forgery, illegal transaction, etc. have hard pressed the demand to combat these forms of criminal activities. In this direction, biometrics - the computer-based validation of a persons' identity is becoming more and more essential particularly for high security systems. The essence of biometrics is the measurement of person’s physiological or behavioral characteristics, it enables authentication of a person’s identity. Biometric-based authentication is also becoming increasingly important in computer-based applications because the amount of sensitive data stored in such systems is growing. The new demands of biometric systems are robustness, high recognition rates, capability to handle imprecision, uncertainties of non-statistical kind and magnanimous flexibility. It is exactly here that, the role of soft computing techniques comes to play. The main aim of this write-up is to present a pragmatic view on applications of soft computing techniques in biometrics and to analyze its impact. It is found that soft computing has already made inroads in terms of individual methods or in combination. Applications of varieties of neural networks top the list followed by fuzzy logic and evolutionary algorithms. In a nutshell, the soft computing paradigms are used for biometric tasks such as feature extraction, dimensionality reduction, pattern identification, pattern mapping and the like.

Local politico-administrative perspectives on quality improvement based on national registry data in Sweden : a qualitative study using the Consolidated Framework for Implementation Research.

Background. Through a national policy agreement, over 167 million Euros will be invested in the Swedish National Quality Registries (NQRs) between 2012 and 2016. One of the policy agreement¿s intentions is to increase the use of NQR data for quality improvement (QI). However, the evidence is fragmented as to how the use of medical registries and the like lead to quality improvement, and little is known about non-clinical use. The aim was therefore to investigate the perspectives of Swedish politicians and administrators on quality improvement based on national registry data. Methods. Politicians and administrators from four county councils were interviewed. A qualitative content analysis guided by the Consolidated Framework for Implementation Research (CFIR) was performed. Results. The politicians and administrators perspectives on the use of NQR data for quality improvement were mainly assigned to three of the five CFIR domains. In the domain of intervention characteristics, data reliability and access in reasonable time were not considered entirely satisfactory, making it difficult for the politico-administrative leaderships to initiate, monitor, and support timely QI efforts. Still, politicians and administrators trusted the idea of using the NQRs as a base for quality improvement. In the domain of inner setting, the organizational structures were not sufficiently developed to utilize the advantages of the NQRs, and readiness for implementation appeared to be inadequate for two reasons. Firstly, the resources for data analysis and quality improvement were not considered sufficient at politico-administrative or clinical level. Secondly, deficiencies in leadership engagement at multiple levels were described and there was a lack of consensus on the politicians¿ role and level of involvement. Regarding the domain of outer setting, there was a lack of communication and cooperation between the county councils and the national NQR organizations. Conclusions. The Swedish experiences show that a government-supported national system of well-funded, well-managed, and reputable national quality registries needs favorable local politico-administrative conditions to be used for quality improvement; such conditions are not yet in place according to local politicians and administrators.

Memórias de trabalhadores do Vital Brazil: um diálogo entre a saúde e a comunidade

O estudo teve como proposta identificar na comunidade do Morro do Vital Brazil referências que indicassem sentido de coletividade e continuidade. Essa comunidade, como pretendido apontar, teria sua origem, nas décadas de 1920 e 1930, no entorno do Instituto de Hygiene, Sorotherapia e Veterinária do Estado do Rio de Janeiro, hoje, Instituto Vital Brazil. Por ser fábrica farmacêutica, a produção necessitaria de mão-de-obra, que no caso estudado, passou a morar no morro atrás do Instituto, mas ainda em seu território. Dessa origem, surge uma comunidade com características de cooperação, união e associativismo. Com a prosperidade da fábrica, cresce o número de moradores e inicia-se um conjunto de domicílios e famílias também possuidores de aspectos em comum. Essas identidades possibilitam um encontro com o poder público na forma de políticos e políticas, como o Programa Médico de Família. Com esse último, interesse inicial da pesquisa, nasceu a relação entre a pesquisadora em questão, médica no posto PMF Vital Brazil, e evidenciou performances dos moradores que indicavam um pertencimento e lugar de fala diferenciado. O estudo apontou características da comunidade e dos atores que contribuíram na criação desse coletivo, utilizou como metodologia a história oral.

Gestão estratégica da informação como fator condicionante para a definição e implementação de políticas de defesa e segurança nacional no contexto da Amazônia Legal: o caso SIPAM/SIVAM

The objective of this work is the study of the existing correlations between the strategical use of the information and the joint and implementation of defense politics and national security in the Legal Amazonian. For in such a way, the proposal was developed from the analysis of the systems of protection and monitoring of the Amazonian (SIPAM/SIVAM), where we search to inquire as these systems have contributed for the definition and implantation of these politics. For the Amazonian, with its natural wealth, threats and vulnerabilities, the perspectives of integration, security and national defense and of sustainable development constitute great challenges to be faced, where the efficient use of the technology is a basic reference that must be incorporated in the strategies and public politics in these areas. One is about a strategical project, conceived with vision of future, protection and development of the . The objective SIPAM/SIVAM the defense and the guarantee of the Brazilian sovereignty in the Legal Amazonian, beyond the systematization and accomplishment of the governmental actions in the region, by means of the intensive use of technological apparatus. In turn, they reflect the priority that the Amazon region has in terms of defense and security for the Country, and symbolize the strategy of the State to protect it. The SIPAM/SIVAM if finds in a boarding line in which the guarantee of the national sovereignty also involves the care with the development of the local population, inside of a proposal educative and integrator. Like conclusion we affirm that of the SIPAM/SIVAM creates a new paradigm for the public administration, where the organizations work with a shared set of information, beyond starting to act of integrated form. Thus, when searching permanently the rationalization of efforts and resources, trying an unknown form of institution relationship where infrastructure and products are shared, the SIPAM/SIVAM creates a new premise for the Brazilian public administration and contributes to give a new direction to the development of the Amazonian.

Os requisitos individuais da empregabilidade no sistema nacional de emprego - SINE - RJ

O estudo em tela buscou verificar o nível de exigência das organizações no que se refere aos requisitos individuais dos candidatos a emprego, intermediados pelo SINE/RJ, nos processos de recrutamento promovidos no universo de atuação da SETRAB - Secretaria de Estado do Trabalho do Rio de Janeiro. Este meio foi utilizado por ser o SINE/RJ o principal e mais importante instrumento de encaminhamento de candidatos a emprego do Estado. O fato de haver necessidade de promover este tipo de serviço, torna o SINE/RJ um importante órgão social e fonte significativa de dados para pesquisas desta natureza. Verificou-se que certos requisitos permanecem valorizados, como escolaridade e experiência por exemplo, e outros surgem como novidade demonstrando mudança - morar próximo ao local de trabalho e ser jovem. Além destes aspectos, verificou-se que há diversas correntes de pensamento sobre as causas do desemprego, de dificultadores da empregabilidade. Assim, também se pode considerar que as organizações valorizam o cargo mais flexível e que a depender do nível, falar inglês e dominar a informática podem, de certa forma, favorecer o aproveitamento do candidato, ou seja, certa corrente de pensamento aponta para a qualificação como forma de vencer a escassez de vagas. Já outros afirmam que o problema é conjuntural e, a não ser que se modifique a estrutura econômica, será difícil empregar todos os que procuram trabalho. Uma terceira corrente afirma que o momento é de transição e que a "mão invislvel do mercado logo ajustará as coisas". Afirmam estes que a tecnologia acaba com empregos arcaicos, mas cria outros na mesma proporção. São então pontos de vista que na verdade são colocados de acordo com a ideologia dos estudiosos. São hipóteses aceitas por certos setores específicos da mídia e daqueles que buscam respostas para o que ora aflige, e isso é um fato, grande parcela da sociedade brasileira. No caso específico do SINE/RJ, órgão governamental, a expectativa é que a partir do conhecimento factual da realidade, possam ser empreendi- das ações no sentido de favorecer à colocação da mão-de-obra ociosa e ávida por uma oportunidade de emprego. A Secretaria Estadual do Trabalho promove ações de treinamento e qualificação de pessoal com o intuito de facilitar o acesso de desempregados a cargos que, muitas vezes, deixam de ser preenchidos por falta de pessoas preparadas para ocupá-los. Pode-se concluir que dentre as diversas formas de ver o problema, há na verdade uma certa concorrência de causas para o estabelecimento da situação atual. O fato é que algo precisa ser feito para amenizar o que DEJOURS (1999) denomina de "Banalização da Injustiça Sociar e, por ser uma quarta corrente, também deve merecer a atenção das autoridades, em ações sociais facilitadoras do acesso e manutenção das pessoas no mercado de trabalho. Esta corrente (que além de DEJOURS, tem outros adeptos), trata o assunto como sendo algo não aleatório mas intencional. Para esta linha de pensamento, o desemprego é algo deliberado, com intuitos econômicos, além de uma certa dose de crueldade social. Enfim, espera-se que os dirigentes sejam sensibilizados e possa haver solução para este tipo de dificuldade social.

A responsabilidade social como resposta do sistemas ao ambiente institucional brasileiro pós-década de 1990: O caso do Sesc

Analysis of the influences exerted by the Brazilian institutional scenario on SESC ¿ The Social Service of Commerce, focusing state-market-society relations in the 1990 decade, states the core issue of the present dissertation. It also aims to discuss the suitability of adopting corporate social responsibility oriented actions in accordance with these changes. In the first place SESC's institutional setting, and questions addressed to it, as well as pressures to which the organization has been submitted since the mid 1980's are presented to the reader. Following, elements of particular significance closely related to SESC's present institutional framework are described: the country's political redemocratization, social and organizational networks, state reform, and the like.

Parque Nacional Serra da Capivara: patrimônio cultural da humanidade

O presente trabalho tem como objetivo analisar o Parque Nacional Serra da Capivara desde sua criação à situação atual a fim de compreender as razões que até hoje impedem o seu pleno funcionamento. Para tanto, foram analisados aspectos anteriores à constituição do Parque, desde as primeiras expedições da Missão Franco-Brasileira no Piauí, até itens como infraestrutura, equipamentos e potencial turístico oferecidos pelo Parque atualmente. Aliado a esse estudo, foram colhidos depoimentos, entre eles, de alguns dos atores diretamente envolvidos no desenvolvimento do Parque. O resultado é um panorama histórico que explica as circunstâncias que fazem do Parque Nacional Serra da Capivara Patrimônio Cultural da Humanidade.

Indicadores de produtividade aplicados à criminalística da Polícia Federal

A avaliação do desempenho das instituições públicas por meio de indicadores tem propiciado uma grande mudança no comportamento dos gestores governamentais. Este trabalho apresenta a pesquisa realizada para definição de indicadores de produtividade para o Instituto Nacional de Criminalística (INC) da Polícia Federal. Foi realizado um levantamento bibliográfico sobre indicadores de desempenho em empresas, em instituições públicas e afins, oferecendo uma proposta de indicadores que possibilitem avaliar a atuação do INC em termos de eficiência. A pesquisa de campo foi realizada entre peritos criminais federais para verificar suas percepções sobre indicadores atuais e quais seriam os ideais. Posteriormente, foi feita uma análise dos dados armazenados no Sistema de Criminalística (SISCRIM) a fim de avaliar a adoção de tais indicadores.

Gestão do conhecimento na administração pública e uso de tecnologia da informação para melhoria dos resultados: o caso do Corpo de Intendentes da Marinha

Vivemos em uma sociedade cada vez mais globalizada, atenta, informada e exigente com os serviços prestados pelas organizações públicas e privadas. A tecnologia disponibiliza a um número cada vez maior de pessoas, em tempo real, um conjunto enorme de informações, opiniões, possibilitando que elas interajam com diferentes culturas e, desconsiderando suas distâncias, as unem de forma nunca antes experimentada pela humanidade. A influência da tecnologia vem trazendo consequências tão profundas ao nosso estilo de vida que, a cada nova invenção, novas experiências podem ser vividas e palavras como inovação, reinvenção e mudança estão constantemente em pauta. Nesse contexto é que a gestão do conhecimento vem se tornando cada vez mais importante nos ambientes corporativos, unindo pessoas certas, desenvolvendo e compartilhando novos conhecimentos, enfim, tornando mais fácil lidar com as constantes mudanças e consequentes inseguranças geradas. Assim, este trabalho verificou qual o nível de gestão do conhecimento realizado dentro de um órgão da administração pública federal brasileira, a Marinha do Brasil, e, especificamente no seu Corpo de Intendentes, mediu em oito de suas principais organizações militares, por meio de métodos qualitativos e quantitativos, como essa ferramenta vem sendo utilizada. Ao final, de forma propositiva, com base nos resultados das avaliações realizadas e na revisão teórica, foi sugerida uma estrutura de gestão do conhecimento que possibilite melhorar os atuais níveis de gestão do conhecimento detectados, contribuindo para que ações empreendidas nesse sentido sejam mais eficazes, eficientes e efetivas, acelerando, portanto, o desenvolvimento dessas organizações.