Estudio del rendimiento bajo presión en los tiros libres en baloncesto, y su influencia sobre el porcentaje de acierto durante el último minuto de juego

El minuto final de un partido ajustado de baloncesto es un momento crítico que está sujeto a multitud de factores que influyen en su desarrollo. Así, el porcentaje de acierto en los tiros libres durante ese periodo de tiempo va a determinar, en muchas ocasiones, el resultado final del partido. La disminución de rendimiento (drop) en esta faceta de juego en condiciones de presión, puede estar relacionada con múltiples variables propias del contexto deportivo estudiado, como por ejemplo: los segundos restantes de posesión, la situación en el marcador (ir ganando, empatando o perdiendo), la localización del partido (jugar en casa o fuera), la fase de competición (fase regular o eliminatorias) o el nivel del equipo (mejores/peores equipos). Además, las características del jugador que realiza los lanzamientos tienen una gran importancia respecto a su edad y años de experiencia para afrontar los momentos críticos, así como el puesto de juego que ocupa en el equipo. En este sentido, la combinación de factores del contexto y del jugador, permiten interactuar en el rendimiento del lanzador en los momentos finales de partido durante sus lanzamientos de tiro libre. El presente trabajo de tesis doctoral tiene como objetivo encontrar aquellas variables más relacionadas con la disminución de rendimiento del jugador en los tiros libres durante el último minuto de juego, y la última serie de tiros libres en los partidos ajustados de baloncesto. Para alcanzar el objetivo del estudio se analizaron 124 partidos ajustados (diferencias iguales o inferiores a 2 puntos) de todas las competiciones (fase regular, playoff y copa del Rey) de la liga ACB durante las temporadas 2011-2012 a 2014-2015. Para el registro de variables se analizó el porcentaje de acierto en los tiros libres del lanzador en la liga regular, partido completo, último minuto y última serie. De este modo se trató de analizar qué variables del contexto y del jugador permitían explicar el rendimiento en los tiros libres durante el último minuto, y la última serie de tiros libres del partido. Por otro lado, se trató de conocer el grado de asociación entre el descenso del rendimiento (drop) en los momentos finales de partido, y las variables estudiadas del jugador: puesto de juego, edad, y años de experiencia profesional; mientras que las variables situacionales consideradas fueron: fase de competición, localización, clasificación, tiempo restante, y diferencia parcial en el marcador. Para el análisis de los datos se realizaron dos modelos estadísticos: 1º) un modelo de regresión lineal múltiple para conocer el efecto de las variables independientes en el porcentaje de aciertos del lanzador en el último minuto, y en la última serie de tiros libres del partido; y 2º) un análisis de regresión logística binomial para analizar la relación existente entre la probabilidad de tener un drop (disminución del rendimiento) y las características del lanzador, y las variables situacionales. Los resultados del modelo de regresión lineal múltiple mostraron efectos negativos significativos en el porcentaje de acierto en los tiros libres durante el último minuto, cuando los lanzadores son los pívots (-19,45%). Por otro lado, los resultados durante la última serie mostraron el efecto negativo significativo sobre la posición de pívot (- 19,30%) y la diferencia parcial en el marcador (-3,33%, para cada punto de diferencia en el marcador) en el porcentaje de acierto en los tiros libres. Las variables independientes edad, experiencia profesional, clasificación en la liga regular, fase de competición, localización, y tiempo restante, no revelaron efectos significativos en los modelos de regresión lineal. Los resultados de la regresión logística binomial revelaron que las variables experiencia profesional entre 13 y 18 años (OR = 4,63), jugar de alero (OR = 23,01), y jugar de base (OR = 10,68) están relacionadas con una baja probabilidad de disminuir el rendimiento durante el último minuto del partido; mientras que ir ganando, aumenta esta probabilidad (OR = 0,06). Además, los resultados de la última serie mostraron una menor disminución del rendimiento del jugador cuando tiene entre 13 y 18 años de experiencia (OR = 4,28), y juega de alero (OR = 8,06) o base (OR = 6,34). Por el contrario, las variables situacionales relacionadas con esa disminución del rendimiento del jugador son las fases eliminatorias (OR = 0,22) e ir ganando (OR = 0,04). Los resultados principales del estudio mostraron que existe una disminución del rendimiento del jugador en su porcentaje de acierto en los tiros libres durante el último minuto y en la última serie de lanzamientos del partido, y que está relacionada significativamente con la edad, experiencia profesional, puesto de juego del jugador, y diferencia parcial en el marcador. Encontrando relación también con la fase de competición, durante la última serie de tiros libres del partido. Esta información supone una valiosa información para el entrenador, y su aplicación en el ámbito competitivo real. En este sentido, la creación de simulaciones en el apartado de aplicaciones prácticas, permite predecir el porcentaje de acierto en los tiros libres de un jugador durante los momentos de mayor presión del partido, en base a su perfil de rendimiento. Lo que puede servir para realizar una toma de decisiones más idónea, con el objetivo de lograr el mejor resultado. Del mismo modo, orienta el tipo de proceso de entrenamiento que se ha de seguir, en relación a los jugadores más tendentes al drop, con el objetivo de minimizar el efecto de la presión sobre su capacidad para rendir adecuadamente en la ejecución de los tiros libres, y lograr de esta manera un rendimiento más homogéneo en todos los jugadores del equipo en esta faceta del juego, durante el momento crítico del final de partido. ABSTRACT. The final minute of a close game in basketball is a critical moment which is subject to many factors that influence its development. Thus, the success rate in free-throws during that period will determine, in many cases, the outcome of the game. Decrease of performance (drop) in this facet of play under pressure conditions, may be related to studied own multiple sports context variables, such as the remaining seconds of possession, the situation in the score (to be winning, drawing, or losing) the location of the match (playing at home or away), the competition phase (regular season or playoffs) or team level (best/worst teams). In addition, the characteristics of the player are very important related to his age and years of experience to face the critical moments, as well as his playing position into team. In this sense, the combination of factors in context and player, allows interact about performance of shooter in the final moments of the game during his free-throw shooting. The aim of this present doctoral thesis was find the most related variables to player´s drop in free throws in the last minute of the game and the last row of free-throws in closed games of basketball. To achieve the objective of the study, 124 closed games (less or equal than 2 points difference) were analyzed in every copetition in ACB league (regular season, playoff and cup) from 2011-2012 to 2014-2015 seasons. To record the variables, the percentage of success of the shooter in regular season, full game, last minute, and last row were analyzed. This way, it is tried to analyze which player and context variables explain the free-throw performance in last minute and last row of the game. On the other hand, it is tried to determine the degree of association between decrease of performance (drop) of the player in the final moments, and studied player variables: playing position, age, and years of professional experience; while considered situational variables considered were: competition phase, location, classification, remaining time, and score-line. For data analysis were performed two statistical models: 1) A multiple linear regression model to determine the effect of the independent variables in the succsess percentage of shooter at the last minute, and in the last row of free-throws in the game; and 2) A binomial logistic regression analysis to analyze the relationship between the probability of a drop (lower performance) and the characteristics of the shooter and situational variables. The results of multiple linear regression model showed significant negative effects on the free-throw percentage during last minute, when shooters are centers (-19.45%). On the other hand, results in the last series showed the significant negative effect on the center position (-19.30%) and score-line (-3.33% for each point difference in the score) in the free-throw percentage. The independent variables age, professional experience, ranking in the regular season, competition phase, location, and remaining time, revealed no significant effects on linear regression models. The results of the binomial logistic regression showed that the variables professional experience between 13 and 18 years (OR = 4.63), playing forward (OR = 23.01) and playing guard (OR = 10.68) are related to reduce the probability to decrease the performance during the last minute of the game. While wining, increases it (OR = 0.06). Furthermore, the results of the last row showed a reduction in performance degradation when player is between 13 and 18 years of experience (OR = 4.28), and playing forward (OR = 8.06) or guard (OR = 6.34). By contrast, the variables related to the decrease in performance of the player are the knockout phases (OR = 0.22) and wining (OR = 0.04). The main results of the study showed that there is a decrease in performance of the player in the percentage of success in free-throws in the last minute and last row of the game, and it is significantly associated with age, professional experience, and player position. Finding relationship with the competition phase, during last row of free-throws of the game too. This information is a valuable information for the coach, for applying in real competitive environment. In this sense, create simulations in the section of practical applications allows to predict the success rate of free-throw of a player during the most pressing moments of the game, based on their performance profile. What can be used to take more appropriate decisions in order to achieve the best result. Similarly, guides the type of training process must be followed in relation to the most favorable players to drop, in order to minimize the effect of pressure on their ability to perform properly in the execution of the free-throws. And to achieve, in this way, a more consistent performance in all team players in this facet of the game, during the critical moment in the final of the game.

Arquifanía: la arquitectura como manifestación

Arquifanía: arquitectura y epifanía. La revelación que se da en el proyectar, en el construir y en el habitar. Desentrañar los protagonistas y el escenario de ese encuentro es el objetivo de esta tesis. Nace de una doble inquietud: la relación entre literatura y arquitectura y la constatación de algo inconmensurable en la arquitectura. A lo largo de los siglos la teoría de la arquitectura ha hecho un gran esfuerzo por racionalizar todos los aspectos de la disciplina. La sistematización del orden y jerarquía de los espacios, el uso de parámetros más rigurosos en los cálculos estructurales, el desarrollo de la técnica y el estudio de los esquemas funcionales han supuesto una fuente inagotable de manifestaciones arquitectónicas. Los paradigmas científicos, incluso, han resultado fundamentales en la creación de nuevos lenguajes. Sin embargo, en este camino de racionalización se han orillado aspectos de difícil cuantificación. Pese a que en la experiencia diaria del arquitecto se constata la presencia de parámetros inconmensurables, la teoría de la arquitectura no ha integrado de forma sistemática este conocimiento. De aquí nace la presente tesis doctoral. En ella se estudia, por analogía con el resto de artes, el concepto de manifestación. Se analiza de qué manera la obra arquitectónica se manifiesta, haciendo uso de su autonomía, y se convierte en la que dirige al arquitecto en todos los tiempos de la existencia de la obra: desde su génesis en el tiempo del proyecto hasta la experiencia de la obra terminada en el tiempo de la historia. Se considera que reflexionar en el concepto de manifestación puede aportar luces sobre la disciplina arquitectónica en un doble momento. Se estudia por una parte la actitud y el proceder del arquitecto ante la génesis del proyecto y por otra su posicionamiento ante la obra ya existente, sometida a su análisis (para la posterior síntesis) generando una espiral de conocimiento siempre abierta y en continuo enriquecimiento. Se analiza la obra de María Zambrano, filósofa, y José Ángel Valente, poeta, en busca de la definición del concepto de manifestación. El momento creativo, la naturaleza de la palabra poética, la actitud del poeta ante su obra, el conocimiento y la revelación de la belleza son temas recurrentes en sus obras y crean un corpus teórico que permite elaborar una teoría poética sólida y abierta, llena de sugerencias que se dirige con precisión al momento de la generación de la forma: su epifanía. A continuación se repasa el concepto de manifestación en la actividad de algunos artistas de la modernidad que, de manera implícita o explícita, han hablado del concepto de epifanía. A menudo de forma intuitiva, los artistas han detectado en su actividad la condición de ser depositarios de un don que se renueva en cada obra ejecutada. Entrelazado con su trabajo y con la disciplina diaria los artistas experimentan un diálogo con la obra, que se revela libre y con voluntad de manifestarse. Enunciado el problema y hecho el repaso por el mundo de la creación artística, nos enfrentamos con el núcleo de la tesis: ¿hasta qué punto y de qué manera el concepto de manifestación es válido en arquitectura? El discurso busca en la actividad de múltiples arquitectos la validez del concepto de manifestación. Se rastrean testimonios, textos, obras y análisis de obras. De esta investigación surge una topografía, un atlas de revelaciones de manifestaciones arquitectónicas: constataciones explícitas, testimonios implícitos o intuiciones. A través de este periplo entre el concepto de manifestación que la razón poética ofrece y la experiencia de muchos arquitectos se intenta responder, aunque sea parcialmente, a las preguntas que surgen al llevar a las últimas consecuencias la arquifanía: ¿En qué momento existe una voluntad de forma? ¿De qué manera encuentra el arquitecto la forma? ¿Cómo se le presenta? ¿Es el arquitecto el verdadero artífice de la obra? ¿O es en cambio un ser a la espera de la aparición? ABSTRACT Archiphany: architecture and epiphany. The revelation taking place in designing, building and living. The objective of the present dissertation is to unravel the protagonists and the scenario of this encounter. It stems from a double interest: the relationship between literature and architecture and the discovery of something incommensurable in architecture. Over the centuries, architectural theory has made a great effort in order to rationalise all aspects of this discipline. Thus, the systematization of the order and hierarchy of space, the use of more rigorous parameters on the structural analysis, the development of technical capacities and the study of functional schemes have been an inexhaustible source of architectural manifestations. Even scientific paradigms have been essential in the creation of new languages. However, aspects of difficult quantification have been relegated in this path of rationalisation. Whereas the presence of immeasurable parameters are encountered in the daily experience of the architect, architectural theory has failed to systematically integrate this knowledge. This is the driving force of this thesis. It explores, by analogy with the other arts, the concept of manifestation. It discusses how the architectural work manifests itself, making use of its autonomy, becoming the one leading the architect during the whole existence of the work: from its genesis in the designing time until the experience of the completed work in the historical time. In the present dissertation, it is assumed that reflecting on the concept of manifestation might shed light on two different moments on the way to project architecture. The architect approach to the project genesis is studied on the one hand, while in the other hand it is considered his positioning in front of the existing work, as well as his analysis (for a following synthesis) that generates an ever-open and ever-enriching spiral of knowledge. In the search for a definition of the concept of manifestation, the work of the philosopher María Zambrano and the poet José Ángel Valente are analysed. The creative moment, the nature of the poetic word, the attitude of the poet in front of his work, the knowledge and the revelation of beauty are persistent issues in their works. They create a theoretical corpus allowing to develop a sound and open poetical theory full of suggestions, accurately directed to the time of the form generation: its epiphany. The concept of manifestation is then reviewed in the activity of some modern artists that talked about the concept of epiphany –either implicitly or explicitly. Often in an intuitive way, artists have come to realize in their activity to be the depositories of a gift that is renewed in each work. Intertwined with their work and with their daily discipline, artists experience a dialogue with the work, which proves to be free and willing to manifest itself. Having stated the problem and having reviewed the world of artistic creation, we are confronted with the core of the thesis: Up to which extent and in which way is the concept of manifestation valid for architecture? Validity and evidence of this concept is sought in the activity of multiple architects. Texts, works, analysis, reviews and testimonies are investigated. From this research, a topography, an atlas of revelations of architectural manifestations arises: explicit findings, implicit witnesses or intuitions. By means of this journey going from the concept of manifestation hinted by the poetic reason to the experience of many architects, the ultimate questions risen by archiphany are meant to be answered (though partially): When does a will of form emerge? How does the architect find the form? How does it appear? Is the architect the true creator of the work? Or is he instead a being waiting for the vision?

Sustentabilidade Empresarial: Um Estudo Comparativo Sobre o Desempenho e Valor Financeiro de Empresas Listadas no Mercado Acionário Brasileiro

Com o crescente aumento da cultura de proteção ambiental, o tema sustentabilidade vem ganhando cada vez mais espaço na vida das pessoas e principalmente na rotina das organizações. Existem algumas correntes teóricas propondo que para existir uma perenidade das organizações, estas devem incorporar em seu plano estratégico, todos os elementos que permitam um equilíbrio nas relações com os stakeholders. Já outras linhas teóricas, defendem que as empresas devem focar todos seus esforços para atender os acionistas (shareholders), maximizando os lucros e agregando valor. Neste contexto, alguns índices de sustentabilidade foram surgindo mundo afora, visando combinar com a já presente visão de resultados financeiros das organizações, também o alcance do bem estar para a sociedade e proteção do meio ambiente. No Brasil, em Novembro de 2005, foi lançando o ISE Índice de Sustentabilidade Empresarial, que foi desenvolvido para uma gama de empresas que apresentam boas práticas de sustentabilidade. Surge, então, o questionamento se a adoção das práticas de sustentabilidade pode de alguma forma, proporcionar melhor desempenho financeiro para as organizações que as adotam, pois de acordo com a teoria dos stakeholders, as instituições que conseguem atender as necessidades das partes interessadas têm seu valor maximizado ao longo do tempo. Com relação a isto, este presente estudo procura averiguar se as empresas que estão listadas no ISE, excluindo as empresas do setor de materiais básicos, utilidade pública, e financeiro, do período de 2006 a 2008, desenvolveram desempenho financeiro superior às outras empresas que não fazem parte do índice no mesmo período. Foram selecionados dados secundários, utilizando a base de dados da Economática, criando dois grupos para comparação, onde um grupo consta de empresas que estão exclusivamente listadas no ISE, consideradas sustentáveis e outro grupo de empresas, formado por empresas listadas no IBOVESPA e que não participaram do ISE. Tomando o Q de Tobin como a variável dependente e índices de Tamanho da Empresa, Distribuição de Dividendos, Debt to Equity, ROA, Crescimento de Vendas, Crescimento dos Investimentos, Diversificação de Mercado, Endividamento como variáveis independentes, foram aplicados testes estatísticos comparando as médias dos índices, seguido do teste t de student, e regressões de dados em painel pooled, fixed e random effects para encontrar a possível correlação entre sustentabilidade e valor da empresa. Os resultados encontrados apontam uma relação entre sustentabilidade empresarial e desempenho financeiro.(AU)

Sustentabilidade Empresarial: Um Estudo Comparativo Sobre o Desempenho e Valor Financeiro de Empresas Listadas no Mercado Acionário Brasileiro

Com o crescente aumento da cultura de proteção ambiental, o tema sustentabilidade vem ganhando cada vez mais espaço na vida das pessoas e principalmente na rotina das organizações. Existem algumas correntes teóricas propondo que para existir uma perenidade das organizações, estas devem incorporar em seu plano estratégico, todos os elementos que permitam um equilíbrio nas relações com os stakeholders. Já outras linhas teóricas, defendem que as empresas devem focar todos seus esforços para atender os acionistas (shareholders), maximizando os lucros e agregando valor. Neste contexto, alguns índices de sustentabilidade foram surgindo mundo afora, visando combinar com a já presente visão de resultados financeiros das organizações, também o alcance do bem estar para a sociedade e proteção do meio ambiente. No Brasil, em Novembro de 2005, foi lançando o ISE Índice de Sustentabilidade Empresarial, que foi desenvolvido para uma gama de empresas que apresentam boas práticas de sustentabilidade. Surge, então, o questionamento se a adoção das práticas de sustentabilidade pode de alguma forma, proporcionar melhor desempenho financeiro para as organizações que as adotam, pois de acordo com a teoria dos stakeholders, as instituições que conseguem atender as necessidades das partes interessadas têm seu valor maximizado ao longo do tempo. Com relação a isto, este presente estudo procura averiguar se as empresas que estão listadas no ISE, excluindo as empresas do setor de materiais básicos, utilidade pública, e financeiro, do período de 2006 a 2008, desenvolveram desempenho financeiro superior às outras empresas que não fazem parte do índice no mesmo período. Foram selecionados dados secundários, utilizando a base de dados da Economática, criando dois grupos para comparação, onde um grupo consta de empresas que estão exclusivamente listadas no ISE, consideradas sustentáveis e outro grupo de empresas, formado por empresas listadas no IBOVESPA e que não participaram do ISE. Tomando o Q de Tobin como a variável dependente e índices de Tamanho da Empresa, Distribuição de Dividendos, Debt to Equity, ROA, Crescimento de Vendas, Crescimento dos Investimentos, Diversificação de Mercado, Endividamento como variáveis independentes, foram aplicados testes estatísticos comparando as médias dos índices, seguido do teste t de student, e regressões de dados em painel pooled, fixed e random effects para encontrar a possível correlação entre sustentabilidade e valor da empresa. Os resultados encontrados apontam uma relação entre sustentabilidade empresarial e desempenho financeiro.(AU)

Use of intrinsic binding energy for catalysis by an RNA enzyme

The contribution of several individual ribozyme⋅substrate base pairs to binding and catalysis has been investigated using hammerhead ribozyme substrates that were truncated at their 3′ or 5′ ends. The base pairs at positions 1.1–2.1 and 15.2–16.2, which flank the conserved core, each contribute 104-fold in the chemical step, without affecting substrate binding. In contrast, base pairs distal to the core contribute to substrate binding but have no effect on the chemical step. These results suggest a “fraying model” in which each ribozyme⋅substrate helix can exist in either an unpaired (“open”) state or a helical (“closed”) state, with the closed state required for catalysis. The base pairs directly adjacent to the conserved core contribute to catalysis by allowing the closed state to form. Once the number of base pairs is sufficient to ensure that the closed helical state predominates, additional residues provide stabilization of the helix, and therefore increase binding, but have no further effect on the chemical step. Remarkably, the >5 kcal/mol free energy contribution to catalysis from each of the internal base pairs is considerably greater than the free energy expected for formation of a base pair. It is suggested that this unusually large energetic contribution arises because free energy that is typically lost in constraining residues within a base pair is expressed in the transition state, where it is used for positioning. This extends the concept of “intrinsic binding energy” from protein to RNA enzymes, suggesting that intrinsic binding energy is a fundamental feature of biological catalysis.

Prime factors

We use Voiculescu’s free probability theory to prove the existence of prime factors, hence answering a longstanding problem in the theory of von Neumann algebras.

Nuclear Ferritin Protects DNA From UV Damage in Corneal Epithelial Cells

Previously, we identified the heavy chain of ferritin as a developmentally regulated nuclear protein of embryonic chicken corneal epithelial cells. The nuclear ferritin is assembled into a supramolecular form indistinguishable from the cytoplasmic form of ferritin found in other cell types and thus most likely has iron-sequestering capabilities. Free iron, via the Fenton reaction, is known to exacerbate UV-induced and other oxidative damage to cellular components, including DNA. Since corneal epithelial cells are constantly exposed to UV light, we hypothesized that the nuclear ferritin might protect the DNA of these cells from free radical damage. To test this possibility, primary cultures of cells from corneal epithelium and stroma, and from skin epithelium and stroma, were UV irradiated, and DNA strand breaks were detected by an in situ 3′-end labeling method. Corneal epithelial cells without nuclear ferritin were also examined. We observed that the corneal epithelial cells with nuclear ferritin had significantly less DNA breakage than other cell types examined. Furthermore, increasing the iron concentration of the culture medium exacerbated the generation of UV-induced DNA strand breaks in corneal and skin fibroblasts, but not in the corneal epithelial cells. Most convincingly, corneal epithelial cells in which the expression of nuclear ferritin was inhibited became much more susceptible to UV-induced DNA damage. Therefore, it seems that corneal epithelial cells have evolved a novel, nuclear ferritin-based mechanism for protecting their DNA against UV damage.

Structure of a cytochrome P450–redox partner electron-transfer complex

The crystal structure of the complex between the heme- and FMN-binding domains of bacterial cytochrome P450BM-3, a prototype for the complex between eukaryotic microsomal P450s and P450 reductase, has been determined at 2.03 Å resolution. The flavodoxin-like flavin domain is positioned at the proximal face of the heme domain with the FMN 4.0 and 18.4 Å from the peptide that precedes the heme-binding loop and the heme iron, respectively. The heme-binding peptide represents the most efficient and coupled through-bond electron pathway to the heme iron. Substantial differences between the FMN-binding domains of P450BM-3 and microsomal P450 reductase, observed around the flavin-binding sites, are responsible for different redox properties of the FMN, which, in turn, control electron flow to the P450.

Multiple benefits of multiple mating in guppies

The rewards of promiscuity for males are undisputed. But why should a female mate promiscuously, particularly when her partners offer no resources other than sperm and increase her chances of succumbing to predation or disease? This question has been hotly debated but at present remains largely unresolved [Jennions, M. D. & Petrie, M. (2000) Biol. Rev. 75, 21–64]. One possibility is that females exploit postcopulatory mechanisms, such as sperm competition, to increase both the quality and quantity of their offspring. In this paper, we use the Trinidadian guppy, a species with a resource-free mating system, to test the hypothesis that females gain multiple benefits from multiple mating. Our results indicate that multiply mated females secure substantive advantages: They have shorter gestation times and larger broods, and they produce offspring with better developed schooling abilities and escape responses than their singly mated counterparts.

Escherichia coli RNA polymerase terminates transcription efficiently at rho-independent terminators on single-stranded DNA templates

Several models have been proposed for the mechanism of transcript termination by Escherichia coli RNA polymerase at rho-independent terminators. Yager and von Hippel (Yager, T. D. & von Hippel, P. H. (1991) Biochemistry 30, 1097–118) postulated that the transcription complex is stabilized by enzyme–nucleic acid interactions and the favorable free energy of a 12-bp RNA–DNA hybrid but is destabilized by the free energy required to maintain an extended transcription bubble. Termination, by their model, is viewed simply as displacement of the RNA transcript from the hybrid helix by reformation of the DNA helix. We have proposed an alternative model where the RNA transcript is stably bound to RNA polymerase primarily through interactions with two single-strand specific RNA-binding sites; termination is triggered by formation of an RNA hairpin that reduces binding of the RNA to one RNA-binding site and, ultimately, leads to its ejection from the complex. To distinguish between these models, we have tested whether E. coli RNA polymerase can terminate transcription at rho-independent terminators on single-stranded DNA. RNA polymerase cannot form a transcription bubble on these templates; thus, the Yager–von Hippel model predicts that intrinsic termination will not occur. We find that transcript elongation on single-stranded DNA templates is hindered somewhat by DNA secondary structure. However, E. coli RNA polymerase efficiently terminates and releases transcripts at several rho-independent terminators on such templates at the same positions as termination occurs on duplex DNAs. Therefore, neither the nontranscribed DNA strand nor the transcription bubble is essential for rho-independent termination by E. coli RNA polymerase.

Randomised controlled trial of the READER method of critical appraisal in general practice

Objective: To evaluate the READER model for critical reading by comparing it with a free appraisal, and to explore what factors influence different components of the model.

Peptide synthesis using unprotected peptides through orthogonal coupling methods.

We describe an approach to the synthesis of peptides from segments bearing no protecting groups through an orthogonal coupling method to capture the acyl segment as a thioester that then undergoes an intramolecular acyl transfer to the amine component with formation of a peptide bond. Two orthogonal coupling methods to give the covalent ester intermediate were achieved by either a thiol-thioester exchange mediated by a trialkylphosphine and an alkylthiol or a thioesterification by C alpha-thiocarboxylic acid reacting with a beta-bromo amino acid. With this approach, unprotected segments ranging from 4 to 37 residues were coupled to aqueous solution to give free peptides up to 54 residues long with high efficiency.

The acceleration and collimation of jets.

I will discuss several issues related to the acceleration, collimation, and propagation of jets from active galactic nuclei. Hydromagnetic stresses provide the best bet for both accelerating relativistic flows and providing a certain amount of initial collimation. However, there are limits to how much "self-collimation" can be achieved without the help of an external pressurized medium. Moreover, existing models, which postulate highly organized poloidal flux near the base of the flow, are probably unrealistic. Instead, a large fraction of the magnetic energy may reside in highly disorganized "chaotic" fields. Such a field can also accelerate the flow to relativistic speeds, in some cases with greater efficiency than highly organized fields, but at the expense of self-collimation. The observational interpretation of jet physics is still hampered by a dearth of unambiguous diagnostics. Propagating disturbances in flows, such as the oblique shocks that may constitute the kiloparsec-scale "knots" in the M87 jet, may provide a wide range of untapped diagnostics for jet properties.

Cerebrovascular alterations in mice lacking neuronal nitric oxide synthase gene expression.

Nitric oxide (NO) is known to mediate increases in regional cerebral blood flow elicited by CO2 inhalation. In mice with deletion of the gene for neuronal NO synthase (NOS), CO2 inhalation augments cerebral blood flow to the same extent as in wild-type mice. However, unlike wild-type mice, the increased flow in mutants is not blocked by the NOS inhibition, N omega-nitro-L-arginine, and CO2 exposure fails to increase brain levels of cGMP. Topical acetylcholine elicits vasodilation in the mutants which is blocked by N omega-nitro-L-arginine, indicating normal functioning of endothelial NOS. Moreover, immunohistochemical staining for endothelial NOS is normal in the mutants. Thus, following loss of neuronal NOS, the cerebral circulatory response is maintained by a compensatory system not involving NO.

DNA-strand exchange promoted by RecA protein in the absence of ATP: implications for the mechanism of energy transduction in protein-promoted nucleic acid transactions.

DNA-strand exchange promoted by Escherichia coli RecA protein normally requires the presence of ATP and is accompanied by ATP hydrolysis, thereby implying a need for ATP hydrolysis. Previously, ATP hydrolysis was shown not to be required; here we demonstrate furthermore that a nucleoside triphosphate cofactor is not required for DNA-strand exchange. A gratuitous allosteric effector consisting of the noncovalent complex of ADP and aluminum fluoride, ADP.AIF4-, can both induce the high-affinity DNA-binding state of RecA protein and support the homologous pairing and exchange of up to 800-900 bp of DNA. These results demonstrate that induction of the functionally active, high-affinity DNA-binding state of RecA protein is needed for RecA protein-promoted DNA-strand exchange and that there is no requirement for a high-energy nucleotide cofactor for the exchange of DNA strands. Consequently, the free energy needed to activate the DNA substrates for DNA-strand exchange is not derived from ATP hydrolysis. Instead, the needed free energy is derived from ligand binding and is transduced to the DNA via the associated ligand-induced structural transitions of the RecA protein-DNA complex; ATP hydrolysis simply destroys the effector ligand. This concept has general applicability to the mechanism of energy transduction by proteins.