DEVELOPMENT of MICROSATELLITE MARKERS FOR PIMENTA PSEUDOCARYOPHYLLUS (MYRTACEAE), A WILD SOUTH AMERICAN SPECIES

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparison of clinical features and drug therapies among European and Latin American patients with juvenile dermatomyositis

ObjectiveTo compare the demographic features, presenting manifestations, diagnostic investigations, disease course, and drug therapies of children with juvenile dermatomyositis (JDM) followed in Europe and Latin America.MethodsPatients were inception cohorts seen between 1980 and 2004 in 27 paediatric rheumatology centres. The following information was collected through the review of patient charts: sex; age at disease onset; date of disease onset and diagnosis; onset type; presenting clinical features; diagnostic investigations; course type; and medications received during disease course.ResultsFour hundred and ninety patients (65.5% females, mean onset age 7.0 years, mean disease duration 7.7 years) were included. Disease presentation was acute or insidious in 57.1% and 42.9% of the patients, respectively. The course type was monophasic in 41.3% of patients and chronic polycyclic or continuous in 58.6% of patients. The more common presenting manifestations were muscle weakness (84.9%), Gottron's papules (72.9%), heliotrope rash (62%), and malar rash (56.7%). Overall, the demographic and clinical features of the 2 continental cohorts were comparable. European patients received more frequently high-dose intravenous methylprednisolone, cyclosporine, cyclophosphamide, and azathioprine, while methotrexate and antimalarials medications were used more commonly by Latin American physicians.ConclusionThe demographic and clinical characteristics of JDM are similar in European and Latin American patients. We found, however, several differences in the use of medications between European and Latin American paediatric rheumatologists.

Self-fertility and polyembryony in South American yellow trumpet trees (Handroanthus chrysotrichus and H. ochraceus, Bignoniaceae): a histological study of postpollination events

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The reproductive biology of Cybistax antisyphilitica (Bignoniaceae), a characteristic tree of the South American savannah-like "Cerrado" vegetation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Horizontal distribution of megalopae of Brachyura (Crustacea, Decapoda) in a South American tropical estuary

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A GEP-ISFG collaborative study on the optimization of an X-STR decaplex: data on 15 Iberian and Latin American populations

In a collaborative work carried out by the Spanish and Portuguese ISFG Working Group (GEP-ISFG), a polymerase chain reaction multiplex was optimized in order to type ten X-chromosome short tandem repeats (STRs) in a single reaction, including: DXS8378, DXS9902, DXS7132, DXS9898, DXS6809, DXS6789, DXS7133, GATA172D05, GATA31E08, and DXS7423. Using this X-decaplex, each 17 of the participating laboratories typed a population sample of approximately 200 unrelated individuals (100 males and 100 females). In this work, we report the allele frequencies for the ten X-STRs in 15 samples from Argentina (Buenos Aires, CA(3)rdoba, Rio Negro, Entre Rios, and Misiones), Brazil (SA o pound Paulo, Rio de Janeiro, Parana, and Mato Grosso do Sul), Colombia (Antioquia), Costa Rica, Portugal (Northern and Central regions), and Spain (Galicia and Cantabria). Gene diversities were calculated for the ten markers in each population and all values were above 56%. The average diversity per locus varied between 66%, for DXS7133, and 82%, for DXS6809. For this set of STRs, a high discrimination power was obtained in all populations, both in males (a parts per thousand yen1 in 5 A- 10(5)) and females (a parts per thousand yen1 in 3 A- 10(9)), as well as high mean exclusion chance in father/daughter duos (a parts per thousand yen99.953%) and in father/mother/daughter trios (a parts per thousand yen99.999%). Genetic distance analysis showed no significant differences between northern and central Portugal or between the two Spanish samples from Galicia and Cantabria. Inside Brazil, significant differences were found between Rio de Janeiro and the other three populations, as well as between SA o pound Paulo and Parana. For the five Argentinean samples, significant distances were only observed when comparing Misiones with Entre Rios and with Rio Negro, the only two samples that do not differ significantly from Costa Rica. Antioquia differed from all other samples, except the one from Rio Negro.

Morphological and anatomical observations about Mariliasuchus amarali and Notosuchus terrestris (Mesoeucrocodylia) and their relationships with other South American notosuchians.

The phylogenetic relationship of the notosuchians Mariliasuchus amarali (Campanian; Bauru Group) and Notosuchus terrestris (Santonian; Neuquen Group) is revised. Morpho-anatomical evaluation of Mariliasuchus in the current bibliography indicate close relationship with Notosuchus, while cladistic analysis either related Mariliasuchus to Candidodon itapecuruense (Albian/eo-Cenomanian; Sao Luis-Grajau Basin), as part of the phylotaxon Candidodontidae, or to Comahuesuchus brachybuccalis (Santonian; Neuquen Group). Comparative study of specimens shows similarities on the palate, choanae, dentition, retroarticular process, and other structures from Mariliasuchus and Notosuchus supporting the original classification as a Notosuchidae. Preliminary phylogenetic analysis sets these taxa as sister-groups. Reevaluation of a previously published phylogenetic analysis from other authors provides further support for the Mariliasuchus + Notosuchus clade. The current work indicates that Mariliasuchus is a Notosuchidae, refuting its allocation as a Candidodontidae. The influence of character construction and the definition of Notosuchia are discussed.

Feeding preference of the South American endemic anomuran Aegla platensis (Decapoda, Anomura, Aeglidae)

In order to determine the feeding preference of Aegla platensis in streams and the importance of microorganisms in its detritivore diet, we carried out two experiments designed to evaluate the food preferences of A. platensis (1) among leaves with different levels of microbial colonization and (2) among insect larvae (Chironomidae, Simuliidae, Hydropsychidae) and microbially conditioned leaves. A. platensis preferred animal over plant food items; when only leaves were offered, this aeglid preferred the leaves with higher levels of microorganism conditioning.

On the cardiac control in the South American lungfish, Lepidosiren paradoxa

1. 1. The mechanisms behind cardiac control were investigated in the South American lungfish, Lepidosiren paradoxa, using fish with chronically implanted cannulae and electromagnetic flow probes. In addition, a preliminary study was made of the cardiovascular events associated with air breathing. 2. 2. The study suggests that the heart of Lepidosiren is controlled by cholinergic vagal fibres which, in some animals, exert a tonic influence in the resting fish. Cyclic changes in heart rate in association with air breaths is due to modulation of this cholinergic tonus. 3. 3. In addition to the variable cholinergic tonus, there appears to be a relatively stable adrenergic tonus on the heart, which causes an elevated heart rate. The adrenergic tonus is likely to be due to local release of catecholamines from endogenous chromaffin cells within the atrium. 4. 4. Preliminary results suggest that pulmonary arterial flow increases by about 50% immediately following an air breath. The mechanism behind this increase probably involves both an elevation of the heart rate and a redistribution of blood flow into the pulmonary circuit. © 1989.

Cytotaxonomic considerations with the description of two new NOR locations for South American toads, genus Bufo (Anura: Bufonidae)

Cytogenetic studies were made on Brazilian Bufo: B. marinus, B. paracnemis, B. ictericus, B. rufus, B. arenarum, B. crucifer, Bufo granulosus, B. pygmaeus and B. margaritifer (= B. typhonius). All these species had a typical karyotype of 2n = 22. Species from the marinus and crucifer groups had NORs on Chromosome 7, species from the granulosus group had NORs on Chromosome 5, and B. margaritifer had NORs on Chromosome 10. The last two locations of NORs are described for the first time for the genus Bufo in South America.

Endocytic activity in the thrombocytes of the turtle Phrynopys hilarii (freshwater South American species)

The phagocytic process in cells depends on lysosomal enzymes, high-energy metabolism and cellular recognition. In this paper, we investigated the presence of energy and recognition factors in thrombocytes of turtle Phrynopys hilarii (a freshwater South American species). Turtle thrombocytes (P. hilarii) present glycogen - possibly β particles - dispersed in their cytoplasm and glycoproteins in the cell surface, as well as a large number of enzymes involved in the endocytic process (Pellizzon, 1996). The activity of these enzymes depends on high-energy metabolism and on cellular recognition provided by specific glycoconjugates (Alberts et al., 1994). This metabolic characterization is demonstrated by the large amount of glycogen particles observed in the cytoplasm by Thiéry's method. Glycogen labeling was also observed when concanavalin A-peroxidase was used as a marker for thrombocytes and for endocyted charcoal particles. Our results show that these cells have phagocytic ability, suggesting that their function in blood circulation is not limited to aggregation but may also involve a great potential for phagocytosis.

Indoor radon measurements in six Latin American countries

Most of the natural radiation dose to man comes from radon gas and its progeny. Several countries have established national institutions and national programs in charge of the study of radon and its connection with lung cancer risk and public health. In this paper an indoor radon measurements in Latin American countries is presented. The participants in this work were from Argentina, Brazil, Ecuador, Mexico, Peru and Venezuela. Many different techniques are used in this common effort, and the indoor radon levels in specific locations in each of the participant countries are presented.

Development of a low-cost mobile mapping system: A South American experience

This paper presents the prototype of a low-cost terrestrial mobile mapping system (MMS) composed of a van, two digital video cameras, two GPS receivers, a notebook computer, and a sound frame synchronisation system. The imaging sensors are mounted as a stereo video camera on top of the vehicle together with the GPS antennae. The GPS receivers and the notebook computer are configured to record data referred to the vehicle position at a planned time interval. This position is subsequently transferred to the road images. This set of equipment and methods provide the opportunity to merge distinct techniques to make topographic maps and also to build georeferenced road image databases. Both vector maps and raster image databases, when integrated appropriately, can give spatial researchers and engineers a new technique whose application may realise better planning and analysis related to the road environment. The experimental results proved that the MMS developed at the São Paulo State University is an effective approach to inspecting road pavements, to map road marks and traffic signs, electric power poles, telephone booths, drain pipes, and many other applications important to people's safety and welfare. A small number of wad images have already been captured by the prototype as a consequence of its application in distinct projects. An efficient organisation of those images and the prompt access to them justify the need for building a georeferenced image database. By expanding it, both at the hardware and software levels, it is possible for engineers to analyse the entire road environment on their office computers.

Mononeuropathy of the suprascapular nerve: Study of 3 South American cases

In this work 3 new cases of suprascapular nerve mononeuropathy are described. ENMG diagnosis criteria were: a) normal sensory conduction studies of the ipsolateral ulnar, median and radial nerves; b) bilateral suprascapular nerve latencies with bilateral compound muscle action potential, obtained from the infraspinatus muscle with symmetrical techniques; and c) abnormal neurogenic infraspinatus muscle electromyographic findings, coexisting with normal electromyographical data of the ipsolateral deltoideus and supraspinatus muscles. These 3 cases of suprascapular mononeurpathy were found in 6,080 ENMG exams from our University Hospital. For us this mononeuropathy is rare with a 0.05% occurrence.