Preliminary observations in the design and operation of a three panel, double trawl net

Fishing operations were conducted off Kakinada using a 3 panel double trawl net with twin cod-ends to study the utility of the net in catching both bottom and off bottom fishes. The observations indicate that the net is effective in simultaneous catching of bottom as well as off-bottom fishes and separating them while in operation. The design details of the net and the particulars of the fishing operations conducted are presented.

Double-rig shrimp trawling, its rigging, comparative efficiency and economics

A radical and revolutionary change from the hitherto employed shrimp trawling is reported for better productivity and economy in deep sea trawling with medium and large vessels. Studies carried out off Veraval with a 13.7 m. four seam shrimp trawl as conventional single-rig and two 6.8 m four seam shrimp trawls as double-rig from a 15.2 m fishing vessel showed an increase of 98% shrimp catch by double-rig over single-rig at 86.3 % of the power utilised by the latter. It has also been worked out, theoretically, how better this available power can be utilised for further improvement of catch. An increase of 15% head rope length in case of double-rig over the optimum single-rig gear of any vessel is recommended.

Effects of exogenous double-stranded RNA on the basonuclin gene expression in mouse oocytes

In plants and less-advanced animal species, such as C.elegans, introduction of exogenous double-stranded RNA (dsRNA) into cells would trigger degradation of the mRNA with homologous sequence and interfere with the endogenous gene expression. It might represent an ancient anti-virus response which could prevent the mutation in the genome that was caused by virus infection or mobile DNA elements insertion. This phenomenon was named RNA interference, or RNAi. In this study, RNAi was used to investigate the function of basonuclin gene during oogenesis. Microinjection of dsRNA directed towards basonuclin into mouse germinal-vesicle-intact (GV) oocytes brought down the abundance of the cognate mRNA effectively in a time- and concentration-dependent manner. This reduction effect was sequence-specific and showed no negative effect on other non-homologous gene expression in oocytes, which indicated that dsRNA can recognize and cause the degradation of the transcriptional products of endogenous basonuclin gene in a sequence-specific manner. Immunofluorescence results showed that RNAi could reduce the concentration of basonuclin protein to some extent, but the effect was less efficient than the dsRNA targeting towards tPA and cMos which was also expressed in oocytes. This result might be due to the long half life of basonuclin protein in oocytes and the short reaction time which was posed by the limited life span of GV oocytes cultured in vitro. In summary, dsRNA could inhibit the expression of the cognate gene in oocytes at both mRNA and protein levels. The effect was similar to Knock-out technique which was based on homologous recombination. Furthermore, hairpin-style dsRNA targeting basonuclin gene could be produced by transcription from a recombinant plasmid and worked efficiently to deplete the cognate mRNA in oocytes. This finding offered a new way to study the function of basonuclin in the early stage of oogenesis by infection of primordial oocytes with the plasmid expressing hairpin-style basonuclin dsRNA.

The successful double cryopreservation of rabbit (Oryctolagus cuniculus) semen in large volume using the directional freezing technique with reduced concentration of cryoprotectant

Using directional freezing, Our objective was to cryopreserve rabbit semen and achieve fertility that was equal or higher than that achieved with conventional freezing. The working hypothesis was that controlling the ice-front propagation would allow redu

Prefrontal white matter abnormalities in young adult with major depressive disorder: A diffusion tensor imaging study

Prefrontal impairments have been hypothesized to be most strongly associated with the cognitive and emotional dysfunction in depression. Recently, white matter microstructural abnormalities in prefrontal lobe have been reported in elderly patients with ma