Long-term results after operations for active infective endocarditis in native and prosthetic valves

The objective of this study was to evaluate the midterm results of patients who underwent operations for active infective endocarditis.

Distinct reactivities of interleukin-4-specific antibodies with recombinant and native canine interleukin-4 in various assays

Interleukin 4 (IL-4) plays a central role in immune responses to parasites and allergens. IL-4 drives the differentiation of naive T cells into Th2 cells and regulates immunoglobulin class switching to IgE.Little is known about the role of IL-4 in canine allergies and parasite infections. Most of the information derives from measurement of IL-4 mRNA expression in dog tissues, but detection of IL-4 protein has been difficult so far, probably due to low sensitivity of available methods. Antibodies (Ab) specific for canine IL-4 are available from various sources, but these Ab have been produced against recombinant Escherichia coli-expressed canine IL-4 and there is only limited information on their reactivities with native canine IL-4. Therefore, in the present study, we tested six available canine IL-4-specific Ab for their reactivities with recombinant canine IL-4 expressed in E. coli (rec.IL-4) or in mammalian cells (mam.IL-4), and with supernatants from stimulated canine peripheral blood mononuclear cells (PBMCs) using several detection methods, including Western blotting, ELISA, cytokine bead assay, and intracellular IL-4 staining. Additionally, we tested a bovine IL-4-specific antibody that has been previously shown to cross-react with canine IL-4. All tested Ab except anti-bovine IL-4 reacted with rec.IL-4, and most of them reacted with mam.IL-4. However, only the cytokine bead assay was sensitive enough to allow the detection of IL-4 in supernatants of canine PBMCs.

A Comparative Study of English Word Order Acquisition by Elementary and Secondary School Pupils - Native Speakers of Serbian and Hungarian

Kitic investigated the phenomenon of English word order acquisition by Serbian and Hungarian speakers, examining both the theoretical and empirical aspects of this phenomenon. She began by looking at language learning and language acquisition, viewing word order acquisition in the context of relevant linguistic and psycholinguistic knowledge. The main hypothesis of her empirical investigation was that the majority of word order mistakes in the language production of Serbian and Hungarian speakers of English is due to mother tongue interference. Three supporting hypotheses were introduced to specify the phenomenon of interference in its correlation to (1) language proficiency, (2) sentence patterns, and (3) optional adverbials. The conclusions were based on error analysis of 9280 sentences of 464 elementary and high school learners. The results showed that a learner's level of proficiency seems to be a relevant factor in mother tongue interference as this decreases with increased proficiency. Word order errors are however fossilised at the highest levels. The causes of interference errors, which increase with the number of sentence elements, are either absent sentence patterns or similar ones. In the case of adverbials, word order errors have two forms: interrelation (with canonical elements) and mixed adverbials.