958 resultados para Azul de toluidina O


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Pós-graduação em Odontologia Restauradora - ICT

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Fundação de Amparo à Pesquisa do Estado deo Paulo (FAPESP)

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The purpose of this study was to evaluate the apical sealing ability and the marginal adaptation of five dental materials used in retrofillings or applied to the bevelled root surface. One hundred and forty extracted single rooted human teeth were used, divided into seven groups of twenty each. ln the first, second, third and fourth groups, the teeth were apicoectomized, submitted to cavity preparations and retrofilled with one of the following materiais: zinc free silver amalgam, a dentin bonding system plus composite resin, a glass ionomer cement ora compomer. In the fifth, sixth and seventh groups, the teeth were apicoectomized and capped on the bevelled root surface with one of the following materiais: a dentin bonding system plus composite resin, a glass ionomer cement or a compomer. Two specimens of each experimental group were evaluated for the marginal adaptation using scanning electron microscopy. The remaining 126 specimens were immersed in 2% methylene blue dye, stored for one week at 37ºC and the infiltration was evaluated with a stereomicroscope. The results showed that the glass ionomer cement presented the lowest values of marginal infiltration when used as retrofilling material, with a significant statistical difference when compared with the others tested materials. When used as apical capping, the glass ionomer cement and the compomer were equivalent and significantly better than the dentin bonding system plus composite resin. Using scanning electron microscopy, all the materials showed some slight adjustment problem. ln the retrofilling, the smallest marginal gaps were observed with the compomer and the dentin bonding system plus composite resin, while the largest were observed with the glass ionomer cement and zinc free silver amalgam. ln the apical capping, the smallest marginal gaps were observed with the compomer and the dentin bonding system plus composite resin and ...

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Objetivo: o objetivo desse estudo in vitro foi avaliar a infiltração marginal coronária após o preparo para retentor em dentes obturados com três diferentes cimentos, de acordo com o período entre a obturação do canal radicular e o preparo para retentor. Métodos: Noventa dentes humanos recentemente extraídos foram limpos e instrumentados e, então, obturados com Sealapex, Endométhasone ou TopSeal. Brocas Gates-Glidden foram utilizadas para o preparo imediato de 10 dentes de cada cimento até manter 5mm de obturação remanescente. Sessenta raízes obturadas foram incubadas a 37°C em meio úmido por 30 e 60 dias para serem, depois, preparadas para os retentores como descrito anteriormente. A superfície externa de cada raiz foi coberta com Araldite. Os espécimes foram imersos em corante azul de metileno a 2% sob vácuo por 24h, para então poderem ser analisados. A infiltração foi mensurada pelo software Sigma Scan da parte superior da obturação à porção apical alcançada pelo corante. Resultado: o Sealapex e o TopSeal apresentaram menor infiltração após o preparo para retentor do que o Endométhasone. O preparo imediato para o retentor apresentou menor infiltração do que o preparo para o retentor após 30 e 60 dias da obturação do canal radicular.

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Cryptococcosis is an opportunistic fungal infection caused by Cryptococcus yeasts, especially C. neoformans and Cryptococcus gattii. The fungus is found in substrates of animal and vegetable origin, and infection occurs through inhalation and seedlings present in the environment. The present study aimed to investigate the existence of microfocus Cryptococcus sp. from the environmental samples of Araçatuba city, São Paulo, featuring new niches, by decoupling the direct relationship between fungus and host in order to minimize the risk of contamination of man and animals, understanding the ecoepidemiology of Cryptococcus. Fifty samples from hollows and tree trunks were harvested (Cassia sp., Ficus sp., Caesalpinea peltophorides) from ten representatives in the urban perimeter. The samples were immediately sent to the Laboratory of Bacteriology and Mycology, Faculty of Veterinary Medicine Araçatuba - Unesp where they were processed and plated on Petri dishes containing agar seed Niger and Sabouraud dextrose agar with chloramphenicol, incubated at 30ºC for a period of no less than 5 days. Afterwards they were subimitted to biochemical tests: urease production, thermotolerance at 37°C and quimiotipagem in CGB agar (L- Canavanine-Glycine-Bromothymol blue). The results showed that 17 (34%) cultures were positive for Cryptococcus, 9 (18%) for Cryptococcus gattii and 8 (16%) for Cryptococcus neoformans. Other yeast correlated as Rhodotorula sp. and Candida sp. were isolated. We conclude that the infectious propagules of Cryptococcus are dispersed in nature and constitute an environmental microfocus, not necessarily being bound to a single host.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Fundação de Amparo à Pesquisa do Estado deo Paulo (FAPESP)

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Pós-graduação em Agronomia (Ciência do Solo) - FCAV

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Agronomia (Produção Vegetal) - FCAV