Genetic diversity of Bartail flathead (Platycephalus indicus (Linnaeus,1758)) using AFLP markers in Persian Gulf

Platycephalus indicus is a large benthic fish that inhabits temperate and tropical coastal waters of the Indo-West Pacific and found on sand or mud bottom in vary shallow area of estuary and near shore to depth of 25m. This species is dominant species of platycephalidae family, in Khuzestan, Bushehr and Hormozgan provinces and mainly is captured by bottom trawl, gillnet and moshta in Hormozgan. This study was designed to evaluate population variation and differentiation of bartail flathead (Platycephalus indicus (Linnaeus, 1785))in the Iranian waters of Persian Gulf using the morphometric and meristic characters and by AFLP marker. . A total 180 fish specimens were collected by gill net from six station(khor mosa, bahrekan, shif, motaf, charak and bandar abbas) that was 30 individual related to every station in Iranian shores of Persian Gulf . 28 morphometric factors and 11meristic specialties were measured and morphometric factors was standardized with Beacham formula. Univariate analysis of variance (One-way ANOVA) revealed significant differences with varying degrees between the means for 21 standardized morphometric measurements and 6 meristic counts that showed high significant differences between the six stations sampling. Discriminate function analysis (DFA) or the overall random assignment of individuals into their original groups was for morphometric and meristic characters was 47.9% and 53.9% respectively. The data were subjected to a principle component analysis (PCA) which grouped in eight and four factors for morphometric and meristic charactersrespectively.. Genetic diversity of six populations of bartail flathead (Platycephalus indicus) was investigated using amplified fragment length polymorphism (AFLP). A total of 118 reproducible bands amplified with ten AFLP primer combinations were obtained from 42 fishes that were collected from six different locations in the northern of Persian Gulf. The percentage of polymorphic bands was 57.06%. Average of Nei’s genetic diversity was 0.200±0.008, and Average of Shannon’s index was 0.300±0.011. The results of AMOVA analysis indicated that 66% of the genetic variation contained within populations and 34% occurred among populations and gene flow was 0.6454.The estimated level of population differentiation asmeasured by average Fst value across all loci was 0.327. Plotting discriminant functions 1 and 2 and UPGMA dendrograms based on Euclidian distance and genetic distance also showed at least five separate populations of bartail flathead in the northern Persian Gulf.

A comparative ecological study on internationally important wetlands of Khoor-e-Kolahy and Tiab

Ecological study on Kolahy and 'flab estuaries were carried out during 1996-1997. Water and sediment sampling was done seasonally. In order to study the physical and chemical characteristic of the water, various animal fauna from different material niches including planktons and fish from water, benthousi from the sediment , and surface living animal such as Arthropod (Crabs) and Waterbirds were identified. I he result shoved, that water salinity of both estuaries was the same as the general salinity existing its the Persian Gulf water, and its variation is same IN those waters Minimum water salinity in both estuaries are seen during Bahairdan or early winter mooth(Jaa.), which is about 31ppt and Maximum is during .rirldlun.) or summer mouth at about 19 ppt. Dissolved otygen and pH are slime as the general Persian Gulf waters., Dissolve Oxygen being directly associated to temperature and its fluctuation is between 7- Sing/I and pH between 7.5-LS. The animals of both estuaries are almost similar having a sal factory species diversity . The birds of the region are often seasonal migrants , the maximum population of which occurs in winter season and the minimum during summer month. Nam coverage is richer in Tiab than Kolahy estuary, where in Kolahy a signal Mangrove tree is in exigence. 'Ile total coverage of Mangrove forest in Tiab is estimated about -29 hec.Both estuaries are included with in the international Ramsar Convention sites in 1971. Due to national importance of these estuaries inproviding refugee for various birds species and also hinting grounds and access traffic for local fishing vehicles, Actiog as an important access for various inhabitancy living with these area of the Persian Gulf. Due to importance or prawn aquacultur for the economical well being of local inhabitance, these areas provide a suitable grandees for prawn production. I-test statistics show, there are no significant difference among various invertebrate and vertebrate animals. In over all out look 21) phytoplankton genera, 21) zeoplankton genera 17 miafauna and 32 roaerofauna genera , 11 different species Carcineacearions and 119 species of walerbirds were identified with in the Tiab and Kolahy estuariesregioos. The X statistic show that the animal density is directly associated to season. Where density of miofauna and maerofauna in both estuaries are in maiticrourn during summer and the minimums existing during the winter season. In addition the bentic invertebrate population density in closlly association to birds population density since , the later, feeds on the former animals. Where the increasing in bird population density during the winter season, the bentic invertebrate animal population density decreases. The over all trend of animal population density in winter tend to increased towards the summer seasons, which this is due to climatic conditions of the region. The bird population on the contrary to other animals of both estuaries tend to increase from summer towards the winter seasons and which this bird population density is in maximum in winter with in the region.

Spring Diatom Blooming Phases in a Representative Eutrophic Bay of the Three-Gorges Reservoir, China

We investigated dynamics of the phytoplankton community and abiotic factors in Xiangxi Bay of the Three-Gorge Reservoir, China, by daily sampling, a specific site during a spring algal bloom (February 23-April 28, 2005). Among the 76 taxa observed, Asterionella formosa and Cyclotella spp. were the dominants, accounting for 47.2% and 29.9% of the total abundance, respectively. We determined the five distinct developing phases of the bloom by analyzing the dissimilarity of physicochemical parameters. Simultaneously, six phytoplankton community groups were distinguished by TWINSPAN classifications. The pattern for algal community succession was similar to that for the bloom phase shift, and the structural complexity of communities significantly decreased over time. Water temperature and silicate were the main factors that related to the development of the bloom and the shifts of the phytoplankton community.

How polluted is the Yangtze River? Water quality downstream from the Three Gorges Dam

The concentrations of major anions and cations, nitrogen and phosphorus, dissolved and particulate trace elements, and organic pollutants were determined for the middle and lower reaches of the Yangtze River (Changjiang) from below the Three Gorges Dam (TGD) to the mouth at Shanghai in November 2006. The concentration of dissolved inorganic phosphate (DIP) was constant at a low level of 6-8 mu gP/L, but the concentration of nitrate (NO3-) approximately doubled downstream and was closely correlated with K+. This translated to a daily load of well over 1000 It of dissolved inorganic nitrogen (DIN) at Datong. The average concentrations of dissolved Pb (0.078 +/- 0.023 mu g/L), Cd (0.024 +/- 0.009 mu g/L), Cr(0.57 +/- 0.09 mu g/L), Cu (1.9 +/- 0.7 mu g/L), and Ni (0.50 +/- 0.49 mu g/L) were comparable with those in other major world rivers, while As (3.3 +/- 1.3 mu g/L) and Zn (1.5 +/- 0.6 mu g/L) were higher by factors of 5.5 and 2.5, respectively. The trace element contents of suspended particles of As (31 +/- 28 mu g/g), Pb (83 +/- 34 mu g/g), and Ni (52 +/- 16 mu g/g) were close to maximum concentrations recommended for rivers by the European Community (EC). The average concentrations of Cd (2.6 +/- 1.6 mu g/g), Cr (185 +/- 102 mu g/g), Cu (115 +/- 106 mu g/g), and Zn (500 +/- 300 mu g/g) exceeded the EC standards by a factor of two, and Hg (4.4 +/- 4.7 mu g/g) by a factor of 4 to 5. Locally occurring peak concentrations exceed these values up to fourfold, among them the notorious elements As, Hg, and Tl. All dissolved and particulate trace element concentrations were higher than estimates made twenty years ago [Zhang, J., Geochemistry of trace metals from Chinese river/estuary systems: an overview. Estuar Coast Shelf Sci 1995; 41: 631-658.]. The enormous loads of anthropogenic pollutants disposed to the river were diluted by the large water discharge of the Yangtze even during the lowest flow resulting in the relatively low concentration levels of trace elements and organic pollutants observed. We estimated loads of e.g. As, Pb and Ni to the East China Sea to be about 4600 kg As d(-1), 3000 kg Pb d(-1), and 2000 kg Ni d(-1). About 6000 t d(-1) of dissolved organic carbon (DOC) was delivered into the sea at the time of our cruise. We tested for 236 organic pollutants, and only the most infamous were found to be barely above detection limits. We estimated that the load of chlorinated compounds, aromatic hydrocarbons, phenols, and PAHs were between 500 and 3500 kg d(-1). We also detected eight herbicides entering the estuary with loads of 5-350 kg d(-1). The pollutant load, even when at low concentrations, are considerable and pose an increasing threat to the health of the East China Sea ecosystem. (c) 2008 Elsevier B.V. All rights reserved.

Gonad development of an anadromous fish Coilia ectenes (Engraulidae) in lower reach of Yangtze River, China

Anadromous Coilia ectenes was sampled from the Yangtze estuary at Chongming and two of the primary upstream spawning grounds at Jingjiang and Anqing in April, May, June and August 2006. Gonad development was analyzed for females. In April, fish were collected in the estuary and at Jingjiang, but not at Anqing. No female was mature (gonad at stages IV or V) at either location. In May, 45% of the females were mature in the estuary, 9% at Jingjiang and 5% at Anqing. In June, 86% were mature in the estuary, 83% at Jingjiang and 7% at Anqing. In August, C. ectenes was absent at Jingjiang. No female was mature in the estuary, and all females were mature at Anqing. Absolute fecundity (AF) increased significantly with standard length (SL) by a power function AF = 2.27 x 10(-6) x SL2.67 (r(2) = 0.57, n = 48, P < 0.05). Mature females in the estuary were smaller than those at Jingjiang and Anqing. Conservation of spawners in the upstream spawning grounds is important because they have a size-related fecundity advantage over the smaller spawners in the estuary.

Daily dynamics of nutrients and chlorophyll a during a spring phytoplankton bloom in Xiangxi Bay of the Three Gorges Reservoir

We studied the daily dynamics of nutrients (total phosphorus [TP], total nitrogen [TN], and dissolved silicate [SiO2]) and chlorophyll a (chl a) during a spring bloom in Xiangxi Bay of the Three Gorges Reservoir in year 2005. According to the daily dynamics of chl a, the bloom occurred in two stages (23 February-25 March and 26 March-28 April). The concentration of SiO2 decreased at different layers of the water column with the development of the bloom. However, the decrease of SiO2 in the layers with high concentration of chl a was more dramatic than in the layers with low concentration of chl a. The concentration of TP was lowest value a few days after the peak of chl a during the first bloom period, and the lowest value of TN was found a few days after the peak of chl a during the second bloom period. Correlative analyses indicated that SiO2 and TP were limiting factors in the first bloom period, and SiO2 and TN were limiting factors in the second bloom period.

Monitoring organochlorine pesticides and polychlorinated biphenyls in Baiyangdian Lake using microbial communities

We used polyurethane foam units (PFUs) to collect persistent organic pollutants (POPs) from four sites in Baiyangdian Lake in July 2003. Following extraction from the PFUs, relative concentrations of seven organochlorine pesticides (OCPs) and ten polychlorinated biphenyls (PCBs) were determined by gas chromatography. OCPs and PCBs were detected in the microbial communities from all the four sampling stations. In terms of the total concentration of POPs (OCPs+ PCBs), two river estuary stations had more POP (18.45 mu g/L and 9.77 mu g/L) than the two mid-lake stations (4.75 mu g/L and 5.21 mu g/L), indicating that Baiyangdian Lake was significantly impacted by inflow from the Fu River and Baigou River.

连山湾河口典型重金属的吸附解吸规律及机制

锦州湾海域自上世纪30年代开始接纳大量的含重金属的废水、废气及废渣的排放，是我国重金属污染最严重的海域之一。湾内五里河河口区域污染尤其严重，生态环境遭到严重破坏，至上世纪90年代开始该区域已成为无底栖生物区。因此对该区域进行重金属污染分析及其迁移规律的研究显得尤为重要。 本文通过对五里河河口区域水体及沉积物中四种重金属（Cu、Pb、Zn、Cd）的总量分析，采用单因子污染指数法和Hakanson生态风险指数法进行评价，发现该区域沉积物四种重金属中Cd污染最重，在河口区域尤其是河口口外海滨潮滩具有严重的潜在生态风险。对河口口外海滨潮滩沉积物中四种重金属的形态分布研究发现Cd的形态分布为离子可交换态＞碳酸盐结合态＞铁锰氧化物结合态＞有机质及硫化物结合态＞残渣态，基本处于活化状态。随后以河口口外海滨潮滩沉积物为基质进行了一系列的模拟实验，研究沉积物中Cd的迁移规律及机制。 以盐度和温度作为环境影响因子进行的静态模拟实验，结果显示：Cd污染沉积物在不同盐度与温度的水体中均能在24h左右达到最大释放量，使水体中Cd的浓度突然升高，造成水体Cd超标。室温条件下，不同盐度水体条件下沉积物中Cd的释放随盐度增加而增大，其中盐度为21.7‰和14.8‰的水体中出现两次释放高峰现象。恒温条件下在盐度相同的水体中，18℃和4℃的中、低温较29℃的高温更有利于沉积物Cd的释放，而温度相同的条件下则是21.7‰盐度的水体更利于沉积物Cd的释放，其次是海水盐度的水体。当外界条件相同时，温度恒定条件下Cd的释放大于室温条件。 从离子角度分析发现水体中的主要阴、阳离子可促进沉积物Cd的释放，其影响程度为Cl-＞Ca2+ > Mg2+> HCO3-＞SO42-≈Na+。 河口沉积物的环境质量、潜在生态风险及重金属的迁移特性分析均显示连山湾五里河河口区域具有很大的环境风险，应引起重视，并采取有效措施对其进行治理。 关键词 河口沉积物，重金属污染，Cd释放，环境风险

典型污染海湾河口沉积物中砷的形态分布研究

锦州湾是我国较大的浅水海湾之一，该湾西南五里河沿岸汇集数家工厂排污直接人海， 形成该湾口处的最大污染源。其西南部潮滩地段已成为我国海域污染最严重的区域之一。 本文对葫芦岛3条河流、五里河口和锦州湾水体、沉积物中As的含量、分布、形态及行为进行了系统研究。结果表明，葫芦岛的连山河和五里河未受到明显的As污染，沉积物总As含量一般低于10 mg•kg-1，河水As含量一般低于10 μg•L-1，茨山河下游紧邻葫芦岛锌厂，受到了严重的As污染，沉积物As含量达75.2 mg•kg-1。五里河口区As污染非常严重，锌厂在该区排污口处沉积物As含量高达3176.1 mg•kg-1，超过国家沉积物As标准158倍。受锌厂排污的影响锦州湾As污染也很严重，沉积物中As最高含量达到569.5 mg•kg-1。沉积物和孔隙水As含量在剖面上具有相似的变化趋势，表明孔隙水As含量主要受沉积物As含量影响。锦州湾沉积物中As主要以可交换态存在，残渣态次之，盐酸提取态最少，表明锦州湾沉积物中As的活性很强，有大量的可交换态As随时会进入上覆水体，是重要的As释放源，沉积物中大量As的释放可能会对葫芦岛和锦州湾地区生态系统造成严重的威胁。 以盐度和酸碱度作为环境影响因子进行的释放模拟实验，结果显示：不同盐度条件下，沉积物中的As均在48h左右达到最大释放量。此时，盐度为0‰的水体中As的释放量最大，而其他两种盐度的水体中As的释放量较小且二者相差不大。另外，在室温条件下，随着pH值的升高，沉积物中As的释放量逐渐增加。 通过对不同形态的单一含砷矿物的As提取效果研究得出：对于分级提取方法中吸附态砷的最佳提取条件应为1M KH2PO4 (pH5) 24h, 无定型铁氧化物上结合的砷0.2M oxalic/oxalate (pH3)避光 6h, 硫化亚铁上结合的砷，0.2M oxalic/oxalate (pH3)避光 1h。

A model study on carbon cycle and phytoplankton dynamical processes in the Bohai Sea

The carbon cycle of lower trophic level in the Bohai Sea is studied with a three-dimension-al biological and physical coupled model. The influences of the processes (including horizontal advection,river nutrient load, active transport etc. ) on the phytoplankton biomass and its evolution are estimated.The Bohai Sea is a weak sink of the CO2 in the atmosphere. During the cycle, 13.7% of the gross pro-duction of the phytoplankton enter the higher trophic level and 76.8 % of it are consumed by the respira-tion itself. The nutrient reproduction comes mainly from the internal biogeochemical loop and the rem-ineralization is an important mechanism of the nutrient transfer from organic form to inorganic. Horizon-tal advection decreases the total biomass and the eutrophication in some sea areas. Change in the nutrientload of a river can only adjust the local system near its estuary. Controlling the input of the nutrient,which limits the alga growth, can be very useful in lessening the phytoplankton biomass.

Iron and inorganic carbon in Liaodong Gulf sediments of Bohai Sea in China

Iron in seawater is an essential trace metal for phytoplankton that plays an important role in the marine carbon cycle. But most studies focused on oceanic iron fertilization in high nutrient low chlorophyll (HNLC) seawaters. A study of inorganic carbon (IC) forms and its influencing factors was presented in Liaodong Gulf sediments, and especially the influence of iron was discussed in detail. Inorganic carbon in Liaodong Gulf sediments was divided into five forms: NaCl, NH3·H2O, NaOH, NH2OH·HCl and HCl. The concentration of NaCl and NaOH forms were similar and they only occupied the minority of total inorganic carbon (TIC). However, NH3·H2O, NH2OH·HCl and HCl forms were the principal forms of TIC and accounted for more than 80% of TIC. Especially, the percentage of NH3·H2O form was much higher than that in the Changjiang River Estuary and Jiaozhou Bay sediments. All forms of inorganic carbon were influenced by organic carbon,pore water, iron, pH, redox potential(Eh) and sulfur potential(Es) in sediments, moreover, the influences had different characteristics for different IC forms. However, the redox reactions of iron affected mainly active IC forms. Iron had little effect on NH2OH·HCl and HCl forms of IC which were influenced mainly by pH. Iron had a stronger influence on NaCl, NaOH and NH3·H2O forms of IC; the influence of Fe2+ was higher than Fe3+ and its effect on NH3·H2O form was stronger than on NaCl and NaOH forms.

Concentrations of alkyphenol polyethoxylates entering UK estuaries

Concentrations of the weakly oestrogenic degradation products of alkylphenol polyethoxylate (APE) surfactants (nonylphenol, octylphenol, nonylphenol monoethoxylate and nonylphenol diethoxylate) were measured in water and sediments from British rivers and estuaries collected during 1994 and 1995. In addition, a series of samples of tissues of wild fish from the River Aire, and from a laboratory dosing experiment were analysed for alkylphenols, to assess the degree of bioaccumulation of these compounds. Measurable concentrations of APE residues were recorded in the River Aire (15–76 μg/l total extractable alkylphenols), the River Mersey (6–11 μg/l) and the Tees estuary (up to 76 μg/l). These levels exceed, or are close to, the no observed effect concentration for the induction of vitellogenesis in caged trout (5–20 μg/l total extractable alkylphenols), and may be sufficient to exert an oestrogenic effect on fish populations in these areas. A sediment sample from Bingley on the River Aire contained 15 μg/g (dry weight) nonylphenol, and concentrations in sediments from the Tees and Mersey estuaries exceeded 1 μg/g. These rivers receive a variety of trade waters via sewage treatment works (STW) effluents containing significant concentrations of APE. Elsewhere, concentrations in water and sediments were near or below limits of detection and biological effects are unlikely, suggesting that any oestrogenic effects observed in sewage outfalls and rivers not directly impacted by APE-containing trade-waters may be caused by other chemicals. Analysis of samples of trout muscle taken from a tank dosed at 65 μg/l nonylphenol indicated a bioaccumulation factor of between 90 and 125 after 3 weeks exposure. Samples of wild fish from the River Aire contained up to 0.8 μg/g nonylphenol in the muscle, a tissue bioaccumulation factor of approximately 50 relative to measured concentrations in water samples. A series of fish samples taken from offshore for food quality assurance purposes contained no detectable levels of APE residues (0.05–0.1 μg/g nonylphenol).

On-line voltammetric monitoring of dissolved Cu and Ni in the Gulf of Cadiz, south-west Spain

Geochemical processes in estuarine and coastal waters often occur on temporally and spatially small scales, resulting in variability of metal speciation and dissolved concentrations. Thus, surveys, which are aimed to improve our understanding of metal behaviour in such systems, benefit from high-resolution, interactive sampling campaigns. The present paper discusses a high-resolution approach to coastal monitoring, with the application of an automated voltammetric metal analyser for on-line measurements of dissolved trace metals in the Gulf of Cadiz, south-west Spain. This coastal sea receives metal-rich inputs from a metalliferous mining area, mainly via the Huelva estuary. On-line measurements of dissolved Cu, Zn, Ni and Co were carried out on-board ship during an eight-day sampling campaign in the study area in June 1997. A pumping system operated continuously underway and provided sampled water from a depth of ca. 4 m. Total dissolved metal concentrations measured on-line in the Gulf of Cadiz ranged between <5 nM Cu (<3 nM Ni) ca. 50 km off-shore and 60–90 nM Cu (5–13 nM Ni) in the vicinity of the Huelva estuary. The survey revealed steep gradients and strong tidal variability in the dissolved metal plume extending from the Huelva estuary into the Gulf of Cadiz. Further on-line measurements were carried out with the automatic metal monitor from the bank of the Odiel estuary over a full tidal cycle, at dissolved metal concentrations in the μM range. The application confirmed the suitability of the automated metal monitor for coastal sampling, and demonstrated its adaptability to a wide range of environmental conditions in the dynamic waters of estuaries and coastal seas. The near-real time acquisition of dissolved metal concentrations at high resolution enabled an interactive sampling campaign and therefore the close investigation of tidal variability in the development of the Huelva estuary metal plume.

Validation and in situ application of an automated dissolved nickel monitor for estuarine studies

The validation of a fully automated dissolved Ni monitor for in situ estuarine studies is presented, based on adsorptive cathodic stripping voltammetry (AdCSV). Dissolved Ni concentrations were determined following on-line filtration and UV digestion, and addition of an AdCSV ligand (dimethyl glyoxime) and pH buffer (N-2-hydroxyethylpiperazine-N′-2-ethanesulphonic acid). The technique is capable of up to six fully quantified Ni measurements per hour. The automated in situ methodology was applied successfully during two surveys on the Tamar estuary (south west Britain). The strongly varying sample matrix encountered in the estuarine system did not present analytical interferences, and each sample was quantified using internal standard additions. Up to 37 Ni measurements were performed during each survey, which involved 13 h of continuous sampling and analysis. The high resolution data from the winter and summer tidal cycle studies allowed a thorough interpretation of the biogeochemical processes in the studied estuarine system.

Interference effects in the extraction of trace metals from estuarine waters

We have found that a commonly used complexation and solvent extraction technique (using mixed dithiocarbamates/Freon/HNO3) does not always extract Cd, Co, Cu and Ni from estuarine samples with the same efficiency as from Milli-Q water. For samples collected from the Derwent Estuary (Australia), the reduced extraction efficiency only occurred for unfiltered samples, but low extraction efficiencies were also observed for a (filtered) riverine certified reference material (SLRS-3) suggesting that the effect may be widespread. We have not been able to identify the reason for the low extraction efficiency and, although it is strongly correlated with the presence of high concentrations of suspended solids, dissolved organic matter and particulate iron, we have no experimental evidence to directly link any of these parameters to the effect. It is possible that similar effects may occur in other techniques which rely on a preconcentration step prior to analysis and that some literature values of heavy metals in estuarine waters may be low. We propose a modification of the standard complexation/solvent extraction method which overcomes these difficulties without adding significantly to the time taken for analyses.