Characterization of cardiac-resident progenitor cells expressing high aldehyde dehydrogenase activity.

High aldehyde dehydrogenase (ALDH) activity has been associated with stem and progenitor cells in various tissues. Human cord blood and bone marrow ALDH-bright (ALDH(br)) cells have displayed angiogenic activity in preclinical studies and have been shown to be safe in clinical trials in patients with ischemic cardiovascular disease. The presence of ALDH(br) cells in the heart has not been evaluated so far. We have characterized ALDH(br) cells isolated from mouse hearts. One percent of nonmyocytic cells from neonatal and adult hearts were ALDH(br). ALDH(very-br) cells were more frequent in neonatal hearts than adult. ALDH(br) cells were more frequent in atria than ventricles. Expression of ALDH1A1 isozyme transcripts was highest in ALDH(very-br) cells, intermediate in ALDH(br) cells, and lowest in ALDH(dim) cells. ALDH1A2 expression was highest in ALDH(very-br) cells, intermediate in ALDH(dim) cells, and lowest in ALDH(br) cells. ALDH1A3 and ALDH2 expression was detectable in ALDH(very-br) and ALDH(br) cells, unlike ALDH(dim) cells, albeit at lower levels compared with ALDH1A1 and ALDH1A2. Freshly isolated ALDH(br) cells were enriched for cells expressing stem cell antigen-1, CD34, CD90, CD44, and CD106. ALDH(br) cells, unlike ALDH(dim) cells, could be grown in culture for more than 40 passages. They expressed sarcomeric α -actinin and could be differentiated along multiple mesenchymal lineages. However, the proportion of ALDH(br) cells declined with cell passage. In conclusion, the cardiac-derived ALDH(br) population is enriched for progenitor cells that exhibit mesenchymal progenitor-like characteristics and can be expanded in culture. The regenerative potential of cardiac-derived ALDH(br) cells remains to be evaluated.

Iowa Community Empowerment Newsletter, August 2004, Vol. 6, no. 4

Monthly newsletter for the Iowa Department of Public Health

Opinion de C. Marbot, sur la résolution relative aux pensions des veuves et des enfans des défenseurs de la patrie : séance du 14 fructidor an 6 ([Reprod.]) / [publ. par le] Conseil des Anciens

Collection : Les archives de la Révolution française ; 11.1a.446

ABD e-News August 6, 2004

Weekly newsletter for the State of Iowa Alcoholic Beverages Division

Iowa Technology, July, 2002, Issue 6

Newsletter produced by the Iowa Information Technology Department

Credit Union Division Activities, April 2003, Issue 6

Newsletter for Iowa Credit Union Division

Estudi dels fenòmens de contacte de llengües en el lèxic d'escolars de primera llengua castellana i la seva l'evolució lingüística (de 6è de primària a 1r d'ESO)

Aquest treball estudia els fenòmens de contacte de llengües que apareixen a la llengua oral catalana d'una mostra d'adolescents de primera llengua castellana de Manlleu en dos punts temporals (6è de primària i 1r d'ESO) i compara els resultats obtinguts amb dos estudis precedents (Vila, 1996; Galindo, 2006)

Histoire du dix-neuvième siècle depuis les traités de Vienne. Tome 6 / G. G. Gervinus,... ; traduit de l'allemand par J.-F. Minssen,...

Collection : Collection d'historiens contemporains

Iowa Community Empowerment Newsletter, October 2003, Vol. 4, No. 6

Monthly newsletter for the Iowa Department of Public Health

Iowa Community Empowerment Newsletter, December 2002, Vol. 3, no. 6

Monthly newsletter for the Iowa Department of Public Health

Iowa Community Empowerment Newsletter, December 2001, Vol. 2, no. 6

Monthly newsletter for the Iowa Department of Public Health

Species-Specific Recognition of Aspergillus fumigatus by Toll-like Receptor 1 and Toll-like Receptor 6.

Background. Aspergillus fumigatus causes invasive aspergillosis, a potentially fatal infection in oncohematological patients. Innate immune detection of A. fumigatus involves Toll-like receptor (TLR) 4 and TLR2, which forms a heterodimer with either TLR1 or TLR6. The role of those coreceptors in Aspergillus sensing is unknown. Methods. Cytokine production was measured in bone marrow-derived macrophages (BMDMs) from wild-type (WT) and TLR-deficient mice after incubation with a WT and an immunogenic RodA-deficient (ΔrodA-47) strain of A. fumigatus and in lungs from these mice after intranasal mold inoculation. Aspergillus fumigatus-mediated NF-κB activation was measured in HEK293T cells transfected with plasmids expressing mouse or human TLRs. Results. Bone marrow-derived macrophages from TLR1- and TLR6-deficient mice produced lower amounts of interleukin 12p40, CXCL2, interleukin 6, and tumor necrosis factor α than BMDMs from WT mice after stimulation with A. fumigatus. Lungs from TLR1- and TLR6-deficient mice had diminished CXCL1 and CXCL2 production and increased fungal burden after intranasal inoculation of ΔrodA A. fumigatus compared with lungs from WT mice. ΔrodA strain-mediated NF-κB activation was observed in HEK293T cells expressing mouse TLR2/1, mouse TLR2/6, and human TLR2/1 but not human TLR2/6. Conclusions. Innate immune detection of A. fumigatus is mediated by TLR4 and TLR2 together with TLR1 or TLR6 in mice and TLR1 but not TLR6 in humans.