Friday Facts, September 17, 2010, Vol. 71

Bureau of Nutrition and Health Promotion part of the Iowa Department of Public Health produces of weekly newsletter about the Iowa WIC Program for the State of Iowa citizen.

Friday Facts, December 17, 2010, Vol. 83

Bureau of Nutrition and Health Promotion part of the Iowa Department of Public Health produces of weekly newsletter about the Iowa WIC Program for the State of Iowa citizen.

Agri-News, August 17, 2006, Vol. 6, no. 16

Crop and livestock summaries for the state of Iowa, produced by the Iowa Department of Agriculture.

Agri-News, August 22, 2006, Vol. 6, no. 17

Crop and livestock summaries for the state of Iowa, produced by the Iowa Department of Agriculture.

EPI Update, June 17, 2011

Weekly newsletter for Center For Acute Disease Epidemiology of Iowa Department of Public Health.

Agri-News, September 17, 2010, Vol. 10, no. 16

Crop and livestock summaries for the state of Iowa, produced by the Iowa Department of Agriculture.

Agri-News, October 8, 2010, Vol. 10, no. 17

Crop and livestock summaries for the state of Iowa, produced by the Iowa Department of Agriculture.

Agri-News, October 16, 2009, Vol. 9, no. 17

Crop and livestock summaries for the state of Iowa, produced by the Iowa Department of Agriculture.

Agri-News, October 17, 2008, Vol. 8, no. 16

Crop and livestock summaries for the state of Iowa, produced by the Iowa Department of Agriculture.

Agri-News, November 7, 2008, Vol. 8, no. 17

Crop and livestock summaries for the state of Iowa, produced by the Iowa Department of Agriculture.

Agri-News, November 6, 2007, Vol. 7, no. 17

Crop and livestock summaries for the state of Iowa, produced by the Iowa Department of Agriculture.

Sequence analysis and expression of the attachment and fusion proteins of canine distemper virus wild-type strain A75/17.

The biological properties of wild-type A75/17 and cell culture-adapted Onderstepoort canine distemper virus differ markedly. To learn more about the molecular basis for these differences, we have isolated and sequenced the protein-coding regions of the attachment and fusion proteins of wild-type canine distemper virus strain A75/17. In the attachment protein, a total of 57 amino acid differences were observed between the Onderstepoort strain and strain A75/17, and these were distributed evenly over the entire protein. Interestingly, the attachment protein of strain A75/17 contained an extension of three amino acids at the C terminus. Expression studies showed that the attachment protein of strain A75/17 had a higher apparent molecular mass than the attachment protein of the Onderstepoort strain, in both the presence and absence of tunicamycin. In the fusion protein, 60 amino acid differences were observed between the two strains, of which 44 were clustered in the much smaller F2 portion of the molecule. Significantly, the AUG that has been proposed as a translation initiation codon in the Onderstepoort strain is an AUA codon in strain A75/17. Detailed mutation analyses showed that both the first and second AUGs of strain A75/17 are the major translation initiation sites of the fusion protein. Similar analyses demonstrated that, also in the Onderstepoort strain, the first two AUGs are the translation initiation codons which contribute most to the generation of precursor molecules yielding the mature form of the fusion protein.