Imunidade celular em caninos neonatos - do nascimento ao 45° dia de idade

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo da viabilidade econômica da coleta seletiva e da compostagem dos resíduos sólidos do município de Guaratinguetá (SP)

Pós-graduação em Engenharia Civil e Ambiental - FEB

Viabilidade do parasitismo por Haemonchus contortus em bovinos experimentalmente infectados

Pós-graduação em Zootecnia - FCAV

Características da base de conhecimentos de professores no ensino de biologia celular a partir de um curso de formação continuada

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo da viabilidade técnica, econômica e ambiental da substituição de um banco de resistências elétricas de aquecedores por uma caldeira alimentada por biomassa de paletes residuais na indústria de laminação

Given the increasing demand for fossil fuels to power the engines of the global economy, and also the slow process of synthesis of oil, it becomes necessary to develop new sources of renewable energy in addition to improve promising sources that already exist, turning them competitive. Based on this, biomass is shown very promising between the most popular energy sources due to its availability, applicability, and above all, the fact of not emitting green house gases when analyzing the whole carbon cycle. In this sense, this work presents, through an environmental, technical and economic analysis, the impact in an aluminum mill of replacing the use of electricity, in one process, by a boiler fueled with biomass from waste process of packaging the finished product with pallets. Thus, besides the reduction of CO2 emissions, financial gains are targeted, since the main goal of the corporations that require such power is to maximize its profits

Avaliação dos efeitos dos inseticidas malation e fipronil sobre a viabilidade e regeneração de girardia tigrina (Platyhelminthe, tricladida)

In this study were carried out toxicity tests and the evaluation of sub lethal effects of the insecticides malathion and fipronil, over the test system of Girardia tigrina, using for these analyses a great variety of methods. For evaluation of the lethal concentrations of the products, adult planarians were exposed to several different concentrations. All the tests were carried out in duplicate, maintained on the shelter of the light, on a temperature 24-26ºC and they had the duration of 96h, with observations at each 24h. The results were statically valued by the tests U of Mann- Whitney, Spearman-Karber, ANOVA and Pearson correlation. The effects over the system-test, in the different tested concentrations, varied in accordance with the time of exposition. The EC50 given by the test of Spearman-Karber was of 12,5mg/L for malathion and 13,4mg/L for fipronil. The evaluation of sub lethal effects was composed by analyses of the potential of regeneration; histological, for tissue damage evaluation; d; and the “comet assay”, for DNA damage detection. The specimens split up and exposed to the insecticides demonstrated, between other effects, retardation in development of the regenerative blastema. The histological evaluation has shown that exposed planarians to lower concentrations of the insecticides cause more significant damage against the tissues and some specific cell types. Through the comet assay, significant damages were noticed in the DNA in all the treatments, when compared with the control group. Such damages may result in reproduction and immunity alterations, which reduce the competitive efficiency and, consequently, the survival chances of these organisms.

Análise da viabilidade de simulações com feixes de prótons de 150MeV em alvos heterogêneos

A protonterapia é um tipo de tratamento promissor quanto a diminuição de dose nos tecidos adjacentes ao tumor, devido às suas característica de deposição de energia ao interagir com a matéria. Para aumentar a acurácia do tratamento é necessário que as simulações sejam feitas a partir de um sistema de formação de imagem que utilize também um feixe de prótons, como a tomografia por feixe de prótons (pCT). Essa área atualmente está em desenvolvimento e se defronta com inúmeros problemas na formação de uma imagem precisa. Este trabalho tem o intuito de analisar a viabilidade de simulações computacionais para este tipo de feixe. O código computacional SRIM é um conjunto de programas que calcula o freamento e o alcance de íons ao interagir com a matéria. Esse cálculo baseia-se em ferramentas oriundas da mecânica quântica e também no método de Monte Carlo, para determinar, de modo probabilístico, trajetórias, colisões e ângulos. Foram realizadas simulações no programa SRIM para um alvo heterogêneo envolvendo água e osso cortical. Para analisar o comportamento do feixe transmitido, foram feitas alterações na posição e no tamanho da heterogeneidade. A partir dos dados obtidos pode-se obter valores médios, histogramas e gaussianas dos mesmos para cada simulação e tirar conclusões sobre a viabilidade e as dependências desse tipo de simulação

Indução de morte celular por apoptose decorrente da irradiação com Raios-X: um ensaio com células mamárias humanas tumorais

Apoptosis is a form of programmed cell death selectively removes abnormal cells, and thus contributes to maintaining the balance of the dynamics of cell reproduction. Therefore the verification of the occurrence of apoptotic cell death after a pathological stimulus is crucial for the analysis of the maintenance of normal cell cycle of a given tissue or organ. In this experiment were used cells lines human mammary tumor MDAMB231, T47, MCF7, which were irradiated with X-rays at a dose of 5 Gy in a time interval of 15 seconds, and filtration of 1mm aluminum. Samples containing the cells were grown in a specific culture medium, containing fetal bovine serum and growth factor, and two samples were prepared with each of the cell lines, one to be irradiated, and another that has not been irradiated, which denoted by negative control of the irradiation. The primary goal of the experiment was to verify and compare the rates of apoptosis in each cell lines, in which were irradiated and that were not irradiated, using flow cytometry as a method for detecting apoptotic cell death in together with specific markers annexin V and propidium iodide. Data from the readings made by flow cytometry were analyzed and interpreted using the software WinMDI statistical graph. By comparing the indices relating to the readings of positive and negative for specific markers of apoptosis, based on differences in the statistical data presented lectures regarding the cellular irradiated and not irradiated, collude cells in question once... (Complete abstract click electronic access below)

Estudo da viabilidade de uma gestão ambiental de pilhas no campus da Unesp de Rio Claro

When exhausted, the batteries are classified as hazardous waste because it has potentially toxic heavy metals and bio-accumulators, such as mercury, cadmium and lead in their internal composition functional. Due to the high level of danger, such waste should be properly designed to not allow theses chemical components to cause significant impacts on the environment. Thus, the recycling of waste in question is the best destination. In this context, was wanted to know, through a questionnaire in interview format, how the graduates of the Universidade Estadual Paulista Julio de Mesquita Filho of Rio Claro / SP are involved with this environmental issue. Since then, has been developed and implemented an environmental management for internal batteries

Análise da expressão protéica de moléculas de adesão celular em melanomas cutâneos

Cutaneous melanoma is a malignancy caused by the uncontrolled proliferation of melanocytes. Has been increasing in incidence and mortality over the years. This type of tumor may arise in the skin or any other place where there was migration of neural crest cells. Melanocytes are located in the basal layer of the epidermis forming the epidermal melanin unit, involving a melanocyte to 36 keratinocytes. The proliferation of melanocytes depends on this relationship with the keratinocytes, gap junctions, E-cadherin and desmoglein. The basal membrane is responsible for promoting the integrity of the skin, their adhesion structures shown to be altered in several skin diseases. Some molecules that play the role of adherence in this membrane are the laminins, type IV collagen and type XVII and perlecan. Analysis of protein expression of cell adhesion molecules in melanoma by immunohistochemistry on tissue microarray (TMA). We selected paraffin blocks of patients who were diagnosed with cutaneous melanoma in the period 1995 to 2010, reaching a sample of 124 cases. All these have gone through review of preparation for subsequent histological analysis of tissue microarray (TMA). The protein expression will be analyzed by immunohistochemistry, using markers anti-laminin, anti-collagen type IV and type XVII and anti-perlecan. There was a predominance of female patients, Caucasians and southeastern Brazil. There were frequent in type of extensive superficial ulcers with Breslow depth greater than 4 mm, Clark level IV and mitotic index greater than 6 mitoses per cga. The patients, most of them were female with mean age of 57.13 years and Caucasian. The most frequent histological type was extensive superficial followed by acral lentiginous. Predominated cases very deep, with Breslow thickness greater than 4 mm, Clark IV, ulcerated and on average by 9 cga mitosis, leading to worse prognosis in this sample. It...(Complete abstract click electronic access below)

Análise da viabilidade de motorização elétrica do soprador no ciclo regenerativo da unidade de craqueamento catalítico fluidizado em uma refinaria

One of the biggest environmental problems of today is the climate change. Experts affirm that this global warming is related to the greenhouse effect. Its causes are directly related to human activity, especially the use of fossil fuels. In this context, companies around the world are challenged to improve energy efficiency in order to reduce the environmental impact and work toward the so-called tripod of sustainable development that focuses on the social, economic and environmental aspects of a business strategy. The first step a company can make in this regard is to conduct an inventory of emissions of greenhouse gases (GHGs). The reduction of GHG emissions in a refinery can be achieved by replacing steam turbines with electric motors to drive big machines, this reduction is achieved by relieving the steam consumption for electric power available or purchased. An important aspect associated with the reduction of GHG emissions is the best performance of the Energy Intensity Index (ERI). The objective of this study was to analyze the feasibility of the blower motorization in the regenerative cycle of a fluidized catalytic cracking unit at a specific refinery. For development work, two methods were used, the initial screening and optimization scenarios with the help of software Butyl. The results indicate that after a certain cost of natural gas this substitution becomes favorable. In addition, there is a large reduction of CO2 emissions avoided by burning fuel

Desenvolvimento de equipamento LED para aplicação em cultura celular

O estágio realizado em parceria entre o Departamento de Física e Biofísica do IBB-UNESP e o Hemocentro de Botucatu da FMB-UNESP teve como objetivo desenvolver um novo equipamento de luz LED capaz de interagir com culturas celulares. Desta forma foi desenvolvido um equipamento com luz azul e luz vermelha. A luz azul é responsável por inibir o crescimento celular, a luz vermelha é responsável por aumentar o crescimento celular. O equipamento consiste de uma fonte, e dois conjuntos com três LEDs cada. A cultura celular escolhida foi a de Miemola Murino (NS1), uma linhagem que possui alta taxa de crescimento e que foi mantida em estufa e meio de cultura, (simulando condições normais de sobrevivência), sendo irradiada três vezes ao dia. As amostras foram coletadas e contadas em câmara de Neubauer. Os resultados obtidos, quando comparados com o controle, não apresentaram significativa diferença de crescimento quando irradiados com luz LED vermelho. Porém quando comparados o grupo controle com o grupo irradiado com LED azul, notou-se uma significativa redução da quantidade de células a partir do sétimo dia de irradiação. Acredita-se que a ineficácia dos LEDs vermelhos ocorreram pois utilizamos células com metabolismo bastante elevado, dificultando um aumento ainda maior da taxa de crescimento. A redução da população de células azuis ocorre pois a radiação interage com a membrana celular, causando a quebra dessa membrana e conseqüente morte da célula. Outra via para a morte é a lesão causada no DNA das células, que ocorre quando a luz azul é absorvida pela molécula

Viabilidade de turbinas eólicas de pequeno porte no Brasil

Facing a global scenario where oil is scarce and the emission of CO2 in the atmosphere is a world concern, the search for new methods of energy production becomes the focus of all countries. Based on these assumptions, the wind power gains highlight and happen to be the most exploited around the world, since this does not produce toxic waste, since it uses the wind, no raw material shortage and its cost and efficiency are increasingly attractive. Taking advantage of the geographical features of Brazil, coastal and territorial extension, this study aims to analyze the feasibility of using wind turbines widely used in Germany, one of the countries with the highest production of energy through wind, in Brazil. In order to increase the energy production without causing more environmental impacts, geographical analyses will be made, and to determine the features and costs of production of this type of energy generation, and at the end of this dissertation, will finish for one of the turbines, Montana, is it feasible to install, however due to the Government's little incentive return is not very expressive. With respect to the turbine Passaat, this is infeasible to install under current conditions

Avaliação da viabilidade econômica do uso de um aquecedor solar de água de garrafas pet para aquecer o ar de uma cabine de pintura e uma estufa de secagem

Nowadays, the biggest part of the world's energy supply comes from fossil fuels and nuclear. However, the current need of the society for the preservation of the environment and wise use of natural resources, has favored the search for alternative energy sources and improvement of energy efficiency. In this new scenario, companies are beginning to mobilize in order to adapt its facilities to renewable energy. Solar, with its immense potential, not really exploited yet, can be very useful for companies that want to beat their sustainability goals. Given these facts, the aim is to evaluate the economic viability of introducing a solar water heater which uses a colorless PET bottle as one of its components in a plant. The hot water generated will heat the air of a paint booth and a warm house, reducing energy consumption, since they are heated by electric heaters

A simulação de Monte Carlo como instrumento fundamental para a análise de viabilidade econômica: a implementação de uma fundição de ferro em uma empresa metalúrgica

In uncertainly economic scenarios, an economic feasibility analysis must be done to accept a project based on investment criteria, such as NPV and IRR, mainly because the shareholders tend to invest their budget in a project if it has a great chance to return their investments. The studied company outsources all of its foundry services, what makes it very dependent of its suppliers, because the products have a lower quality level, long delivery periods and high prices. Therefore, this work will analyze the project of building an iron-casting foundry to decrease the dependence of its suppliers. In order to develop this research, all needed data related to the construction of the foundry and sales were collected to create deterministic and probabilistic (Monte Carlo Simulation) cash flows using MsExcel® and Oracle's Crystal Ball® software. As a result, the project was found to be risky by the NPV and IRR in the case of this new production line supplying only the internal needs. However, when the company offers its services to the foundry market, the project turns to be feasible