Routine histological technique applied to a biological model for environmental studies

Introduction and aim Earthworms are an important test organisms used in several standard ecotoxicological tests (e.g. ISO, 2008, 2012; OECD, 2004, 1094) and they are also model organisms to test soil structure and permeability, as well as for important soil services as the degradation organic matter. Although histopathological changes have been demonstrated to be good biomarkers to assess the exposure of these animals to different physical and chemical stress agents, studies with clear and high quality images describing normal tissue conditions are scarce in the literature, so the aim of this work was to better characterize this biological model. Material and Methods Eight adult earthworms exposed to an artificial standard soil (OECD, 2004) for 28 days, were extracted and placed in a plastic box to depurate their gut content, fixed in 10% neutral-buffered formalin and processed for routine histopathological diagnosis. Results and discussion Satisfactory histological sections were obtained. Some difficulties were faced related with microtome sectioning, resulting in artefacts, namely lines across sections produced by a nick in the cutting edge of the microtome knife cutting tear, motivated by the presence of sand and other solid particles that persisted in the gut of earthworms. Nevertheless, it was possible to obtain representative figures from different earthworm sections. Conclusion Routine histological technique was effective for obtaining satisfactory histological sections and the knowledge of the histology of earthworms could be very useful for future application in environmental studies, using this biological model.

Occurrence of planktic foraminifera in Pliocene to Holocene sediments of DSDP Hole 81-552A in the North Atlantic (Appendix 2)

A new planktic foraminifer transfer function (GSF18) related 5 North Atlantic assemblages to winter and summer sea surface temperature. GSF18, based on recombined and simplified core top census data, preserves most environmental information and reproduces modern North Atlantic conditions with approximately the same accuracy as previous transfer functions, but can be more readily applied to faunal samples ranging in age from Pliocene to Holocene. Transfer function GSF18 has been applied to faunal data from Deep Sea Drilling Project Hole 552A to produce a 2.5 m.y. sea-surface temperature (SST) time series. Estimates show several periods between 2.3 and 4.6 Ma during which mean SST's were both several degrees warmer and several degrees cooler than modern conditions. Between 2.9 and 4.0 Ma SST was generally warmer than modern except for a 250 k.y. interval centered at 3.3 Ma. Maximum SST, with respect to modern conditions, occurred after the cool interval near 3.1 Ma when SST was approximately 3.6° C warmer than present conditions. Comparison of SST estimates with stable isotope data suggest that after peak warming at 3.1 Ma, there was an overall surface water cooling with concomitant build up of global ice volume, culminating in Northern Hemisphere glaciation. This event is also indicated by the presence of ice rafted detritus in 552A sediments at about 2.45 Ma.

Preliminary risk assessment for parasites in municipal sewage sludge applied to land.

Mode of access: Internet.

Catalytic conversion of solvent refined coal to liquid products /

"LBL-6807."

Landscape gardening as applied to school grounds.

"Prepared by Prof. Tell W. Nicolet."--p. [3].

Horse-breeding, being the general principles of heredity applied to the business of breeding horses, with instructions for the management of stallions, brood mares and young foals, and the selection of breeding stock,

Mode of access: Internet.

Blood group antigens & antibodies as applied to blood transfusion.

Includes bibliographical references.

Lessons learned from public health campaigns and applied to anti-DWI norms development. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Sketches, graphic and descriptive, &c. for a history of the decorative painting applied to English architecture during the middle ages.

Binder's title: History of decorative painting.

Plane waves and spherical means applied to partial differential equations.

Bibliography: p. 165-170.

Hazardous wastes : a risk-benefit framework applied to cadmium and asbestos /

SRI project no. EGU-3561 ; EPA contract no. 68-01-2915

Experimental and formal language design applied to control constructs for interactive computing /

Vita.

Coding and flow charting as applied to UICSM programed texts (1962-1963 versions) /

Project No. 711151.01.

Principles and problems of pattern making as applied to women's apparel,

Mode of access: Internet.