FIWARE Lab: managing resources and services in a cloud federation supporting future internet applications

Real-world experimentation facilities accelerate the development of Future Internet technologies and services, advance the market for smart infrastructures, and increase the effectiveness of business processes through the Internet. The federation of facilities fosters the experimentation and innovation with larger and more powerful environment, increases the number and variety of the offered services and brings forth possibilities for new experimentation scenarios. This paper introduces a management solution for cloud federation that automates service provisioning to the largest possible extent, relieves the developers from time-consuming configuration settings, and caters for real-time information of all information related to the whole lifecycle of the provisioned services. This is achieved by proposing solutions to achieve the seamless deployment of services across the federation and ability of services to span across different infrastructures of the federation, as well as monitoring of the resources and data which can be aggregated with a common structure, offered as an open ecosystem for innovation at the developers' disposal. This solution consists of several federation management tools and components that are part of the work on Cloud Federation conducted within XIFI project to build the federation of cloud infrastructures for the Future Internet Lab (FIWARE Lab). We present the design and implementation of the solution-concerned FIWARE Lab management tools and components that are deployed within a federation of 17 cloud infrastructures distributed across Europe.

Automated parallel DNA sequencing on multiple channel microchips

We report automated DNA sequencing in 16-channel microchips. A microchip prefilled with sieving matrix is aligned on a heating plate affixed to a movable platform. Samples are loaded into sample reservoirs by using an eight-tip pipetting device, and the chip is docked with an array of electrodes in the focal plane of a four-color scanning detection system. Under computer control, high voltage is applied to the appropriate reservoirs in a programmed sequence that injects and separates the DNA samples. An integrated four-color confocal fluorescent detector automatically scans all 16 channels. The system routinely yields more than 450 bases in 15 min in all 16 channels. In the best case using an automated base-calling program, 543 bases have been called at an accuracy of >99%. Separations, including automated chip loading and sample injection, normally are completed in less than 18 min. The advantages of DNA sequencing on capillary electrophoresis chips include uniform signal intensity and tolerance of high DNA template concentration. To understand the fundamentals of these unique features we developed a theoretical treatment of cross-channel chip injection that we call the differential concentration effect. We present experimental evidence consistent with the predictions of the theory.

Effects of receptor dimerization on the interaction between the class I major histocompatibility complex-related Fc receptor and IgG.

The neonatal Fc receptor (FcRn) transports maternal IgG from ingested milk in the gut to the bloodstream of newborn mammals. An FcRn dimer was observed in crystals of the receptor alone and of an FcRn-Fc complex, but its biological relevance was unknown. Here we use surface plasmon resonance-based biosensor assays to assess the role of FcRn dimerization in IgG binding. We find high-affinity IgG binding when FcRn is immobilized on a biosensor chip in an orientation facilitating dimerization but not when its orientation disrupts dimerization. This result supports a model in which IgG-induced dimerization of FcRn is relevant for signaling the cell to initiate endocytosis of the IgG-FcRn complex.

Real-time detection of DNA hybridization and melting on oligonucleotide arrays by using optical wave guides.

The challenge of the Human Genome Project is to increase the rate of DNA sequence acquisition by two orders of magnitude to complete sequencing of the human genome by the year 2000. The present work describes a rapid detection method using a two-dimensional optical wave guide that allows measurement of real-time binding or melting of a light-scattering label on a DNA array. A particulate label on the target DNA acts as a light-scattering source when illuminated by the evanescent wave of the wave guide and only the label bound to the surface generates a signal. Imaging/visual examination of the scattered light permits interrogation of the entire array simultaneously. Hybridization specificity is equivalent to that obtained with a conventional system using autoradiography. Wave guide melting curves are consistent with those obtained in the liquid phase and single-base discrimination is facile. Dilution experiments showed an apparent lower limit of detection at 0.4 nM oligonucleotide. This performance is comparable to the best currently known fluorescence-based systems. In addition, wave guide detection allows manipulation of hybridization stringency during detection and thereby reduces DNA chip complexity. It is anticipated that this methodology will provide a powerful tool for diagnostic applications that require rapid cost-effective detection of variations from known sequences.

Modelagem e simulação de redes em chip sem fio.

O paradigma das redes em chip (NoCs) surgiu a fim de permitir alto grau de integração entre vários núcleos de sistemas em chip (SoCs), cuja comunicação é tradicionalmente baseada em barramentos. As NoCs são definidas como uma estrutura de switches e canais ponto a ponto que interconectam núcleos de propriedades intelectuais (IPs) de um SoC, provendo uma plataforma de comunicação entre os mesmos. As redes em chip sem fio (WiNoCs) são uma abordagem evolucionária do conceito de rede em chip (NoC), a qual possibilita a adoção dos mecanismos de roteamento das NoCs com o uso de tecnologias sem fio, propondo a otimização dos fluxos de tráfego, a redução de conectores e a atuação em conjunto com as NoCs tradicionais, reduzindo a carga nos barramentos. O uso do roteamento dinâmico dentro das redes em chip sem fio permite o desligamento seletivo de partes do hardware, o que reduz a energia consumida. Contudo, a escolha de onde empregar um link sem fio em uma NoC é uma tarefa complexa, dado que os nós são pontes de tráfego os quais não podem ser desligados sem potencialmente quebrar uma rota preestabelecida. Além de fornecer uma visão sobre as arquiteturas de NoCs e do estado da arte do paradigma emergente de WiNoC, este trabalho também propõe um método de avaliação baseado no já consolidado simulador ns-2, cujo objetivo é testar cenários híbridos de NoC e WiNoC. A partir desta abordagem é possível avaliar diferentes parâmetros das WiNoCs associados a aspectos de roteamento, aplicação e número de nós envolvidos em redes hierárquicas. Por meio da análise de tais simulações também é possível investigar qual estratégia de roteamento é mais recomendada para um determinado cenário de utilização, o que é relevante ao se escolher a disposição espacial dos nós em uma NoC. Os experimentos realizados são o estudo da dinâmica de funcionamento dos protocolos ad hoc de roteamento sem fio em uma topologia hierárquica de WiNoC, seguido da análise de tamanho da rede e dos padrões de tráfego na WiNoC.

The Impact of Prefrontal Cortex "Warm Up" on Immediate Cognitive Reappraisal Ability in Older Adolescents with Elevated Symptoms of Depression

Cognitive Reappraisal (CR) is a central component of Cognitive Behavioral Therapy for adolescent depression. Yet, previous research indicates that a brain region highly associated with successful CR in adults, the Prefrontal Cortex (PFC), is not fully developed until early adulthood. Thus, there is growing concern that CBT interventions directed at building CR abilities in depressed teens might be constrained by PFC immaturity. However, CR is an effective strategy for regulating affect. The current study evaluated an intervention aimed at enhancing CR performance through PFC “warm up” with a working memory task. Additionally, the study examined moderators of intervention response, as well as cognitive correlates of self-reported CR use. Participants included 48 older adolescents (mean age=19.1, 89% female) with elevated symptoms of depression who were randomly assigned to a lab-based WM or control activity followed by a CR task. Overall, results failed to support the effectiveness of “warm up” to augment CR performance. However, current level of depression predicted negative bias and sadness ratings after CR instructions, and this effect was qualified by an interaction with condition. The moderator analysis showed that depressive symptoms interacted with condition such that in the control condition, participants with higher depressive symptoms had significantly lower negative bias scores than individuals with lower depressive symptoms, but this pattern was not found in the experimental condition. Contrary to hypotheses, history of depression did not moderate treatment response. Additional analyses explored alternative explanations for the lack of intervention effects. There was some evidence to suggest that the WM task was frustrating and cognitively taxing. However, irritation scores and overall WM task accuracy did not predict subsequent CR performance. Lastly, multiple cognitive variables emerged as correlates of self-reported CR use, with cognitive flexibility contributing unique variance to self-reported CR use. Results pointed to new directions for improving CR performance among youth with elevated symptoms of depression.

A Survey on FPGA-Based Sensor Systems: Towards Intelligent and Reconfigurable Low-Power Sensors for Computer Vision, Control and Signal Processing

The current trend in the evolution of sensor systems seeks ways to provide more accuracy and resolution, while at the same time decreasing the size and power consumption. The use of Field Programmable Gate Arrays (FPGAs) provides specific reprogrammable hardware technology that can be properly exploited to obtain a reconfigurable sensor system. This adaptation capability enables the implementation of complex applications using the partial reconfigurability at a very low-power consumption. For highly demanding tasks FPGAs have been favored due to the high efficiency provided by their architectural flexibility (parallelism, on-chip memory, etc.), reconfigurability and superb performance in the development of algorithms. FPGAs have improved the performance of sensor systems and have triggered a clear increase in their use in new fields of application. A new generation of smarter, reconfigurable and lower power consumption sensors is being developed in Spain based on FPGAs. In this paper, a review of these developments is presented, describing as well the FPGA technologies employed by the different research groups and providing an overview of future research within this field.

Assessment of CO2 adsorption capacity on activated carbons by a combination of batch and dynamic tests

In this work, batch and dynamic adsorption tests are coupled for an accurate evaluation of CO2 adsorption performance for three different activated carbons obtained from olives stones by chemical activation followed by physical activation with CO2 at varying times, i.e. 20, 40 and 60 h. Kinetic and thermodynamic CO2 adsorption tests from simulated flue-gas at different temperature and CO2 pressure are carried out both in batch (a manometric equipment operating with pure CO2) and dynamic (a lab-scale fixed-bed column operating with CO2/N2 mixture) conditions. The textural characterization of the activated carbon samples shows a direct dependence of both micropore and ultramicropore volume on the activation time, hence AC60 has the higher contribution. The adsorption tests conducted at 273 and 293 K showed that, when CO2 pressure is lower than 0.3 bar, the lower the activation time the higher CO2 adsorption capacity and a ranking ωeq(AC20)>ωeq(AC40)>ωeq(AC60) can be exactly defined when T= 293 K. This result can be likely ascribed to a narrower pore size distribution of the AC20 sample, whose smaller pores are more effective for CO2 capture at higher temperature and lower CO2 pressure, the latter representing operating conditions of major interest for decarbonation of a flue-gas effluent. Moreover, the experimental results obtained from dynamic tests confirm the results derived from the batch tests in terms of CO2 adsorption capacity. It is important to highlight that the adsorption of N2 on the synthesized AC samples can be considered negligible. Finally, the importance of a proper analysis of characterization data and adsorption experimental results is highlighted for a correct assessment of CO2 removal performances of activated carbons at different CO2 pressure and operating temperature.

Sedimentological, mineralogical, biogeochemical and micropaleontological parameters on sediment core PS69/849-2, Burton Basin, MacRobertson Shelf, East Antarctica

A multi-proxy study including sedimentological, mineralogical, biogeochemical and micropaleontological methods was conducted on sediment core PS69/849-2 retrieved from Burton Basin, MacRobertson Shelf, East Antarctica. The goal of this study was to depict the deglacial and Holocene environmental history of the MacRobertson Land-Prydz Bay region. A special focus was put on the timing of ice-sheet retreat and the variability of bottom-water formation due to sea ice formation through the Holocene. Results from site PS69/849-2 provide the first paleo-environmental record of Holocene variations in bottom-water production probably associated to the Cape Darnley polynya, which is the second largest polynya in the Antarctic. Methods included end-member modeling of laser-derived high-resolution grain size data to reconstruct the depositional regimes and bottom-water activity. The provenance of current-derived and ice-transported material was reconstructed using clay-mineral and heavy-mineral analysis. Conclusions on biogenic production were drawn by determination of biogenic opal and total organic carbon. It was found that the ice shelf front started to retreat from the site around 12.8 ka BP. This coincides with results from other records in Prydz Bay and suggests warming during the early Holocene optimum next to global sea level rise as the main trigger. Ice-rafted debris was then supplied to the site until 5.5 cal. ka BP, when Holocene global sea level rise stabilized and glacial isostatic rebound on MacRobertson Land commenced. Throughout the Holocene, three episodes of enhanced bottom-water activity probably due to elevated brine rejection in Cape Darnley polynya occured between 11.5 and 9 cal. ka BP, 5.6 and 4.5 cal. ka BP and since 1.5 cal. ka BP. These periods are related to shifts from warmer to cooler conditions at the end of Holocene warm periods, in particular the early Holocene optimum, the mid-Holocene warm period and at the beginning of the neoglacial. In contrast, between 7.7 and 6.7 cal. ka BP, brine rejection shut down, maybe owed to warm conditions and pronounced open-water intervals.

Sedimentology and paleontology on core Lz1104 from Lake Hjort, Greenland

Two sediment cores of 70 and 252 cm length were recovered from Hjort Sø, a small lake on Store Koldewey, Northeast Greenland, and studied with a multidisciplinary approach in order to reconstruct the local environmental history and to test the relevance of proxies for paleoenvironmental information. The basal sediments from the longer core are dominated by clastic matter, which was likely deposited during deglaciation of the lake basin. These clastic sediments are overlain by gyttja, which is also present throughout the shorter core. AMS radiocarbon dating was conducted on plant macrofossils of 11 samples from the gyttja in both cores. A reliable chronology was established for both cores, which dated the onset of organic accumulation at 9,500 cal. year BP. The Holocene temperature development, with an early to mid Holocene thermal maximum, is best reflected in the grain-size composition. Nutrient availability was apparently low during the early Holocene and led to low productivity in the lake and its vicinity. From ca. 7,000 cal. year BP, productivity in the lake increased significantly, probably induced by external nutrient input from goose excrements. From this time, micro- and macrofossil remains reflect relatively well the climate history of East Greenland, with a cooling during the middle Holocene, the medieval warming, and the Little Ice Age. The amount of organic matter in the sequence seems to be more affected by lake ice cover or by nutrient supply from the catchment than by temperature changes. The record from Hjort Sø thus reveals the difficulties in interpreting sedimentary records from high arctic regions.

Palynology on lake sediments from the Pyrenees

A multi-proxy palaeoecological investigation including pollen, plant macrofossil, radiocarbon and sedimentological analyses, was performed on a small mountain lake in the Eastern Pyrenees. This has allowed the reconstruction of: (1) the vegetation history of the area based on five pollen diagrams and eight AMS14C dates and (2) the past lake-level changes, based on plant macrofossil, lithological and pollen analysis of two stratigraphical transects correlated by pollen analysis. The palaeolake may have appeared before the Younger Dryas; the lake-level was low and the vegetation dominated by cold steppic grasslands. The lake-level rose to its highest level during the Holocene in the Middle Atlantic (at ca. 5060±45 b.p.). Postglacial forests (Quercetum mixtum and Abieto-Fagetum) developed progressively in the lower part of the valley, while dense Pinus uncinata forests rapidly invaded the surroundings of the mire and remained the dominant local vegetation until present. The observed lowering of the lake levels during the Late Atlantic and the Subboreal (from 5060 ± B.P. to 3590±40 b.p.) was related to the overgrowth of the mire. The first obvious indications of anthropogenic disturbances of the vegetation are recorded at the Atlantic/Subboreal boundary as a reduction in the forest component, which has accelerated during the last two millennia.

On increasing the effective aperture of antennas by data processing /

Includes bibliographical references (p. 58-59)

Proceedings, Second International Conference on Fixed-Film Biological Processes, July 10-12, 1984, Arlington, Virginia /

"EPA/600/9-85/024a."

"Chip's" dogs : a collection of humorous drawings /

Mode of access: Internet.

TDP : a two-dimensional perturbation calculation code based on TDC, for IBM-7090, Deck B 516 /

Work performed at CANEL.