X rays and crystal structure /

Mode of access: Internet.

Addendum to Strategic planning study for flood control : Crystal Creek, Villages of Franklin Park & Schiller Park, Cook County, Illinois.

"OWR/SPS/99-003."

Strategic planning study for flood control : Crystal Creek, Villages of Franklin Park & Schiller Park, Illinois, Cook County.

"DOWR/SPS/91-001."

Crystal growth.

Includes bibliographies.

Crystal data; determinative tables.

Errata pages inserted in vol. 2.

The closest packing of spheres : a unifying basis for crystal structures /

"AES research and development report"--Cover.

Train wreck Crystal Falls, Mich.

verso: this is a wreack [sic] we had some time ago, were [sic] you see the cross, the engineer was pined [sic[ two feet in the ground you can imagine what he looked lik [sic]

Crystal structures of fusion proteins with large-affinity tags

The fusion of a protein of interest to a large-affinity tag, such as the maltose-binding protein (MBP), thioredoxin (TRX), or glutathione-S-transferase (GST), can be advantageous in terms of increased expression, enhanced solubility, protection from proteolysis, improved folding, and protein purification via affinity chromatography. Unfortunately, crystal growth is hindered by the conformational heterogeneity induced by the fusion tag, requiring that the tag is removed by a potentially problematic cleavage step. The first three crystal structures of fusion proteins with large-affinity tags have been reported recently. All three structures used a novel strategy to rigidly fuse the protein of interest to MBP via a short three- to five-amino acid spacer. This strategy has the potential to aid structure determination of proteins that present particular experimental challenges and are not conducive to more conventional crystallization strategies (e.g., membrane proteins). Structural genomics initiatives may also benefit from this approach as a way to crystallize problematic proteins of significant interest.