Investigation on the s of guava (Psidium guajava L.) infusions on germination, root tips and meristematic cells of Latuca sativa

Guava (Psidium guajava L.) is a plant often employed in popular medicine. Recently several studies have alerted about the toxicity of substances present in medicinal plants, which can pose risks to the human health. In this sense, the present work aimed to investigate the phytotoxic, cytotoxic and genotoxic action of three guava varieties - Paluma, Pedro Sato and Roxa (purple) - on the plant test system Lactuca sativa L. Thus, macro- and microscopic evaluations were carried out for five infusion concentrations (2.5, 5.0, 10.0, 20.0 and 40.0 g.L-1) prepared from each variety. Distilled water was used as negative control. Chromatographic and spectroscopic analysis by HPLC-PAD indicated that the chemical composition of the infusion of Roxa is different than that of the infusions of the varieties Paluma and Pedro Sato. It was observed that seed germination and root growth in L. sativa exposed to infusions decreased with increasing infusion concentration, regardless of the tested cultivar. For the mitotic index, no statistical differences were observed. On the other hand, a significant increase in the frequency of cell cycle alterations was verified, especially for the highest concentrations tested. The cytogenotoxic was significant. Therefore, guava should not be used indiscriminately in popular medicine.

Effects of hormonal priming on seed germination of pigeon pea under cadmium stress

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Subdoses de glyphosate no desenvolvimento de espécies arbóreas nativas

Recovery of degraded areas aims to provide the degraded environment favorable conditions for restructuring in an environment that is unable to regenerate itself and planting of tree seedlings is one of the effective ways to extend this process. However, native tree species have slow growth rate which increases the seedlings production time as well as decreases their competitiveness with weeds in areas of forests deployment. The aim of this study was to evaluate the effects of glyphosate reduced rates application on Psidium cattleyanum (yellow guava), Citharexylum myrianthum (pau-viola) and Cedrela odorata (cedro) seedlings development by analyzing its morphophysiological parameters. The experiment was carried out in greenhouse conditions and the experimental units consisted of one plant were conducted in 2.5L plastic pots and arranged in a completely randomized design with six replications. Treatments consisted of glyphosate application at 0, 7.5, 15, 30 and 60 g ha(-1) using Scout (R) commercial formulation. Treatments application on these species seedlings was realized 120 days after seeds germination. Visual evaluations of plants phytotoxicity were realized at 7, 14 and 21 days after treatments application and plants survival, stomatal conductance, height, stem diameter and dry mass of shoots were evaluated 90 days after treatments application. Different doses of glyphosate did not cause plants phytotoxicity. Already, 30 and 60 g ha(-1) of glyphosate provided greater increases in height, stem diameter and dry mass of shoots for Citharexylum myrianthum and Psidium cattleyanum, respectively.

Loss of desiccation tolerance in Copaifera langsdorffii Desf. seeds during germination

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A virtual experiment for the detection of specific features in the frequency response of a coupled nonlinear and linear oscillator

This paper presents an investigation into some practical issues that may be present in a real experiment, when trying to validate the theoretical frequency response curve of a two degree-of-freedom nonlinear system consisting of coupled linear and nonlinear oscillators. Some specific features, such as detached resonance curves, have been theoretically predicted in multi degree-of-freedom nonlinear oscillators, when subject to harmonic excitation, and the system parameters have been shown to be fundamental in achieving such features. When based on a simplified model, approximate analytical expression for the frequency response curves may be derived, which may be validated by the numerical solutions. In a real experiment, however, the practical achievability of such features was previously shown to be greatly affected by small disturbances induced by gravity and inertia, which led to some solutions becoming unstable which had been predicted to be stable. In this work a practical system configuration is proposed where such effects are reduced so that the previous limitations are overcome. A virtual experiment is carried out where a detailed multi-body model of the oscillator is assembled and the effects on the system response are investigated.

Improvement to the physical quality and imbibition pattern in seeds of candeia (Eremanthus incanus (Less.) Less.)

The objective of this work was to develop a methodology for seed processing and X-ray analysis and to study imbibition pattern in seeds of candeia (Eremanthus incanus). Seeds were gathered in 2001 and 2002 in Morro do Pilar and Lavras respectively, processed and stored in a cold chamber (5ºC/60%RH) until the start of experiments. In order to identify unfilled seeds, a protocol was developed for X-ray use combining radiation energy (Kv) and exposure time (seconds). For elimination of unfilled seeds, an experiment was conducted using a South Dakota seed blower whereby different opening settings and ventilation times were combined. Original seed lots and categorized lots following radiograph viewing were tested by germination tests, with seeds being cleansed in sodium hypochlorite and scattered over blotting paper in 'gerboxes' and then taken to germinators with alternating temperatures of 20º-30ºC and 10 hours of light, for up to 15 days. Imbibition curves were determined under the conditions 30ºC, 20º-30ºC/10 hours of light and 30º-20ºC/10 hours of light. The X-ray protocol that best allowed viewing of internal seed structures was 30Kv for 45 seconds. Seed separation using the blower setting at a 3.0 opening for 30 seconds raised the number of embryonic seeds to values exceeding 99%, also raising the germination rates as a consequence. The imbibition pattern is three-phased and the germination process is completed in 72 hours. Under alternate temperatures, primary root growth is favored.

Efeito de extratos de adubos verdes sobre Lactuca sativa e Digitaria horizontalis

Objetivou-se avaliar os efeitos de extratos de quatro espécies de adubos verdes (Canavalia ensiformis, Crotalaria spectabilis, Mucuna aterrimae Sorghum bicolor cv. AG 2005) sobre a germinação de sementes e o crescimento inicial de plântulas deLactuca sativa e de Digitaria horizontalis. Foram realizados dois experimentos: no primeiro, avaliou-se a aplicação das soluções diluídas a 1%, 2%, 3%, 4%, 5% e10% de extratos aquosos da parte aérea e das raízes das espécies de adubos verdes sobre a germinação, índice de velocidade de germinação e o comprimento radicular de L. sativa; no segundo, avaliou-se a incorporação ao solo de 0, 10, 20, 30, 40 e 50 g dm-3 da biomassa dos adubos verdes sobre o acúmulo de massa seca (parte aérea e raízes) e o desenvolvimento radicular de plântulas de L. sativa e de D. horizontalis. Concluiu-se que os extratos aquosos das espécies C. ensiformis, C. spectabilis, M. aterrima e S. bicolor cv. AG 2005 promoveram efeitos negativos sobre a germinação e o IVG das sementes de L. sativa. A incorporação ao solo da biomassa dessas espécies de adubos verdes influenciou negativamente no desenvolvimento inicial das plântulas de L. sativa. Entretanto, verificou-se efeito positivo sobre o desenvolvimento inicial de D. horizontalis com a incorporação ao solo da biomassa de C. spectabilis.

Balanço econômico da operação de semeadura da cultura da soja em diferentes arranjos espaciais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Micronutrientes e biorregulador via foliar em diferentes estádios de desenvolvimento de cultivares de feijão, visando produção e qualidade de sementes

Pós-graduação em Agronomia - FEIS

Efeitos de produtos alternativos no controle de doenças na videira

Pós-graduação em Agronomia - FEIS

Temperatura, luz e tolerância à dessecção na germinação de sementes de açaí-do-Amazonas

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Permeabilidade a água e germinação de sementes heteromórficas de Bowdichia virgilioides Kunth

Pós-graduação em Ciência Florestal - FCA

Allelopathic potential of Equisetum giganteum L. and Nephrolepis exaltata L. on germination and growth of cucumber and lettuce

Nephrolepis exaltata L. Schott "Bostoniensis" family Davalliaceae and Equisetum giganteum L. family Equisetaceae, Phylum Pteridophyta, exhibit a strong mechanism of dominance in the areas in which they live. Have secondary compounds with allelopathic activity. The objective of this article was evaluate allelopathic potential of two ferns species, using bioassay applying aqueous extracts of dried fronds, in cucumber and lettuce seeds, and observing germination and initial development. To observe the influence on germination was analyze the percentage of germinated seeds and germination speed index (GSI). To observe initial development was analyzed shoot and root growth of the seedlings. The bioassays revealed that no concentration significantly inhibited the germination, but germination speed was delayed gradually in two species tested, as increased the extract concentration. In initial development, all the extracts showed a tendency to inhibit the growth, and an increase in extract concentration decreasing growth of radicle and hypocotyl axis. We conclude that the aqueous extract has inhibitory activity more pronounced in early development than in seed germination, affecting the primary structures of the tested plants, corroborating with the observations of occurrences of the species in natural places where dominate and suppress the growth of other species.

Vibração de plantas de pimenta Malagueta para produção de frutos e sementes em ambiente protegido

This study aimed to verify the effect of vibration of plants in the production of fruits and seeds and in the physiological quality of the seeds of the “Malagueta” pepper in a protected cultivated area with open and closed sides. The experiment was conducted at the Experimental Farm of the School of Agronomic Sciences of Universidade Estadual Paulista (FCA/UNESP), located in the city of São Manuel (SP). Two treatments were carried out, which consisted of plants that did and did not undergo vibration. Plants were vibrated manually by swinging the wire attached to the supporting bamboo for about 5 seconds, twice a day for 11 weeks. The design was a randomized block with six repetitions. The same experiment was conducted in two protected environments, with and without anti-aphids screens on the sides, cultivated in the same season. For the comparison of environments, a combined analysis was performed considering each environment as a separate experiment. The characteristics evaluated were: weight and number of fruits per plant; weight, diameter and length of fruit; weight and amount of seeds per fruit; and plant height. For the analysis of seed quality, the characteristics evaluated were: germination percentage; first count of germination; dry matter of seedlings; seedling emergence in substrate; and seedling emergence speed index. Regardless of the environment, vibration affects the physiological quality of seeds. The presence of pollinator insects benefited the production and quality of “Malagueta” pepper seeds and increases the fruit weight.