Atividade citotóxica e apoptótica de nitensidina A e B em células de carcinoma cervical imortalizadas pelo Papiloma Vírus Humano (HPV) e não infectadas

Câncer cervical é o segundo tipo mais comum em mulheres no mundo. Estudos apontam que a presença de certos tipos do HPV desempenha um papel central na formação do câncer. Apesar do grande sucesso da prevenção do câncer uterino com vacinas, o uso de produtos naturais para o tratamento de doenças é crescente e de grande importância econômica. Alcalóides guanidínicos isolados de Pterogyne nitens representam uma potencial fonte de novos tratamentos. O objetivo geral desse trabalho foi avaliar a atividade apoptótica induzida em células de carcinoma cervical infectadas (SiHa) e não infectadas pelo HPV (C33-A), tratadas com nitensidinas A e B, isoladas de folhas de Pterogyne nitens Tul. A citotoxicidade dos alcalóides foi avaliada utilizando o método de MTT. Para avaliar quantitativamente a apoptose e necrose celular, foi executado o ensaio de Anexina V por citometria de fluxo. Ambas as substâncias, nitensidinas A e B, apresentaram efeito citotóxico concentração-resposta em ambas as linhagens testadas (SiHa e C33A) e nos dois tempos de tratamento (24 horas e 48 horas). Não foi observada significância estatística quando comparados os perfis de ação citotóxica das duas substâncias em teste (nitensidinas A e B), em nenhuma das linhagens testadas (SiHa e C33A) e em nenhum dos dois tempos de tratamento (24 horas e 48 horas). Ambas as substâncias, nitensidina A e nitensidina B, apresentaram efeito indutor de apoptose precoce e, em menor número, de apoptose tardia/ necrose, em ambas as linhagens testadas (SiHa e C33A) e nos dois tempos de tratamento (24 horas e 48 horas)

Estudo in vitro da anatomia das projeções de esmalte cervical em dentes molares

The cervical enamel projection (CEP) is an anatomic variation that can be found in the cement enamel junction, it´s recognition and early diagnosis improves outcome the tooth involved, allowing greater efficiency in treatment. The knowledge of the location, grade and incidence of CEP is decisive on prevention and treatment of periodontal disease. It were studied 1200 molars (600 mandibular molars and 600 maxillary molars) separated from the collection of teeth on the Discipline of Anatomy in FOSJCampos - UNESP. Each group of 600 teeth was separated from the second side (300 teeth) and the second type (first, second or third molar). Each tooth had thoroughly inspected the cervical region, in all their faces, in order to verify the incidence and the grade of CEP in each surface. It was found that the CEP covered in 278 (23,17%) teeth was 146 (52,52%) mandibular molars and 132 (47,48%) maxillary molars. The CEPs were concentrated on a tooth surface in 222 (79,86%) teeth, in two surfaces 53 (19,06%) teeth, and in three surfaces in three (1,08%) teeth. From the total of 4.800 surfaces examined the CEPs were found in 337 surfaces, and 228 (67,66%) surfaces of the grade I, 60 (17,8%) grade II and 49 (14,54%) grade III. The buccal surface presented 207 (61,42%) CEP, the lingual surface in 57 (16,91%), the mesial surface in 35 (10,39%) and the distal surface in 38 (11,28%) CEP. The results of this study demonstrated that the CEPs have focused more on the mandibular teeth on only one surface of the tooth, the grade I was the most found and the buccal surface the most involved

Avaliação de concentração de citocinas no soro de pacientes portadoras de lesão intraepitelial escamosa de baixo grau, lesão intraepitelial escamosa de alto grau e carcinoma cervical invasor

A infecção genital por Papilomavírus Humano (HPV) é uma das doenças sexualmente transmissíveis (DST), de origem viral, mais prevalente no mundo. As lesões intraepiteliais escamosas de baixo grau (LIEBG) e de alto grau (LIEAG), assim como carcinoma cervical invasor (CCI), estão associadas à presença do HPV. A resposta imune tem papel importante na infecção pelo HPV na cérvice uterina, sendo as citocinas importantes reguladoras da transcrição viral. Avaliar a concentração de IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IFN-γ e TNF-α no soro de mulheres portadoras de LIEBG, LIEAG e CCI. Foram incluídas no estudo 40 mulheres com diagnóstico histopatológico de LIEBG (n=11), LIEAG (n=10), CCI (n=10) e 9 mulheres com suspeita de doença HPV induzida, mas sem alterações histopatológicas na biópsia do colo do útero (controle), atendidas no Ambulatório de Colposcopia da Faculdade de Medicina de Botucatu (UNESP) e no Ambulatório de Ginecologia Preventiva do Hospital Amaral Carvalho, Jaú, SP. O sangue periférico foi colhido por punção venosa e o soro armazenado até o processamento. A dosagem das citocinas no soro foi avaliada por ensaio imunoenzimático (ELISA). A pesquisa de HPV foi realizada empregando-se a técnica da reação em cadeia da polimerase (PCR). Os dados sócio-demográficos foram obtidos por entrevista no momento da consulta ginecológica. A mediana de idade das pacientes do grupo controle, LIEBG, LIEAG, e CCI foi de 38 (21-69), 32,5 (17-51), 39 (23-65) e 51,5 (29-72), respectivamente. Houve diferença estatisticamente significante de mediana de idade das pacientes do grupo CCI quando esta foi comparada com os demais grupos. Em relação às características das pacientes, 76% eram brancas, 68% relataram união estável, 31% concluíram o 2º grau, 42% eram fumantes, 37% das mulheres relataram 3 ou mais parceiros sexuais durante... (Resumo completo, clicar acesso eletrônico abaixo)

Correlation Between the Friedman Staging System and the Upper Airway Volume in Patients With Obstructive Sleep Apnea

Purpose: This study was designed to evaluate the correlation between computed tomography findings and data from the physical examination and the Friedman Staging System (FSS) in patients with obstructive sleep apnea (OSA). Patients and Methods: We performed a retrospective evaluation by reviewing the medical records of 33 patients (19 male and 14 female patients) with a mean body mass index of 30.38 kg/m 2 and mean age of 49.35 years. Among these patients, 14 presented with severe OSA, 7 had moderate OSA, 7 had mild OSA, and 5 were healthy. Results: The patients were divided into 2 groups according to the FSS: Group A comprised patients with FSS stage I or II, and group B comprised patients with FSS stage III. By use of the Fisher exact test, a positive relationship between the FSS stage and apnea-hypopnea index ( P = .011) and between the FSS stage and body mass index ( P = .012) was found. There was no correlation between age ( P = .55) and gender ( P = .53) with the FSS stage. The analysis of variance test comparing the upper airway volume between the 2 groups showed P =.018. Conclusions: In this sample the FSS and upper airway volume showed an inverse correlation and were useful in analyzing the mechanisms of airway collapse in patients with OSA.

Hipersensibilidade dentinária cervical: em busca de um tratamento eficaz

The knowledge of the etiology of any disease or condition is paramount to a safe and effective treatment. This literature review aims to show some options to treat dentine hypersensitivity (HSDC). The loss of cervical enamel and cementum exposure of tubules leads to a painful condition and patient discomfort, called HSDC. This loss of tooth structure occurs due to formation of cervical lesions in cases of gingival recession, abrasion, erosion, or abfraction by the association of two or more factors. Some treatments are not effective, but there are effective therapies, such as: application of ferric oxalate, potassium oxalate, potassium nitrate, fluoride varnish, solutions of calcium phosphate, adhesives and Bonding procedures. Therefore, the identification and removal of etiological factors is essential to successful treatment of HSDC normally associated to tubules obliterate and consequent reduction of fluid motion within the dentin.

Correlación entre diferentes métodos de evaluación de color en el clareamiento dental. Correlation between different methods for co

The aim of this study was to evaluate the correlation of color change on bleaching tooth through delta E (ΔE) by the spectrophotometer Spectroshade (SS) and digital program Scanwhite (SW). Methods: 25 patient volunteers were recruited from Operative Dentistry at Universidad de Chile Dental School, between 18 to 30 years, with good oral hygiene. Exclusion criteria: Previous experienced tooth bleaching, anterior restorations, cervical lesions, pain dental, pregnant patient, stained teeth, malposition of the teeth and periodontal pathology. The bleaching was made in two sessions with three different bleaching systems which were randomly. The assessment of color change through ΔE was made on the two upper central incisors (N=50) by the SS and SW. The color in the same teeth were measured before (T0) and after (T1) of the bleaching treatment. Data were analyzed using test Spearman correlation coefficient (Rho) with a significant level of 95%. Results: The results showed a positive and significant correlation (0.676), with a statistically significant difference (p<0.05). Conclusion: There is correlation in the measurement of color change on bleaching tooth by SS and SW.

Influence of spacing in the upper lateral incisor area on the perception of smile esthetics among orthodontists and laypersons

Background: The aim of this study was to determine the perception of smile esthetics among orthodontists and laypeople with respect to the presence of diastemas in the upper lateral incisor in the mesial, distal, and both surfaces using an oblique smile analysis. Methods: Two standardized oblique photos of pleasant smiles from two white women were selected. Images were digitally altered to create diastemas in the lateral incisor, in 0.5-mm increments, in the mesial, distal, or both surfaces. Final images were randomly assembled in a photo album, which was given to 120 judgesd60 orthodontists and 60 laypersons. Each rater was asked to evaluate the attractiveness of the images on a visual analog scale. The data collected were submitted to statistical analysis by the means of one-way ANOVA with the Tukey post hoc test and the unpaired Student’s t test. Results: The most attractive smile was the one without spacing, and the presence of diastemas was considered unattractive by both groups of raters, following a pattern: the greater and the more mesially located, the more unattractive was the smile. Conclusions: The results of this study suggest that diastemas in the upper lateral incisor area were considered unattractive, following a pattern: the greater and the more mesially located, the more unattractive was the smile.

A method for retrieving endodontic or atypical non-endodontic separated instruments from the root canal: a report of two cases

Aim: This clinical report presents a new method for retrieving separated instruments from the root canal with minimally invasive procedures. Background: The presence of separated instrument in root canal may interfere in the endodontic treatment prognosis. There are several recommended methods to retrieve separated instruments, but some are difficult in clinically practice. Case report: This study describes two cases of separated instrument removal from the root canal using a stainless-steel prepared needle associated with a K-file. Case 1 presented a fractured gutta-percha condenser within the mandibular second premolar, it was separated during incorrect intracanal medication calcium hydroxide placement. Case 2 had a fractured sewing needle within the upper central incisor that the patient used to remove food debris from the root canal. After cervical preparation, the fractured instruments were fitted inside a prepared needle and then an endodontic instrument (#25 K-file) was adapted with clockwise turning motion between the needle inner wall and the fragment. Conclusion: The endodontic or atypical nonendodontic separated instrument may be easily pull on of the root canal using a single and low cost device. Clinical significance: The methods for retrieving separated instruments from root canal are difficult and destructive procedures. The present case describes a simple method to solve this problem.

Musculoskeletal disorders in upper limbs in dental students: exposure level to risk factors

Aim: This study assessed the risk factors of undergraduate students to develop musculoskeletal disorders (MSD) in the upper limbs, regarding gender, type of dental clinical procedure, mouth region treated, and the four-handed dentistry practice. Methods: Dental students enrolled in the 8th semester in the Araraquara School of Dentistry, UNESP, Brazil, were photographed while practicing 283 dental procedures. The Rapid Upper Limb Assessment (RULA) method was used to evaluate the working postures of each student. The photographs were evaluated and a final risk score was attributed to each analyzed procedure. The prevalence of risk factors of developing MSD was estimated by point and by 95% confidence interval. The association between the risk factor of developing disorders and variables of interest were assessed by the chi-square test with a significance level of 5%. Results: The risk factors of developing MSD were high, regarding most dental procedures performed by the undergraduate students (score 5: 7.07%, CI95%: 4.08- 10.06%; score 6: 62.54%, CI95%: 56.90-68.18%). There was no significant association between the RULA final score and gender (p=0.559), and type of dental procedure (p=0.205), and mouth regions by arch (p=0.110) or hemi-arch (p=0.560), and the use of four-handed dentistry (p=0.366). Conclusions: It can be concluded that gender, type of dental clinical procedure, mouth region treated, and practice of four-handed dentistry did not influence the risk of developing MSD in the upper limbs among the dental students evaluated; however, they are at a high risk of developing such disorders.

Risk muskuloskeletal disorders in upper limbs in dental students: concordance of different methods for estimation of body angle

The purpose of this paper was to evaluate the agreement among different methods used to estimate angular deviation of the body to determine the risk for development of upper limb musculoskeletal disorders in dentistry undergraduates. Materials and Methods: Students (n = 79) enrolled in the final year undergraduate course of the Araraquara School of Dentistry-Sγo Paulo State University-UNESP were evaluated. Photographs were taken of students performing clinical procedures. The work postures adopted by each student were evaluated by means of rapid upper limb assessment (RULA). The basis used to obtain the individual's final risk score is the measurement of the angular deviations in the neutral positions of the regions evaluated. Two methods were used to estimate the angular deviation of the body: Visual exam and Image Tool software. A RULA final risk score was attributed to each procedure the student performed (n = 333). Study of the agreement between the methods about risk of musculoskeletal disorders was conducted by means of Kappa (κ) statistics. The level of significance adopted was 5%. Results: Fair agreement (κ = 0.32) between the evaluated methods was verified. Conclusion: The risk for development of upper limb musculoskeletal disorders by dentistry undergraduates evaluated by using RULA was not in agreement with the results obtained by use of visual exam and Image Tool.

Risk of musculoskeletal disorders in upper limbs in dental students: concordance of different methods for estimation of body angle

The purpose of this paper was to evaluate the agreement among different methods used to estimate angular deviation of the body to determine the risk for development of upper limb musculoskeletal disorders in dentistry undergraduates. Materials and Methods: Students (n = 79) enrolled in the final year undergraduate course of the Araraquara School of Dentistry-Sγo Paulo State University-UNESP were evaluated. Photographs were taken of students performing clinical procedures. The work postures adopted by each student were evaluated by means of rapid upper limb assessment (RULA). The basis used to obtain the individual's final risk score is the measurement of the angular deviations in the neutral positions of the regions evaluated. Two methods were used to estimate the angular deviation of the body: Visual exam and Image Tool software. A RULA final risk score was attributed to each procedure the student performed (n = 333). Study of the agreement between the methods about risk of musculoskeletal disorders was conducted by means of Kappa (κ) statistics. The level of significance adopted was 5%. Results: Fair agreement (κ = 0.32) between the evaluated methods was verified. Conclusion: The risk for development of upper limb musculoskeletal disorders by dentistry undergraduates evaluated by using RULA was not in agreement with the results obtained by use of visual exam and Image Tool.

Análise dos troncos radiculares, abertura da raízes e projeções cervicais de esmalte de furcas de molares superiores e inferiores: Estudo in vitro

Periodontal treatment of teeth with furcation lesions often have unpredictable results due to several factors that may hinder therapy or influence the progress of periodontal disease. Of these, the length of the root trunk may contribute to the early injury of the furcations or even cause complications for the application of the treatment; the opening of the roots can guide the type of treatment to be instituted and cervical enamel projections may hinder the instrumentation of the region or even act as a retention plate niche. Thus, the purpose of this study was measured using a digital caliper, root logs database 400 molar both the 1st and the 2nd and both upper and lower as well as analyze using appropriate index type projection cervical enamel and through appropriate classification the opening of the roots in order to guide the diagnosis and treatment plan. We conclude that there is variation in measures of root trunk between the faces of the teeth examined and that greater cervical enamel projection (ECP) correlates with a decrease in the length of the root trunk in the sample evaluated as the first molars have higher occurrence of roots with a degree of opening larger than the second molars.

Recobrimento radicular para o tratamento de hipersensibilidade dentinária cervical persistente

Gingival recession has a high prevalence in the population. This alteration may promote several complications such as cervical dentinal hypersensitivity (HSDC), carious and non-carious cervical lesions, and cosmetic changes due to the increase of the clinical crown length. To treat HSDC there are several therapeutic possibilities that should aim to eliminate pain after external stimulus. Treatment of this alteration can be performed by a conventional or invasive procedure, depending on the clinical condition and patients' desires. In situations where aesthetics is not the main complaint and HSDC persists even after conservative procedures, it is possible to indicate a procedure to achieve root coverage in Miller's Class I and II recessions. The aim of this paper is to report a case where the HSDC was not eliminated by conventional methods, which was resolved only after performing a minimally invasive surgery that promoted total root coverage.

Dentinogenesis imperfecta type II: approach for dental treatment

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Pharyngeal airway space cephalometric evaluation in transverse maxillary deficient patients after SARME

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)