993 resultados para Sistemas de Tempo Real


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Pós-graduação em Doenças Tropicais - FMB

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Pós-graduação em Biologia Geral e Aplicada - IBB

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Pós-graduação em Medicina Veterinária - FMVZ

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Pós-graduação em Biopatologia Bucal - ICT

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Pós-graduação em Microbiologia - IBILCE

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Luís Guilherme Coelho Buchianeri propõe, nesta obra, que nos dias atuais a velocidade continua acelerando exponencial e paradoxalmente, mas apenas no mundo externo. No mundo interno, se desacelera, “tendendo a uma paralisia que não promove a angústia estruturante, mas leva à agonia, à sensação de falta de futuro, à necessidade de preenchimento do tempo com conteúdos dados à imediaticidade dos afetos”. Tal busca de concretude, diz, impede a subjetivação do ser humano moderno. A subjetividade, segundo o autor, considerada lenta e imprecisa, está sendo descartada na era da instantaneidade e da satisfação imediata dos desejos, em que a máquina possibilita que tudo se realize em “tempo real”. Assim, se no nascimento da modernidade a velocidade instigava a ação, transformação e rebeldia, hoje, vazia de conteúdos, paralisa e instala nesse espaço um tempo entediante. Nos jovens, principalmente, essa variedade de estímulos decorrentes dessas mutações no espaço e no tempo produziria uma falta de sentido na vida, acompanhada por “um esvaziamento do sujeito, uma sensação subjetiva a que poderíamos denominar tédio”. Paralelamente a esse tema, discutido amplamente neste livro, que leva em conta a realidade de jovens de diversos países, o autor aborda outra questão contemporânea – a da “depressão como figura de subjetivação da atualidade”. Ao analisar conceitos com base em teorias da Psicologia e da Filosofia, ele procura demonstrar que o tédio se sobressai em relação à depressão como a subjetividade típica de um mundo acelerado e volátil

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Chlamydophila psittaci is a bacterium that causes respiratory or systemic disease in birds and humans. Owing to the risk of transmission from asymptomatic birds to humans, the objective of this study was to detect the presence of Chlamydophila spp. in asymptomatic birds. Four hundred and three fecal samples or cloacal swabs were collected from domestic, wild or exotic birds. The 403 samples were examined by real time PCR specific for the 16S subunit of rRNA gene using SsoFastEvaGreen®SupermixTM (Bio-Rad) and melting curve analysis. Hemi-nested PCR specific for the OMP-A gene, accomplished in real-time PCR positive samples, was followed by sequencing of the amplified fragments to determine the genotype of C. psittaci. Real-time PCR was positive in 17 (4.21%) samples. Hemi-nested PCR revealed positivity in two samples previously positive by real-time PCR. Sequencing of the fragment amplified by hemi-nested PCR allowed for the identification of genotype A of C. psittaci in one sample. The results of this experiment show that the real-time PCR targeting the 16S rRNA gene followed by melting curve analysis can be used for diagnosis of Chlamydophila sp. in fecal samples of asymptomatic birds. The classification of the Chlamydophila species and the genotype of C. psittaci must be accomplished by PCR targeting the ompA gene and sequencing of the amplified fragments.

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Skeletal muscle growth in the pirarucu (Arapaima gigas) is highly interesting to fish farmers because it provides information about how the mechanism in muscle mass increase, characteristic of the species, is regulated. Pirarucu has specific muscle growth that highlights the species's significance and commercial value. Current research evaluates the morphology and the growth-related gene expression in the red and white skeletal muscles of the pirarucu. Muscle samples were collected from the lateral anterior region and frozen in liquid nitrogen. Histological sections were performed and stained by HE for morphological analysis. Red and white muscle samples were used to determine MyoD, myogenin, and myostatin genes expression by Real-time Polymerase Chain Reaction. Although MyoD and myogenin were not statistically different in the two types of muscles, myostatin was significantly higher in the white rather than in the red muscle. Results show the muscle growth characteristics of the species and may be helpful for improving aquaculture management programs.

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In the past few years several GPS (Global Position System) positioning techniques have been develope and/or improved with the goal of obtaining high accuracy and productivity in real time. The reference station network concept besides to enabling quality and reliability in positioning for scientific and civil GPS community, allows studies concerning tropospheric refraction modeling in the network region. Moreover, among the network corrections transmission methods available to users, there is the VRS (Virtual Reference Station) concept. In this method, the data of a virtual station are generated near the rover receiver (user). This provides a short baseline and the user has the possibility of using a single frequency receiver to accomplish the relative positioning. In this paper, the methodology applied to generate VRS data, using different tropospheric models is described. Thus, comparative tests were conducted in the four seasons with the NWP/INPE (Numerical Weather Prediction/National Institute for Space Research) and Hopfield tropospheric models. In order to analyse the VRS data quality, it was used the Precise Point Positioning (PPP) method, where satisfactory results were found. Mean differences between PNT/INPE and Hopfield models of 9.75% and 24.2% for the hydrostatic and wet days, respectively were obtained.

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The main topic of this paper is the Ambiguity Dilution of Precision known as ADOP. Basically, ADOP is defined as a diagnostic measure for assessing the precision of the float scalar ambiguities. Among the several possibilities, the ADOP can provide help in predicting the behavior of a baseline or a network of GNSS receivers, concerning the problem of ambiguity resolution, either in real-time (instantaneous) or in the post-processing mode. The main advantage of using ADOP is possibility of the extraction of a closed analytical expression, considering various factors that affect the ambiguity resolution. Furthermore, the ADOP is related to the success rate of ambiguity resolution. The expressions here used, takes into account several factors, for example, a priori information of the measurement precision of GNSS carrier phase and pseudorange, the number of stations and satellites, the number of available frequencies and the behavior of the atmosphere (ionosphere and troposphere). Several scenarios were established so as to analyze the impact of each factor in ambiguities resolution, within the context of some stations of the São Paulo GNSS network (GNSS-SP).

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Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)