Zebrafish eaf1 and eaf2/u19 Mediate Effective Convergence and Extension Movements through the Maintenance of wnt11 and wnt5 Expression

Studies have attributed several functions to the Eaf family, including tumor suppression and eye development. Given the potential association between cancer and development, we set forth to explore Eaf1 and Eaf2/U19 activity in vertebrate embryogenesis, using zebrafish. In situ hybridization revealed similar eaf1 and eaf2/u19 expression patterns. Morpholino-mediated knockdown of either eaf1 or eaf2/u19 expression produced similar morphological changes that could be reversed by ectopic expression of target or reciprocal-target mRNA. However, combination of Eaf1 and Eaf2/U19 (Eafs)-morpholinos increased the severity of defects, suggesting that Eaf1 and Eaf2/U19 only share some functional redundancy. The Eafs knockdown phenotype resembled that of embryos with defects in convergence and extension movements. Indeed, knockdown caused expression pattern changes for convergence and extension movement markers, whereas cell tracing experiments using kaeda mRNA showed a correlation between Eafs knockdown and cell migration defects. Cardiac and pancreatic differentiation markers revealed that Eafs knockdown also disrupted midline convergence of heart and pancreatic organ precursors. Noncanonical Wnt signaling plays a key role in both convergence and extension movements and midline convergence of organ precursors. We found that Eaf1 and Eaf2/U19 maintained expression levels of wnt11 and wnt5. Moreover, wnt11 or wnt5 mRNA partially rescued the convergence and extension movement defects occurring in eafs morphants. Wnt11 and Wnt5 converge on rhoA, so not surprisingly, rhoA mRNA more effectively rescued defects than either wnt11 or wnt5 mRNA alone. However, the ectopic expression of wnt11 and wnt5 did not affect eaf1 and eaf2/u19 expression. These data indicate that eaf1 and eaf2/u19 act upstream of noncanonical Wnt signaling to mediate convergence and extension movements.

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Natl Tech Univ Ukraine, Huazhong Univ Sci & Technol, Huazhong Normal Univ, Wuhan Univ, Ternopil Natl Econ Univ, IEEE Ukraine Sect, I&M CI Joint Chapter

延安黄土丘陵沟壑区山坡防蚀道路技术体系及指标

陕北黄土丘陵沟壑区 ,生态环境脆弱 ,水土流失严重 ,作为环境脆弱带的山坡生产型道路 ,对加剧土壤侵蚀起着重要的作用 ,属等外路。人们并不很重视的山坡生产型道路却与农村生产运输、经济交流密切相关 ,其建设与维护具有重要意义。通过对延安黄土丘陵沟壑区山坡生产型道路侵蚀状况调查及对道路防蚀建设的经验 ,总结了现有山坡道路侵蚀现状、方式、影响因素 ,并从生物、工程两方面初步阐述防蚀措施的配置与设计