Cultura escrita em contextos indígenas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Relações de poder em parcerias de teletandem

Pós-graduação em Estudos Linguísticos - IBILCE

Tributação do comércio eletrônico e integração regional

Pós-graduação em Direito - FCHS

A frente nacional contra a privatização da sáude: direito garantido, não se compra, não se vende

Pós-graduação em Serviço Social - FCHS

A ideologia dos meios de comunicação social na formação da consciência familiar

Pós-graduação em Serviço Social - FCHS

O Serviço Social e a avaliação de impacto na gestão de programas e projetos empresariais

Pós-graduação em Serviço Social - FCHS

Questão jurídica e social para o Terceiro Setor: associações sem fins lucrativos de atividades profissionais e clubes de serviços - Franca-SP

Pós-graduação em Serviço Social - FCHS

A relação família - escola: concepções e práticas

Pós-graduação em Serviço Social - FCHS

Neurotoxicity of Anhydroecgonine Methyl Ester, a Crack Cocaine Pyrolysis Product

Smoking crack cocaine involves the inhalation of cocaine and its pyrolysis product, anhydroecgonine methyl ester (AEME). Although there is evidence that cocaine is neurotoxic, the neurotoxicity of AEME has never been evaluated. AEME seems to have cholinergic agonist properties in the cardiovascular system; however, there are no reports on its effects in the central nervous system. The aim of this study was to investigate the neurotoxicity of AEME and its possible cholinergic effects in rat primary hippocampal cell cultures that were exposed to different concentrations of AEME, cocaine, and a cocaineAEME combination. We also evaluated the involvement of muscarinic cholinergic receptors in the neuronal death induced by these treatments using concomitant incubation of the cells with atropine. Neuronal injury was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. The results of the viability assays showed that AEME is a neurotoxic agent that has greater neurotoxic potential than cocaine after 24 and 48 h of exposure. We also showed that incubation for 48 h with a combination of both compounds in equipotent concentrations had an additive neurotoxic effect. Although both substances decreased cell viability in the MTT assay, only cocaine increased LDH release. Caspase-3 activity was increased after 3 and 6 h of incubation with 1mM cocaine and after 6 h of 0.1 and 1.0mM AEME exposure. Atropine prevented the AEME-induced neurotoxicity, which suggests that muscarinic cholinergic receptors are involved in AEME's effects. In addition, binding experiments confirmed that AEME has an affinity for muscarinic cholinergic receptors. Nevertheless, atropine was not able to prevent the neurotoxicity produced by cocaine and the cocaineAEME combination, suggesting that these treatments activated other neuronal death pathways. Our results suggest a higher risk for neurotoxicity after smoking crack cocaine than after cocaine use alone.

Il sistema di contabilità nazionale: versioni, elementi e regole base

Lucidi utilizzati a lezione e materiali allegati Versione provvisoria del 15 ottobre 2007 Modulo 1°: Lezioni 10-15