Changes in gene expression in PBMCs profiles of PPAR alpha target genes in obese and non-obese individuals during fasting

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Involvement of alpha(1B)-adrenoceptors in the anti-immobility effect of imipramine in the tail suspension test

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Androgen therapy reverses injuries caused by ethanol consumption in the prostate: testosterone as a possible target to ethanol-related disorders

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

An impedimetric biosensor to test neat serum for dengue diagnosis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Thyroid hormone status interferes with estrogen target gene expression in breast cancer samples in menopausal women

We investigated thyroid hormone levels in menopausal BrC patients and verified the action of triiodothyronine on genes regulated by estrogen and by triiodothyronine itself in BrC tissues. We selected 15 postmenopausal BrC patients and a control group of 18 postmenopausal women without BrC. We measured serum TPO-AB, TSH, FT4, and estradiol, before and after surgery, and used immunohistochemistry to examine estrogen and progesterone receptors. BrC primary tissue cultures received the following treatments: ethanol, triiodothyronine, triiodothyronine plus 4-hydroxytamoxifen, 4-hydroxytamoxifen, estrogen, or estrogen plus 4-hydroxytamoxifen. Genes regulated by estrogen (TGFA, TGFB1, and PGR) and by triiodothyronine (TNFRSF9, BMP-6, and THRA) in vitro were evaluated. TSH levels in BrC patients did not differ from those of the control group (1.34 ± 0.60 versus 2.41 ± 1.10  μ U/mL), but FT4 levels of BrC patients were statistically higher than controls (1.78 ± 0.20 versus 0.95 ± 0.16 ng/dL). TGFA was upregulated and downregulated after estrogen and triiodothyronine treatment, respectively. Triiodothyronine increased PGR expression; however 4-hydroxytamoxifen did not block triiodothyronine action on PGR expression. 4-Hydroxytamoxifen, alone or associated with triiodothyronine, modulated gene expression of TNFRSF9, BMP-6, and THRA, similar to triiodothyronine treatment. Thus, our work highlights the importance of thyroid hormone status evaluation and its ability to interfere with estrogen target gene expression in BrC samples in menopausal women.

Maximal power output during incremental cycling test is dependent on the curvature constant of the power-time relationship

The aim of this study was to investigate whether the maximal power output (Pmax) during an incremental test was dependent on the curvature constant (W') of the power-time relationship. Thirty healthy male subjects (maximal oxygen uptake = 3.58 ± 0.40 L·min(-1)) performed a ramp incremental cycling test to determine the maximal oxygen uptake and Pmax, and 4 constant work rate tests to exhaustion to estimate 2 parameters from the modeling of the power-time relationship (i.e., critical power (CP) and W'). Afterwards, the participants were ranked according to their magnitude of W'. The median third was excluded to form a high W' group (HIGH, n = 10), and a low W' group (LOW, n = 10). Maximal oxygen uptake (3.84 ± 0.50 vs. 3.49 ± 0.37 L·min(-1)) and CP (213 ± 22 vs. 200 ± 29 W) were not significantly different between HIGH and LOW, respectively. However, Pmax was significantly greater for the HIGH (337 ± 23 W) than for the LOW (299 ± 40 W). Thus, in physically active individuals with similar aerobic parameters, W' influences the Pmax during incremental testing.

Resistência de união entre um cimento resinoso e uma cerâmica aluminizada submetida a diferentes tratamentos de superfície

Pós-graduação em Odontologia Restauradora - ICT

Análise da modulação autonômica em repouso e em resposta ao Tilt Test ativo em crianças com diabetes tipo 1

Pós-graduação em Fisioterapia - FCT

Ações educativas para o controle de vetores da dengue e leishmaniose visceral

Results of educative actions on the control of vectors for dengue and leishmaniasis were evaluated by using an online semi-present course directed to teachers from Fundamental I Education level in Araçatuba-SP. 40 teachers from municipal schools located on geographical areas of town with higher occurrence of dengue and leishmaniasis cases, attended to the course, which consisted in a conceptual part directed to specific subjects such as vector borne and zoonotic diseases, responsible ownership of pets and health education and a practical phase, directed to execution and application of educative projects in the target schools. Data about the teacher’s specific knowledge, before and after the attendance to the course, were submitted to the Wilcoxon test with a 5% of significant level. The application of practical educative projects was evaluated by multiplicative actions involving target public. Tests showed statistically significant increase in the knowledge about the studied diseases (p<0.0001) after the course, especially on questions related to etiological agent, clinical symptoms on man and animals and prevention. Activities of knowledge multiplication envolved teachers and students which detected the problems and promoted education actions in their neighborhood as stage performing, group cleaning activities and elaboration and distribution of folders during a student pared. In conclusion, educative actions direct to the control of diseases vectors using a semi-present course, provides new knowledge to the teachers, impelling them to motivate their students to adopt vectors control measures and environmental cares and to sensitive the community to collaborate with control diseases.

Aggregatibacter actinomycetemcomitans em pacientes com síndrome de down: influência da condição periodontal e higiene bucal

Patients with Down syndrome have varying degrees of mental retardation, physical and motor, and apparently are more susceptible to infectious diseases. Thus, the present study aimed to evaluate the occurrence of Aggregatibacter actinomycetemcomitans in saliva and subgingival biofilms and above of children and adolescents with Down syndrome and analyze the influence of diet, socioeconomic and cultural factors and periodontal condition. After assessing the socio-economic and behavioral, were collected clinical specimens, which were transported to the laboratory of Microbiology and Immunology FOA-UNESP for detection of microorganisms by molecular method and periodontal conditions were evaluated according to the Periodontal Screening Index and Recording. The control group consisted of individuals without the syndrome. The results were analyzed by chi-square test for proportion analysis of variables with three or more categories, or the Mann-Whitney test. The data analysis of this study showed that the occurrence of A. actinomycetemcomitans ranged from 0.0% to 25.0%, both in the group of patients with Down syndrome, as for the control group and this was not affected by socioeconomic characteristics of the target population, either by oral hygiene standards

Medicinal plants in pregnancy and lactation: perception of the health risk and practical educational group in Araraquara, São Paulo State, Brazil

The use of medicinal plants among pregnant women and lactating is a common practice in diverse countries. However, many medicinal plants are contraindicated during pregnancy and lactating, due to various adverse effects, such as teratogenic, embryotoxic and abortive effects, exposing these women, their fetus and babies to health unknown risks. Thus, the purpose of this commentary, was to analyze the perception about the use of medicinal plants by pregnant women and lactating registered in the "baby on board" NGO, Araraquara, São Paulo state, Brazil, between 2010 at 2013. The group was constituted by 48 women, between the first and last trimester of pregnancy or whilst breastfeeding. Information was collected during group meetings by oral interview, using a questionnaire, as script. The nature of the study was a qualitative analysis. The results were based on reports about the use of medicinal plants by pregnant women during group meetings: use, indication of use, knowledge about risks. All participants received written and oral information about the study and they gave a written informed consent. The use of medicinal plants is a reality among pregnant and lactating women of the "baby on board" NGO. They reported that they feel that "natural" products are not harmful for their health. The primary information sources for the majority of women about medicinal plants during pregnancy are family, neighbors and herbalists. The plants most cited (in popular name in Brazil) were: senna, chamomile, boldo, lemon balm, lemon grass. They were used mainly for: nausea, heartburn, indigestion, flatulence, intestinal and abdominal pain, anxiety, intestinal constipation and low milk production. The pregnant and lactating women lacked knowledge about the health risks of the use of medicinal plants and herbal medicines in pregnancy and lactation. They also reported difficulties in clarifying some questions about the use of medicinal plants with their doctors. The results of the present study showed that educative actions about the rational use of medicinal plants in pregnancy and breastfeeding could be part of the operating protocols to promote the maternal and child health programs in Araraquara. Thus, our results also suggest the importance of creating institutionalized places, to the implementation of continued education programs about rational use of medicinal plants in pregnancy and lactation. These targeted programs are not only for health professionals, but also for community members, pregnant women and breastfeeding. Our results pointed out the importance of guidance of doctors and healthcare professionals on the scientific studies about medicinal plants and herbal medicines and the risk/benefit of using herbs during pregnancy. Finally, it is noted the importance of the health professionals to inform women of childbearing on risks to their health, as well as on possibilities of utilization of herbs during fertile period, giving special attention to the potential risk of self-medication.

Avaliação de danos no material genético em anestesiologistas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Desempenho operacional de transplante manual e mecanizado da cultura da alface

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeitos de instruções preliminares sobre o seguimento de regras

O presente estudo teve como objetivo testar os efeitos de diferentes instruções preliminares sobre o seguimento de regras. Dezoito estudantes universitários foram expostos a um procedimento modificado de escolha de acordo com o modelo e distribuídos em três grupos, que diferiram quanto às instruções preliminares recebidas (completa, sem trechos sobre materiais ou sem trechos sobre conseqüências). Os participantes de cada grupo foram divididos em duas condições experimentais, em que instruções correspondentes ou discrepantes foram apresentadas. Os resultados mostraram que a ausência de trechos sobre materiais dificulta a realização da tarefa e a ausência de trechos sobre as conseqüências diminui a variação de respostas, porém todos os participantes que realizaram corretamente a tarefa seguiram tanto instruções correspondentes quanto discrepantes.

A computer-controlled color vision test for children based on the Cambridge Colour Test

The present study aimed at providing conditions for the assessment of color discrimination in children using a modified version of the Cambridge Colour Test (CCT, Cambridge Research Systems Ltd., Rochester, UK). Since the task of indicating the gap of the Landolt C used in that test proved counterintuitive and/or difficult for young children to understand, we changed the target Stimulus to a patch of color approximately the size of the Landolt C gap (about 7 degrees Of Visual angle at 50 cm from the monitor). The modifications were performed for the CCT Trivector test which measures color discrimination for the protan, deutan and tritan confusion lines. Experiment I Sought to evaluate the correspondence between the CCT and the child-friendly adaptation with adult subjects (n = 29) with normal color vision. Results showed good agreement between the two test versions. Experiment 2 tested the child-friendly software with children 2 to 7 years old (n = 25) using operant training techniques for establishing and maintaining the subjects` performance. Color discrimination thresholds were progressively lower as age increased within the age range tested (2 to 30 years old), and the data-including those obtained for children-fell within the range of thresholds previously obtained for adults with the CCT. The protan and deutan thresholds were consistently lower than tritan thresholds, a pattern repeatedly observed in adults tested with the CCT. The results demonstrate that the test is fit for assessment of color discrimination in young children and may be a useful tool for the establishment of color vision thresholds during development.