Karyotypic conservatism in five species of Prochilodus (Characiformes, Prochilodontidae) disclosed by cytogenetic markers

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Chromosomal organization of repetitive DNA sequences in Astyanax bockmanni (Teleostei, Characiformes): dispersive location, association and co-localization in the genome

Repetitive DNA sequences constitute a great portion of the genome of eukaryotes and are considered key components to comprehend evolutionary mechanisms and karyotypic differentiation. Aiming to contribute to the knowledge of chromosome structure and organization of some repetitive DNA classes in the fish genome, chromosomes of two allopatric populations of Astyanax bockmanni were analyzed using classic cytogenetics techniques and fluorescent in situ hybridization, with probes for ribosomal DNA sequences, histone DNA and transposable elements. These Astyanax populations showed the same diploid number (2n = 50), however with differences in chromosome morphology, distribution of constitutive heterochromatin, and location of 18S rDNA and retroelement Rex3 sites. In contrast, sites for 5S rDNA and H1, H3 and H4 histones showed to be co-located and highly conserved. Our results indicate that dispersion and variability of 18S rDNA and heterochromatin sites are not associated with macro rearrangements in the chromosome structure of these populations. Similarly, distinct evolutionary mechanisms would act upon histone genes and 5S rDNA, contributing to chromosomal association and co-location of these sequences. Data obtained indicate that distinct mechanisms drive the spreading of repetitive DNAs in the genome of A. bockmanni. Also, mobile elements may account for the polymorphism of the major rDNA sites and heterochromatin in this genus. © 2013 Springer Science+Business Media Dordrecht.

Validation of a reference control for an SYBR-Green fluorescence assay-based real-time PCR for detection of bovine herpesvirus 5 in experimentally exposed bovine embryos

The objective of this study was to optimize an internal control to improve SYBR-Green-based qPCR to amplify/detect the BoHV-5 US9 gene in bovine embryos produced invitro and experimentally exposed to the virus. We designed an SYBR-Green-based binding assay that is quick to perform, reliable, easily optimized and compares well with the published assay. Herein we demonstrated its general applicability to detect BoHV-5 US9 gene in bovine embryos produced invitro experimentally exposed to BoHV-5. In order to validate the assay, three different reference genes were tested; and the histone 2a gene was shown to be the most adequate for normalizing the qPCR reaction, by considering melting and standard curves ( p<0.05). On the other hand, no differences were found in the development of bovine embryos invitro whether they were exposed to BoHV-5 reference and field strains comparing to unexposed embryos. The developed qPCR assay may have important field applications as it provides an accurate BoHV-5 US9 gene detection using a proven reference gene and is considerably less expensive than the TaqMan qPCR currently employed in sanitary programs. © 2013 Elsevier Ltd.

Texture profile and correlation between sensory and instrumental analyses on extruded snacks

Instrumental texture analysis on extruded snacks is widely applied, however there is no scientific consensus about the test and probe types that can be correlated with the sensory texture of snacks. Eleven commercial extruded snacks of different shapes were evaluated instrumentally using different probes and sensorially through descriptive analysis. The snack texture was described using the attributes of hardness, crispness, adhesiveness, fracturability and chewiness. Cylindrical snacks were described through crispness and fracturability, pelleted and shell-shaped snacks by chewiness and ring-shaped snacks by adhesiveness and hardness. Hardness and adhesiveness were correlated with a Warner-Bratzler test using a V shape probe (r = 0.718 and r = 0.763, respectively), while fracturability and chewiness were correlated with a Warner-Bratzler test using a guillotine (r = 0.776 and r = 0.662, respectively). The fairly strong good correlations enable application of these instrumental tests as an indication of the sensory texture of extruded snacks. © 2013 Elsevier Ltd. All rights reserved.

Detecção de Crateras de Impacto em Imagens Digitais. Estudo de caso: Superfície de Marte

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Argilominerais: influência dos aditivos (cinza de bagaço de cana-de-acúcar e rocha sedimentar) no processo de sinterização

Pós-graduação em Ciência e Tecnologia de Materiais - FC

Detecção de microrganismos em bráquetes metálicos in vivo, com ou sem utilização de agente antimicrobiano, pela técnica Checkerboard DNA-DNA Hybridization

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo experimental do escoamento em torno de cilindros circulares em movimento de rotação

Pós-graduação em Engenharia Mecânica - FEIS

Detecção da microbiota endodôntica de dentes sem vitalidade pulpar com ou sem lesão periapical radiográfica, por meio das técnicas de checkerboard DNA-DNA hybridization e cultura microbiológica

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Perspectivas femininas em Helena Morley e Lygia Fagundes Telles: Minha vida de menina e As meninas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Construção de uma junção Josephson em filmes finos supercondutores do sistema Bi1.8Pb0.4Sr2CaCu2Ox tratados termicamente em um forno microondas doméstico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Isolamento e caracterização de elementos transponíveis em espécies do gênero Brycon (Characidae, Bryconinae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Contribuições ao conhecimento de cromossomos supernuméricos em peixes, utilizando como modelo Haplochromis obliquidens (Perciformes, Cichlidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estrutura cromossômica e caracterização cariotípica no complexo de espécies Synbranchus marmoratus (Teleostei, Synbranchiformes, Synbranchidae)

Pós-graduação em Ciências Biológicas (Genética) - IBB

Estudo citogenético da região 17p11.2: a síndrome de Smith-Magenis

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)