Topological locking restrains replication fork reversal.

Two-dimensional agarose gel electrophoresis, psoralen cross-linking, and electron microscopy were used to study the effects of positive supercoiling on fork reversal in isolated replication intermediates of bacterial DNA plasmids. The results obtained demonstrate that the formation of Holliday-like junctions at both forks of a replication bubble creates a topological constraint that prevents further regression of the forks. We propose that this topological locking of replication intermediates provides a biological safety mechanism that protects DNA molecules against extensive fork reversals.

Crucero de evaluación del stock de Merluza en otoño de 1997 BIC Humboldt 9705-06, Callao a Puerto Pizarro. Informe de la componente oceanográfica

Durante el crucero BIC Humboldt 9705-06 la temperatura superficial del mar mostró un incremento de 1°C a 2°C con respecto a lo registrado el mes de abril, originando anomalías positivas de 3,2°C a 5,9°C con excepción del CM 343-09 donde se registró 1,3°C.