954 resultados para MAIZE


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This exploratory study evaluated biophysical, cultural and socio-economic factors affecting crop production and land utilisation in the Nkonkobe Municipality, South Africa. The study sought to establish what farmers in the area perceive as serious threats to crop production, drivers for land abandonment, and how best current agricultural production could be intensified. The farmers’ perspectives were assessed through interviews using semi-structured and open-ended questionnaires. The results of the study revealed declining crop productivity and increase in land abandonment in the Municipality. The biophysical drivers of land abandonment were low and erratic rainfall and land degradation while the socio-economic drivers were labour shortages due to old age and youth movement to cities, lack of farming equipment and security concerns. The most abandoned crops were maize, sorghum and wheat. This trend was attributed to the labour intensiveness of cereal production and a shift in dietary preference to purchased rice. These findings should be factored in any programmes that seek to increase land utilisation and crop productivity in the Municipality.

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In the big cities of Pakistan, peri-urban dairy production plays an important role for household income generation and the supply of milk and meat to the urban population. On the other hand, milk production in general, and peri-urban dairy production in particular, faces numerous problems that have been well known for decades. Peri-urban dairy producers have been especially neglected by politicians as well as non-government-organizations (NGOs). Against this background, a study in Pakistan’s third largest city, Faisalabad (Punjab Province), was carried out with the aims of gathering basic information, determining major constraints and identifying options for improvements of the peri-urban milk production systems. For data collection, 145 peri-urban households (HH) engaged in dairy production were interviewed face to face using a structured and pretested questionnaire with an interpreter. For analyses, HH were classified into three wealth groups according to their own perception. Thus, 38 HH were poor, 95 HH well off and 12 HH rich (26.2%, 65.5% and 8.3%, respectively). The richer the respondents perceived their HH, the more frequently they were actually in possession of high value HH assets like phones, bank accounts, motorbikes, tractors and cars. Although there was no difference between the wealth groups with respect to the number of HH members (about 10, range: 1 to 23), the educational level of the HH heads differed significantly: on average, heads of poor HH had followed education for 3 years, compared to 6 years for well off HH and 8 years for rich HH. About 40% of the poor and well off HH also had off-farm incomes, while the percentage was much higher - two thirds (67%) - for the rich HH. The majority of the HH were landless (62%); the rest (55 HH) possessed agricultural land from 0.1 to 10.1 ha (average 2.8 ha), where they were growing green fodder: maize, sorghum and pearl millet in summer; berseem, sugar cane and wheat were grown in winter. Dairy animals accounted for about 60% of the herds; the number of dairy animals per HH ranged from 2 to 50 buffaloes (Nili-Ravi breed) and from 0 to 20 cows (mostly crossbred, also Sahiwal). About 37% (n=54) of the HH did not keep cattle. About three quarters of the dairy animals were lactating. The majority of the people taking care of the animals were family workers; 17.3% were hired labourers (exclusively male), employed by 11 rich and 32 well off HH; none of the poor HH employed workers, but the percentages were 33.7% for the well off and 91.7% for the rich HH. The total number of workers increased significantly with increasing wealth (poor: 2.0; well off:2.5; rich: 3.4). Overall, 69 female labourers were recorded, making up 16.8% of employed workers and one fourth of the HH’s own labourers. Apparently, their only duty was to clean the animals´ living areas; only one of them was also watering and showering the animals. Poor HH relied more on female workers than the other two groups: 27.1% of the workers of poor HH were women, but only 14.8% and 6.8% of the labour force of well off and rich HH were female. Two thirds (70%) of the HH sold milk to dhodis (middlemen) and one third (35%) to neighbours; three HH (2%) did doorstep delivery and one HH (1%) had its own shop. The 91 HH keeping both species usually sold mixed milk (97%). Clients for mixed and pure buffalo milk were dhodis (78%, respectively 59%) and neighbours (28%, respectively 47%). The highest milk prices per liter (Pakistani Rupees, 100 PKR @ 0.8 Euro) were paid by alternative clients (44 PKR; 4 HH), followed by neighbours (40 PKR, 50 HH); dhodis paid lower prices (36 PKR, 99 HH). Prices for pure buffalo and mixed milk did not differ significantly. However, HH obtaining the maximum price from the respective clients for the respective type of milk got between 20% (mixed milk, alternative clients) and 68% (mixed milk, dhodi) more than HH fetching the minimum price. Some HH (19%) reported 7% higher prices for the current summer than the preceding winter. Amount of milk sold and distance from the HH to the city center did not influence milk prices. Respondents usually named problems that directly affected their income and that were directly and constantly visible to them, such as high costs, little space and fodder shortages. Other constraints that are only influencing their income indirectly, e.g. the relatively low genetic potential of their animals due to neglected breeding as well as the short- and long-term health problems correlated with imbalanced feeding and insufficient health care, were rarely named. The same accounts for problems accompanying improper dung management (storage, disposal, burning instead of recycling) for the environment and human health. Most of the named problems are linked to each other and should be addressed within the context of the entire system. Therefore, further research should focus on systematic investigations and improvement options, taking a holistic and interdisciplinary approach instead of only working in single fields. Concerted efforts of dairy farmers, researchers, NGOs and political decision makers are necessary to create an economic, ecological and social framework that allows dairy production to serve the entire society. For this, different improvement options should be tested in terms of their impact on environment and income of the farmers, as well as feasibility and sustainability in the peri-urban zones of Faisalabad.

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A study was taken to investigate the types of brassica vegetables mostly grown by smallholder farmers in two provinces of South Africa. Thirty-one smallholder vegetable farmers in the Gauteng province and Waterberg district in the Limpopo province were surveyed. In addition, the study also sought to establish the common diseases, the management strategies used and problems encountered by the farmers. Farmers were interviewed using a questionnaire with closed and open–ended questions. The results indicated that the smallholder farmers mostly grew cabbage (93.6%) as their main brassica crop followed by rape (41.2%). Thirty percent of farmers could not identify or name the predominant disease/s encountered in their fields. Major diseases encountered by farmers surveyed were an unknown disease/s (33.3%), black rot (26.7%), Alternaria leaf spot (6.7%) and white rust (6.7%). Smallholder farmers have inadequate technical information available especially relating to crop diseases, their identification and control. Farmers encountered challenges with black rot disease especially on cabbage, rape and kale and the disease was a problem during winter and summer. Generally, the smallholder farmers used crop rotation (74.2%) as a major practice to manage the diseases experienced. They rotated their brassica vegetables with other crops/vegetables like tomatoes, onions, beetroots and maize. Most of the farmers interviewed (61.3%) did not use chemicals to control diseases, whereas 38.7% of them used chemicals. This was mostly because they lacked information and knowledge, high costs associated with use of chemical fungicides and some were shifting towards organic farming. From the study it was noted that there was a need for technical support to improve farmers’ knowledge on disease identification and control within the surveyed areas.

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In the ornamental plant production region of Girona (Spain), which is one of the largest of its kind in southern Europe, most of the surface is irrigated using wide blocked-end furrows. The objectives of this paper were: (1) to evaluate the irrigation scheduling methods used by ornamental plant producers; (2) to analyse different scenarios in order to assess how they affect irrigation performance; (3) to evaluate the risk of deep percolation; and (4) to calculate gross water productivity. A two-year study in a representative commercial field, planted with Prunus cerasifera ‘Nigra’, was carried out. The irrigation dose applied by the farmers was slightly smaller than the required water dose estimated by the use of two different methods: the first based on soil water content, and the second based on evapotranspiration. Distribution uniformity and application efficiency were high, with mean values above 87%. Soil water content measurements revealed that even at the end of the furrow, where the infiltrated water depth was greatest, more than 90% of the infiltrated water was retained in the shallowest 40 cm of the soil; accordingly, the risk of water loss due to deep percolation was minimal. Gross water productivity for ornamental tree production was € 11.70 m–3, approximately 20 times higher than that obtained with maize in the same region

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Aquesta tesi doctoral s'engloba dins d'un projecte general d'estudi de gens implicats en l'embriogènesi del blat de moro. L'embriogènesi del blat de moro, i en general la de totes les plantes superiors, es dóna en tres etapes: una primera etapa on es diferencien tots els diversos teixits que formaran l'embrió, una segona etapa on l'embrió acumula productes de reserva i un tercer període, la dormància, que finalitza quan les condicions ambientals són les idònies per a la germinació. En el laboratori estàvem interessats, concretament, en l'estudi de gens implicats en la primera etapa morfogenètica, on els diferents teixits i estructures embrionàries queden definides. Per tal d'estudiar gens que s'expressaven en aquest període, una de les estratègies que es va realitzar fou un crivellat diferencial entre teixit embrionari i teixit de planta adulta. D'entre els diferents clons obtinguts, un corresponia a un clon parcial que presentava similitud amb receptors quinasa i que fou objecte d'estudi. A partir d'aquest clon es va obtenir el clon complet i es va anomenar MARK (per Maize Atypical Receptor Kinase). MARK presenta una estructura típica d'un receptor quinasa amb un domini extracel.lular, que conté 6 còpies imperfectes de LRR (Leucine- Rich Repeats), un únic domini transmembrana i un domini quinasa intracel.lular. El domini quinasa de MARK presenta, però, algunes variacions en els residus aminoacídics que es consideren claus per a la funció catalítica dels dominis quinasa. En concret cinc dels aminoàcids considerats essencials per a la fosforilació es troben substituits en el domini quinasa de MARK (DK-MARK). Els experiments de fosforilació in vitro que es van realitzar al laboratori, van mostrar com MARK era incapaç de fosforilar in vitro. Aquesta característica no és, però, exclusiva de MARK. Una búsqueda en les bases de dades ens van permetre identificar altres seqüències que també presentaven els mateixos o altres canvis en aquestes posicions aminoacídiques. En les bases de dades de plantes es van identificar un conjunt de seqüències genòmiques o ESTs amb aquestes característiques i només una d'elles, la proteïna TMKL1 d'Arabidopsis, ha sigut descrita com un receptor quinasa incapaç de fosforilar in vitro. Respecte a la búsqueda de receptors similars a MARK en les bases de dades d'animals, es van identificar també un conjunt de proteïnes que, en alguns casos, s'ha descrit que no tenen activitat quinasa in vivo. Per exemple, un dels casos més ben estudiats és el del receptor erbB3 que forma part de la família de receptors del EGF (Epidermal Growth Factor). Aquesta família de receptors està formada per 4 receptors: erbB1, erbB2, erbB3 i erbB4, dels quals només l'erbB3 no presenta activitat catalítica. S'ha descrit que erbB3 és capaç, tot i no fosforilar in vivo, de participar activament en la transducció del senyal formant heterodímers amb els altres membres de la família. Així, erbB3 és fosforilat pel seu partner i pot iniciar la cascada de transducció del senyal. La participació d'erbB3 en la transducció del senyal és essencial ja que embrions de ratolí knock-out pel gen erbB3 són inviables. Així doncs, el fet que receptors quinasa catalíticament inactius participin en les cascades de transducció del senyal, suggereix l'existència de nous mecanismes d'acció per a la transducció del senyal. Per tant, l'objectiu d'aquest treball fou l'estudi del mecanisme d'acció de MARK mitjançant la caracterització les proteïnes capaces d'interaccionar amb el seu domini quinasa. Per tal d'assolir aquest objectiu, es va realitzar un crivellat de doble-híbrid amb una llibreria de cDNA d'embrions de blat de moro de 7 DAP. D'aquest crivellat es va obtenir un conjunt de possibles clons positius que foren seqüenciats i entre els quals es van escollir per un estudi més detallat aquells que s'havien obtingut més vegades com a clons independents. Aquests clons codificaven per: una SAMDC (S-Adenosil Descarboxilasa), una eIF5 (Eukaryotic translation initiation), una hypothetical protein, una unknown protein, una gamma-adaptina i una MAP4K. Amb aquests 6 clons es van fer estudis in vitro i in vivo per tal de confirmar al seva interacció amb DK-MARK. Els estudis in vivo es van realitzar amb la soca de llevat AH109, una soca més astringent que la utilitzada en el crivellat, ja que presenta tres gens marcadors: Histidina, Adenina i Lacz. Els resultats obtinguts van mostrar que els clons codificants per SAMDC i eIF5 no van créixer en un medi selectiu per His i Ade i, per tant o es tracta de falsos positius del sistema o la seva interacció amb DK-MARK és dèbil. D'altra banda, la resta dels clons analitzats (proteïna hipotètica, una proteïna de funció desconeguda, la gamma-adaptina i una MAP4K) van créixer en medis en absència de Histidina i Adenina. Els assatjos de b-galactosidasa van ser tots positius a excepció de la proteïna hipotètica suggerint que potser aquesta interacció sigui més feble. D'altra banda també es van realitzar estudis in vitro amb la tècnica del pull-down. Els resultats obtinguts amb aquesta tècnica van recolzar els obtinguts en cèl.lules de llevat, ja que tots els clons analitzats a excepció dels codificants per SAMDC i eIF5 van donar un resultat d'interacció amb KD-MARK in vitro positiu. Davant aquests resultats ens vam centrar en l'estudi de la proteïna similar a MAP4K, doncs algunes proteïnes de la seva família s'han relacionat amb receptors de membrana. Els clons que es va obtenir del crivellat codificaven per una proteïna similar amb el domini C-terminal a les proteïnes BnMAP4Ka1 i a2 de Brassica napus. Aquestes proteïnes presenten una forta similitud de seqüència amb proteïnes de la família GCK/SPS1 que formen part d'un grup particular de MAPK relacionades amb la proteïna Ste20 (sterile 20 protein) de llevat. Ste20p activa la MAP3K de llevat Ste11 directament per fosforilació, transduint d'aquesta manera el senyal del receptor de feromones de creuament de les cèl.lules de llevat i es pot, doncs, considerar com una proteïna del tipus MAP4K (mitogen-activated protein kinase kinase kinase kinase). En els darrers anys, s'han identificat un gran nombre de proteïnes similars a Ste20: fins a una trentena en mamífers, en Drosophila, en Caenorhabditis elegans i en altres organismes. Segons la seva estructura aminoacídica, la família Ste20 s'ha classificat en dues subfamílies: les proteïnes STE20/PAK (p21-activated kinases) i la subfamília GCK/SPS1 (germinal center kinases). Les dues subfamílies estan formades per proteïnes que contenen un domini quinasa i un domini regulador, però, mentre que les proteïnes PAK presenten el domini quinasa en la part C-terminal, les GCKs el presenten en la regió N terminal. Les proteïnes GCK presenten una elevada diversitat estructural en el domini regulador permetent la seva classificació en 6 subfamílies. Mitjançant la tècnica del RACE es va obtenir el clon de cDNA complet que es va anomenar MIK (MARK Interacting Kinase). Amb la tècnica del Southern blot es va poder determinar que el gen MIK és un gen de còpia única en el genoma de blat de moro. Per tal d'analitzar la possible interacció entre DK-MARK i MIK, es va estudiar tant el patró d'expressió d'ambdós gens com el seu patró d'acumulació d'ambdues proteïnes durant l'embriogènesi del blat de moro. El patró d'expressió, analitzat per Northen blot va mostrar uns patrons coincidents al llarg de l'embriogènesi des del seu inici fins als 20 DAP amb una acumulació màxima de mRNA en embrions de 15 DAP. D'altra banda per tal d'estudiar el patró d'acumulació de la proteïna MIK així com per comparar-lo amb el de MARK, es van realitzar estudis de Westerns blot. Els resultats també van mostrar una coincidència en el temps de l'acumulació de les proteïnes MARK i MIK durant l'embriogènesi de blat de moro amb una major acumulació en embrions de 15 i 20 DAP. Es van dur a terme també estudis d'immunolocalitzacions sobre embrions de blat de moro de 15 DAP per tal d'estudiar en quins teixits s'acumulaven ambdues proteïnes. Les immunolocalitzacions van mostrar una major acumulació tant de MARK com de MIK en les zones meristemàtiques i en el teixit vascular sobretot del coleòptil on s'aprecia una forta co-localització de MARK i MIK. Totes aquestes dades són compatibles, doncs, amb una possible interacció de les proteïnes MARK i MIK, tot i que no la demostren. Per tal de demostrar la interacció es van realitzar experiments d'immunoprecipitació in vivo a partir d'extractes d'embrions. Malauradament, els resultats no són clars i en aquests moments en el laboratori s'estan posant a punt aquests experiments. També es van realitzar estudis comparatius de seqüència amb diferents proteïnes de la família GCK, mostrant una major similitud amb les proteïnes de la subfamília GCK-III. La subfamília GCK-III ha estat molt poc estudiada i en formen part un conjunt de proteïnes amb funcions molt diverses des de l'apoptosi, la citoquinesi o l'anòxia cel.lular. Per tant, la similitud de seqüència possiblement fa referència a una conservació en el mecanisme d'acció més que no pas a una conservació funcional. La possible interacció de MARK amb el domini C-terminal de MIK (el domini regulador) podria activar aquesta última iniciant una cascada de transducció del senyal en un model en el que una proteïna del tipus GCK-III faria de lligam directa entre un receptor de membrana i una cascada de senyalització intracel.lular. Aquest tipus de lligam entre un recepctor de membrana i mòduls intracel.lulars de senyalització s'ha descrit per a altres proteïnes GCK, si bé no directament sinó a través de proteïnes adaptadores. D'altra banda, la interacció directa de MARK, un receptor quinasa atípic que no té activitat catalítica, amb MIK suggereix un mecanisme on receptors atípics podrien interaccionar en la transducció del senyal activant la via de les MAPK.

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Amb la finalitat de garantir la seguretat dels consumidors i del medi ambient, a la UE els aliments modificats genèticament (MG) estan sotmesos a un rigorós procés d'autorització que inclou: caracterització molecular del transgèn i anàlisis comparatives a nivell nutricional i agronòmic. L'ús de tècniques de profiling per avaluar la possible existència d'efectes no esperats derivats de la inserció i/o expressió del transgèn s'ha proposat com a una eina complementària per l'avaluació de la seguretat alimentària. L'objectiu de la present tesis és avaluar la variabilitat associada a la inserció i expressió de transgens en plantes, utilitzant com a exemple el blat de moro MON810. Es pretén complementar les aproximacions ja existents basades en l'estudi de paràmetres concrets mitjançant les tècniques de profiling. Des del punt de vista transcriptòmic i proteòmic, les varietats de blat de moro MON810 semblen ser substancialment equivalents a les seves varietats comparables no-MG. En conseqüència, és possible la producció de plantes MG amb el mínim d'efectes no esperats.

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Sensible and latent heat fluxes are often calculated from bulk transfer equations combined with the energy balance. For spatial estimates of these fluxes, a combination of remotely sensed and standard meteorological data from weather stations is used. The success of this approach depends on the accuracy of the input data and on the accuracy of two variables in particular: aerodynamic and surface conductance. This paper presents a Bayesian approach to improve estimates of sensible and latent heat fluxes by using a priori estimates of aerodynamic and surface conductance alongside remote measurements of surface temperature. The method is validated for time series of half-hourly measurements in a fully grown maize field, a vineyard and a forest. It is shown that the Bayesian approach yields more accurate estimates of sensible and latent heat flux than traditional methods.

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The input to soils made by pollen and its subsequent mineralization has rarely been investigated from a soil microbiological point of view even though the small but significant quantities of C and N in pollen may make an important contribution to nutrient cycling. The relative resistance to decomposition of pollen exines (outer layers) has led to much of the focus of pollen in soil being on its preservation for archaeological and palaeo-ecological purposes. We have examined aspects of the chemical composition and decomposition of pollen from birch (Betula alba) and maize (Zea mays) in soil. The relatively large N contents, small C-to-N ratios and large water-soluble contents of pollen from both species indicated that they would be readily mineralized in soil. When added to soil and incubated at 16 degrees C an amount of C equivalent to 22-26% of the added pollen C was lost as CO2 within 22 days, with the Z. mays pollen decomposing faster. For B. alba pollen, the water-soluble fraction decomposed faster than the whole pollen and the insoluble fraction decomposed more slowly over 22 days. By contrast, there were no significant differences in the decomposition rates of the different fractions from Z. mays pollen. Solid-state C-13 nuclear magnetic resonance (NMR) revealed no gross chemical differences between the pollen of these two species, with strong resonances in the alkyl- and methyl-C region (0-45 p.p.m.) indicative of aliphatic compounds, the O-alkyl-C (60-90 p.p.m.) and the acetal- and ketal-C region (90-110 p.p.m.) indicative of polysaccharides, and the carbonyl-C region indicative of peptides and carboxylic acids. In addition, both pollens gave a small but distinct resonance at 55 p.p.m. attributed to N-alkyl-C. The resonances attributed to polysaccharides were lost completely or substantially reduced after decomposition.

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Zinc deficiency is the most ubiquitous micronutrient deficiency problem in world crops. Zinc is essential for both plants and animals because it is a structural constituent and regulatory co-factor in enzymes and proteins involved in many biochemical pathways. Millions of hectares of cropland are affected by Zn deficiency and approximately one-third of the human population suffers from an inadequate intake of Zn. The main soil factors affecting the availability of Zn to plants are low total Zn contents, high pH, high calcite and organic matter contents and high concentrations of Na, Ca, Mg, bicarbonate and phosphate in the soil solution or in labile forms. Maize is the most susceptible cereal crop, but wheat grown on calcareous soils and lowland rice on flooded soils are also highly prone to Zn deficiency. Zinc fertilizers are used in the prevention of Zn deficiency and in the biofortification of cereal grains.

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An X-ray micro-tomography system has been designed that is dedicated to the low-dose imaging of radiation sensitive living organisms and has been used to image the early development of the first few days of plant development immediately after germination. The system is based on third-generation X-ray micro-tomography system and consists of an X-ray tube, two-dimensional X-ray detector and a mechanical sample manipulation stage. The X-ray source is a 50 kVp X-ray tube with a silver target with a filter to centre the X-ray spectrum on 22 keV.A 100 mm diameter X-ray image intensifier (XRII) is used to collect the two-dimensional projection images. The rotation tomography table incorporates a linear translation mechanism to eliminate ring artefact that is commonly associated with third-generation tomography systems' Developing maize seeds (Triticum aestivum) have been imaged using the system with a cubic voxel linear dimension of 100 mum, over a diameter of 25 mm and the root lengths and volumes measured. The X-ray dose to the plants was also assessed and found to have no effect on the plant root development. (C) 2003 Elsevier Science Ltd. All rights reserved.

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Long-term indicators of soil fertility were assessed by measuring grain yield, soil organic carbon (SOC) and soil Olsen phosphorous for a P-deficient soil. In one set of treatments, goat manure was applied annually for 13 years at 0, 5 and 10 t ha(-1), and intercrops of sorghum/cowpea, millet/green gram and maize/pigeonpea were grown. Yield depended on rainfall and trends with time were not identifiable. Manure caused an upward trend in SOC, but 10 t ha(-1) manure did not give significantly more SOC than 5 t ha(-1). Only 10 t ha(-1) manure increased Olsen P. Measurements of both SOC and Olsen P are recommended. In another set of treatments, manure was applied for four years; the residual effect lasted another seven to eight years when assessed by yield, SOC and Olsen P Treatment with mineral fertilizers provided the same rates of N and P as 5 t hat manure and yields from manure and fertilizer were similar. Fertilizer increased Olsen P but not SOC. Management systems with occasional manure application and intermediate fertilizer applications should be assessed. Inputs and offtakes of C, N and P were measured for three years. Approximately 16, 25 and 11% of C, N and P respectively were stabilized into soil organic matter from 5 t ha(-1) a(-1) manure. The majority of organic P was fixed as soil inorganic P.

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The purpose of this study was to test the hypothesis that soil water content would vary spatially with distance from a tree row and that the effect would differ according to tree species. A field study was conducted on a kaolinitic Oxisol in the sub-humid highlands of western Kenya to compare soil water distribution and dynamics in a maize monoculture with that under maize (Zea mays L.) intercropped with a 3-year-old tree row of Grevillea robusta A. Cunn. Ex R. Br. (grevillea) and hedgerow of Senna spectabilis DC. (senna). Soil water content was measured at weekly intervals during one cropping season using a neutron probe. Measurements were made from 20 cm to a depth of 225 cm at distances of 75, 150, 300 and 525 cm from the tree rows. The amount of water stored was greater under the sole maize crop than the agroforestry systems, especially the grevillea-maize system. Stored soil water in the grevillea-maize system increased with increasing distance from the tree row but in the senna-maize system, it decreased between 75 and 300 cm from the hedgerow. Soil water content increased least and more slowly early in the season in the grevillea-maize system, and drying was also evident as the frequency of rain declined. Soil water content at the end of the cropping season was similar to that at the start of the season in the grevillea-maize system, but about 50 and 80 mm greater in the senna-maize and sole maize systems, respectively. The seasonal water balance showed there was 140 mm, of drainage from the sole maize system. A similar amount was lost from the agroforestry systems (about 160 mm in the grevillea-maize system and 145 mm in the senna-maize system) through drainage or tree uptake. The possible benefits of reduced soil evaporation and crop transpiration close to a tree row were not evident in the grevillea-maize system, but appeared to greatly compensate for water uptake losses in the senna-maize system. Grevillea, managed as a tree row, reduced stored soil water to a greater extent than senna, managed as a hedgerow.

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This paper presents the results of (a) On-farm trials (eight) over a two-year period designed to test the effectiveness of leguminous cover crops in terms of increasing maize yields in Igalaland, Nigeria. (b) A survey designed to monitor the extent of, and reasons behind, adoption of the leguminous cover crop technology in subsequent years by farmers involved, to varying degrees, in the trial programme. particular emphasis was placed on comparing adoption of leguminous cover crops with that of new crop varieties released by a non-governmental organization in the same area since the mid 1980s. While the leguminous cover crop technology boosted maize grain yields by 127 to 136% above an untreated control yield of between 141 and 171 kg ha(-1), the adoption rate (number of farmers adopting) was only 18%. By way of contrast, new crop varieties had a highly variable benefit in terms of yield advantage over local varieties, with the best average increase of around 20%. Adoption rates for new crop varieties, assessed as both the number of farmers growing the varieties and the number of plots planted to the varieties, were 40% on average. The paper discusses some key factors influencing adoption of the leguminous cover crop technology, including seed availability. Implications of these results for a local non-governmental organization, the Diocesan Development Services, concerned with promoting the leguminous cover crop technology are also discussed.

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The effects of maize and soya bean residues on the pH and charge of a loamy sand (Kawalazi) and a sandy clay loam (Naming'omba) from Malawi were measured to determine both the indirect effect of the residues on soil charge through the changes in pH, and the direct contribution of charge carried on the residue surfaces. The soils had pH values (10 mM CaCl2) of 4.3 and 5.0 and organic matter contents were 1.4% and 2.7%, respectively. The clay fractions were dominated by kaolinite and goethite, and mica was present in both samples. The soils were incubated for 28 days with maize (Zea mays) and soya bean (Glycine max) residues. The maximum addition of residue (12.0%) in the Kawalazi and Naming'omba soils increased the pH from 4.3 and 5.0 to 4.8 and 5.3 (maize) and to 9.0 and 8.8 (soya bean), respectively. Negative charge increased from 2.1 and 4.7 cmol(c) kg(-1) to 3.8 and 7.5 (maize) and to 5.3 and 9.3 cmol(c) kg(-1) (soya bean). Positive charge increased from 0.72 and 0.62 to 0.87 and 0.85 cmol(c) kg(-1) (maize) and to 0.75 and 0.68 (soya bean). The charge contribution by the residues was calculated by difference between the charge on a sample incubated with residue and the charge on a soil without residue limed to the same pH value. Up to 100 cmolc negative charge and 10 cmol(c) of positive charge per kg of residue were directly contributed to the soil-residue mixture, the amounts depending on the type of residue, the extent to which the residue was decomposed in the soil and the pH of the mixture. The Anderson and Sposito method [Soil Sci. Soc. Am. J. 55 (1991) 1569] was used to partition the permanent negative charge (holding Cs+) from variable negative charge (holding Li+). In the pH range 3.7-6.5 the maize residue contributed between 3 and 26 cmol(c) of variable charge per kg of residue in the Kawalazi soil and between 6 and 25 cmol(c) per kg of residue in the Naming'omba soil. For soya bean the values were between I and 28 and between 4 and 68 cmolc per kg of residue, respectively. At a given pH value, the charge tended to increase with time of incubation and for a given addition of residue, pH decreased during incubation. Addition of residues contributed no permanent negative charge and the charge on the soil measured by Cs adsorption was independent of pH change caused by the residue showing that the method is valid for soil-residue mixtures. With time there was a decrease in the amount of permanent charge probably due to masking as humic material become adsorbed on mineral surfaces. (C) 2003 Elsevier Science B.V. All rights reserved.

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Intensification of crop production in the mid-hills of Nepal has led to concerns that nitrogen loss by leaching may increase. This study estimated the amount of N leached during two years from rainfed terraces (bari-land) at three locations in Nepal. Maize or upland rice grown in the monsoon season was given either no nutrient inputs or inputs via either nitrogen fertilizer or farmyard manure. Nitrate concentration in soil solution was measured regularly with porous ceramic cup samplers and drainage estimated from a simple soil water balance. Estimated losses of nitrogen by leaching ranged from 0 to 63.5 kg N ha(-1) depending on location and the form of nitrogen applied. Losses from plots receiving no nutrient inputs were generally small (range: 0-35 kg N ha(-1)) and losses from plots where nitrogen was applied as manure (range: 2-41 kg N ha(-1)) were typically half those from plots with nitrogen applied as fertilizer. Losses during the post-monsoon crops of finger millet were small (typically <5% of total loss) although losses from the one site with blackgram were larger (about 13%). The highest concentrations of nitrate in solution were measured early in the season as the monsoon rains began and immediately following fertilizer applications. Leaching losses are likely to be minimised if manure is applied as a basal nutrient dressing followed by fertilizer nitrogen later in the season.