Efeitos da privação de sono paradoxal na resposta inflamatória de ratos e avaliação do efeito antinociceptivo do ATL-1, um análogo sintético de 15-epi-lipoxinas

Sono e imunidade parecem apresentar uma relação de reciprocidade. A ativação do sistema imune altera o padrão de sono e distúrbios do sono podem afetar a função imune. Além disso, é bem descrito que a privação de sono paradoxal (PSP) leva à hiperalgesia e o tratamento com fármacos clássicos, como opióides ou antidepressivos tricíclicos, não é capaz de reverter este quadro. Neste trabalho, avaliamos se a PSP afetaria a resposta inflamatória e a sobrevida em ratos e se o tratamento com um análogo sintético de lipoxinas (ATL-1) seria capaz de reverter a hiperalgesia induzida pela PSP. Todos os protocolos experimentais foram previamente aprovados pelo Comitê de Ética para o Uso de Animais, da UERJ (CEUA/032/2010). Ratos Wistar machos foram submetidos a 96 h de PSP, induzidas pelo método de plataforma única (PU) ou de múltiplas plataformas modificado (MPM). Após 96 h de PSP os animais foram submetidos ao modelo da bolha de ar ou pleurisia utilizando-se a carragenina como agente flogístico, ou ainda a PSP foi aplicada antes ou após a indução de um modelo de ligação e perfuração do ceco (CLP). Quatro horas após a injeção de carragenina os animais apresentaram um aumento no recrutamento de leucócitos para a cavidade da bolha, porém não houve diferença entre animais PSP e controles. O número total de leucócitos no plasma não se alterou após a injeção de carragenina. Na pleurisia, os animais PSP apresentaram um aumento nos níveis de IL-6, IL-1β e TNF-α no plasma, enquanto apenas IL-1β e IL-6 estavam aumentados no exsudato pleural dos animais que receberam carragenina. O padrão de recrutamento de leucócitos para o local da injúria foi bastante semelhante entre os animais controle e PSP 2 h, 4 h e 24 h após a injeção de carragenina. Houve um aumento progressivo com o tempo, apresentando um pico em 24 h, no entanto, não foi observada diferença significativa na resposta dos grupos PSP. A PSP aplicada antes ou após a indução do CLP reduziu a sobrevida dos animais, mas não alterou o acúmulo de neutrófilos, nos dois protocolos. Quando a PSP foi aplicada antes do CLP, os níveis séricos de IL-6 estavam aumentados nos grupos PSP e PSPCLP, porém quando a PSP foi aplicada após o CLP, ambas IL-6 e IL-1β estavam aumentadas nos grupo PSPCLP. O efeito do tratamento com ATL-1 (10 g/kg, i.v.) na hiperalgesia induzida pela PSP foi determinado através do teste da formalina. O análogo reduziu o número de comportamentos relacionados à dor em animais PSP e controles na fase inflamatória do teste. Nossos resultados demonstraram que a PSP por 96 h aumentou os níveis plasmáticos de citocinas, reduziu a sobrevida dos animais, contudo não foi capaz de alterar o recrutamento de leucócitos frente a um estímulo inflamatório ou infeccioso. O aumento de mediadores inflamatórios observado nesses animais pode estar relacionado à hiperalgesia em animais PSP, uma vez que o tratamento com o ATL-1 reverteu esse efeito, possivelmente através de mecanismos envolvendo sua ação anti-inflamatória.

Participatory action research on climate risk management, Bangladesh

The rural populations of southern Bangladesh are some of the most vulnerable communities in the world to the future impacts of climate change. They are particularly at risk from floods, waterlogged soils, and increasing salinity of both land and water. The objective of this project was to analyze the vulnerability of people in four villages that are experiencing different levels of soil salinity. The study evaluated the strengths and weaknesses of current coping strategies and assessed the potential of an index-based insurance scheme, designed diversification and better information products to improve adaptive capacity.

Regional Fisheries Advisory Committee 29th April, 1974

This is the first report from the Regional Fisheries Advisory Committee meeting, which was held on the 29th April, 1974. The report looks at sections on estimates for the anticipated income and expenditure on fisheries for the current year, the structure of fisheries in the region, and a list of the fisheries in the ownership of the North West Water Authority. Also covered is information relating to fishery and sea fishery byelaws, license duties and a summary of the current activity and the future plans for the committee. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

South & West Cumberland Fisheries Advisory Committee 3rd June, 1974

This is the first report from the South and West Cumberland Fisheries Advisory Committee meeting, which was held on the 3rd June 1974. The report includes sections on fishery byelaws, estimates for the anticipated income and expenditure on fisheries for the current year, and fish mortality. The section on fish mortality includes the details of a fish kill on the River Marron, gives points along the river where water was sampled, and the results of the analysis. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

Eden Fisheries Advisory Committee 4th June, 1974

This is the first report from the Eden Fisheries Advisory Committee meeting, which was held on the 4th June 1974. The report includes sections on fishery byelaws, estimates for the anticipated income and expenditure on fisheries for the current year, licence duties, the Nursery Stream Project and Holmewrangle hatchery. The section on the Nursery Stream Project includes the reasons for undertaking the project, and the details of the project. The section on the Holmewrangle hatchery looks at low survival rates and reasons for mortality for 1973/1974. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

Lune, Wyre & Furness Fisheries Advisory Committee 6th June, 1974

This is the first report from the Lune, Wyre and Furness Fisheries Advisory Committee meeting, which was held on the 6th June 1974. The report includes sections on fishery byelaws, estimates for the anticipated income and expenditure on fisheries for the current year, the arrangements for the release of freshets to the River Leven in times of drought, and the utilisation of water resources. This section looks at proposed abstractions for the River Lune and Wyre, and the Ullswater and Windermere Pumping Stations. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

South Lancashire Advisory Committee 10th June, 1974

This is the first report from the South Lancashire Fisheries Advisory Committee meeting, which was held on the 10th June 1974. The report includes sections on fishery byelaws, estimates for the anticipated income and expenditure on fisheries for the current year, and information includeing the aims of a report entitled 'Taking Stock', which looked at migratory fish in England and Wales. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

Regional Fisheries Advisory Committee 21st July, 1975

This is the report from the Regional Fisheries Advisory Committee meeting, which was held on the 21st July 1975. The report contains information on the appointment of the Regional Fisheries Advisory Committee (1975-1976), the appointment of the Local Fisheries Advisory Committee (1975-1976), and the land drainage representation on Local Fisheries Committees. Also included in the report is information on fishery byelaws, fishing at Jumble Reservoir and a progress report on a proposed Coarse Fish Unit and coarse fish stock pools. Licence duties, long term angling leases on Authority Waters and radio equipment for Bailiffting Staff are also covered. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

Mersey & Weaver Advisory Committee 12th June, 1974

This is the first report from the Mersey and Weaver Fisheries Advisory Committee meeting, which was held on the 12th June 1974. The report includes sections on fishery byelaws, estimates for the anticipated income and expenditure on fisheries for the current year, information including the aims of a report entitled 'Taking Stock', licence duties, the need of a Coarse Fish Unit and pollution. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

South & West Cumberland Fisheries Advisory Committee 2nd September, 1974

This is the report from the South and West Cumberland Fisheries Advisory Committee meeting, which was held on the 2nd September 1974. The report looks at comments of the Regional Fisheries Officer on the report 'Taking Stock', and information including the aims of a report entitled 'Coarse Fisheries'. The report also includes sections on the summary of the stocking carried out from Holmewrangle hatchery, biological work undertaken on the Rivers Ellen, Keekle and Thirlmere Reservoir, fishery prosecutions, and fish mortalities. This section includes information on the date, location, extent of the kill and the cause. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

South & West Cumberland Fisheries Advisory Committee 13th October, 1975.

This is the report from the South and West Cumberland Fisheries Advisory Committee meeting, which was held on the 13th October 1975. The report contains information on the impact of work on the A66 Penrith to Workington road, brown trout fishing, the development of the Ehen as a fishery, reports of fisheries activities, Holmwrangle hatchery, land drainage representation, new fishery byelaws and fishing licence duties. The section on fisheries activities includes runs of fish, stocking, poaching and biological work. The section on Holmwrangle hatchery includes mortality numbers and possible solutions to avoid future pipe chokes and to improve the pumping system. The section on land drainage is purely for an up-to-date picture of developments. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

Eden Fisheries Advisory Committee 3rd September, 1974

This is the report from the Eden Fisheries Advisory Committee meeting, which was held on the 3rd September 1974. The report looks at comments of the Regional Fisheries Officer on the report 'Taking Stock', and information including the aims of a report entitled 'Coarse Fisheries'. The report also includes sections on the summary of the stocking carried out from Holmewrangle hatchery, biological work undertaken on the Rivers Liddel, fishery prosecutions, and fish mortalities. This section includes information on the date, location, extent of the kill and the cause. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

Lune, Wyre & Furness Fisheries Advisory Committee 9th September, 1974

This is the report from the Lune, Wyre and Furness Fisheries Advisory Committee meeting, which was held on the 9th September 1974. The report looks at comments of the Regional Fisheries Officer on the report 'Taking Stock', and information including the aims of a report entitled 'Coarse Fisheries'. The report also includes sections on the improvement of fisheries in the River Leven, coarse fish salvage and stocking, fishery prosecutions, and biological work on the River Kent. Fish mortalities are also given as well as fish monitoring figures for areas on the Rivers Lune and Leven for 1974. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.