EVALUATION OF DIFFERENT TECHNIQUES FOR THE DIFFERENTIATION OF PATHOGENIC AND NON PATHOGENIC STRAINS OF YERSINIA-ENTEROCOLITICA

Different methods and tests have been used to evaluate the pathogenic potential of distinct Y. enterocolitica serotypes and biotypes. We tested a total of 60 Y. enterocolitica strains, being 25 of human origin (serotype O3 biotype 4 and serotype O5 biotype 1); 6 of animal origin (serotype O3 biotype 4); 19 isolated from the environment (serotype O5.27 biotypes 1 and 2); and 8 isolated from food (serotype O5 biotype 1 and serotype 05.27 biotype 1). The methods used were based on plasmid gene expression (autoagglutination, calcium-dependence at 37 degrees C and Congo Red absorption tests), chromosomal gene expression (assays for pyrazinamidase activity, salicin fermentation and esculin hydrolysis), and invasion of HEp-2 cells. All but one of the Y. enterocolitica O3 strains, were found to be potentially pathogenic when submitted to the pyrazinamidase-salicin-esculin tests. In contrast, the results obtained with the assays related to plasmidial gene expression were not so uniform, probably due to plasmid loss. The least homogeneous results were obtained with the HEp-2 cell invasion test. Y. enterocolitica O5 behaved in a uniform manner when tested with the first two groups of tests (based on chromosomal and plasmidial gene expression), but not when tested with the HEp-2 invasion assay. The strains of serotype O5.27 biotype 1 presented a uniform behavior hen submitted to the chromosomic-related tests, showing no pathogenicity. However, they did not provide conclusive results with the tests related to plasmidial gene expression or HEp-2 cell invasion. We conclude that the tests related to chromosomal gene expression (esculin-salicin-pyrazinamidase) are simple and highly effective for the detection of potentially pathogenic Y. enterocolitica isolated from clinical cases.

Rotor support stiffness estimation by sensitivity analysis

In the present work, a method for rotor support stiffness estimation via a model updating process using the sensitivity analysis is presented. This method consists in using the eigenvalues sensitivity analysis, relating to the rotor support stiffnesses variation to perform the adjustment of the model based on the minimization of the difference between eigenvalues of reference and eigenvalues obtained via mathematical model from previously adopted support bearing stiffness values. The mathematical model is developed by the finite element method and the method of adjustment should converge employing an iterative process. The performance and robustness of the method have been analyzed through a numerical example.

Discrete approximations for strict convex continuous time problems and duality

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Effect of sowing date on the behavior of 2 genotypes of sunflower .1. phenological and agronomical aspects

Este experimento foi realizado em .iaboticabal, SP, com o objetivo de se avaliar o comportamento de dois genótipos de girassol (líeIianthus annuus L.) no tocante à produção dc matéria seca e caracte r ísticas agronômicas. Utilizou-se o híbrido Contissol 812 e a cultivar IAC-Anhandy com 80 cm entre linhas e 5 plantas por metro linear, em blocos casualizados, com quatro repetições cm cinco datas de semcadura: 2001, 2002, 05.03, 2003 e 05.04. O atraso das datas de semeadura exerceu efeito depressivo e significativo sobre a produção e distribuição de matéria seca (kg/ha) no caule, folha, capítulo e no total, reduzindo também significativamente o número de dias de emergência ao florescimento, a altura das plantas, o número de folhas, e os diãmetros do colo e do capitulo. A produ- ção média de aqüõnios foi de: 2.942, 2.005, 1.243. 1.073 e 809 kg/ha, da primeira ã última data, rcspectivamente, nos dois genótipos. Concluiu-se que os dois genótipos se comportaram de forma scmc' lhante nas diferentes datas, e que as semeaduras realizadas até o final de fevereiro foram as melhores, e nas efetuadas posteriormente, o crescimento e o desenvolvimento das plantas foram afetados negativamente, levando a produção de aqüênios a níveis indesejáveis.