Consórcios de pepino e alface em cultivo protegido: viabilidade agroeconômica

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Armazenamento refrigerado e processamento mínimo de alface americana cultivada em hidroponia com diferentes doses de silício

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Processo de obtenção de componentes ativos de própolis no estado sólido

Processo de obtenção de componentes ativos de própolis no estado sólido realizado sem a utilização de etanol em seus extratos, possibilitando a obtenção destes elementos em seu estado sólido, facilitando assim seu manuseio, armazenamento, transporte e acondicionamento, sendo que para a obtenção de tais resultados, o presente processo utiliza-se de microencapsulação, que tem sido amplamente empregada com o objetivo de mascarar o sabor e o odor desagradáveis, de controlar a liberação, facilitando o cumprimento da posologia por parte do paciente e auxiliando na terapêutica, sendo que esta tecnologia também possui a propriedade de transformar formas farmacêuticas líquidas em sólidas, eliminando solventes indesejáveis e promovendo fácil manuseio.

Influência da fuligem da queima de palha de cana-de-açucar na presença de HPA em hortaliças de consumo humano

Pós-graduação em Química - IQ

Mesenchymal stem cells modulate release of matrix proteins from tendon surfaces in vitro: a potential beneficial therapeutic effect

Aim: Injury of tendons contained within a synovial environment, such as joint, bursa or tendon sheath, frequently fails to heal and releases matrix proteins into the synovial fluid, driving inflammation. This study investigated the effectiveness of cells to seal tendon surfaces and provoke matrix synthesis as a possible effective injectable therapy. Materials & methods: Equine flexor tendon explants were cultured overnight in suspensions of bone marrow and synovium-derived mesenchymal stems cells and, as controls, two sources of fibroblasts, derived from tendon and skin, which adhered to the explants. Release of the most abundant tendon extracellular matrix proteins into the media was assayed, along with specific matrix proteins synthesis by real-time PCR. Results: Release of extracellular matrix proteins was influenced by the coating cell type. Fibroblasts from skin and tendon appeared less capable of preventing the release of matrix proteins than mesenchymal stems cells. Conclusion: The source of cell is an important consideration for cell therapy.

Validation of Relapse Risk Biomarkers for Routine Use in Patients With Juvenile Idiopathic Arthritis

Objective. The myeloid-related proteins 8 and 14 (MRP-8/MRP-14) and neutrophil-derived S100A12 are biomarkers of inflammation. They can be used to determine the relapse risk in patients with juvenile idiopathic arthritis (JIA) after stopping antiinflammatory treatment. In this study, we tested the performance of different enzyme-linked immunosorbent assays (ELISAs) in order to validate systems available for routine use.Methods. MRP-8/MRP-14 and S100A12 serum concentrations of 188 JIA patients in remission were analyzed. Commercially available test systems were compared to experimental ELISAs established in house. The ability of the assays to identify JIA patients at risk for relapse was analyzed.Results. For MRP-8/MRP-14, the PhiCal Calprotectin and Buhlmann MRP8/14 Calprotectin ELISAs revealed hazard ratios of 2.3 and 2.1, respectively. For S100A12, the CircuLex S100A12/EN-RAGE ELISA revealed a hazard ratio of 3.1. The commercial assays allowed a JIA relapse prediction that was at least comparable to the experimental ELISAs.Conclusion. For the prediction of JIA relapse after stopping medication, the biomarkers MRP-8/MRP-14 and S100A12 can be determined by using assays that are available for routine use. The tested commercial MRP-8/MRP-14 and S100A12 ELISAs showed a performance comparable to well-established experimental ELISA protocols when assay-specific cutoffs for the indication of relapse prediction are thoroughly applied.

Comportamento ingestivo de novilhas Nelore em pastejo recebendo suplemento a base de própolis ou monensina sódica

We evaluated the effect of additives the basis of propolis and monensin on ingestive behavior (grazing -GRA, rumination lying -RLY, rumination in foot -RFO, rumination -RUM; idleness lying -ILY, idleness in standing -IDS, idleness -IDL, walking -WAL, posture standing -POS, posture lying -PLY, intake of supplement and water -ISW) of heifers in Tifton 85 grazing. The treatments were: no additive (NAD); propolis - PRO (33.24 mg animal-1 of total flavonoid in apigenin), and monensin -MON (100 mg animal(-1)). Were used five heifers treatment(-1) for evaluation of ingestive behavior during 12 hours of total valuation, which was divided into three periods. Used a factorial design into 3 x 3 for evaluation of ingestive behavior as a function of the evaluation periods. Were utilized three paddocks of 2.9 acres of grass Tifton 85. The additives and mineral supplement (50 g animal(-1)) were provided with 200 g of corn meal to 17 hours. For all activities of the ingestive behaviors observed the effect of period on the time spent on of different activities. The activities of GRA and ISW were higher in the third period (15hours to 19hours) compared to the first period (7hours at 11hours). For IDL and PLY, there was a higher time in minutes for heifers treatment with PRO in the period of 7hours at 11hours, compared to other treatments (NAD and MON). Thus, it is concluded that the use of the additive based on propolis enables sodium monensin replacement, to heifers maintained on pasture without changing eating behavior as well as providing greater idleness during the day the without affecting animal performance.

Marcadores de Microssatélites para estudos de conservação da Raia-viola Rhinobatos percellens (Chondrichthyes, Rhinobatidae)

A exploração pesqueira, tanto artesanal quanto industrial, se constitui na maior ameaça à biodiversidade dos elasmobrânquios. Em escala mundial, o manejo adequado destes recursos é dificultado pela escassez de informações básicas sobre a dinâmica populacional destes organismos. A captura comercial de elasmobrânquios tem alcançado números alarmantes, com a inclusão de diversas espécies nas listas de risco de extinção nacional e também internacional. A identificação e a conservação de estoques geneticamente diferenciados e adaptados ao seu habitat, representam um ponto fundamental para o setor pesqueiro, pela sua relação direta com a produtividade total e o uso sustentável dos recursos. Entre as raias marinhas, a família Rhinobatidae é representada pelos gêneros Rhinobatos, Zapteryx, Trygonorhina e Aptychotrema, sendo registrada a ocorrência da espécie Rhinobatos percellens no Oceano Atlântico adesde as Antilhas e Panamá, Venezuela, Jamaica, Brasil, Uruguai, até a Argentina. No presente trabalho foram desenvolvidos marcadores moleculares do tipo microssatélites para raiaviola, Rhinobatos percellens, para serem utilizados em estudo populacionais. Cinco locus polimórficos foram isolados utilizando um protocolo de enriquecimento. Nos experimentos, foram testados 13 pares de primers, sendo 10 isolados no presente trabalho e 3 isolados para a espécie de tubarão azul (Prionance glauca). Dos primers isolados em Rhinobatos percellens, 5 são polimórficos, 2 monomórficos e 3 apresentaram bandas muito fracas, enquanto os primers testados de tubarão azul mostraram-se monomórficos. Todos os locus estão em equilíbrio de Wardy-Weinberg, exceto o RVA9 (de R. percellens). Os locus polimórficos apresentam de 3 a 6 alelos por locus e heterozigosidade média esperada de 0.62. Considera-se que estes marcadores polimórficos...(Resumo completo, clicar acesso eletrônico abaixo)

DNA human identification for the family rights: one year of agreement between UNESP/laboratory of paternity and public defender service in São Paulo State

The universities have realized the importance of extending their knowledge to the population through the provision of services. Thus, this paper presents the data obtained in an agreement between UNESP/Laboratory of Paternity and Public Defender Service in São Paulo State to make DNA paternity tests.

Adubação nitrogenada para consórcio de alface e rúcula

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

As Tecnologias de Informação e Comunicação TIC na formação de professores em educação sexual: o caso das E-Oficinas na I COES

This article aims to describe how ICT can contribute to the training of teachers on sex education through the lived experience with the implementation of Workshops, which occurred on I COES - I Conference online of sexual education. This Conference was organized by the University of Lisbon-PT, in partnership with UNESP, SP and SC-UDESC. I COES involved education professionals, who work directly in the school to discuss and exchange their experiences related to sexuality education and related fields, through an online space. The research on teacher formation, initial and continuing on sexuality, sex education, gender and sexual diversity, have demonstrated the need to promote and encourage teachers from all areas, to adopt an intentional and emancipatory, their role as sexual educators . Through web conferencing tool Cisco System, I COES was enable the interaction and questioning of ninety five teachers from various parts of Brazil and Portugal, providing opportunities for rich moments of dialogue and reflection on the issues already mentioned, referring to the brazilian and portuguese realities.

Reproduction of Meloidogyne enterolobii in lettuce cultivars of the american group

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Nitric oxide and brazilian propolis combined accelerates tissue repair by modulating cell migration, cytokine production and collagen deposition in experimental leishmaniasis

The fact that drugs currently used in the treatment of Leishmania are highly toxic and associated with acquired resistance has promoted the search for new therapies for treating American tegumentary leishmaniasis (ATL). In this study, BALB/c mice were injected in the hind paw with Leishmania (Leishmania) amazonensis and subsequently treated with a combination of nitric oxide (NO) donor (cis-[Ru(bpy)(2)imN(NO)](PF6)(3)) (Ru-NO), given by intraperitoneal injection, and oral Brazilian propolis for 30 days. Ru-NO reached the center of the lesion and increased the NO level in the injured hind paw without lesion exacerbation. Histological and immunological parameters of chronic inflammation showed that this combined treatment increased the efficacy of macrophages, determined by the decrease in the number of parasitized cells, leading to reduced expression of proinflammatory and tissue damage markers. In addition, these drugs in combination fostered wound healing, enhanced the number of fibroblasts, pro-healing cytokines and induced collagen synthesis at the lesion site. Overall, our findings suggest that the combination of the NO donor Ru-NO and Brazilian propolis alleviates experimental ATL lesions, highlighting a new therapeutic option that can be considered for further in vivo investigations as a candidate for the treatment of cutaneous leishmaniasis.

Projeto Político Pedagógico (PPP): o resgate de sua implantação na rede municipal de ensino de Londrina

The aim of this paper is to present a historical review of the implementation process of the political pedagogical project (PPP) of Londrina (PR). The study used interviews with some agents who participated in the construction of the PPP (teachers, school, supervisors and employees of the Regional Education Center) and investigated the absorption of proposals from the base (school) for the offices of political power. The results showed that although the democratic discourse has always been present in official documents the construction of the PPP had discrete popular participation and projects, as a rule, incorporated the bureaucratic forms issued by official powers.

Pathogenesis of Human and Bovine Cryptosporidium parvum in Gnotobiotic Pigs

To compare the pathogenesis of human genotype 1 (HuGl) and bovine genotype 2 (BoG2) Cryptosporidium parvum, neonatal gnotobiotic pigs were given 1-10 HuGl or BoG2 oocysts. The prepatent and patent periods were significantly longer for HuGl than for BoG2 C. parvum (prepatent, 8.6 vs. 5.6 days; patent, 16.6 vs. 10.3 days). BoG2-infected pigs developed signif- icantly more severe disease than did HuGl-infected pigs. BoG2 parasites were seen micro- scopically throughout the intestines during the prepatent and patent periods. HuGl parasites were only detected during the patent period in the ileum and colon but colonized the mucosal surface in significantly larger numbers than did BoG2. Moderate-to-severe villus/mucosal attenuation with lymphoid hyperplasia was seen throughout the intestines of BoG2-infected pigs, whereas lesions in HuGl-infected pigs were mild to moderate and restricted to the ileum and colon. These findings provide additional support for the hypothesis that human and bovine C. parvum genotypes may be separate species.