Study of the effects of ß-myrcene on rat fertility and general reproductive performance

ß-Myrcene (MYR) is a monoterpene found in the oils of a variety of aromatic plants including lemongrass, verbena, hop, bay, and others. MYR and essential oils containing this terpenoid compound are used in cosmetics, household products, and as flavoring food additives. This study was undertaken to investigate the effects of MYR on fertility and general reproductive performance in the rat. MYR (0, 100, 300 and 500 mg/kg) in peanut oil was given by gavage to male Wistar rats (15 per dose group) for 91 days prior to mating and during the mating period, as well as to females (45 per dose group) continuously for 21 days before mating, during mating and pregnancy, and throughout the period of lactation up to postnatal day 21. On day 21 of pregnancy one-third of the females of each group were submitted to cesarean section. Resorption, implantation, as well as dead and live fetuses were counted. All fetuses were examined for external malformations, weighed, and cleared and stained with Alizarin Red S for skeleton evaluation. The remaining dams were allowed to give birth to their offspring. The progeny was examined at birth and subsequently up to postnatal day 21. Mortality, weight gain and physical signs of postnatal development were evaluated. Except for an increase in liver and kidney weights, no other sign of toxicity was noted in male and female rats exposed to MYR. MYR did not affect the mating index (proportion of females impregnated by males) or the pregnancy index (ratio of pregnant to sperm-positive females). No sign of maternal toxicity and no increase in externally visible malformations were observed at any dose level. Only at the highest dose tested (500 mg/kg) did MYR induce an increase in the resorption rate and a higher frequency of fetal skeleton anomalies. No adverse effect of MYR on postnatal weight gain was noted but days of appearance of primary coat, incisor eruption and eye opening were slightly delayed in the exposed offspring. On the basis of the data presented in this paper the no-observed-adverse-effect level (NOAEL) for toxic effects on fertility and general reproductive performance can be set at 300 mg of ß-myrcene/kg body weight by the oral route.

The leaves of green plants as well as a cyanobacterium, a red alga, and fungi contain insulin-like antigens

We report the detection of insulin-like antigens in a large range of species utilizing a modified ELISA plate assay and Western blotting. We tested the leaves or aerial parts of species of Rhodophyta (red alga), Bryophyta (mosses), Psilophyta (whisk ferns), Lycopodophyta (club mosses), Sphenopsida (horsetails), gymnosperms, and angiosperms, including monocots and dicots. We also studied species of fungi and a cyanobacterium, Spirulina maxima. The wide distribution of insulin-like antigens, which in some cases present the same electrophoretic mobility as bovine insulin, together with results recently published by us on the amino acid sequence of an insulin isolated from the seed coat of jack bean (Canavalia ensiformis) and from the developing fruits of cowpea (Vigna unguiculata), suggests that pathways depending on this hormone have been conserved through evolution.

Nåden och gåvan i Philipp Melanchthons rättfärdiggörelselära 1521-1535

In this thesis Philip Melanchthon’s doctrine of justification is analyzed, especially the aspects of favor and donum in his writings until 1535. The research task is defined in the introduction of the thesis. Researchers agree, that Melanchthon emphasized the forensic aspect of the doctrine of justification, but this is not the case with the effective aspect of his doctrine of justification. Therefore, I have chosen to focus on the concepts of favor (grace) and donum (gift). The thesis begins with an analysis of the first edition of the Loci communes of 1521 in chapter two, and it ends with the second edition from 1535 in chapter seven. Melanchthon’s most important writings from the intervening period are analyzed in chapters three to six. In chapter eight, I describe my final results from the analysis. Although I have focused on how Melanchthon perceived the relationship between favor and donum, I have also taken note of such loci that are associated with his view of justification. I have done this in order to get a clearer picture of Melanchthon’s doctrine of justification, and of the issues which change in his theological thinking over time. In the research overview in chapter one, I divided the researchers in four different groups depending on how they perceive Melanchthon’s doctrine of justification. This classification is of course not comprehensive, since the reality is much more complicated. The main interpretations present in Melanchthon research are: 1. Christ as favor in justification, but donum is excluded from justification. 2. Christ as favor in justification, but the Spirit is donum in justification. 3. Christ as both favor and donum in justification until the early 1530s. 4. Christ as both favor and donum in justification throughout his life. The fourth option is in my opinion the most preferable. The analysis of Melanchthon’s writings shows that he expects both a forensic, and an effective aspect in the doctrine of justification throughout the examined period. Melanchthon strongly emphasizes that justification is by grace alone through faith for Christ’s sake, but also that the Holy Spirit works the justifying faith, and is the gift bestowed on believers in connection with this. Where the opponents according to Melanchthon see deeds, virtues, or a quality in the believer as the cause of the justification, there Melanchthon sees only Christ.

Heterofucans from Dictyota menstrualis have anticoagulant activity

Fucan is a term used to denote a family of sulfated L-fucose-rich polysaccharides which are present in the extracellular matrix of brown seaweed and in the egg jelly coat of sea urchins. Plant fucans have several biological activities, including anticoagulant and antithrombotic, related to the structural and chemical composition of polysaccharides. We have extracted sulfated polysaccharides from the brown seaweed Dictyota menstrualis by proteolytic digestion, followed by separation into 5 fractions by sequential acetone precipitation. Gel electrophoresis using 0.05 M 1,3-diaminopropane-acetate buffer, pH 9.0, stained with 0.1% toluidine blue, showed the presence of sulfated polysaccharides in all fractions. The chemical analyses demonstrated that all fractions are composed mainly of fucose, xylose, galactose, uronic acid, and sulfate. The anticoagulant activity of these heterofucans was determined by activated partial thromboplastin time (APTT) using citrate normal human plasma. Only the fucans F1.0v and F1.5v showed anticoagulant activity. To prolong the coagulation time to double the baseline value in the APTT, the required concentration of fucan F1.0v (20 µg/ml) was only 4.88-fold higher than that of the low molecular weight heparin Clexane® (4.1 µg/ml), whereas 80 µg/ml fucan 1.5 was needed to obtain the same effect. For both fucans this effect was abolished by desulfation. These polymers are composed of fucose, xylose, uronic acid, galactose, and sulfate at molar ratios of 1.0:0.8:0.7:0.8:0.4 and 1.0:0.3:0.4:1.5:1.3, respectively. This is the fist report indicating the presence of a heterofucan with higher anticoagulant activity from brown seaweed.

Livets källa kärleken : hälsans urkraft

This basic research focuses on the ethos of health and the human being's becoming in health. The theoretical perspective consists of the caring tradition within caring science developed at Åbo Akademy University. The aim of the present doctoral thesis is to uncover a new understanding as well as to deepen and attain a more nuanced understanding of the ethos of health, the essence of health, by penetrating to the core of what gives the human being the strength for experiencing a becoming in health. The research questions are as follows: l) What is the human being's source of strength? and 2) What reveals the source of strength so that the human being can perceive it and dedicate its strength in order to experience a becoming in health? The primary methodology used in the dissertation is hermeneutical. The material consists of the work Kärlekens gerningar by Kierkegaard, texts from focused interviews with respondents who have lived through severe personal suffering, as well as the book Det bländande mörkret by Wikström. These texts are interpreted through hermeneutical reading. The new horizon of understanding that emerges is reflected towards Eriksson's caritative theory, towards prior research within the tradition of caring science at Åbo Akademy University and towards previous national and international studies within this field. The new understanding shows that the human being's source of strength is love, the essence and origin of life. The substance of health is love, which, through the trinity of faith, hope and love, also makes possible the existence of the source of strength. Love has a deeper dignity than faith and hope, is connected with eternity and is the uniting link between temporality and eternity. The human being's inner longing entails an ontological attraction towards the source of strength. This source of strength is hidden, which provides and maintains its force, like a mystery connected with the darkness of suffering that hides the secret representing the source of strength, life's mystery, bu t w hi ch is revealed in both the darkness of suffering and in the light of joy. The dedication of strength requires freedom, willingness and courage to see the light, despite awareness of shame and guilt. Creative acts liberate the human being for the dedication of strength, which is preceded by a holy presence where, in solitude, the human being makes sacrifices for the sake of his or her human smallness and weakness, and allows himself or herself to be enclosed by the darkness of suffering to discover the light from the source. This entails being enraptured in a quiet "doing" in order to experience the beauty that bears witness to the holy which creates unity. The source of strength is revealed through beauty. The ethos of the human being and the ethos of health have the same fundamental substance, whilst the ethos of life possesses the deepest dimension and concerns the mysterious and infinite eternity. The ethos of life, eternity, which is a wellspring of strength, is not in itself strength-giving unless it is allied with love. Health can be understood in the light of life, of which death is an inevitable part. Life itself constitutes and creates the source which, through its alliance with eternity' s primordial wellspring of strength, generates strength from which the human being's source of strength, love, receives its eternal fervour. The human being is fundamentally interconnected with an abstract other, the first love, a universal wellspring of strength. Through Communion with this abstract other a dedication of the strength to experience a becoming in health becomes possible. Love for one's neighbour is the fundamental substance in the movement of becoming in health. Becoming in health presupposes a simultaneous movement in which the human being practices the human calling through ethos. As one loves one's neighbour through actions the still forces of eternity are in motion. When life emerges in the foreground and becomes the home of the human being, a dedication of the power of love is possible. Life itself determines the human being's becoming in health. A humble fundamental attitude towards life constitutes the basis for a continuous dedication of vitality from this source.

A carbohydrate-based mechanism of species recognition in sea urchin fertilization

In the present review, we describe a systematic study of the sulfated polysaccharides from marine invertebrates, which led to the discovery of a carbohydrate-based mechanism of sperm-egg recognition during sea urchin fertilization. We have described unique polymers present in these organisms, especially sulfated fucose-rich compounds found in the egg jelly coat of sea urchins. The polysaccharides have simple, linear structures consisting of repeating units of oligosaccharides. They differ among the various species of sea urchins in specific patterns of sulfation and/or position of the glycosidic linkage within their repeating units. These polysaccharides show species specificity in inducing the acrosome reaction in sea urchin sperm, providing a clear-cut example of a signal transduction event regulated by sulfated polysaccharides. This distinct carbohydrate-mediated mechanism of sperm-egg recognition coexists with the bindin-protein system. Possibly, the genes involved in the biosynthesis of these sulfated fucans did not evolve in concordance with evolutionary distance but underwent a dramatic change near the tip of the Strongylocentrotid tree. Overall, we established a direct causal link between the molecular structure of a sulfated polysaccharide and a cellular physiological event - the induction of the sperm acrosome reaction in sea urchins. Small structural changes modulate an entire system of sperm-egg recognition and species-specific fertilization in sea urchins. We demonstrated that sulfated polysaccharides - in addition to their known function in cell proliferation, development, coagulation, and viral infection - mediate fertilization, and respond to evolutionary mechanisms that lead to species diversity.

Distribution of human immunodeficiency virus type 1 subtypes in the state of Amazonas, Brazil, and subtype C identification

Few studies have reported the molecular epidemiological characterization of HIV-1 in the Northern region of Brazil. The present study reports the molecular and epidemiological characterization of 31 HIV-1 isolates from blood donors from the State of Amazonas who donated blood between April 2006 and March 2007. Serum/plasma samples from all donors were screened for HIV antibodies by ELISA and the results confirmed by Western blot analysis. Genomic DNA was extracted from the buffy coat using the Super Quik-Gene-DNA Isolation kit. Nested PCR was performed on the env, gag, and pol regions of HIV-1 using the Gene Amp PCR System 9700. Sequencing reactions were performed using the inner PCR primers and the DYEnamic™ ET Dye Terminator Kit, and phylogenetic analysis was performed using the gag, pol, and env gene sequences. We collected samples from 31 blood donors who tested positive for HIV-1 in confirmatory experiments. The male:female ratio of blood donors was 3.4:1, and the mean age was 32.4 years (range: 19 to 61 years). Phylogenetic analysis showed that subtype B is the most prevalent among Northern Brazilian HIV-1-seropositive blood donors. One HIV-1 subtype C and one circulating recombinant form (CRF_BF) of HIV-1 were identified in the State of Amazonas. This is the first study showing the occurrence of a possible "homogenous" subtype C in this region of Brazil. This finding could contribute to a better characterization of the HIV-1 strains that circulate in the country.

LENTIL TANNIN-GLOBULIN INTERACTIONAND AND IN VITRO HYDROLYSIS

Protein fractions were isolated from lentil cotyledons and tannins were isolated and purified from lentil seed coats. The globulin fraction corresponded to 42.7% of the total lentil flour nitrogen, representing the major protein fraction. Acetone:water (7:3) was the best extractant for seed coat tannins compared to methanol or methanol-HCl 1%. Native and heated (99oC/15 min.) isolated lentil globulin and casein were hydrolyzed with trypsin and pepsin in the absence of tannins and at 1:40, 1:20, 1:10, 1:5 and 1:2.5 tannin-to-protein ratios. The tryptic and peptic hydrolysis of the unheated proteins were reduced with increasing tannin-to-protein ratios. Unheated casein showed to be more susceptible to trypsin than globulin and the opposite effect was observed with pepsin. Heating followed by tannin interaction and hydrolysis had a more pronounced effect on tryptic than peptic digestion for both proteins.

Effect of ambient conditions on dimensional stability and stiffness of commercial paperboard and paperboard trays

The objective of this study was to develop laboratory test methods for characterizing the effects of changed moisture content on paperboard trays produced by press-forming process. Influence of moisture on the properties of unconverted paperboard such as bending stiffness, bursting strength, and curling was studied. Paperboard and tray samples were tested after storing in different relative humidity conditions (35, 50, 65, 80 and 95% RH). The effect of PE and PET extrusion coatings on these properties was also studied. It was found that increase in moisture content of paperboard decreases bending and bursting strength, dimensional stability and stiffness of paperboard trays. Such physical and mechanical properties as bending stiffness and curling of paperboard seem to define the stiffness of ready-made trays and their dimensional stability. Paperboards and trays with extruded PE and PET one sided coatings demonstrated higher strength properties but at the same time had lower dimensional stability comparing to uncoated paperboards. Samples with smaller polymer coat weight had better dimensional stability than respective samples with higher coat weight. It was also found that preconditioning of paperboard in lower humidity environment before press-forming could improve dimensional stability and stiffness of ready-made tray.

Jöns Budde och hans skrifter – yngre fornsvenskt textmaterial och mannen bakom det

Tutkielman aiheena on kieli ja tyyli Jöns Budden latinasta muinaisruotsiksi kääntämissä teksteissä. Tarkoituksena on tutkia, millaisia tekstejä Budde käänsi ja missä tarkoituksessa. Lisäksi tutkin kielikontakteja latinaan ja saksaan sekä Buddea henkilönä hänen tuotantonsa valossa. Arvioin myös aiempien Buddeen liittyvien hypoteesien paikkansapitävyyttä. Materiaali käsittää kaikki tekstit, joiden yleisesti oletetaan olevan Budden kääntämiä. Osa on anonyymejä, mutta niiden yhteys Buddeen on erittäin todennäköinen. Aineisto käsittää noin 1350 sivua painetuissa editioissa. Tutkimus on materiaalilähtöistä: tarkan tekstinluvun ja teksti-interpretaation kautta olen kiinnittänyt huomioni erilaisiin etupäässä tyylillisiin seikkoihin, joita kommentoin ottaen huomioon kulttuurihistoriallisen kontekstin. Tutkimus kohdistuu kielenkäyttöön tietyssä konkreettisessa materiaalissa eli tarkastelun kohteina ovat parole ja synkronia ensisijaisemmin kuin diakronia ja langue eli kieli systeeminä ja resurssina. Metodina on siten filologinen teksti-interpretaatio, ja tutkimus on kommentaari Budden teksteihin. Keskeinen tulos on, että Budde on tietoinen stilisti, joka käyttää kieliopin ja stilistiikan keinoja sisällöllisten asioiden painottamiseen ja dramatisointiin. Budden tyyli-ihanteilla on suureksi osaksi esikuvia latinassa, mutta Budde ei noudata niitä orjallisen sokeasti, vaan on itsenäinen alkuteksteihin nähden. Toinen merkittävä tulos liittyy Budden kääntämien Raamatun kirjojen tarkoitukseen. Käsitykseni mukaan niitä ei ole tarkoitettu luettaviksi sellaisenaan, vaan apuvälineiksi luettaessa varsinaista pyhää kirjaa eli Vulgataa latinaksi. Tekstien tekijyyteen liittyvät aiemmat hypoteesit saavat tukea tutkimuksestani, kun taas Budden aristokraattisiin yhteyksiin ja hänen mahdollisiin opintoihinsa Saksassa liittyvät hypoteesit osoittautuvat liian rohkeiksi ottaen huomioon säilyneiden lähteiden niukkuuden.

Physicochemical and antioxidative properties of black, brown and red rice varieties of northern Thailand

Rice, the seed of Oryza species, is the major cereal crop in most of the developing countries. Nearly 95% of global rice production is done in Asian countries, and about half of the world’s population consumes it. Some speciality rices are not commonly consumed. Colored rice is one of such variety. In these varieties, high amounts of anthocyanin pigment are deposited in the rice coat to form its black (also known as purple), brown and red colors. Minimum studies are there to explain the properties of these rice varieties of Thailand. Thus, the current study was aimed to assess the physicochemical and antioxidative properties of three rice varieties (Chiang Mai Black rice, Mali Red rice and Suphanburi-1 Brown rice) of different cultivars of northern Thailand. Rice bran extracts of these three cultivars were prepared with different solvents (polar and non-polar) for the evaluation of total phytochemical content and anti-oxidant free-radical-scavenging properties. Chiang Mai Black rice contained higher concentration of phenolic acid, flavonoids, and anthocyanins (Cyanidin 3-glucoside, peonidin 3-glucoside, cyanidin chloride). Chiang Mai Black rice is richer in free-radical-scavenging compounds and activities than the other tested varieties. Polar extractions of rice bran are high in anti-oxidative compounds and activities than non-polar extractions.

Modificações na condição física das sementes de cenoura em equipamento de recobrimento

O objetivo do trabalho foi avaliar equipamento desenvolvido para o recobrimento de sementes e seu efeito na qualidade física de sementes de cenoura em função da aderência de diversos materiais inertes em diferentes proporções: aglomerante (vermiculita) nas proporções 2:1 e 3:1; adesivo (acetato de polivinil) e o polímero + corante "Color coat"®, bem como a adição do fungicida Thiram + Carbendazin (250ml para cada 100kg de semente). Foram obtidas informações quanto à modificação do tamanho, forma, peso e teor de água das sementes da cultivar Tropical. Mediante a utilização de peneiras detectou-se que as sementes mantiveram-se individualizadas, com modificação de tamanho, peso e forma. Os resultados obtidos permitiram concluir que o equipamento de recobrimento de sementes mostra-se funcional na aderência das diferentes camadas e o polímero associado ao corante fornece aparência satisfatória às sementes de cenoura recobertas; o equipamento de recobrimento de sementes é eficiente na modificação do tamanho, forma e do peso das sementes de cenoura; o acetato de polivinil é eficiente adesivo no recobrimento de sementes de cenoura e as sementes de cenoura absorvem água após o recobrimento, devendo sofrer secagem artificial antes do armazenamento.

Recobrimento de sementes de cenoura com aglomerante em diversas proporções e fungicida

O objetivo do trabalho foi avaliar a qualidade fisiológica de sementes de cenoura após o recobrimento com aglomerante (vermiculita) nas proporções 2:1 e 3:1 e a adição do fungicida Thiram + Carbendazin. Também foram utilizados adesivo (acetato de polivinil) e corante "color coat". A qualidade fisiológica das sementes de cenoura (Daucus carota L.), cv. Tropical, recobertas foi avaliada pelos testes de germinação, primeira contagem da germinação, envelhecimento acelerado, teste de frio, velocidade e porcentagem de emergência em casa de vegetação e em campo. O recobrimento de sementes de cenoura com fungicida Thiram 600g + Carbendazin 200g, com uma concentração de 0,25% (250mL de fungicida para cada 100kg de semente), aglomerante na proporção 3:1 e corante color coat não afeta a qualidade fisiológica das sementes. O recobrimento de sementes de cenoura aumenta o teor de água das sementes em aproximadamente 16 pontos percentuais, por isso devem ser secadas imediatamente.

Methods for overcoming dormancy in Dinizia excelsa Ducke seeds

The impermeability of seed coat to water is common mechanism in Fabaceae seeds. Treatments to overcome hardseededness include scarification with sulphuric acid, scarification on abrasive surface and soaking in water among others. The objective of this study was to identify an effective method to overcome dormancy in Dinizia excelsa seeds. A pre-test (untreated seed) and three experiments were carried out: immersion of seeds in acid sulphuric for 10, 20, 30, 40, 50 and 60min (experiment 1); scarification on abrasive surface at the positions distal end, near of the mycrophyle and on the lateral tissue and tegument clipping at 1mm of the distal end, near of the mycrophyle and on the lateral tissue (experiment 2); scarification on abrasive surface and immersion in water for 0, 12, 24 and 48h (experiment 3). The experimental design was completely with four replications of 50 seeds for each treatment. The statistical analysis was carried out by ANOVA and regression analysis. Seedlings emergence on untreated seeds started on the 8th day after sowing and reached 52.5% on the 1,709th day. In general, the treatments to overcome dormancy increase emergence. Emergence was higher for seeds treated with sulphuric acid for 20 and 30min with emergence of 93.6% and 86.6%, respectively. For seeds scarified on abrasive surface higher emergences were recorded for scarification on distal end, near of the mycrophyle and on the lateral, 82.7%, 74.3% and 75.7%, respectively. Seeds scarified manually showed higher emergence when not immersed in water (75%), or when immersed for 12 and 24h (75%, 73.6% and 65.6%, respectively). Immersion seeds in sulphuric acid for 20 and 30min and scarification on abrasive surface of distal end are effective to overcome dormancy in D. excelsa.

Physiological quality of soybean and wheat seeds produced with alternative potassium sources

The use of unconventional sources of K for plants has been widely studied, but the effects of alternative materials on physiological seed quality are still relatively unknown. The objective of this study was to evaluate the physiological quality of soybean and wheat seeds after using different potassium sources in a crop succession. The experimental design was a completely randomized block with four replications. Treatments consisted of three K sources (KCl, alkaline rock and ground phonolite, with 58%, 11% and 8.42% of K2O, respectively) applied in four doses (0, 25, 50 and 100 kg K2O ha-1). Potassium doses were applied in soybean and their residual effects were evaluated on the following wheat crop. Soybean and wheat seeds were evaluated immediately after harvesting by tests for moisture content, seed weight, germination, first count, electrical conductivity, seedling length and seedling dry matter. Soybean plants fertilized with alternative sources of K produced heavier seeds with a lower coat permeability compared to KCl; the physiological quality of soybean seeds and the weight of wheat seeds increase due to higher K2O doses, independently of their source.