Prolonged but not short negative energy condition restored corticoadrenal leptin sensitivity in the hypothalamic obese rat.

BACKGROUND/AIM: We have reported that neonatal treatment with monosodium L-glutamate (MSG), which causes damage to the arcuate nucleus, leads to severe hyperleptinemia and reduced adrenal leptin receptor (ob-Rb) expression in adulthood. As a result, rats given MSG neonatally display corticoadrenal leptin-resistance, a defect that is overridden by normalization of corticoadrenal hyperfunction. The aim of the present study was to determine whether negative energy conditions could correct corticoadrenal cell dysfunction in rats given MSG neonatally. METHODS: Normal (CTR) and MSG-treated female rats were subjected to food removal for 1-5 days, or prolonged (24-61 days) food restriction (FR). Plasma levels of several biomarkers and in vitro corticoadrenal function were evaluated following starvation or FR. RESULTS: Fasting for 1-5 days reduced plasma leptin levels in CTR and MSG rats, compared to levels in the respective groups fed ad libitum(p < 0.05), but adrenal leptin-resistance was unchanged. With prolonged FR, isolated adrenal cells from MSG rats became sensitive to leptin, which lowered ACTH-induced glucocorticoid release. This restoration of leptin response was associated with normalization of adrenal ob-Rb gene expression. CONCLUSION: Dietary restriction in some leptin-resistant obese phenotypes may normalize adrenocortical function.

08005-002 Silver Lake Watershed Final Report

Silver Lake is located in an 18,053-acre watershed. The watershed is intensively farmed with almost all of the wetlands being previously drained or degraded over the last 50 years. Silver Lake is listed on the State of Iowa’s impaired water bodies list due to sediment and high nutrient level. Silver Lake is also known be in the bottom 25 percentile of Iowa’s lakes due Secchi disk readings and Chlorophyll a level. Farming in the watershed is the principle concern and cause for many of the problems occurring in Silver Lake currently with 78% of the watershed being intensively farmed. There are two major drainage ditches that have been used to drain the major wetlands and sloughs that, at one time, filtered the water and slowed it down before it reached Silver Lake. With these two major drainage ditches, water is able to reach the lake much faster and unfiltered than it once did historically. The loss of 255 restorable wetland basins to row crop production has caused serious problems in Silver Lake. These wetland basins once slowed and filtered water as it moved through the watershed. With their loss over the last 50 years that traditional drainage no longer occurs. We propose to create a Wetland Reserve Program incentive project to make WRP a more attractive option to landowners within the watershed. The incentive will be based on the amount of sediment delivery reduction to the lake, therefore paying a greater payment for a greater benefit to the lake. The expected result of this project is the restoration of over 250 acres of wetland basins with an associated 650 acres of upland buffers. The benefit for these wetlands and buffers would be reduced sediment, reduced nutrients, and slowed waters to the lake.

1210-007IJ Central Park Lake Final Report

The Central Park Lake Watershed Assessment and Management Plan identified four categories where improvements are needed to remove the 23 acre lake from the impaired waters list. These include the wastewater system, runoff from surrounding lands, in-lake nutrient re-suspension and runoff from hard surfaces within the park. The lake is currently impaired for bacteria, algae and pH. Through outcomes of the Watershed Assessment and Management Plan, this proposal includes for abandonment and reclamation of the single cell wastewater lagoon site, replacement with three conventional septic systems and construction of two wetlands. One of the wetlands is located on the same site as the reclaimed lagoon and the other is located to intercept sediment and trap nutrients transported by tile lines. The prescribed wastewater system improvements are based on assessment by grab samples test by the State Hygienic Lab, development of a Preliminary Engineering Report, soil analysis and communication with IDNR wastewater officials. The two wetland sites were assessed by officials from IDALS and the Jones County SWCD. This project is part of $1.7 million lake restoration effort to reclaim the 47 year old lake. The lake has a positive economic impact of more than $7.6 million annually and supports an average annual visitation of 58,145, according to the Iowa Lakes Valuation Project, conducted by Iowa State University.

08009-005 Storm Lake Watershed Final Report

Storm Lake, the state's fourth largest natural lake, has been the centerpiece of significant economic development, including a resort, water park, and state marina. While there have been considerable improvements to the lake's water quality through a major on-going lake restoration program and watershed project, the Little Storm Lake area still needs to be addressed. Little Storm Lake is a 190 acre area on the nmihwest side of Storm Lake. The water level in both areas is based on the dam height located on the southeast corner of Storm Lake. Approximately 70% of the water from the watershed flows through Little Storm Lake. Little Storm Lake originally had the ability to remove much of the sediment and nutrients from incoming waters. However, due to degradation, proper wetland function has been compromised. Under normal hydrologic conditions Little Storm Lake has the potential to function as a sediment trap for Storm Lake, but tllis capacity is overwhelmed during high flows. Little Storm Lake is at or near its sediment trapping capacity, which results in higher sediment transport into Storm Lake. Resuspension of sediments due to wind and other in-lake dynamics, such as rough fish, further exacerbate the total turbidity from suspended sediment and results in movement of sediment from Little Storm Lake into Storm Lake. This project includes a fish barrier and water retention structure between Little Storm Lake and Storm Lake and the construction of a pumping station and associated equipment. The project involves periodic dewatering of Little Storm Lake during years of favorable climatological conditions to consolidate the sediments and revegetate the area. Construction of the fish banier would aid restoration efforts by preventing rough fish from destroying the vegetation and would decrease recruitment of rough fish by limiting their spawning area. In the future, if the diminished trapping capacity of Little Storm Lake still results in sediment moving into Storm Lake, a dredging project would be initiated to deepen Little Storm Lake.

1109-003 Coe Creek Watershed Final Report

The city of Elliott has had an increase in nitrate levels in their community water supply located in the Coe Creek Watershed. They have been working with the IDNR Source Water Protection (SWP) Programs to conduct site investigations and have formed a SWP Planning Team. This Team has been reviewing the investigation findings, formed an action plan and studied different Best Management Practices (BMPs). After considering the BMPs the SWP Team made a recommendation to the Elliott City Council which included native grass seeding and a shallow water wetland. The Team also held an informational meeting for the citizens of Elliott. The goal of this meeting was to inform and educate the public of the Team findings and BMPs. The Elliott City Council approved the restoration of a shallow wetland with a native grass buffer. This whole project is 27 acres and includes a shallow water wetland with native grass buffer. This would be a long term method to reduce nitrates in the city wells. Elliott is partnering with the Natural Resources Conservation Service, Montgomery County Soil and Water Conservation District, Pheasants Forever, the Montgomery County Conservation Board, US Fish and Wildlife Service and the Montgomery County Board of Supervisors in the restoration of the shallow water wetland and native grass buffer.

1009-004 Lost Island Lake Watershed Final Report

The Lost Island Lake watershed is located in the prairie pothole region, a region dotted with glacial wetlands and shallow lakes. At 1,180 acres, Lost Island Lake is the state's fifth largest natural lake and its watershed is comprised of nearly 1,000 acres of wetland habitat, including Iowa 's largest natural wetland – Barringer Slough. Unfortunately, Lost Island and its associated wetlands are not functioning to their fullest ecological and water quality potential. In 2002 and 2004, Lost Island Lake was categorized as '·impaired'" on Iowa's Impaired Waters List. Frequent algal blooms and suspended solids drastically increase turbidity levels resulting in its impairment. To investigate these concerns, a two-year study and resulting Water Quality Improvement Plan were completed. The water quality study identified an overabundance of non-native common carp (Cyprinus carpio) in the lake and its surrounding wetlands as a primary cause of impairment. The goal of the Lost Island Lake Watershed Enhancement Project is to restore ecological health to Lost Island Lake and its intricate watershed resulting in improved water quality and a diverse native plant and wildlife community. The purpose of this grant is to obtain funding for the construction of two combination fish barriers and water control structures placed at key locations in the watershed within the Blue Wing Marsh complex. Construction of the fish barriers and water control structures would aid restoration efforts by preventing spawning common carp from entering wetlands in the watershed and establishing the ability to manage water levels in large wetland areas. Water level management is crucial in wetland health and exotic fish control. These two structures are part of a larger construction project that involves a total of four combination fish barriers and water control structures and one additional fish barrier. The entire Lost Island Lake Watershed Enhancement Project is a multi-year project, but the construction phase for the fish barriers and water control structures will be completed before December 31, 2011.

07040-015 Saylor Creek Watershed Final Report

Phase 2 of the Saylor Creek Improvement Project is focused on channel restoration. The existing stream channel is generally incised, running through areas primarily covered with heavy trees, brush and weeds. The ravine ranges from 6 to 20 feet deep through the corridor with very steep slopes in several areas. In two areas storm outlets are undercut or suspended above the channel. Tall undercut, eroded banks exist along several of the outside bends. Sediment deposition on the inside bends limits the cross-section of the channel, increasing flow velocity and forcing this faster flow toward the eroded outside bank. A wide array of practices will need to be implemented to address channel erosion. Improvements will be specifically tailored to address problems observed at each bend. The result will be a channel with a more natural appearance, and reduced use of hard armor and revetment. Some sections will require minimal grading with removal of underbrush for improved maintenance access and more sun exposure, better allowing deep­ rooted plants and flowers to establish to provide further erosion protection. Straight sections with steep banks will require grading to pull back slopes, increasing the creek's capacity to convey storm flows at slower velocities. Sections with sharp bends will require slope pull back and armor protection. A constructed wetland will collect and treat runoff from a small sub-watershed, before being discharged into the main tributary.

06017-009 Saylor Creek Watershed Final Report

With the Saylor Creek Watershed Improvement Project, Iowa Heartland RC&D and other area stakeholders have an opportunity to display how "best management practices" (BMPs) can reduce storm water runoff and improve the quality of that runoff in an urban setting. Conservation design is a uew approach to storm water management that addresses the negative impacts of storm water runoff and turns them into a positive. The master plan for the Prairie Trail development surrounding the watershed project will incorporate bioretention cells, bioswales, buffer strips, rain gardens, as well as native plant landscaping to slow storm water runoff and naturally clean sediment out of the water before it reaches Saylor Creek. In addition to conservation design elements, the project will utilize storm water detention ponds and creek bed restoration to develop a complete storm water "treatment train" system within Prairie Trail. The extensive use of conservation storm water management for Prairie Trail is unique for urban development in Iowa.

1214-008 Clear Lake Watershed Final Report

Clear Lake, Iowa's third largest natural lake, is a premier natural resource and popular recreational destination in north central Iowa. Despite the lake's already strong recreational use, water quality concerns have not allowed the lake to reach its full potential. Clear Lake is listed on Iowa's Draft 2010 303(d) Impaired Waters List for algae, bacteria, and turbidity. Many restoration practices have been implemented to treat the algae and turbidity impairment, but few practices have been installed to treat bacteria. Reducing beach bacteria levels is a priority of the lake restoration partners. Federal, State, and local partners have invested more than $20 million in lake and watershed restoration efforts to improve water clarity and quality. These partners have a strong desire to ensure high bacteria levels at public swim beaches do not undermine the other water quality improvements. Recent bacteria source tracking completed by the State Hygienic Laboratory indicates that Canada Geese are a major contributor of bacteria loading to the Clear Lake swim beaches. Other potential sources include unpermitted septic systems in the watershed. The grant request proposes to reduce bacteria levels at Clear Lake's three public swim beaches by utilizing beach cleaner machines to remove goose waste, installing goose deterrents at the swim beaches, and continuing a septic system update grant program. These practices began to be implemented in 2011 and recent bacteria samples in 2012 are showing they can be effective if the effort is continued.

1228-013 Center Lake Watershed Final Report

A targeted approach is being used in the Iowa Great Lakes Watershed with a keystone project featured within this project application in the heavily urbanized Center Lake Watershed. As identified in the Iowa Great Lakes Watershed Management Plan, urban runoff is the only remaining watershed concern in the Center Lake Watershed as the map in the attachments clearly shows. Fully one third of the watershed concerns of Center Lake will be treated through the installation of 7 keystone urban practices and will remove 63 pounds of phosphorous from entering the lake annually. Due to the interconnectedness of the Iowa Great Lakes (IGL), the watershed has been broken down into sub units called Resource Management Areas (RMA's) for priority practice implementation. This project will mesh with the existing Iowa Great Lakes Watershed Management Plan by reducing pollutant loads from the highest priority RMA's which are resulting in impaired water bodies. The majority of the funding needed for the specific practices specified in this proposal has already been secured through the Iowa DNR Section 319 and Lake Restoration Programs, The Water Quality Commission and the City of Spirit Lake. This funding request will simply bring the overall cost of these keystone practices into the range of affordability for the committed funders and the City of Spirit Lake

Potential Role of TRAIL in the Management of Autoimmune Diabetes Mellitus.

Type 1 diabetes mellitus (T1DM) is an autoimmune disease, due to the immune-mediated destruction of pancreatic β-cells, whose incidence has been steadily increasing during the last decades. Insulin replacement therapy can treat T1DM, which, however, is still associated with substantial morbidity and mortality. For this reason, great effort is being put into developing strategies that could eventually prevent and/or cure this disease. These strategies are mainly focused on blocking the immune system from attacking β-cells together with functional islet restoration either by regeneration or transplantation. Recent experimental evidences suggest that TNFrelated apoptosis-inducing ligand (TRAIL), which is an immune system modulator protein, could represent an interesting candidate for the cure for T1DM and/or its complications. Here we review the evidences on the potential role of TRAIL in the management of T1DM.

Goya's artwork imaging with Terahertz waves

In this paper we use a Terahertz (THz) time-domain system to image and analyze the structure of an artwork attributed to the Spanish artist Goya painted in 1771. The THz images show features that cannot be seen with optical inspection and complement data obtained with X-ray imaging that provide evidence of its authenticity, which is validated by other independent studies. For instance, a feature with a strong resemblance with one of Goya"s known signatures is seen in the THz images. In particular, this paper demonstrates the potential of THz imaging as a complementary technique along with X-ray for the verification and authentication of artwork pieces through the detection of features that remain hidden to optical inspection.

El deterioro del soporte de contrachapado: propuesta de restauración de 9 obras de Francesc Artigau

Francesc Artigau (Barcelona 1940) pintó en los años setenta una serie de obras al temple sobre contrachapado. De éstas, dos trípticos y un díptico estaban destinados a decorar un hotel en Masquefa (Lérida), pero fueron rechazados por su atrevimiento y sus colores, y quedaron en propiedad del agente intermediario, junto con otra obra de menor formato. Las nueve obras sufrieron después un grave proceso de deterioro causado por una conservación en condiciones deplorables, al estar almacenadas en un local en el que se guardaba un grupo indeterminado de perros. Las obras, actualmente propiedad del Museo de l'Hospitalet (Barcelona), han permanecido fuera del circuito artístico hasta la actualidad. Se exponen los primeros resultados de la fase de estudio del soporte de las nueve obras y las propuestas de intervención en los soportes dañados

Cambios de color y pH causados por el PVAc en bienes culturales

Este artículo presenta los primeros resultados del estudio "Identificación de patologías causadas por el PVAc en bienes culturales" que se está realizando en la Sección de Conservación-Restauración de la Facultad de Bellas Artes, Universidad de Barcelona. El trabajo se basa en el estudio de obras originales que fueron tratadas con PVAc en los años 70-80 y pretende identificar los problemas que genera el PVAc en materiales de archivo, arqueológicos, pintura sobre tela, sobre madera y pintura mural. Tras el análisis de las obras originales, se han preparado muestras probeta que reproducen sus características, así como de adhesivos de PVAc comerciales y de uso específico en restauración. Éstas han sido analizadas antes y después de someterlas a dos fases consecutivas de envejecimiento acelerado. También se han analizado obras originales con PVAc aplicado hace aproximadamente 30 años. El artículo presenta los resultados de las mediciones de color y pH en las muestras probeta antes y después de la primera fase de envejecimiento acelerado y, también, en las muestras envejecidas de forma natural durante 10 años.

Les pintures murals negres del monestir de Pedralbes: problemes de conservació-restauració causats per l’envelliment de l’acetat de polivinil

Aquesta tesi s’inicia com un treball de recerca i posteriorment passa a formar part delprojecte de recerca BHA2002-02411 fi nançat pel Ministerio de Ciencia y Tecnología, dins elPlan Nacional de I+D+I (2000-2003).La idea principal, però, s’origina a partir d’un encàrrec professional del Museu-Monestir dePedralbes i del Museu d’Història de la Ciutat l’any 2000.Les pintures murals negres (Figura 1) són una de les obres menys vistoses, de les que tenenmenys protagonisme, d’entre les que integren la col·lecció del Museu-Monestir de Pedralbes.No semblen, a priori, l’obra més interessant des del punt de vista d’un encàrrec professionalper a un conservador-restaurador. Però el poc que es coneix de les pintures negres i la sevararesa (es desconeixen altres pintures similars que s’hagin pogut conservar), així com lespatologies derivades del seu arrencament i traspàs l’any 1974, aconsellaven aprofundir en el seu estudi, que, mica en mica, va acabar resultant absorbent.