Screening test for antibiotics in medicinal plants (STAMP): using powdered plant materials instead of extracts

Coordenação de Aprefeiçoamento de Pessoal de Nível Superior (CAPES)

Insecticidal Effect of Extracts from Native Plants of Mato Grosso do Sul State on Sitophilus zeamais Mots (Coleoptera: Curculionidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Nitric oxide reduces oxidative damage induced by water stress in sunflower plants

Drought is one of the main environmental constraints that can reduce plant yield. Nitric oxide (NO) is a signal molecule involved in plant responses to several environmental stresses. The objective of this study was to investigate the cytoprotective effect of a single foliar application of 0, 1, 10 or 100 µM of the NO donor sodium nitroprusside (SNP) in sunflower plants under water stress. Water stressed plants treated with 1μM SNP showed an increase in the relative water content compared with 0 μM SNP. Drought reduced the shoot dry weight but SNP applications did not result in alleviation of drought effects. Neither drought nor water stress plus SNP applications altered the content of photosynthetic pigments. Stomatal conductance was reduced by drought and this reduction was accompanied by a significant reduction in intercellular CO2 concentration and photosynthesis. Treatment with SNP did not reverse the effect of drought on the gas exchange characteristics. Drought increased the level of malondialdehyde (MDA) and proline and reduced pirogalol peroxidase (PG-POD) activity, but did not affect the activity of superoxide dismutase (SOD). When the water stressed plants were treated with 10 μM SNP, the activity of PG-POD and the content of proline were increased and the level of MDA was decreased. The results show that the adverse effects of water stress on sunflower plants are dependent on the external NO concentration. The action of NO may be explained by its ability to increase the levels of antioxidant compounds and the activity of ROS-scavenging enzymes.

The length of the dry season may be associated with leaf scleromorphism in cerrado plants

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Chlorophyll a fluorescence in Annona emarginata (Schltdl.) H. Rainer plants subjected to water stress and after rehydration

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

In vitro antibacterial and chemical properties of essential oils including native plants from Brazil against pathogenic and resistant bacteria

The antimicrobials products from plants have increased in importance due to the therapeutic potential in the treatment of infectious diseases. Therefore, we aimed to examine the chemical characterisation (GC-MS) of essential oils (EO) from seven plants and measure antibacterial activities against bacterial strains isolated from clinical human specimens (methicillin-resistant Staphylococcus aureus (MRSA) and sensitive (MSSA), Escherichia coli, Pseudomonas aeruginosa, Salmonella Typhimurium) and foods (Salmonella Enteritidis). Assays were performed using the minimal inhibitory concentration (MIC and MIC90%) (mg/mL) by agar dilution and time kill curve methods (log CFU/mL) to aiming synergism between EO. EO chemical analysis showed a predominance of terpenes and its derivatives. The highest antibacterial activities were with Cinnamomun zeylanicum (0.25 mg/mL on almost bacteria tested) and Caryophyllus aronzaticus EO (2.40 mg/mL on Salmonella Enteritidis), and the lowest activity was with Eugenia uniflora (from 50.80 mg/mL against MSSA to 92.40 mg/mL against both Salmonella sources and P aeruginosa) EO. The time kill curve assays revealed the occurrence of bactericide synergism in combinations of C. aromaticus and C. zeylanicum with Rosmarinus. officinalis. Thus, the antibacterial activities of the EO were large and this can also be explained by complex chemical composition of the oils tested in this study and the synergistic effect of these EO, yet requires further investigation because these interactions between the various chemical compounds can increase or reduce (antagonism effect) the inhibitory effect of essential oils against bacterial strains.

Knowledge and use of wild edible plants in rural communities along Paraguay River, Pantanal, Brazil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Nitrogen metabolism in coffee plants in response to nitrogen supply by fertigation

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Effects of plant regulators on the growth and flowering of Saint Augustine grass plants

The objective of this work was to evaluate the effects of three plant growth inhibitors on the development and emission of floral rachis of Saint Augustine grass [Stenotaphrum secundatum (Walt.) Kuntzel] plants. The study was carried out in a 15 month old lawn with the experimental plots being distributed in accordance with a complete randomized block design with four replications. The treatments consisted of sole application of trinexapac-ethyl (113, 226, 452, 678, and 904 g ai ha(-1)), prohexadione-calcium (100 and 200 g ai ha(-1)), and bispyribac-sodium (40 and 60 g ai ha(-1)) plus a check treatment in which the plants were not submitted to any of the plant growth inhibitors. The effects of those products were evaluated in terms of visual signs of plant intoxication, plant height, emission and height of floral rachises, and chip total dry matter production. All the plant growth inhibitors resulted in visible injury to the plants but these intoxication signs practically disappeared 28 days after the application. Trinexapac-ethyl at the dose of 904 g ai ha(-1) reduced plant height by 59.7%, the emission of floral rachis by 96.4%, and the amount of chip dry matter production by 87.7%. Plant growth inhibitors may reduce the number of times of lawn plants cutting up to 119 days after their application with no harmful effects on the plants visual aspect.

Toothpick test: a methodology for the detection of RR soybean plants

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Life cycle of Raoiella indica (Acari: Tenuipalpidae) on ornamental plants, mostly Arecaceae

The red palm mite, Raoiella indica Hirst, has been primarily found associated with coconut and musaceous plants in the New World. However, it has also been recorded on several other palms, heliconiaceous and zingiberaceous species. This study was conducted to evaluate the suitability of different botanical families on which R. indica has been collected in the field and of arecaceous plants of the natural vegetation of the neotropics. In total, ten species of Arecaceae as well as Heliconia psittacorum [Heliconiaceae] and Alpinia purpurata [Zingiberacae] were evaluated, using coconut as a control. The study was carried out under controlled conditions (29 +/- A 0.5 A degrees C, 60 +/- A 10 % RH and photoperiod 12 h of light). Raoiella indica was able to complete immature development only on coconut, Adonidia merrillii, Ptychosperma macarthurii, H. psittacorum and A. purpurata. Duration of the immature phase (egg-adult) ranged between 21.5 days on coconut to 34.1 days on A. purpurata. Longevity was at least 50 % greater and oviposition at least 38 % higher on coconut than on other plants. Intrinsic rate of increase (r(m)) was higher on coconut (0.166) and A. merrillii (0.042), but negative on the other two plant species. Raoiella indica could not reach adulthood on any of the other ten arecaceous species considered in the study. The results suggested R. indica to be a threat to A. merrillii in addition to coconut, but not to other evaluated plants. However, complementary studies should be conducted to investigate whether the experimental procedures adopted in this study could not have prevented the mite from a better performance than it could have been under field conditions, especially in relation to Mauritia flexuosa, one of the dominant arecaceous plants in South America.

Foliar or soil applications of silicon alleviate water-deficit stress of potato plants

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)