A Strategy to Characterize the LISA-Pathfinder Cold Gas Thruster System

The cold gas micro-propulsion system that will be used during the LISA-Pathfinder mission will be one of the most important component used to ensure the "free-fall" of the enclosed test masses. In this paper we present a possible strategy to characterize the effective direction and amplitude gain of each of the 6 thrusters of this system.

A new insight about pharmaceutical dosage forms for benzathine penicillin G

In this work, a micellar system of benzathine penicillin G (BPG) in sodium deoxycholate (NaDC) was developed and evaluated physicochemically. The solubility profile of the drug in water and buffer solutions at various pH was determined, as well as its n-octanol/water partition coefficient. The Critical Micellar Concentration of NaDC and its ability to incorporate BPG were also assessed. The study was carried out at low and high ionic strength which was adjusted by the addition of sodium chloride. The results demonstrated the ability of the micellar system to incorporate BPG, as well as to increase its apparent solubility in water. The enhancement of the solubility of BPG by the presence of NaDC micelles could be analyzed quantitatively within the framework of the pseudo-phase model. Concentration analysis showed that the micellar system could attain up to 90% incorporation of BPG. The incorporated drug is expected to exhibit improved stability, since the antibiotic enclosed in the hydrophobic core of micelles is rather shielded from the aqueous external environment

Geomorphological constraining of tsunami (?) run-up in the Alcantarilha coastal lowland (Central Algarve, Portugal)

The Alcantarilha lowland, partly barred by a well developed barrier, including foredunes covering Pleistocene-Holocene beachrock and aeolianite, develops across the Alcantarilha infilled estuary, the beach-dune extending further SE until the Salgados lagoon. A topographic and coring survey revealed a peculiar feature at the leeward toe of the dune ridge close to the inlet area: a sandy fan with location, shape and morphology suggesting emplacement by single or multiple overwash of the barrier tip rather than tidal forcing. Its storm or tsunami origin and age are under investigation, and the only time-constrain available at present is that it should post-date ca. 6600 cal BP, the most recent in situ aeolianite (Moura et al., 2007) dated so far. METHODS, DATA SET AND RESULTS The fan boundaries are distinctive in aerial photos and satellite images: it is roughly ellipsoidal, ~200 m wide and ~300 m elongated paralleling the shoreline, rising ~ 0.9-1.2 m above the surrounding floodplain surface. Detailed topography shows that its short axis aligns with SW-NE elongated (though irregular) depressions in the dune crest, which link the beach with the fan. This could have favoured funnelling of, or erosion by, water overtopping the barrier but, in either case, the fan should correspond to extreme and abrupt event(s) of coastal flooding. 18 trenches and cores were performed in the exposed area of the fan and nearby flood plain to obtain samples and data on its sedimentology, lithostratigraphy and geometry. The fan consists of well sorted and rounded sand (Fig. 2). It thins away and wedges out landwards of the apex (located near Alc29T) where it is partly covered by dune sand. Its lower boundary is undulating and marked by textural contrast between sand (fan) and underlying mud (alluvial/lagoonal); an accumulation of marine-sourced perforated pebbles showing limited lateral continuity may pinpoint this boundary near the foredune (core Alc 25, ca. 80 m westward of profile in Fig.1); mud-balls were also observed immediately above this surface in cores and trenches. As the washover was probably emplaced in a barred lagoonal/estuarine floodplain setting, the fan’s northern outer belt is enclosed by low-energy sediments (not shown in Fig. 2).

Microbial nitrogen dynamics in organic and mineral soil horizons along a latitudinal transect in western Siberia

Soil N availability is constrained by the breakdown of N-containing polymers such as proteins to oligopeptides and amino acids that can be taken up by plants and microorganisms. Excess N is released from microbial cells as ammonium (N mineralization), which in turn can serve as substrate for nitrification. According to stoichiometric theory, N mineralization and nitrification are expected to increase in relation to protein depolymerization with decreasing N limitation, and thus from higher to lower latitudes and from topsoils to subsoils. To test these hypotheses, we compared gross rates of protein depolymerization, N mineralization and nitrification (determined using N-15 pool dilution assays) in organic topsoil, mineral topsoil, and mineral subsoil of seven ecosystems along a latitudinal transect in western Siberia, from tundra (67 degrees N) to steppe (54 degrees N). The investigated ecosystems differed strongly in N transformation rates, with highest protein depolymerization and N mineralization rates in middle and southern taiga. All N transformation rates decreased with soil depth following the decrease in organic matter content. Related to protein depolymerization, N mineralization and nitrification were significantly higher in mineral than in organic horizons, supporting a decrease in microbial N limitation with depth. In contrast, we did not find indications for a decrease in microbial N limitation from arctic to temperate ecosystems along the transect. Our findings thus challenge the perception of ubiquitous N limitation at high latitudes, but suggest a transition from N to C limitation of microorganisms with soil depth, even in high-latitude systems such as tundra and boreal forest.

Study of the development and evolution of seasonal thermocline and turbulent processes in the northern parts of Persian Gulf using numerical modeling

The Persian Gulf (PG) is a semi-enclosed shallow sea which is connected to open ocean through the Strait of Hormuz. Thermocline as a suddenly decrease of temperature in subsurface layer in water column leading to stratification happens in the PG seasonally. The forcing comprise tide, river inflow, solar radiation, evaporation, northwesterly wind and water exchange with the Oman Sea that influence on this process. In this research, analysis of the field data and a numerical (Princeton Ocean Model, POM) study on the summer thermocline development in the PG are presented. The Mt. Mitchell cruise 1992 salinity and temperature observations show that the thermocline is effectively removed due to strong wind mixing and lower solar radiation in winter but is gradually formed and developed during spring and summer; in fact as a result of an increase in vertical convection through the water in winter, vertical gradient of temperature is decreased and thermocline is effectively removed. Thermocline development that evolves from east to west is studied using numerical simulation and some existing observations. Results show that as the northwesterly wind in winter, at summer transition period, weakens the fresher inflow from Oman Sea, solar radiation increases in this time interval; such these factors have been caused the thermocline to be formed and developed from winter to summer even over the northwestern part of the PG. The model results show that for the more realistic monthly averaged wind experiments the thermocline develops as is indicated by summer observations. The formation of thermocline also seems to decrease the dissolved oxygen in water column due to lack of mixing as a result of induced stratification. Over most of PG the temperature difference between surface and subsurface increases exponentially from March until May. Similar variations for salinity differences are also predicted, although with smaller values than observed. Indeed thermocline development happens more rapidly in the Persian Gulf from spring to summer. Vertical difference of temperature increases to 9 centigrade degrees in some parts of the case study zone from surface to bottom in summer. Correlation coefficients of temperature and salinity between the model results and measurements have been obtained 0.85 and 0.8 respectively. The rate of thermcline development was found to be between 0.1 to 0.2 meter per day in the Persian Gulf during the 6 months from winter to early summer. Also it is resulted from the used model that turbulence kinetic energy increases in the northwestern part of the PG from winter to early summer that could be due to increase in internal waves activities and stability intensified through water column during this time.

Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia

Soil horizons below 30 cm depth contain about 60% of the organic carbon stored in soils. Although insight into the physical and chemical stabilization of soil organic matter (SUM) and into microbial community composition in these horizons is being gained, information on microbial functions of subsoil microbial communities and on associated microbially-mediated processes remains sparse. To identify possible controls on enzyme patterns, we correlated enzyme patterns with biotic and abiotic soil parameters, as well as with microbial community composition, estimated using phospholipid fatty acid profiles. Enzyme patterns (i.e. distance-matrixes calculated from these enzyme activities) were calculated from the activities of six extracellular enzymes (cellobiohydrolase, leucine-amino-peptidase, N-acetylglucosaminidase, chitotriosidase, phosphatase and phenoloxidase), which had been measured in soil samples from organic topsoil horizons, mineral topsoil horizons, and mineral subsoil horizons from seven ecosystems along a 1500 km latitudinal transect in Western Siberia. We found that hydrolytic enzyme activities decreased rapidly with depth, whereas oxidative enzyme activities in mineral horizons were as high as, or higher than in organic topsoil horizons. Enzyme patterns varied more strongly between ecosystems in mineral subsoil horizons than in organic topsoils. The enzyme patterns in topsoil horizons were correlated with SUM content (i.e., C and N content) and microbial community composition. In contrast, the enzyme patterns in mineral subsoil horizons were related to water content, soil pH and microbial community composition. The lack of correlation between enzyme patterns and SUM quantity in the mineral subsoils suggests that SOM chemistry, spatial separation or physical stabilization of SUM rather than SUM content might determine substrate availability for enzymatic breakdown. The correlation of microbial community composition and enzyme patterns in all horizons, suggests that microbial community composition shapes enzyme patterns and might act as a modifier for the usual dependency of decomposition rates on SUM content or C/N ratios. (C) 2015 The Authors. Published by Elsevier Ltd.

Backflow prevention certification workshop information and materials

The information enclosed in this booklet consists of memos, policy statements and other pertinent information to guide you in the establishment of a backflow prevention program.

Geomorphology and sedimentology of a modern isolated carbonate platform: The glorieuses archipelago, SW Indian Ocean

The study of modern carbonate systems is commonly helps in improving facies interpretation in fossil reefs and in providing analogues of sediment distribution depending on the specific platform configuration (i.e. rimmed shelves and isolated carbonate platforms). This paper deals with a geomorphological and sedimentological study of the Glorieuses Archipelago, an isolated carbonate platform located between the northern tip of Madagascar and Mayotte. The dataset consists of Digital Terrain Model, satellite imagery, and box-sediment samples. Analyses of grain-size and composition of carbonate grains are used to characterize the distribution and heterogeneity of sediment accumulated on the isolated platform. Main results show that the Glorieuses Archipelago is organized in distinctive morphological units, including a reef flat developed along the windward side, an apron, and a semi-enclosed (< 12 m water depth) to open lagoon (> 12 m and up to 15 m water depth). The lack of carbonate mud in sediments deposited on the archipelago can be explained by the direct connection between the lagoon and the open ocean. The main carbonate grains include Halimeda segments, coral fragments, large benthic foraminifers, red algae, and molluscs. According to the shape and the position of intertidal sandwaves, the current arrangement of moderately sorted fine to medium sands appears to be strongly influenced by tidal currents. The in-situ sediment production, accumulation and transport on the platform finally contribute to carbonate sand export to distinct deep marine areas depending on wind regimes and currents.

Exploring redox-driven self-assembly of mixed metal polyoxometalates

How can we control the experimental conditions towards the isolation of specific structures? Why do particular architectures form? These are some challenging questions that synthetic chemists try to answer, specifically within polyoxometalate (POM) chemistry, where there is still much unknown regarding the synthesis of novel molecular structures in a controlled and predictive manner. This work covers a wide range of POM chemistry, exploring the redox self-assembly of polyoxometalate clusters, using both “one-pot”, flow and hydrothermal conditions. For this purpose, different vanadium, molybdenum and tungsten reagents, heteroatoms, inorganic salts and reducing agents have been used. The template effect of lone-pair containing pyramidal heteroatoms has been investigated. Efforts to synthesize new POM clusters displaying pyramidal heteroanions (XO32-, where X= S, Se, Te, P) are reported. The reaction of molybdenum with vanadium in the presence of XO32- heteroatoms is explored, showing how via the cation and experimental control it is possible to direct the self-assembly process and to isolate isostructural compounds. A series of four isostructural (two new, namely {Mo11V7P} and {Mo11V7Te} and two already known, namely {Mo11V7Se} and {Mo11V7S} disordered egg-shaped Polyoxometalates have been reported. The compounds were characterized by X-ray structural analysis, TGA, UV-Vis, FT-IR, Elemental and Flame Atomic Absorption Spectroscopy (FAAS) analysis and Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). Cyclic Voltammetry measurements have been carried out in all four compounds showing the effect of the ionic density of the heteroatom on the potential. High-Resolution ESI-MS studies have revealed that the structures retain their integrity in solution. Efforts to synthesize new mixed-metal compounds led to isolation, structural, and electronic characterization of the theoretically predicted, but experimentally elusive δ-isomer of the Keggin polyoxometalate cluster anion, {H2W4V9O33(C6H13NO3)}, by the reaction of tungstate(VI) and vanadium(V) with triethanolammonium ions (TEAH), acting as a tripodal ligand grafted to the surface of the cluster. Control experiments (in the absence of the organic compound) have proven that the tripodal ligand plays crucial role on the formation of the isomer. The six vanadium metal centres, which consist the upper part of the cluster, are bonded to the “capping” TEA tripodal ligand. This metal-ligand bonding directs and stabilises the formation of the final product. The δ-Keggin species was characterized by single-crystal X-ray diffraction, FT-IR, UV-vis, NMR and ESI-MS spectrometry. Electronic structure and structure-stability correlations were evaluated by means of DFT calculations. The compounds exhibited photochromic properties by undergoing single-crystal-to-single-crystal (SC-SC) transformations and changing colour under light. Non-conventional synthetic approaches are also used for the synthesis of the POM clusters comparing the classical “one-pot” reaction conditions and exploring the synthetic parameters of the synthesis of POM compounds. Reactions under hydrothermal and flow conditions, where single crystals that depend on the solubility of the minerals under hot water and high pressure can be synthesized, resulted in the isolation of two isostructural compounds, namely, {Mo12V3Te5}. The compound isolated from a continuous processing method, crystallizes in a hexagonal crystal system, forming a 2D porous plane net, while the compound isolated using hard experimental conditions (high temperature and pressure) crystallizes in monoclinic system, resulting in a different packing configuration. Utilizing these alternative synthetic approaches, the most kinetically and thermodynamically compounds would possibly be isolated. These compounds were characterised by single-crystal X-ray diffraction, FT-IR and UV-vis spectroscopy. Finally, the redox-controlled driven oscillatory template exchange between phosphate (P) and vanadate (V) anions enclosed in an {M18O54(XO4)2} cluster is further investigated using UV-vis spectroscopy as a function of reaction time, showed that more than six complete oscillations interconverting the capsule species present in solution from {P2M18} to {V2M18} were possible, provided that a sufficient concentration of the TEA reducing agent was present in solution. In an effort to investigate the periodicity of the exchange of the phosphate and vanadate anions, time dependent Uv-vis measurements were performed for a period at a range of 170-550 hours. Different experimental conditions were also applied in order to investigate the role of the reducing agent, as well as the effect of other experimental variables on the oscillatory system.

Wysokociśnieniowe przemiany strukturalne kryształów węglowodanów

Wydział Chemii

‘YAMPIL INSPIRATIONS’: A STUDY OF THE DNIESTER CULTURAL CONTACT AREA AT THE FRONTIER OF PONTIC AND BALTIC DRAINAGE BASINS

The article presents the present state of research on the general issue of the dniester region of cultural contacts between communities settling the Baltic and Pontic drainage basins . Some five domains of research shall be brought to discussion in which it is possible to see fresh opportunities for archaeological study, on the basis of ‘Yampil studies’ on dniester-Podolia (forest-steppe) barrow-culture ceremonial centres from the latter half of the 4th millennium and first half of the 3rd millennium BC . This relates to the peoples of the Eneolithic and the Early Bronze age . in terms of topogenesis, embracing the Pontic-Tripolye, Yamnaya and Catacomb cultures, as well as Globular amphora and Corded ware in central prehistoric Europe .

Gestão do marketing de relacionamento: um estudo em empresas hoteleiras de Natal - RN

The present study it analyzes the Management of the Marketing of strategy Relationship as distinguishing for the host s companies of the city of Natal - RN. To carry through this analysis interviews with managers had been carried through, as well as the direct comment of processes, documents, actions and strategies developed for the hotels, with intention to know the level of perception and valuation of the relationship with customers, to verify resources and technologies used in the Management of the Relationship Marketing, identification, segmentation and differentiation of customers, personalization of products and services, and results of the emphasis in the relationship with customers for the host s companies. The research can be classified as exploratory - descriptive, and its universe is limited to the city of Natal, having enclosed hotels that have carried through tourist activity in 2005 and 2006. Still on the criteria of election of the sample, the study it investigated host s companies who if fit in the category superior luxury, or either, five stars, pertaining the national nets and international. How much to the treatment and analysis of the data the was made to leave of the theoretical support of the authors who work the thematic one and of the analysis of the interviews with managers, documents and processes observed for the researcher in the studied hotels. The research sample that the interviewed ones understand the importance to work the Management of the Marketing of Relationship in the host s companies me intention to get sustainable competitive advantage. One still evidenced that the searched hotels make use of strategies and instruments of Management of the Marketing of Relationship, however without an ample theoretical knowledge and yes only as base in the experience of the managers and spread processes already, generating one moment competitive advantage and not relationships of long duration

Envolvimento dos adolescentes com o álcool

Dissertação de Mestrado apresentada ao Instituto Superior de Psicologia Aplicada para obtenção de grau de Mestre na especialidade de Psicologia Educacional.

Rotation of Vibrio fischeri Flagella Produces Outer Membrane Vesicles That Induce Host Development

Using the squid-vibrio association, we aimed to characterize the mechanism through which Vibrio fischeri cells signal morphogenesis of the symbiotic light-emitting organ. The symbiont releases two cell envelope molecules, peptidoglycan (PG) and lipopolysaccharide (LPS) that, within 12 h of light organ colonization, act in synergy to trigger normal tissue development. Recent work has shown that outer membrane vesicles (OMVs) produced by V. fischeri are sufficient to induce PG-dependent morphogenesis; however, the mechanism(s) of OMV release by these bacteria has not been described. Like several genera of both beneficial and pathogenic bacteria, V. fischeri cells elaborate polar flagella that are enclosed by an extension of the outer membrane, whose function remains unclear. Here, we present evidence that along with the well-recognized phenomenon of blebbing from the cell's surface, rotation of this sheathed flagellum also results in the release of OMVs. In addition, we demonstrate that most of the development-inducing LPS is associated with these OMVs and that the presence of the outer membrane protein OmpU but not the LPS O antigen on these OMVs is important in triggering normal host development. These results also present insights into a possible new mechanism of LPS release by pathogens with sheathed flagella. IMPORTANCE Determining the function(s) of sheathed flagella in bacteria has been challenging, because no known mutation results only in the loss of this outer membrane-derived casing. Nevertheless, the presence of a sheathed flagellum in such host-associated genera as Vibrio, Helicobacter, and Brucella has led to several proposed functions, including physical protection of the flagella and masking of their immunogenic flagellins. Using the squid-vibrio light organ symbiosis, we demonstrate another role, that of V. fischeri cells require rotating flagella to induce apoptotic cell death within surface epithelium, which is a normal step in the organ's development. Further, we present evidence that this rotation releases apoptosis-triggering lipopolysaccharide in the form of outer membrane vesicles. Such release may also occur by pathogens but with different outcomes for the host.

Análise de selecção de áreas de caça por uma população de francelho (Faco naumanni) na planície de Évora

Em Portugal, apesar de haver registos históricos da reprodução desta espécie um pouco por todo o país, o francelho (Falco naumanni, Fleischer 1818) desapareceu de quase todo o território nacional estando actualmente restrito à região do Alentejo. Em 2005, 80% da população encontrava-se nas ZPE´s de Castro Verde e Vale do Guadiana (62% e 18% da população nacional, respectivamente (Catry et al. 2005). Ao longo dos últimos anos têm vindo a ser desenvolvidos trabalhos de investigação com o objectivo de conhecer as necessidades ecológicas da espécie nas diferentes colónias, de forma a tentar conciliar a prática de uma agricultura economicamente viável com a conservação de uma espécie que está dependente das práticas agrícolas tradicionais. Os conflitos entre conservação e alteração das práticas agrícolas levam a desafios na gestão deste tipo de habitats. A população de francelho da ZPE Évora, após um período de 12 anos de ausência da espécie como reprodutora na região, tem vindo a aumentar em número de casais reprodutores, desde 1995. A distribuição da espécie na ZPE de Évora, a sua evolução e os principais factores que afectam a sua reprodução foram por nós estudados no ano de 2007. Foi ainda analisada a disponibilidade de habitat de caça para o francelho na área que envolve a principal colónia de Évora de modo a poderem ser sugeridas medidas de gestão adequadas à sua conservação. Concluiu-se que a manutenção de áreas de caça e a conservação dos locais de nidificação existentes e a criação de novos locais passa inevitavelmente pela sensibilização e informação dos proprietários sobre as medidas a aplicar. O recurso a instrumentos de financiamento neste sentido será primordial; ABSTRACT: In Portugal, although there are historical records of the reproduction of this species all over the country, the lesser kestrel (Falco naumanni) disappeared from almost all the national territory being currently restricted to the Alentejo region. In 2005, 80% of the population was in Castro Verde and Vale do Guadiana SPA (62% and 18% of the national population, respectively) (Catry et al. 2005). Over the past few years have been developed research in order to meet the ecological needs of the species in the different colonies, trying to reconcile the practice of an economically viable agriculture and the conservation of a species that is dependent on traditional farming practices. The conflicts between conservation and changing farming practices lead to challenges in managing this type of habitat. The lesser kestrel population of the Évora SPA, after a 12 year absence of the species as breeding in the region, has being increasing in the number of breeding pairs, since 1995. The distribution of the species in the SPA of Évora, its evolution and the main factors affecting their reproduction were studied in 2007. It was also analyzed the availability of hunting habitat for the kestrel in the area surrounding the main colony of Évora so that appropriate management measures can be suggested to their conservation. It was concluded that maintaining hunting areas and conservation of existing nesting sites and the creation of new local inevitably passes through the awareness and information of the owners on the measures to be implemented. The use of financial instruments and the in this direction will be paramount.