Influence of Er,Cr:YSGG laser on CaFsub(2)-like products formation because of professional acidulated fluorides or to dentifrice application

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Energy transfer rates and population inversion of sup(4)Isub(11/2) excited state of Ersup(3+) investigated by means of numerical solutions of the rate equations system in Er:LiYFsub(4) crystal

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Relationship between surface topography and energy density distribution of Er, Cr:YSGG beam on irradiated dentin: an atomic force microscopy study

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Implication de TLE3 et KDM5A dans la régulation de la transcription des gènes cibles du récepteur des œstrogènes ERα

Dans le noyau cellulaire, l’ADN est compacté autour de petites protéines appelées histones formant ainsi le nucléosome, unité de base de la chromatine. Les nucléosomes contrôlent la liaison des facteurs de transcription à l’ADN et sont ainsi responsables de la régulation des processus cellulaires tels que la transcription. Afin de permettre l’expression des gènes, la chromatine est remodelée, c’est-à-dire que les nucléosomes sont repositionnés de manière à ce que la machinerie générale de la transcription puisse atteindre l’ADN afin de produire l’ARN messager. La moindre petite modification dans la fonction des facteurs de transcription ou des enzymes responsables du remodelage de la chromatine entraine des variations d’expression des gènes, et donc des maladies telles que les cancers. Le cancer du sein est le cancer le plus couramment développé chez les femmes. Cette maladie est principalement causée par l’activité du récepteur des œstrogènes ERα et de ses co-régulateurs ayant, pour la plupart, un rôle direct sur le remodelage de la chromatine. Afin de mieux comprendre le développement et la progression du cancer du sein, nous avons décidé d’étudier le rôle de deux co-régulateurs de ERα, TLE3 et KDM5A, impliqués dans le remodelage de la chromatine et dont la fonction dans le cancer du sein est indéterminée. Nous avons démontré que TLE3 est un partenaire d’interaction du facteur pionnier FoxA1, facteur nécessaire à la liaison de ERα sur l’ADN pour la transcription des gènes cibles de ce récepteur. L’interaction de TLE3 avec FoxA1 inhibe la liaison de ERα à l’ADN en absence d’œstrogènes, via le recrutement de HDAC2 qui déacétyle la chromatine, empêchant alors l’activation fortuite de la transcription en absence de signal. Quant à KDM5A, malgré sa réputation de répresseur de la transcription, dans le cancer du sein, cette déméthylase de H3K4me2/3 est un coactivateur de ERα, dû à son rôle direct sur l’expression du récepteur.

Radiative Behaviour of Gd2O3:Er/Yb nanoparticles under near infrared illumination

Upconverting nanoparticles have attracted much attention in science recently, specifically in view of medical and biological applications such as live imaging of cell temperatures or cancer treatment. The previously studied system of gadolinium oxide nanorods co-doped with erbium and ytterbium and decorated with different number densities of gold nanoparticles has been studied. So far, these particles have been proven as efficient nanothermometers in a temperature range from 300 up to 2000 K. In this work, a more detailed study on the morphological and radiative behaviour of these particles has been conducted. It was found that the laser power threshold for the onset of the black body radiation decreases strongly with the increase in the gold concentration. The temperature of the onset itself seems to remain approximately constant. The heating efficiency was determined to increase significantly with the gold concentration. The morphological study revealed that the temperature at the black body radiation threshold was not enough to induce any significant transformation in neither the nanorods nor the gold nanoparticles, as was expected from comparison with literature. However, significant changes in radiative properties and the morphology were detected for powders that underwent strong laser heating until the emission of brightly visible black body radiation.

TFG Promotes Organization of Transitional ER and Efficient Collagen Secretion

Collagen is the most abundant protein in the animal kingdom. It is of fundamental importance during development for cell differentiation and tissue morphogenesis as well as in pathological processes such as fibrosis and cancer cell migration. However, our understanding of the mechanisms of procollagen secretion remains limited. Here, we show that TFG organizes transitional ER (tER) and ER exit sites (ERESs) into larger structures. Depletion of TFG results in dispersion of tER elements that remain associated with individual ER-Golgi intermediate compartments (ERGICs) as largely functional ERESs. We show that TFG is not required for the transport and packaging of small soluble cargoes but is necessary for the export of procollagen from the ER. Our work therefore suggests a key relationship between the structure and function of ERESs and a central role for TFG in optimizing COPII assembly for procollagen export.

Multimodal iron oxide (Fe₃O₄)-saturated lactoferrin nanocapsules as nanotheranostics for real-time imaging and breast cancer therapy of claudin-low, triple-negative (ER-/PR-/HER2-)

AIM: To unravel the multimodal nanotheranostic ability of Fe3O4-saturated bovine lactoferrin nanocapsules (FebLf NCs) in claudin-low, triple-negative breast cancer model. MATERIALS & METHODS: Xenograft study was performed to examine biocompatibility, antitumor efficacy and multimodal nanotheranostic action in combination with near-infrared live mice imaging. RESULTS: FebLf NCs exhibited a size range of 80 nm ± 5 nm with observed superparamagnetism. FebLf NCs successfully internalized into breast cancer cells through receptor-mediated endocytosis and induced apoptosis through the downregulation of inhibitor of apoptosis survivin and livin proteins. Investigations revealed a remarkable biocompatibility, anticancer efficacy of the FebLf NCs. Near-infrared imaging observations confirmed selective localization of multimodal FebLf NCs at the tumor site and lead to time-dependent reduction of tumor growth. CONCLUSION: FebLf NCs can be safe, biocompatible nanotheranostic approach for real-time imaging and monitoring the effect of drugs in real time and have potentials in future clinical trials.