Histological and global gene expression analysis of the ‘lactating’ pigeon crop

Background: Both male and female pigeons have the ability to produce a nutrient solution in their crop for the nourishment of their young. The production of the nutrient solution has been likened to lactation in mammals, and hence the product has been called pigeon ‘milk’. It has been shown that pigeon ‘milk’ is essential for growth and development of the pigeon squab, and without it they fail to thrive. Studies have investigated the nutritional value of pigeon ‘milk’ but very little else is known about what it is or how it is produced. This study aimed to gain insight into the process by studying gene expression in the ‘lactating’ crop.
Results: Macroscopic comparison of ‘lactating’ and non-’lactating’ crop reveals that the ‘lactating’ crop is enlarged and thickened with two very obvious lateral lobes that contain discrete rice-shaped pellets of pigeon ‘milk’. This was characterised histologically by an increase in the number and depth of rete pegs extending from the basal layer of the epithelium to the lamina propria, and extensive proliferation and folding of the germinal layer into the superficial epithelium. A global gene expression profile comparison between ‘lactating’ crop and non-’lactating’ crop showed that 542 genes are up-regulated in the ‘lactating’ crop, and 639 genes are down-regulated. Pathway analysis revealed that genes up-regulated in ‘lactating’ crop were involved in the proliferation of melanocytes, extracellular matrix-receptor interaction, the adherens junction and the wingless (wnt) signalling pathway. Gene ontology analysis showed that antioxidant response and microtubule transport were enriched in ‘lactating’ crop.
Conclusions: There is a hyperplastic response in the pigeon crop epithelium during ‘lactation’ that leads to localised cellular stress and expression of antioxidant protein-encoding genes. The differentiated, cornified cells that form the pigeon ‘milk’ are of keratinocyte lineage and contain triglycerides that are likely endocytosed as very low density lipoprotein (VLDL) and repackaged as triglyceride in vesicles that are transported intracellularly by microtubules. This mechanism is an interesting example of the evolution of a system with analogies to mammalian lactation, as pigeon ‘milk’ fulfils a similar function to mammalian milk, but is produced by a different mechanism.

Exploring the global and local networks of national information system integration initiatives : a case study of national cultural collection integration from an actor-network perspective

Australian Museums Online (AMOL) was the earliest attempt to make Australia’s distributed cultural collections accessible from a single online resource. Despite early successes, significant achievements and the considerable value it offered certain groups, the project ran into operational difficulties and was eventually discontinued. By using Actor-Network Theory and analysing the global and local actor-networks, it is revealed that although the project originated from large, state museums, buy-in was restricted to individuals, rather than institutions and the most significant value was for smaller, regional institutions. Furthermore, although the global networks that governed the project could translate their visions through the local production networks, because the network’s underlying weaknesses were never addressed, over time this destablised the global networks. This case study offers advice for projects attempting to consolidate data sources from disparate sources, and highlights the importance of individual actors in championing the project.

PGSW-OS: a novel approach for resource management in a semantic web operating system based on a P2P grid architecture

A web operating system is an operating system that users can access from any hardware at any location. A peer-to-peer (P2P) grid uses P2P communication for resource management and communication between nodes in a grid and manages resources locally in each cluster, and this provides a proper architecture for a web operating system. Use of semantic technology in web operating systems is an emerging field that improves the management and discovery of resources and services. In this paper, we propose PGSW-OS (P2P grid semantic Web OS), a model based on a P2P grid architecture and semantic technology to improve resource management in a web operating system through resource discovery with the aid of semantic features. Our approach integrates distributed hash tables (DHTs) and semantic overlay networks to enable semantic-based resource management by advertising resources in the DHT based upon their annotations to enable semantic-based resource matchmaking. Our model includes ontologies and virtual organizations. Our technique decreases the computational complexity of searching in a web operating system environment. We perform a simulation study using the Gridsim simulator, and our experiments show that our model provides enhanced utilization of resources, better search expressiveness, scalability, and precision. © 2014 Springer Science+Business Media New York.

Experimental and simulation study of the impact of increased photovoltaic integration with the grid

The abundance, availability, and climate-friendly characteristics of solar photovoltaic (PV) energy encourage nations around the globe to adopt it to assist in overcoming global warming as well as build a sustainable society for the future. The intermittent nature of solar energy generation and the associated power electronic inverters with connected consumer loads creates a number of potential challenges in integrating large-scale PV into the grid that affects power quality of the distribution networks. This paper investigates the impacts of varying PV integration into the grid through experimental and simulation studies. Initially, several experiments were conducted with varying PV penetration and load conditions using the Renewable Energy Integration Facility at CSIRO, Newcastle, Australia. Later, a simulation model was developed that mimics the experimental facility used at CSIRO to investigate the adverse impacts on integrating large-scale PV into the grid using the power system simulation software PSS Sincal. Experimental and simulation analyses clearly indicate that integration of PV into the grid causes power quality issues such as voltage instability, harmonic injection, and low power factor into the networks and the level of these impacts increases with the increase of PV penetration. © 2014 AIP Publishing LLC.

A dynamic time warped clustering technique for discrete event simulation-based system analysis

Abstract This paper introduces a novel approach for discrete event simulation output analysis. The approach combines dynamic time warping and clustering to enable the identification of system behaviours contributing to overall system performance, by linking the clustering cases to specific causal events within the system. Simulation model event logs have been analysed to group entity flows based on the path taken and travel time through the system. The proposed approach is investigated for a discrete event simulation of an international airport baggage handling system. Results show that the method is able to automatically identify key factors that influence the overall dwell time of system entities, such as bags that fail primary screening. The novel analysis methodology provides insight into system performance, beyond that achievable through traditional analysis techniques. This technique also has potential application to agent-based modelling paradigms and also business event logs traditionally studied using process mining techniques.