Co-oscillation of a fjord basin’s circulation with decade-long oceanographic, hydrologic, and climatic regimes: Puget Sound 1916-'87 [abstract]

EXTRACT (SEE PDF FOR FULL ABSTRACT): Oceanographic, hydrologic, and climatic data collected during 1916-'87 in Puget Sound's Main Basin (~200 m x 5 km x 100 km) and approaches oscillate at low frequency between two regimes (I, II). The oscillation accounts for a large fraction of the interannual variability (41-75%) and the zero crossings between regimes span approximately a decade. ... The transition between regimes is accompanied by substantial changes in the horizontal pressure and density fields between the Pacific coast and the mixing zones leading to the Basin, as well as within the Basin itself.

Co-occurrence of bycatch and target species in the groundfish demersal trawl fishery of the U.S. west coast; with special consideration of rebuilding stocks

Bycatch and resultant discard mortality are issues of global concern. The groundfish demersal trawl fishery on the west coast of the United States is a multispecies fishery with significant catch of target and nontarget species. These catches are of particular concern in regard to species that have previously been declared overfished and are currently rebuilding biomass back to target levels. To understand these interactions better, we used data from the West Coast Groundfish Observer Program in a series of cluster analyses to evaluate 3 questions: 1) Are there identifiable associations between species caught in the bottom trawl fishery; 2) Do species that are undergoing population rebuilding toward target biomass levels (“rebuilding species”) cluster with targeted species in a consistent way; 3) Are the relationships between rebuilding bycatch species and target species more resolved at particular spatial scales or are relationships spatially consistent across the whole data set? Two strong species clusters emerged—a deepwater slope cluster and a shelf cluster—neither of which included rebuilding species. The likelihood of encountering rebuilding rockfish species is relatively low. To evaluate whether weak clustering of rebuilding rockfish was attributable to their low rate of occurrence, we specified null models of species occurrence. Results indicated that the ability to predict occurrence of rebuilding rockfish when target species were caught was low. Cluster analyses performed at a variety of spatial scales indicated that the most reliable clustering of rebuilding species was at the spatial scale of individual fishing ports. This finding underscores the value of spatially resolved data for fishery management.

Distribution and co-occurrence of rockfishes (family: Sebastidae) over trawlable shelf and slope habitats of California and southern Oregon

The rockfishes of the sebastid genus Sebastes are a very important fishery resource off the coasts of California and southern Oregon. How-ever, many of the 54 managed stocks of west coast rockfish have recently reached historically low population levels, leading fishery managers to re-examine current management practices. Management of rockfish stocks as multispecies aggregates, as opposed to independent stocks within the ground-fish fishery, can be more desirable when nontargeted bycatch, discard, and management complexity are considered. Rockfish assemblage structure and species co-occurrences were determined by using data from the Alaska Fisheries Science Center triennial continental shelf bottom trawl survey. The weight of rockfish species in trawl catches was expressed as a catch-per-unit-of-effort (CPUE) statistic, from which species spatial distributions, overlaps, diversity, and richness were analyzed. Multidimensional scaling of transformed CPUE data was employed in indirect gradient and multivariate partitioning analyses to quantify assemblage relationships. Results indicated that rockfish distributions closely match the bathymetry of coastal waters. Indirect gradient analysis suggested that depth and latitude are the principal factors in structuring the spatial distributions of rockfish on trawlable habitat. In addition, four assemblages were identified through the joint evaluation of species’ distributions and multivariate partitioning analyses: 1) deep-water slope; 2) northern shelf; 3) southern shelf; and 4) nearshore. The slope, shelf, and near-shore groups are found in depth ranges of 200–500 m, 100–250 m, and 50–150 m, respectively. The division of northern and southern shelf assemblages occurs over a broad area between Cape Mendocino and Monterey Canyon. The results of this analysis are likely to have direct application in the management of rockfish stocks off the coasts of southern Oregon and California.

Co-expression of CD173 (H2) and CD174 (Lewis Y) with CD44 suggests that fucosylated histo-blood group antigens are markers of breast cancer-initiating cells

Histo-blood group antigens CD173 (H2) and CD174 (Lewis Y) are known to be developmentally regulated carbohydrate antigens which are expressed to a varying degree on many human carcinomas. We hypothesized that they might represent markers of cancer-initiating cells (or cancer stem cells, CSC). In order to test this hypothesis, we examined the co-expression of CD173 and CD174 with stem cell markers CD44 and CD133 by flow cytometry analysis, immunocytochemistry, and immunohistochemistry on cell lines and tissue sections from breast cancer. In three breast cancer cell lines, the percentage of CD173(+)/CD44(+) cells ranged from 17% to > 60% and of CD174(+)/CD44(+) from 21% to 57%. In breast cancer tissue sections from 15 patients, up to 50% of tumor cells simultaneously expressed CD173, CD174, and CD44 antigens. Co-expression of CD173 and CD174 with CD133 was also observed, but to a lesser percentage. Co-immunoprecipitation and sandwich ELISA experiments on breast cancer cell lines suggested that CD173 and CD174 are carried on the CD44 molecule. The results show that in these tissues CD173 (H2) and CD174 (LeY) are associated with CD44 expression, suggesting that these carbohydrate antigens are markers of cancer-initiating cells or of early progenitors of breast carcinomas.