Polymorphisms in the C-type lectin genes cluster in chromosome 19 and predisposition to severe acute respiratory syndrome coronavirus (SARS-CoV) infection

Background: Polymorphisms of CLEC4M have been associated with predisposition for infection by the severe acute respiratory syndrome coronavirus (SARS-CoV). DC-SIGNR, a C-type lectin encoded by CLEC4M, is a receptor for the virus. A variable number tandem

17种植物中蛋白质提取物的抗HIV-1活性

以化合物对ＨＩＶ１诱导Ｃ８１６６细胞形成合胞体的抑制实验和化合物对ＨＩＶ１感染细胞的保护实验作为初筛方法，筛选了来源于７科１７种植物的４７个样品，其中８个样品经测定是核糖体失活蛋白（ＲＩＰｓ），其余为粗提蛋白。进而测定了初筛有抗ＨＩＶ活性的化合物对共培养、急性和慢性感染的ＨＩＶ１ｐ２４抗原表达水平的影响，用间接荧光染色检测这些化合物对ＨＩＶ抗原阳性细胞率的影响，以确证其抗ＨＩＶ活性。天花粉蛋白（ＴＣＳ）、南方栝楼蛋白峰Ⅴ、南方栝楼蛋白峰Ⅵ均显著地抑制合胞体的形成；巴Ⅱ、丝瓜子蛋白、油瓜根蛋白、木盍藤子蛋白有一定的抑制作用。老鼠拖瓜蛋白、大叶木鳖子根蛋白、西双版纳根Ⅱ等粗提蛋白有显著的抑制作用。ＲＩＰｓ不能保护ＨＩＶ１感染细胞的死亡。ＴＣＳ显著地抑制了ＨＩＶ１急性感染中ｐ２４抗原的表达，减少了ＨＩＶ抗原阳性细胞数，但它们均不影响共培养细胞融合、ＨＩＶ１慢性感染中ｐ２４抗原表达水平。结果表明，除ＴＣＳ等已知ＲＩＰｓ外，还有一些新的ＲＩＰｓ具有不同程度的抗ＨＩＶ活性。

Import substitution of combination wire rope. Part 2. Production and standardisation of 17 mm dia combination wire rope.

The prototype combination wire rope (Cift-CWR 1) developed for deep sea trawling was further studied for improvement, optimisation of efficiency and standardization. A series of improved prototype combination wire ropes (Cift-CWR 2 to 6) were twisted and evaluated their mechanical properties and reported in this paper with recommendations for a standard 17mm dia combination wire rope of 6S (7C+8+1 Scr) + 6 Crs(6+1+1 Crc) construction.

Quality assessment of traditional, rotary and solar tunnel dried small indigenous fish species products

Studies on the quality assessments of three traditional, rotary and solar tunnel dried SIS products were conducted. Organoleptic quality of traditional dried SIS products available in the markets was poor compared to those produced in rotary and solar tunnel dryer. Reconstitution of samples were in the range of 54.26% to 75.24%, 69.37% to 83.73% and 55.08% to 80.24% when soaked at 80°C for traditional, rotary and solar tunnel dried products, respectively. The percentage of reconstitution increased with the increase of soaking time and the uptake of water was maximum after 60 min of soaking. The moisture contents of traditional, rotary and solar tunnel dried products were in the range of 26.02% to 27.33%, 16.23% to 22.84% and 13.71% to 19.30%, respectively. The protein contents were in the range of 60.78% to 72.59%, 62.17% to 76.27% and 61.11% to 76.00%, respectively; lipid contents were in the range of 12.26% to 22.60%, 14.00% to 24.71% and 13.92% to 22.39%, respectively and ash contents in the range of 15.11% to 16.59%, 8.32% to 13.51% and 8.71% to 16.45%, respectively on dry matter basis. The TVB-N content of rotary and solar tunnel dried products was low compared to traditional one ranging from 10.64 to 17.52 mg/100g and 14.34 to 15.68 mg/100g, respectively whereas the TVB-N content of traditional samples was in the range of 15.46 to 20.36 mg/100g. The bacterial load of traditional, rotary and solar tunnel dried products were in the range of 1.43x10 super(8) CFU/g to 2.89 x10 super(80 CFU/g, 1.91x10 super(8) CFU/g to 2.84x10 super(8) CFU/g and 1.95x10 super(8) CFU/g to 2.59x10 super(8) CFU/g, respectively. The results of the study indicated that dried fish products from rotary dryer and solar tunnel dryer were found to be of better quality in nutritional and food quality aspects than those of traditional dried products.

Species diversification in coastal aquaculture: production potentials of shrimp (Penaeus monodon) with mono and mixed sex tilapia

An experiment of 120 days of culture was conducted in brackishwater earthen ponds having an area of 0.2ha each. The hatchery produced shrimp (Penaeus monodon) post larvae were stocked in the 40m² fine meshed nylon net nursery enclosures were fed with commercial pellet feed. After two weeks of nursing, juveniles were allowed to spread in cultural pond by opening the fence. Fingerlings of three different strain of tilapia were stocked as shrimp and Strain-1 all male (monosex) (T1), shrimp and Strain-2 all male (T2), shrimp and Strain-3 mixed sex population (T3) @ 20.000/ha and 10.000/ha, respectively and shrimp only (monoculture) (T4) @ 20.000/ha. The shrimp and fish were fed with farm made feed consisting of a mixture of fishmeal 29%, MOC 15%, rice bran 30%, soybean meal 16%, wheat flour 9% and vitamin premix 0.1%. The average final weight of shrimp was 24.9±1.13g, 23.41±3.26g and 26.67±1.89g that stocked with tilapia in treatments T1, T2, and T3 respectively. The final average weight of shrimp in monoculture (T4) was 27.41±0.76g, apparently higher but insignificant in treatments. The survival of shrimp was 42.17%, 32.38%, 39.45% and 61.98% in treatments T1 T2, T3 and T4 respectively. The production of shrimp in concurrent culture was 193.67, 154.26 and 210.41kg/ha in T1, T2 and T3, respectively, while in monoculture (T4) was 339.77 kg/ha. The growth and survival of tilapia among the treatments was insignificant. The growth of monosex tilapia ranged 225.29 and 291.31g and survival 62.77 and 72.20% in T1 and T2, respectively, in mixed sex was 193.0g and 83.20% (T3). The production of tilapia monosex strains was 1676.69kg/ha (Strain-2 all male) and 1668.98 kg/ha (Strain-1 all male) while that of Strain-3 mixed sex population was 1622.92 kg/ha.

Evaluation of heavy metals along the Mediterranean coastal waters of the Nile Delta region, Egypt

This work focuses on four marine sites in the Mediterranean Sea around the Nile Delta, Egypt. Surface water samples were collected seasonally during 2003. The concentrations of some heavy metals in dissolved form (Fe, Mn, Zn, Cu, Ni and Pb) are evaluated. The levels of heavy metals in the coastal waters were 11.92-30.4512µglˉ¹ for Fe; 5.79-17.36 µglˉ¹ for Zn; 0.30-0.83µglˉ¹ for Cu; 0.51-2.90µglˉ¹ for Ni and 0.53-10.31µglˉ¹ for Pb. These are compared, with sites in the estuaries and outlets of the Nile Delta. Fe (19.72-60.33µglˉ¹); Mn (12.63-35.60µglˉ¹); Zn (2.67-22.00); Cu (0.56-1.67µglˉ¹); Ni (1.43-3.73µglˉ¹); Pb (1.72-59.7µglˉ¹). The results showed a remarkable decrease in the concentrations of different heavy metals with increased salinity. Comparing the present data with the minimal risk concentration reported by WQC, the distribution of heavy metals was significantly lower in coastal sea water of the Mediterranean Sea off Egypt. The study indicated also that the average contents of Ni and Pb are slightly high in the area of water exchange than those reported by WQC.

The study of biological characteristic and population dynamic of Caspian vimba (Vimba vimba persa), in coastal waters of Caspian Sea (Mazandaran Province)

This study was conducted to determine reproduction characteristics, diet regime, age structure and population dynamics parameters of the vimba vimba persa (Pallas, 1811) in Mazandaran waters of the Caspian Sea, from October 2008 to September 2009. A total of 994 specimens were monthly collected by beach seine and cast net from six fish landings of Ramsar, Tonekabon, Chaloos, Mahmood Abad, Sari and Behshahr. Biometric characters were measured for each specimen at the laboratory. Scales were used for age determination. Sex determination and fecundity were determined. Population dynamic parameters as well as stock assessment including cohort analysis were estimated using FISAT software. The finding showed that the mean of fork length and body weight of the Caspian Vimba were 168.4±2.6 mm and 71.94±32.24 g respectively. Strong correlation was found between these two variables (a= 0.012; b = 3.047; r2 = 0.955). 92 specimens were studied from the fecundity point of view. This species was found to have more abundance in spring (esp. Apr-May). The samples composed of 397(42.6%) male, 537(57.4%) female; Overall sex ratio (M: F =1: 1.35) was significantly different from the expected 1:1 ratio (p ≤0.05). The advanced stages of maturity (4th & 5th) were found in April and May. The highest Gonadosomatic Index in female was in May and the lowest one was in July. This fish is therefore a spring spawner. The maximum absolute and relative fecundities were 34640 and 260.9, respectively; the minimum absolute and relative fecundities were 5400 and 94.5 respectively. The averages of absolute and relative fecundities were 17198±7710 and 171.85±48.8, respectively. Coefficient vacuity index was 59.2% which indicates that this fish is mesophagous. Among of living creature consumes by Caspian Vimba mollusks, 76 arthropods, worms, plants, detritus and fishes were found 32.9% , 26.7% , 13.4% , 17% , 4.4% and 1.6% respectively. The infinite fork lengths were 261 mm for females, 25mm for males and 261 mm for both sexes respectively. For population growth and mortality parameters; K ( 0.28 per year for both sexes, 0.3 per year for males, 0.33 per year for females); t0 ( -0.65 year for both sexes, -0.23 year in females, -0.51 year in males ); Φ' ( 2.28 ); Z ( 0.98 per year ); M ( 0.59 per year); F ( 0.39 per year) and Exploitation coefficient was 0.4. The analysis showed that total biomass and MSY were 1336 and 528.8 tonnes respectively.

Effect of two green algae species (Chlorella sp. & Scenedesmus obliquus) enriched with B group vitamins on food chain of Rutilus frisii kutum (Kamensky, 1901) fry

The Effect of two freshwater green algae species Chlorella sp. & Scenedesmus obliquus enriched (from the beginning of culture and after 96 hours) with different dosages of B group vitamins (0, 0.5, 1, and 2 ml of enriching solution per each liter of algae medium) on fecundity of Daphnia magna and growth of Rutilus frisii kutum fry were investigated in a research from spring, 2008 to autumn, 2009. First, each of the green algae species were cultured purely and massively in the Zander (Z-8+N) medium and then the nutritional value (the amount of protein, lipid, and carbohydrate) of enriched algae were meausered. In this study, enriching of Chlorella sp. & S. obliquus with a suitable mix of B group vitamins significantly improved their nutritive value. So the highest amount of nutritional value of Chlorella sp. was obtained because of enriching with dosage 0.5 ml.l-1 (366.654Kcal) and for Scenedesmus obliquus with dosage of 1 ml.l-1 (376.95Kcal). The acquired amount from control group showed an increase of respectively 42% and 11%. According to the results, increased dosages of enriching solution caused Daphnia fecundity to increase (at both stages : enrichment from the beginning of culture and after 96 hours). So the highest average of D. magna reproduction rate was obtained through being fed with Chlorella sp. and S. obliquus enriched with dosage of 2 ml enriching solution per liter of algae medium. The average fecundity of D. magna fed with Chlorella sp. enriched with dosage of 2 ml.l-1 enriching solution from the beginning of culture and after 96 hours was obtained respectively 2.128 ± 0.375 and 2.1 ± 0.69 and the average fecundity of D. magna fed with S. obliquus enriched with dosage of 2 ml enriching solution from the beginning of culture and after 96 hours was obtained respectively 2.128 ± 0.375 and 2.1 ± 0.69 which showed respectively an increase of 61 ٪, 91٪, 77 ٪, and 83٪ in proportion to the acquired amount from control group. When enriching solution was added to either algae culture medium from the beginning of culture, showed statistically significant differences (P<0.05) between dosages of 0 and 2 ml.l-1, 1 and 2 ml.l-1, and 0.5 and 2 ml enriching solution per each liter of Chlorella sp. culture medium and between dosages of 0 and 1 ml.l-1, and 0 and 2 ml enriching solution per each liter of S. obliquus culture medium. The highest average of body weight gain percentage and specific growth rate of kutum fry was obtained respectively 21.19%, 26.63%, 1.92, and 2.34 from the beginning of culture and after 96 hours with dosage of 1 ml B group vitamins per each liter of Chlorella sp. culture medium, which showed respectively an increase of 50%, 70%, 46%, and 62% in proportion to the acquired amount from control group. In the cases which Chlorella sp. were grown in the medium containing vitamin, from point of view of the average percentage of weight and specific growth rate of kutum fry significant differences were observed on the basis of the result of One-way ANOVA between dosages of 0 and 1, 1 and 2 , 0.5 and 1 ml B group vitamins per each liter. The highest average of body weight gain percentage and specific growth rate of kutum fry was obtained respectively 32.02%, 29.42%, 2.78, and 2.34 from the beginning of culture and after 96 hours with dosage of 2 ml B group vitamins per each liter of S. obliquus culture medium, which showed respectively an increase of 32%, 19%, 28%, and 17% in proportion to the acquired amount from control group. In the cases which S. obliquus were grown in the medium containing vitamin, from point of view of the average percentage of weight and specific growth rate of kutum fry significant differences were observed on the basis of the result of One-way ANOVA between dosages of 0 and 1, 0 and 2. According to the results of the present research we can say that considerable enhancement in the quality of the food of D. magna can be made by manipulation of the nutritional value of fresh water unicellular green algae with suitable mixture of B group vitamins, so that both the fecundity of D. magna will increase and the nutritional requirements of the kutum fry will be filled in this way.

Report on BOBLME Indian mackerel genetic data analysis including final workshop, Phuket, Thailand, 17-19 February 2015

This workshop followed on from two previous workshops held in Colombo, Sri Lanka, 2012 and Kochi, India in 2013. The 14 microsattellite markers had previously been developed for Indian Mackerel (Rastrelliger kanagurta) were used on 31 tissue collections from all eight countries were genotyped in India.

Molecular phylogeny and taxonomy of wood mice (genus Apodemus Kaup, 1829) based on complete mtDNA cytochrome b sequences, with emphasis on Chinese species

Phylogenetic relationships among 15 species of wood mice (genus Apodemus) were reconstructed to explore some long-standing taxonomic problems. The results provided support for the monophyly of the genus Apodemus, but could not reject the hypothesis of paraphyly for this genus. Our data divided the 15 species into four major groups: (1) the Sylvaemus group (A. sylvaticus, A. flavicollis, A. alpicola, and A. uralensis), (2) the Apodemus group (A. peninsulae, A. chevreri, A. agrarius, A. speciosus, A. draco, A. ilex, A. semotus, A. latronum, and A. mystacinus), (3) A. argenteus, and (4) A. gurkha. Our results also suggested that orestes should be a valid subspecies of A. draco rather than an independent species; in contrast, A. ilex from Yunnan may be regarded as a separate species rather than a synonym of orestes or draco. The species level status of A. latronum, tscherga as synonyms of A. uralensis, and A. chevrieri as a valid species and the closest sibling species of A. agrarius were further corroborated by our data. Applying a molecular clock with the divergences of Mus and Rattus set at 12 million years ago (Mya) as a calibration point, it was estimated that five old lineages (A. mystacinus and four major groups above) diverged in the late Miocene (7.82-12.74 Mya). Then the Apodemus group (excluding A. mystacinus) split into two subgroups: agrarius and draco, at about 7.17-9.95 Mya. Four species of the Sylvaemus group were estimated to diverge at about 2.92-5.21 Mya. The Hengduan Mountains Region was hypothesized to have played important roles in Apodemus evolutionary histories since the Pleistocene. (C) 2004 Elsevier Inc. All rights reserved.

Meeting on BOBLME engagement in the International Indian Ocean Expedition 50th anniversary initiative (IIOE-2), Bangkok, Thailand, 17-18 March, 2015

Objectives included; a contribution to understanding large-scale processes affecting the Bay of Bengal Large Marine Ecosystem Project (BOBLME) and it's living resources; and to align with International Indian Ocean Expedition (IIOE-2) (2015-2020) which will extensively explore and study the Indian Ocean to improve understanding of the ocean and coupled climate processes.

Fish exploitation and changes in the fish community of Lake George, Uganda

Unlike Lake Victoria, the fisheries of Lake George have undergone gradual changes in the size and proportion of the major commercial fish species, the Nile tilapia (Oreochromis niloticus: cichlidae) in the last 40 years (1950-1989). The size decreased from an average weight of 900g in 1950 to 430g in 1989 while percentage contribution in commercial catches during the same period declined from 92% to 36%. The over all annual commercial catches though showed a steady increase from the period 1950 when the fishery was opened to intensive and controlled exploitation, consistently high catches were observed in the 1960s and 1970s followed by a general decline in the early 1980s to amore or less stable fishery in the late 1980s. These changes are attributed to increased fishing pressure especially on the nil tilapia and to increased use of smaller gill net mesh sizes lower than the recommended 127mm mesh. The changes in gill net mesh have brought O. leucostictus, acichlid, into commercial catches confirming that the 88.9mm mesh size nets are used by the commercial fishermen to harvest smaller fish species. The commercial catches are presently dominated by the piscivorous fishes,(over 60%) whose contribution was less than 10% during initial exploitation of the virgin fishery in 1950.The piscivorous fish are mainly caught using hooks and lines. The entire fishery is believed to be exploited close to the maximum. The above trends serve to show the impact of exploitation on fish species diversity. Quantitive and qualitative changes of the major fish species on lake George are due to exploitation pressure unlike Lake Victoria where it is a combination of both exploitations and impact of fish introductions. There has been no fish introduction in Lake George.

A decline in fish catches and the size of Oreochromis niloticus in Lake Wamala (Uganda) following human exploitation

The fishery of Lake Wamala has declined since the lake was stocked in 1956 and opened to fishing during the 1960s. Surveys were conducted on the lake during 1975/78 and 1988/92 to investigate the causes of declining fish catches. The lake produced an average of 4000 - 6000 tonnes of fish annually from 1960s through 1970s. Total fish catches decreased from a maximum of 7100 tonnes in 1967 to less than 500 tonnes by 1990s. Catch rates decreased from about 8 kg in the 1960s to less than 1 kg per net per night by 1975. During the 1970s the catch was dominated by Oreochromis niloticus (67%) followed by Clarias gariepinus (17%), and Protopterus aethiopicus (15.1 %). By 1990s the proportion of O. niloticus had decreased to 45.1% while that of P. aethiopicus had increased to 37.6%. These changes seem to have been caused by overfishing resulting from increased fishing effort from the recommended 250 to about 1000 boats and the additional increase in effort through driving fish into the nets by beating water. The maximum size of O. niloticus in the fishery decreased from 32 cm total length in 1975/78 to 22 cm in 1988/92 while the size at first maturity decreased from about 21 cm to 14 cm during the period. This has been concurrent with a shift in the mesh size of gillnet used from 127 mm (5") in 1960s to 64 mm by 1990s. Environmental changes, especially in lake level in 1980, may also have affected the fishery.

大熊猫生殖生物学研究 I:大熊猫发情期血清和尿液中促黄体素、孕酮和17β-二雌二醇含量的变化

放射免疫和生物测定法表明3只大熊猫在发情高峰期均出现雌二醇和促黄体素高峰; LH峰值出现在E_(2)峰值以后。根据雌兽在发情期血清和尿液中这二种激素的变化可为选择人工授精最佳时间提供可靠的理论依据。图2表2参15