Different IVIG glycoforms affect in vitro inhibition of anti-ganglioside antibody-mediated complement deposition

Intravenous immunoglobulin (IVIG) is the first line treatment for Guillain–Barré syndrome and multifocal motor neuropathy, which are caused by anti-ganglioside antibody-mediated complement-dependent cytotoxicity. IVIG has many potential mechanisms of action, and sialylation of the IgG Fc portion reportedly has an anti-inflammatory effect in antibody-dependent cell-mediated cytotoxicity models. We investigated the effects of different IVIG glycoforms on the inhibition of antibody-mediated complement-dependent cytotoxicity. Deglycosylated, degalactosylated, galactosylated and sialylated IgG were prepared from IVIG following treatment with glycosidases and glycosyltransferases. Sera from patients with Guillain–Barré syndrome, Miller Fisher syndrome and multifocal motor neuropathy associated with anti-ganglioside antibodies were used. Inhibition of complement deposition subsequent to IgG or IgM autoantibody binding to ganglioside, GM1 or GQ1b was assessed on microtiter plates. Sialylated and galactosylated IVIGs more effectively inhibited C3 deposition than original IVIG or enzyme-treated IVIGs (agalactosylated and deglycosylated IVIGs). Therefore, sialylated and galactosylated IVIGs may be more effective than conventional IVIG in the treatment of complement-dependent autoimmune diseases.

A glance on recent progresses in diagnosis and treatment of primary immunodeficiencies

Primary immunodeficiencies (PIDs)* belong to the group of rare diseases which need more awareness by the relevant medical disciplines. Below a review on recent progresses in diagnosis and treatment of PIDs is given. Reducing the regrettable delay in diagnosis of PIDs (worldwide) is possible only when awareness is increased by doctors who may encounter patients with PID. This review shall serve this purpose. Progresses in understanding what the link might be between one genetic defect presenting in various phenotypes or how various gene defects may manifest by very similar PID phenotypes helps building awareness. Knowledge of PID favours early diagnosis, a cornerstone of optimal, sometimes life-long care at justifiable costs. The complexity of PIDs calls for clinical laboratory and clinical diagnostic performed by experts only. Exciting laboratory diagnostic progresses in early diagnosis of the most severe forms of PID are reviewed below. Progresses in curative therapies for PIDs, such as hematopoietic stem cell transplantation and gene therapies, are mentioned in short. About 80% of PID patients suffer from an antibody deficiency syndrome and can profit from non-curative replacement therapies with human immunoglobulin G concentrates. Modes of application, safety and hints for dosing of replacement therapies to reduce frequencies of severe infections are mentioned below. Thanks to the increasing quality of care, patients survive adolescence. A glance is given on the problems of transition to the adult medicine setting.

Die Juden in Memmingen : aus Anlaß der Einweihung der Memminger Synagoge

verf. von Julius Miedel

Denkschrift zum fünfundzwanzigjährigen Jubiläumsfeste des israelitischen Männervereins für Krankenpflege und Leichenbestattung dahier

im Auftrag des Ausschusses verfasst vom Vorstand Julius Spiegelthal

Mindeskrift i anledning af hundredaarsdagen for anordningen af 29. marts 1814 en fremstelling af jødiske rets-og livsforhold i udland og indland navnlig i tiden omkring aar 1800 : med an samling arkivalia

Julius Salomon og Josef Fischer. Udgivet af Danmark Loge u. O. B. No. 712

Die Revolution und die Juden in Ungarn : nebst einem Rückblick auf die Geschichte der Letzteren

von I. Einhorn. Bevorwortet von Julius Fürst

Die jüdischen Kolonien in Russland : kulturhistorische Studie und Beitrag zur Geschichte der Juden in Russland ; nach den Berichten des russisch-israelitischen Journals "Woschod" und nach eigenen in den russisch-jüdischen Kolonien selbst gemachten Beobachtungen bearbeitet

Julius Elk

Das Judenthum in seiner Vergangenheit und Gegenwart : mit besonderem Hinblick auf Ungarn

von Jul. Laźár