Executive Order Number Forty, November 1, 1990

The Governor's Alliance on Substance Abuse is the designed agency to receive Drug Control& System Improvement Grant Program funding.

Executive Order Number Forty One, November 6, 1990

Empowers the Governor to accept retrocession of Federal jurisdiction if offered by appropriate federal authorities.

Executive Order Number Forty Two, July 1991

Invokes across-the-board budget reductions as determinded by the Governor.

Executive Order Number Forty Three, April 30, 1992

Invokes further budget cuts as determinded by the Governor.

Executive Order Number Forty Four, April 30, 1992

Rescinds Executive Order #46 and recommits efforts of the Executive branch and all department directors, member of governing boards and commissions, and other public officers affirmative action, discriminatory harrassment prevention, and cultural diversity.

Executive Order Number Forty Five, August 27, 1992

Orders the Department of Econmic Development to work with the Regional Coordinating Council.

Executive Order Number Forty Six, January 5, 1993

Establish the TIM Council (Telecommunication and Informational Management)

Executive Order Number Forty Seven, March 29, 1993

Accepts offer of assignment of concurrent jurisdiction.

Executive Order Number Forty Eight, February 1994

Establish the Iowa Commission for National and Community Service

Executive Order Number Forty Nine, March 4, 1994

Establish the Telemedicine Advisory Council.

Executive Order Number Fifty, May 2, 1994

Establishs the Iowa Health Reform Transition Team. Rescinded by Executive Order #60.

Executive Order Number Sixty Eight, November 23, 1998

Prohabits use of all tobacco products in the areas of buildings under Governor's control in the State Capital Complex and all offices occupied by state government.

MAR elements and transposons for improved transgene integration and expression.

Reliable and long-term expression of transgenes remain significant challenges for gene therapy and biotechnology applications, especially when antibiotic selection procedures are not applicable. In this context, transposons represent attractive gene transfer vectors because of their ability to promote efficient genomic integration in a variety of mammalian cell types. However, expression from genome-integrating vectors may be inhibited by variable gene transcription and/or silencing events. In this study, we assessed whether inclusion of two epigenetic control elements, the human Matrix Attachment Region (MAR) 1-68 and X-29, in a piggyBac transposon vector, may lead to more reliable and efficient expression in CHO cells. We found that addition of the MAR 1-68 at the center of the transposon did not interfere with transposition frequency, and transgene expressing cells could be readily detected from the total cell population without antibiotic selection. Inclusion of the MAR led to higher transgene expression per integrated copy, and reliable expression could be obtained from as few as 2-4 genomic copies of the MAR-containing transposon vector. The MAR X-29-containing transposons was found to mediate elevated expression of therapeutic proteins in polyclonal or monoclonal CHO cell populations using a transposable vector devoid of selection gene. Overall, we conclude that MAR and transposable vectors can be used to improve transgene expression from few genomic transposition events, which may be useful when expression from a low number of integrated transgene copies must be obtained and/or when antibiotic selection cannot be applied.

Executive Order Number Fifty Two, July 1, 1994

Replaces and rescinds Executive Order #35.

Executive Order Number Fifty Three, July 29, 1994

Eastablish an Iowa Workforce Development Council.