长臂猿的DNA序列进化及其系统发育研究

测定了来自白眉长臂猿、黑冠长臂猿、白手长臂猿合趾长臂猿352-399bp的线粒体细胞色素b基因的序列。发现自然选择对该基因ＤＮＡ序列水平的进化具有显著的影响。结合前人的序列,构建了系统的长臂猿分子系统树。结果表明,长臂猿属可分为4组:(1)白眉长臂猿组,仅一个种;(2)合趾猿组,仅合趾猿一个种;(3)白手长臂猿组,包括白手长臂猿、敏长臂猿、穆氏长臂猿、黑帽长臂猿和克氏长臂猿;(4)黑冠长臂猿组,包括黑冠长臂猿。对应于形态学上的4个亚属。黑冠长臂猿亚种间的遗传分化程度显著高于白手长臂猿亚属种间的分化程度。结果提示,黑冠长臂猿可能至少应分为4个种,它们在保护上具有同等重要的地位,应分别加以保护。

On the optimum buoyancy-weight relation for a bottom trawl

Comparative fishing operations were carried out with 0.55; 0.75 and 1.00 buoyancy-weight relations of bottom trawl in order to find out the optimum relation. Two nets, 18.26 m. (60') two seam nylon and 16.16 m. (23') two seam cotton trawls, were used for the experiment. The results showed that the total catch per trawling hour with 0.75 B-W relation was 16.5% and 32.08% more than that with 0.55 and 1.00 B-W relation for nylon net used. A similar trend was noticed with cotton trawl also as the catch rate with 0.75 B-W relation was 13.89% and 25.78% more than that with 0.55 and 1.00 B-W relations. However, the analysis of catch composition indicated that the off bottom fishes like lactarius, upenoides sp., synagris sp. etc., were of more percentage with 1.00 B-W relation, near bottom fish like saurida, sciaenids etc., were more with 0.75 B-W relation, while the bottom fishes like soles, prawns, skates and rays etc., were more with 0.55 B-W relation.

On the resources of demersal fishes for bottom trawling in inshore waters off Kakinada by small mechanised boats

An attempt is made to assess the available resources of demersal fishes for bottom trawling off Kakinada, in inshore waters. From the experimental fishing operation during 1964-66, the average catch per hour was 52.79 kg for 9.13 m (30') OAL mechanised boat. The catch composition was dominated by prawns and sciaenids forming 45% of the total catch. The average catch per trawling hour was more during the quarter April - June. An assessment on productive depth range has indicated that catch rate is increasing with increase in the depth of fishing.

Genetic diversity and taxonomic implication of Cordyceps sinensis as revealed by RAPD markers

Random amplified polymorphic DNA (RAPD) markers are used to investigate genetic variation and evolutionary relationships of 29 samples of Cordyceps sinensis from different geographical populations on the Qinghai-Tibet plateau. Out of 137 RAPD bands scored, 100 are polymorphic. A correlation is revealed between geographical distance and genetic distance. The molecular phylogenetic tree suggests that the 29 samples are divided into three notable clusters, corresponding to the geographical populations, i.e., the north population (NP), middle population (MP), and south population (SP). The NP consists of 7 northern samples from Menyuan, Maqu, and Luqu, the MP consists of 8 samples from Yushu and Chengduo, and the SP consists of 14 samples from Byma Snow Mountain, Renzhi Snow Moutain, Chongcaoxiwa, and Dacaodi. It is demonstrated that extensive genetic diversity is found among different geographical populations of C. sinensis. The genetic diversity pattern of C. sinensis may be caused by the founder effects. The taxonomic status of NP, MP, and SP populations should be that they are different subspecies rather than different species.

Molecular phylogenetic systematics of twelve species of acipenseriformes based on mtDNA ND4L-ND4 gene sequence analysis

Acipenseriformes is an endangered primitive fish group, which occupies a special place in the history of ideas concerning fish evolution, even in vertebrate evolution. However, the classification and evolution of the fishes have been debated. The mitochondrial DNA (mtDNA) ND4L and partial ND4 genes were first sequenced in twelve species of the order Acipenseriformes, including endemic Chinese species. The following points were drawn from DNA sequences analysis: (i) the two species of Huso can be ascribed to Acipenser; (ii) A. dabryanus is the mostly closely related to A. sinensis, and most likely the landlocked form of A. sinensis; (iii) genus Acipenser in trans-Pacific region might have a common origin; (iv) mtDNA ND4L and ND4 genes are the ideal genetic markers for phylogenetic analysis of the order Acipenseriformes.

The Gjerstad kiln: a combined hot and cold smoking kiln

A combined hot and cold smoking kiln has been constructed for use at Lake Turkana, Kenya. Used for cold smoking, the kiln's main advantages over the traditional kilns used in this area are its greater smoking capacity and lower firewood consumption. Having the option of using the same smoke house for cold and hot smoking reduces the total construction costs for an operator producing both types of smoke cured product. The hot smoking process was found to require markedly less firewood than cold smoking, a fact of considerable importance in desert or semi-desert areas with sparse vegetation.

氨基酸残基可及性与蛋白质家族成员结构的保守性

在细胞色素c族蛋白和免疫球蛋白家族中一些蛋白质片段的序列比较和分析的基础上,通过计算其氨基酸残基的可及性,对残基可及性与蛋白质序列及其三维结构的保守性之间的关系进行了分析和探讨。结果表明,序列中凡是保守的残基,其可及性都较低,而且这些低可及性的保守性残基与维持蛋白质特有的三维结构相关。作者认为,同一家族的蛋白质中,在进化上相距较远的各成员之间,结构的保守性主要是体现在其三维结构上;序列中的保守性残基,对于维持整个蛋白质分子特定的三维结构和功能有着重要的意义;此外,序列中的保守性残基一般均位于其整个分子结构的内部,因而具有较低的可及性。鉴于“可及性”本身只具有相对的意义,故可及性较低的残基不一定是保守的,而保守的残基则是低可及性的。

High temperature processing of fish sausage 4. - Heat penetration study

An examination was made of the rate of penetration of heat into fish sausage during processing at 115.6°C. Findings showed processing for 24 minutes to bring about complete destruction of Clostridium botulinum. A processing time of 30 minutes destroys almost all spoilage-causing organisms, thus prolonging the shelf life of the products.

Reliability of Escherichia coli and faecal streptococci as indicators of Salmonella in frozen fishery products

The results obtained in the present study suggest that Escherichia coli and faecal streptococci are of little value as indicators of the possible presence of Salmonella in frozen fishery products.

Trophic State Index, morphoedaphic index and fish yield prediction in a sub-tropical lake, Manchar (Sindh), Pakistan

Limnological factors of a sub-tropical lake Manchar were studied on seasonal basis. The mean values of various parameters were: transparency, (secchi disc reading): 90.5 cm, Orthophosphate: 0.257 mg/l, TDS: 3310,5 mg/l, Conductivity: 5232 µs/l, Total Chlorophyll (Planktonic): 31.3 µg/l Planktonic biomass: 5466 µg/l. Trophic state index (TSI) was calculated by using Carlson's (1977) equations. Mean TSI for transparency was 61, while for orthophosphate and chlorophyll, it was 82 and 64 respectively. TSI values indicate advanced eutrophic state of Manchar Lake. Morphoedaphic index (MEI) was also calculated on seasonal basis. The mean values were, TDS: 1103, conductivity: 1744, alkalinity: 60, transparency: 29 and biomass (plankton dry weight): 1746. Fish yield prediction for Manchar Lake (Z =3m, mean area=100 km²) was calculated by using MEI values. The results were quite different among various parameters. Conductivity (89.1mt/y), biomass (67.6 mt/y) and TDS (44.6 mt/y) were found to be good predictors of fish yield. Chlorophyll, transparency and alkalinity values gave very low estimate.

大白蚁亚科云南省新记录种

描述了云南省大白蚁亚科新记录3个种,分别为近三型大白蚁、海南大白蚁,梯头大白蚁.

Synchronization in baroclinic systems

Synchronization of periodic and chaotic oscillations between two coupled rotating baroclinic fluid systems will be presented. The numerical part of the study involves a pair of coupled two-layer quasigeostrophic models, and the experimental part comprises two thermally coupled baroclinic fluid annuli, rotating one above the other on the same turntable. Phase synchronization and imperfect synchronization (phase slips) have been found in both model and experiments, and model simulations also exhibit chaos-destroying synchronization. © 2008 IOP Publishing Ltd.