Calagem e adubação fosfatada no crescimento de mudas de mimosa caesalpiniifolia benth. em latossolo vermelho-amarelo

This work aimed to evaluate the effects of liming and phosphate fertilizer for the production of sabiá (Mimosa caesalpiniifolia Benth.) seedlings without thorns under a greenhouse. Seedlings 10 days old were transferred to plastic bags containing 2.0 kg of psamitic Dystrophic Red-Yellow Latosol (Typic Haplustox) collected from 40 to 70 cm layer. The experiment was carried out in Teresina county, Piauí state, Brazil, from July to October of 2008. Two liming doses (with and without liming) and five phosphorus doses combined in a 2 x 5 factorial scheme were used. The experimental design used was the randomized blocks with four replications having each plot three seedlings. The calculated lime amount was enough to elevate the base saturation to 50 % and the phosphorus doses were: 0, 30, 60, 90, and 120 mg kg-1 of soil. One seedling per pot was cultivated and the pot dimension was 10 by 23 cm. The evaluated variables were height, diameter, leaves number, leaf area, and shoot and roots dry matter. For the studied soil condition, the liming is not necessary to produce 'Sabiá' seedlings. The application, on average, from 72 to 107 mg kg-1 of P promote, respectively, from 90 to100 % of maximum values of height, diameter, leaf area and shoots and roots biomass.

Micorrização e crescimento de progênies de hymenaea stignocarpa mart. ex. Hayne em subsolo de área degradada degraded area

In tropical ecosystems, little is known about the relationship between arbuscular mycorhizal fungi (AMF) and the host genetic variability, especially among tree species. This study aimed to examine the response of 19 progenies of jatobá-do-cerrado (Hymenaea stignocarpa Mart. Ex. Hayne) inoculated with AMF, the root colonization and seedlings early growth were evaluated, growing in cultivated in subsoil from the loading area, under greenhouse conditions. The seedlings, germinated in the laboratory, were transferred to plastic bags containing subsoil and sand mixture (4:1). For the inoculation, each replicate received 100 g of soil (with about 48 spores of AMF) from a preserved Cerrado area, to reintroduce microorganisms. After 120 days, the mycorrhiza colonization (COL), the number of spores of AMF, the plant height (PH), the weight of shoot dry matter (SDM) and the root fresh matter (RFM) were assessed. The highest values of COL, SDM and RFM were observed in the progenies JC7, JC18, JC29, JC27 and JC14; the JC7 andJC18 also increased number of spores. There were significant and positive correlations between COL and the others variables (AP, NE, RFM and SDM, and between RFM and other variables (NE, AP and SDM). The conclusion is that, there is variability among the genotypes of Hymenaea stignocarpa growth (AP MFSR and DMAP) and mycorrhization (COL and NE), with emphasis on two progenies (JC7 and JC18), that showed the highest values, which can be resulted of a greater affinity to strains of AMF- soil inoculum.

Global analysis of the sugarcane microtranscriptome reveals a unique composition of small RNAs associated with axillary bud outgrowth

Axillary bud outgrowth determines shoot architecture and is under the control of endogenous hormones and a fine-tuned gene-expression network, which probably includes small RNAs (sRNAs). Although it is well known that sRNAs act broadly in plant development, our understanding about their roles in vegetative bud outgrowth remains limited. Moreover, the expression profiles of microRNAs (miRNAs) and their targets within axillary buds are largely unknown. Here, we employed sRNA next-generation sequencing as well as computational and gene-expression analysis to identify and quantify sRNAs and their targets in vegetative axillary buds of the biofuel crop sugarcane (Saccharum spp.). Computational analysis allowed the identification of 26 conserved miRNA families and two putative novel miRNAs, as well as a number of trans-acting small interfering RNAs. sRNAs associated with transposable elements and protein-encoding genes were similarly represented in both inactive and developing bud libraries. Conversely, sequencing and quantitative reverse transcription-PCR results revealed that specific miRNAs were differentially expressed in developing buds, and some correlated negatively with the expression of their targets at specific stages of axillary bud development. For instance, the expression patterns of miR159 and its target GAMYB suggested that they may play roles in regulating abscisic acid-signalling pathways during sugarcane bud outgrowth. Our work reveals, for the first time, differences in the composition and expression profiles of diverse sRNAs and targets between inactive and developing vegetative buds that, together with the endogenous balance of specific hormones, may be important in regulating axillary bud outgrowth. © 2013 © The Author(2) [2013].

Nutrição e produtividade do crambe em razão da adubação de semeadura na safrinha

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Pulp fruit added to culture medium for in vitro orchid development

As an additive in in vitro culture media, fruits have a great potential for facilitating economical orchid production because of lower technology requirements and the ease of obtaining raw materials to formulate culture media. We studied the in vitro growth of Cattleya bicolor Lindl. grown in a simplified culture medium supplemented with different kinds of fruit pulp. The experimental design was completely randomised, with eight seedlings per replication and ten replications per treatment, for a total of 80 seedlings per treatment. The culture medium was made using 150 g L -1 of pulp (without peel or seed) from the following fruits: ripe Santa Cruz tomatoes (Solanum lycopersicum L.), dwarf bananas (Musa cavendishii L.) of intermediate ripeness, light green chayote (Sechium edule (Jacq.) Sw), ripe papaya (Carica papaya L.) or green coconut (Cocos nucifera L.).The treatment control was MS 50 %. The treatments and the control were kept in a growth chamber for seven months before evaluating seedling survival percentage, shoot height, number of leaves, rooting percentage, root number, root length and dry masses of shoot and roots. The highest percentages of seedling survival were obtained using MS 50 %, banana and coconut medium. The seedling survival and rooting percentages illustrate that it is possible to emphasise the culture medium MS 50% and the culture medium supplemented with coconut on the most traditional culture medium with banana or tomato pulp. For the in vitro development of Cattleya bicolor Lindl., a simplified culture medium supplemented with coconut pulp is the most suitable for use as an alternative to MS 50%. A simplified culture medium supplemented with papaya pulp is not recommended for the in vitro development of Cattleya bicolor Lindl.

Tolerance of physic nut plants to aluminum activity in nutrient solution

Plants have different levels of tolerance to phytotoxic effects of aluminum and the exploitation of this characteristic is of significant importance to the use of acid soils. This research aimed to evaluate the effect of aluminum activity in nutrient solution on growth of physic nut young plant. After seven days of adaptation, plants were submitted to Al concentrations of 0; 200; 400; 600; 800 and 1,000 μmol L-1, corresponding to Al3+ activity solution, of: 14.5, 21.4; 46.6; 75.6; 108.3 e 144.8 μmol L-1, respectively. The increased activity of Al3+ decreased linearly the number of leaves, plant height, leaf area, shoot dry matter and root length of physic nut plant. Physic nut young plants are sensitive to high aluminum activity in solution. The root length, number of leaves, shoot dry matter and total dry matter were variables more affected by Al activity in solution, and can be used to discriminate the tolerance levels to aluminum in physic nut plants. The accumulation of aluminum increased in a activity-dependent manner; however, its translocation from root to shoot was low.

Evaluation of ornamental grasses submitted to water stress

The work was carried out at the College of Agricultural and Veterinary Sciences of the State University of São Paulo (UNESP/FCAV), Campus of Jaboticabal, Brazil, aiming to study the tolerance response to water stress and capacity of regeneration after mowing three different ornamental grasses used in Brazilian landscaping: Imperial zoysia grass (Zoysia japonica 'Imperial'), zoysia grass (Zoysia japonica) and St. Augustine grass (Stenotaphrum secundatum). The experimental design was entirely randomized in a factorial scheme 33 (three grass species: Zoysia japonica 'Imperial', Zoysia japonica and Stenotaphrum secundatum; in three water stress conditions: under full sun, with and without irrigation, and under greenhouse conditions without irrigation) with four replications per plot. The irrigation was performed using microsprinklers with a flow of 0.28 L s-1, and the grasses of all plots were mowed monthly. The evaluations were executed monthly, before mowing the grass, in the beginning of each season, that means, in October (for Spring evaluation), January (for Summer), April (for Autumn) and July (for Winter), considering the Brazilian climate conditions. The evaluated parameters were shoot height and total dry mass. The data were submitted to the variance analysis and the means were compared by the Tukey test at 5% confidence level. The grasses grown under greenhouse conditions, without irrigation, showed higher height and lower dry mass weight averages, what possibly indicates that the plants etiolated. The grasses grown under full sun, either with or without irrigation, showed a similar plant development. The S. secundatum species showed greater tolerance to water stress in October, month that followed the longest dry period. The total dry mass was gradually reduced during the experiment for all grasses grown under greenhouse conditions without irrigation; however, a great general tolerance to water stress was observed for all grasses because all of them survived along nine months without irrigation.

Cryopreservation, early seedling development, and genetic stability of Oncidium flexuosum Sims

An efficient cryopreservation protocol was developed for mature seeds of Oncidium flexuosum Sims. Seed morphology, protocorm formation, and early seedling development were also assessed. The effects of phloroglucinol and Supercool X-1000® as cryoprotectant additives in the vitrification solution were investigated. Dehydration using the plant vitrification solution 2 (PVS2) for 60 and 120 min prior to immersion in liquid nitrogen promoted the highest frequency of in vitro seed germination 6 weeks following culture on half-strength Murashige and Skoog (1/2 MS) medium. Mature seeds submitted to vitrification for 120 min in PVS2 and 1 % phloroglucinol at 0 °C enhanced germination by 68 %, whereas in PVS2 and 1 % Supercool X-1000® germination was just moderately enhanced (26 %). In vitro-germinating seedlings developed healthy shoots and roots without the use of plant growth regulators. After 6 months of growth, there were no differences between in vitro- and ex vitro-grown seedlings for various phenotypic characteristics, including shoot length, number of leaves, number and length of roots, and fresh and dry weight. Seedlings were transferred to greenhouse conditions and successfully acclimatized, further developing into normal plants with over 90 % survival. Comparative analysis of seedlings from control and vitrified seeds using flow cytometry indicated that no change in ploidy levels occurred as a result of cryopreservation, therefore maintaining seedlings genetic stability. In this study, vitrification with PVS2 for 120 min with the addition of 1 % phloroglucinol offers a simple, safe, and feasible protocol for cryopreservation of O. flexuosum mature seeds. © 2013 Springer Science+Business Media Dordrecht.

Deposição de sílica e teor de nitrogênio e silício em arroz

The nitrogen fertilization is an important practice to reach high productivity, however, nitrogen high level can cause lodging and make the plants more sensitive to disease. Numerous studies has demonstrate that a lot of grasses accumulate silicon at its tissues, and the biggest part of this element is deposited on the leaf, that would work like a mechanical barrier to diseases come in. However, high levels of nitrogen can reduce the silica deposition at the plants. The objective of this study was to evaluate silicon and nitrogen content in shoot and silica deposition in upland rice leaf as a result of Si and N levels. The experimental design used was completely randomized in factorial schema 3 x 2 with five replications. The treatments consisted from levels of N (5, 75 and 150 mg dm-3 of soil) in urea form and two levels of SiO2 (0 e 400 mg dm-3) in calcium silicate form (Wollastonita). The increased of urea fertilization reduced the silicon content of rice plants and the silica deposition at the external cells wall the epidermal rice leafs.

Growth of euterpe oleracea seedlings under different color nets and light conditions

Euterpe oleracea, a native palm to the Amazon, has recognized trading importance because of its fruits and heart of palm. However, it still requires general information on its propagation and cultivation. As light is essential for plant growth, this work aimed to study the effect of different color nets and light conditions on the initial growth of E. oleracea, with the objective to obtain high quality seedlings. The experiment was conducted in Jaboticabal County, São Paulo State, Brazil. The experimental design was entirely randomized, with six treatments (six light conditions: red net with 50% of shading - red Chromatinet®; blue net with 50% of shading - blue Chromatinet®; black nets with 70, 50 or 30% of shading; and full sun) and six replications (six evaluation periods: 30, 60, 90, 120, 150 and 180 days after the beginning of the experiment). Each plot consisted of four pots with one plant per pot, resulting in 144 plants. Seedling growth was monthly evaluated by collecting four plants per replication until the end of the experiment. Shoot length, root length, stem diameter, leaf number, leaf area and root and shoot dry matter were evaluated. The best quality seedlings of E. oleracea were obtained when they were cultivated under the black net with 50% of shading.

Growth regulators for in vitro propagation of Tillandsia gardneri (Bromeliaceae)

Tillandsia gardneri Lindi, is a herbaceous perennial with ornamental value. However, in Brazil there is no report about this species' cultivation on a commercial scale. The low multiplication rate of T. gardneri (in average one offshoot/plant/year) leads to illegal over-collection in the wild to meet commercial demands. The development of protocols for in vitro propagation of T. gardneri may be useful for increasing multiplication rate, producing enough plants to supply the ornamental market and also to reduce the pressure over plants collection in the wild. The present study evaluated the effect of growth regulators (6-benzylaminopurine-BA alone or in combinations with naphthaleneacetic acid-NAA) on shoots development from seedlings pre-established in vitro, from seed germination on 1/4 MS medium without growth regulators. Seedlings (with about 1.0 cm long) were re-cultured to solid 1/2 MS media supplemented with growth regulators. After 30 days on the induction medium seedlings were re-cultured to MS basal medium. The experiment was conducted in a complete randomized design with four replications and ten treatments: control (free of growth regulators), BA (0.5, 1.0 and 2.0 mg/L), BA (0.5 mg/L) + ANA (0.1 mg/L), BA (1.0 mg/L) + ANA (0.1 mg/L), BA (1.0 mg/L) + ANA (0.5 mg/L), BA (2.0 mg/L) + ANA (0.1 mg/L), BA (2.0 mg/L) + ANA (0.5 mg/L), and BA (2.0 mg/L) + ANA (1.0 mg/L). The outgrowth of shoots did not occur on medium devoid of growth regulators (control). Regression analysis for some evaluated parameters, such as percentage of seedlings responsive to shoot formation and number of shoots/seedling, and regulators concentrations (BA or ANA) were significant, allowing the establishment of the growth regulators concentration for obtaining the best multiplication rate. Some seedlings maintained in media with ANA (0.5 or 1.0 mg/L) were completely converted into callus masses that turned dark brown leading to seedlings death.

Danos do complexo broca-podridão à produtividade e à qualidade da cana-de-açúcar fertirrigada com doses de nitrogênio

The objective of this work was to evaluate the effect of nitrogen-fertigation on the damage caused by the borer-rot complex to the productivity and quality of fertigated sugarcane. The experiment was carried out in field, using a randomized complete block design, with four replicates and five N doses (0, 50 100, 150, and 200 kg ha-1) applied through drip irrigation. The following variables were determined: number of holes caused by Diatraea saccharalis (Lepidoptera: Crambidae); number of internodes infected with red rot; incidence percentage of red rot; stalk productivity; and sugar content. In laboratory, the attractiveness and the consumption of culm fragments by fourth instar caterpillars, in trials with or without choice, were evaluated. Nitrogen fertigation increased the incidence of D. saccharalis and red rot. The number of holes produced by the larvae was correlated with the occurrence of red rot. Sugar percentage reduced with the increase in the number of red rot affected internodes. Nitrogen doses did not affect the attractiveness of sugarcane culm fragments; however, higher N doses increased the consumption of these fragments by D. saccharalis caterpillars. Despite the damages to quality, nitrogen fertilization expressively enhanced the productivity of stalks and sugar.

Soil organic matter and physical attributes affected by crop rotation under no-till

Growing cover crops in systems under no tillage affects different pools of soil organic matter, and eventually soil physical attributes are modified. The objective of this study was to evaluate changes in soil organic matter and their relationship with soil physical attributes as affected by plant species grown in rotation with soybean [Glycine max (L.) Merr.] under no-till for 3 yr. Crop rotations included grain sorghum [Sorghum bicolor (L.) Moench], ruzigrass [Urochloa ruziziensis (R. Germ, and CM. Evard) Crins] and sorghum mixed with ruzigrass, all grown in fall/winter, followed by pearl millet [Pennisetum americanum (L.) Leeke], sunn hemp (Crotalaria juncea L.) and sorghum-sudangrass [S. bicolor × S. sudanense (Piper) Stapf] grown during the spring, plus a fallow check plot. Soybean was grown as the summer crop. Millet and sorghum-sudangrass cropped in spring showed higher root and shoot production as spring cropping. In fall/winter, sorghum mixed with ruzigrass yielded higher phytomass compared with sole cropping. Soil physical attributes and organic matter fractioning were positively affected by cropping millet and sorghum-sudangrass whereas intermediate effects were observed after sunn hemp. Maintaining fallow in spring had negative effects on soil organic matter and physical properties. Ruzigrass and sorghum mixed with ruzigrass cropped in fall/winter resulted in better soil quality. Spring cover crops were more efficient in changing soil bulk density, porosity, and aggregates down to 0 to 10 cm; on the other hand, fall/winter cropping showed significant effects on bulk density in the uppermost soil layer. Total C levels in soil were increased after a 3-yr rotation period due to poor initial physical conditions. Fractions of particulate organic C, microbial C, and C in macroaggregates were the most affected by crop rotations, and showed high relation with improved soil physical attributes (porosity, density, and aggregates larger than 2 mm). © Soil Science Society of America, All rights reserved.

Sistemas de manejo e efeito residual do potássio na produtividade e nutrição do feijão-caupi

Cowpea [Vigna unguiculata (L) Walp] have great social and economic importance for the Para State. It grows well in areas with low precipitation and two crop cycles can be obtained annually. This study aimed to assess the effect of the residual fertilization from a previous culture (Sorghum bicolor L. Moench) and crop systems on cowpea yield and macronutrient concentration on leaves of three cowpea cultivars (BRSMilênio, BRS-Urubuquara e BRS-Guariba). The study was conducted at the UFRA. The treatments were two crop systems (minimum tillage and conventional), four levels of potassium (50, 100, 200 e 300 kg de KCl ha-1 applied to a previous sorghum culture) and the three cowpea cultivars. Treatments were organized as a three (4 x 2 x 3) factor experiment on a randomized complete block design. The soil was a yellow latosol. In each experiment plot five plants were selected to determine shoot dry matter and foliar nutrient concentration. Grain yield was determined after harvesting all plants on the experiment plot. The residual KCl fertilization affected foliar nutrient content, but did not affect shoot dry mass or yield of grain. Yield was higher in the minimum tillage system. Highest yield (1590 kg ha-1) was recorded int the cv. 'Guariba' when 100 kg of KCl ha-1 had been used in the previous crop. The highest content of leaf N and K was found in cowpea under minimum tillage system. The amount of P and Mg were higher in the conventional system whereas the amount of Ca did not change.

Comportamento do noni à salinidade da água de irrigação em solo com biofertilizante bovino

Salt excess in soil and water used for irrigation can cause significant loss of production and growth in cultivated plants. Among some options for reduction of negative effects of salts to plants in cultivated areas, fermented bio fertilizer has been used to grow vegetables and fruit tree irrigated with saline water. The study aimed at evaluating the behavior of the noni plant to salinity of the irrigation water in substrate with and with no bio fertilizer. Treatments were arranged in a randomized block design with four replications, using a 5 × 2 factorial arrangement. Five levels of electrical conductivity of irrigation water (0.5, 1.5, 3.0, 4.5, 6.0 dS m-1) were used in substrates with and with no bio fertilizer. Parameters were evaluated as follows: plant height, stem diameter, number of leaves, leaf area, shoot dry matter and water consumption. All evaluated variables were negatively affected by the increase in salt concentration of the irrigation water, but always with less intense effects in treatments with bio fertilizer.The bio fertilizer does not eliminate, but mitigates the negative effects of salts in noni plants.