Produção de Brachiaria brizantha e Panicum maximum com milho e adubação nitrogenada

In places characterized with high temperatures and rain occurrence in great intensity in the summer, but with dry winter, the major limitations for the sustainability of no tillage systems are low production of straw during fall-spring period and the fast decomposition during the rain season. To try to solve the problem, intercropped cultures of grains with forage species has presented reliable results; because offer vegetal covering to the next sowing, giving sustainability to the no tillage system. However, being a recent technology, its needed further studies in different areas involved for this system of production. Thus, this study had the objective 1) to evaluate the production of corn grain at different periods of intercropping with Brachiaria brizantha and Panicum maximum in no tillage system, and 2) aimed to evaluate the performance of forage at different periods of intercropping and the responses to nitrogen fertilization after the harvest of the corn, assessing mass productivity and quality. The experiment was carried out at the Lageado experimental farm, School of Agricultural Sciences, Botucatu campus belonged to São Paulo State University (UNESP) in structuralized Red Nitosol (Afisol). The experimental design was randomized blocks with four replications. The treatments were composed for four systems of no tillage involving corn: 1) single corn; 2) corn with Brachiaria brizantha cv. Marandu intercropped in the sowing; 3) corn with Brachiaria brizantha cv. Marandu intercropped with the fertilization of covering; 4) corn with Panicum maximum cv. Mombaça intercropped in the sowing and 5) corn with Panicum maximum cv. Mombaça intercropped with the fertilization of covering. After the harvest of the corn, it was applied, in equivalent quantities of nitrogen, ammonium nitrate in covering in doses of 0, 30, 60 and 120 kg ha-1, determining the forage mass productivity and quality. The simultaneous tillage of corn with P. maximum cv. Mombaça in the sowing compromises the grain productivity. When sowed in intercropping, B. brizantha presents a fiber concentration reduction and greater TDN concentration during the fall-spring period. Regarding benefits of intercropped cultures seeking to use in systems of production like agriculture-pasture integration, the best intercropping to be utilized is corn sown simultaneously with B. brizantha cv. Marandu.

Lichen metabolites modulate hydrogen peroxide and nitric oxide in mouse macrophages

The activities of perlatolic acid (1), atranorin (2), and lecanoric acid (3) and their derivatives, such as orsellinates and β-methyl orsellinates obtained by alcoholysis, were assessed for stimulation of the release of hydrogen peroxide and nitric oxide in cultures of peritoneal macrophage cells from mice. The hydrogen peroxide production was estimated by oxidation of phenol red, while the Griess reagent was used to determine the nitric oxide production. 1 and 4-methoxy-ethyl orsellinate (XVII) were the compounds that induced the greatest release of H 2O 2, whereas n-pentyl orsellinate (IV), iso-propyl orsellinate (V), sec-butyl orsellinate (VI), and XVII induced a small release of NO. These results indicate that lichen products and their derivatives have potential immune-modulating activities. © 2009 Verlag der Zeitschrift für Naturforschung, Tübingen.

Estudo da mcrobiota fúngica gastrintestinal de morcegos (Mammalia, Chiroptera) da região noroeste do estado de São Paulo: Potencial zoonótico

Bats are hosts of a rich diversity of microorganisms. Many studies indicate a close link between bats and fungi with pathogenic potential, especially for living in environments such as caves, caverns and hollow trees, favorable to the maintenance and spread of fungi. The objective was to study the gastrointestinal mycoflora of bats. Of the 98 samples belonging to 11 species of bats coming from 15 studied cities, 20% of the species were Carollia perspicillata, 19% Artibeus lituratus, 17% Molossus rufus, 13% Glossophaga soricina, 9% Nyctinomops macrotis, 8% Molossus molossus, 7% Desmodus rotundus, 2% Lasiurus ega and 1% Eptesicus furinalis, Myotis nigricans and Tadarida brasiliensis. The genus Aspergillus sp. was isolated from 29% of the samples, followed by 6% Microsporum sp. and Penicillium sp. 4% Trichophyton sp. and zygomycetes and 2% Fusarium sp. Of yeast species, 14% were from Rhodotorula sp., 10% Candida sp. and 2% Cryptococcus sp., 22% of isolates remained unidentified. All 82 cultures of organs were negative for Histoplasma capsulatum. There was a statistically significant association between the results of microbiological culture and bat species (p < 0.05). We conclude that the bats can act as disperser agents of fungi with pathogenic potential, although other studies should be performed to establish strategies to identify the main factors correlated with the growth and spread of microorganisms in nature and implication of bats in the epidemiological cycle.

Porphyromonas gingivalis LPS stimulation downregulates DNMT1, DNMT3a, and JMJD3 gene expression levels in human HaCaT keratinocytes

Objective: The role of epigenetic regulation in inflammatory diseases such as periodontitis is poorly known. The aim of this study was to assess whether Porphyromonas gingivalis lipopolysaccharide (LPS) can modulate gene expression levels of the some enzymes that promote epigenetic events in cultures of the human keratinocytes and gingival fibroblasts. In addition, the same enzymes were evaluated in gingival samples from healthy and periodontitis-affected individuals. Materials and methods: Primary gingival fibroblast and keratinocyte (HaCaT) cultures were treated with medium containing P. gingivalis LPS or P. gingivalis LPS vehicle for 24 h. After this period, cell viability was assessed by MTT test and total RNA extracted to evaluate gene expression levels of the following enzymes by qRT-PCR: DNA methyltransferase 1 (DNMT1), DNA methyltransferase 3a (DNMT3a), histone demethylases Jumonji domain containing 3 (JMJD3) and ubiquitously transcribed tetratricopeptide repeat, X chromosome (UTX). To evaluate gene expression in healthy and periodontitis-affected individuals, total RNA was extracted from biopsies of gingival tissue from healthy and periodontitis sites, and gene expression of DNMT1, DNAMT3a, JMJD3, and UTX was evaluated by qRT-PCR. Results: No significant differences were found in the gene expression analysis between healthy and periodontitis-affected gingival samples. The results showed that LPS downregulated DNMT1 (p < 0. 05), DNMT3a (p < 0. 05), and JMJD3 (p < 0. 01) gene expression in HaCaT cells, but no modulation was observed in gingival fibroblasts. Conclusion: P. gingivalis LPS exposure to human HaCaT keratinocytes downregulates gene expression of the enzymes that promote epigenetic events. Clinical relevance: The advance knowledge about epigenetic modifications caused by periodontopathogens may to possibly led to the development of new periodontal therapies. © 2012 Springer-Verlag.

Expressão do gene FOXE1 em culturas de osteossarcoma canino

Pós-graduação em Medicina Veterinária - FMVZ

Estudos de adsorção do ácido clavulânico

Pós-graduação em Biotecnologia - IQ

Aspectos higiênicos-sanitários de vegetais minimamente processados e perfil de resistência das leveduras isoladas frente ao hipoclorito de sódio e ozônio

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Liberação das enzimas LDH e PepX e evolução da maturação de queijo parmesão adicionado de culturas autolíticas de Lactobacilus helveticus

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito do suco de laranja e da glicosil hesperidina sobre o sistema imune inato de camundongos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Detecção de línfócitos T no intestino de frangos de corte tratados com lactobacillus spp. e desafiados com Salmonella enteritidis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Impacto das condições climáticas sobre a etiologia das bacteremias nosocomiais no Hospital das Clínicas da Faculdade de Medicina de Botucatu-UNESP

Pós-graduação em Doenças Tropicais - FMB

Citotoxicidade e expressão gênica de fibrobastos pulpares tratados com extratos de monômeros resinosos e derivados vegetais

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Doses de nitrogênio na cultura antecessora e do milho em sistema plantio direto

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Modelos matemáticos aplicados a problemas na cultura da cana-de-açúcar e no aproveitamento da energia da biomassa

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise econômica da produção de feijão, milho e soja com e sem irrigação no município de Itaí-SP

Pós-graduação em Agronomia (Energia na Agricultura) - FCA