Development of an ex vivo organ culture model using human gastro-intestinal tissue and Campylobacter jejuni.

Campylobacter jejuni is an important food-borne pathogen. However, relatively little is understood regarding its pathogenesis, and research is hampered by the lack of a suitable model. Recently, a number of groups have developed assays to study the pathogenic mechanisms of C. jejuni using cell culture models. Here, we report the development of an ex vivo organ culture model, allowing for the maintenance of intestinal mucosal tissue, to permit more complex host-bacterium interactions to be studied. Ex vivo organ culture highlights the propensity for C. jejuni to adhere to mucosal tissue via the flagellum, either as discrete colonies or as multicellular units.

A Novel Counter Sheet-Flow Sandwich Cell Culture Device For Mammalian Cell Growth In Space

Cell culture and growth in space is crucial to understand the cellular responses under microgravity. The effects of microgravity were coupled with such environment restrictions as medium perfusion, in which the underlying mechanism has been poorly understood. In the present work, a customer-made counter sheet-flow sandwich cell culture device was developed upon a biomechanical concept from fish gill breathing. The sandwich culture unit consists of two side chambers where the medium flow is counter-directional, a central chamber where the cells are cultured, and two porous polycarbonate membranes between side and central chambers. Flow dynamics analysis revealed the symmetrical velocity profile and uniform low shear rate distribution of flowing medium inside the central culture chamber, which promotes sufficient mass transport and nutrient supply for mammalian cell growth. An on-orbit experiment performed on a recovery satellite was used to validate the availability of the device.

Conferencia "Culture, Freedom and Christianity", presentada por Alberto de Onaindia en el Congress for Cultural Freedom, celebrado en Berlín en 1950.

Fecha: 2-7-1950 / Unidad de instalación: Carpeta 45 - Expediente 2-27 / Nº de pág.: 6 (mecanografiadas)

Danka Multimedia: Una sociedad cooperativa en expansión en la nueva economía digital

[ES] Danka Multimedia es una sociedad cooperativa que ofrece servicios de comunicación integral en el ámbito de marketing y la formación mediante el uso de las nuevas tecnologías. Por el sector de actividad en el que se encuentra, así como por su propia vocación empresarial, Danka ha experimentado desde sus inicios un proceso paulatino de crecimiento. Este crecimiento está siendo cada más vez progresivo y, por ello, la empresa se está viendo obligada a hacer frente a una serie de problemas derivados del mismo. Estos conflictos derivados del crecimiento, comunes a todo tipo de empresa, se ven acrecentados en el caso de Danka Multimedia por su personalidad jurídica, los valores y cultura que ello conlleva y el sector de actividad en el que desarrolla su actividad. La gestión de este crecimiento, por parte de Danka Multimedia, es el tema central que se aborda a lo largo del caso.

Morals in business organizations: an approach based on strategic value and strength for business management

[EN] In the last decades, the topic of business ethics has attracted great interest at the academic and professional levels. Nowadays business ethics is being increasingly implemented as a necessary discipline in universities’ study plans on business management. Moreover, its importance is also evident according to the worldwide increase of organizations and/or institutions that have implemented ethics systems. However, some approaches thoroughly do not consider the importance and the need of an ethical behaviour and are still guiding the actions and the way of thinking of many academics and professionals led to consider that the only responsibility of business is limited just to profit maximization.

Plano de implantação de uma arquitetura orientada a serviços - SOA - na Câmara dos Deputados

Apresenta um plano para implantação de uma Arquitetura Orientada a Serviços "SOA", na Câmara dos Deputados - Brasil. O plano de implantação SOA foi estruturado em dois projetos: "Entender SOA" e "Implantar SOA", descritos em suas macro atividades e foi embasado em um arcabouço teórico fundamentado em significativa literatura pesquisada que incluiu os modelos de referência e de arquitetura SOA disponíveis. Foram abordados, além dos aspectos tecnológicos, as questões de governança de TI e questões de cultura organizacional que precisam ser consideradas em projetos dessa natureza, considerando que SOA não é simplesmente uma questão de tecnologia, mas fundamentalmente uma questão de negócio e governança empresarial. Os aspectos de TI também foram explorados de forma suficiente para que as equipes técnicas tenham condições de implementar os princípios de desenho da arquitetura SOA.

What is fish culture?

Document contains 4 pages.

Evaluation of Waterhyacinth and Paddy Straw Waste for Culture of Oyster Mushrooms

Waterhyacinth ( Eichhornia crassipes (Mart.) Solms.) was evaluated at ratios of 25, 50 and 75% with paddy straw ( Oryza sativa L.) for oyster mushroom ( Pleurotus sajor-caju) cultivation. There was an increase in yield with decreasing ratio waterhyacinth.