Upregulation of acid-sensing ion channel ASIC1a in spinal dorsal horn neurons contributes to inflammatory pain hypersensitivity

Development of chronic pain involves alterations in peripheral nociceptors as well as elevated neuronal activity in multiple regions of the CNS. Previous pharmacological and behavioral studies suggest that peripheral acid-sensing ion channels (ASICs) cont

Corner effect and asymmetry in transonic channel flows

An experimental and numerical investigation into transonic shock/boundary-layer interactions in rectangular ducts has been performed. Experiments have shown that flow development in the corners of transonic shock/boundary-layer interactions in confined channels can have a significant impact on the entire flowfield. As shock strength is increased from M∞ = 1:3 to 1.5, the flowfield becomes very slightly asymmetrical. The interaction of corner flows with one another is thought to be a potential cause of this asymmetry. Thus, factors that govern the size of corner interactions (such as interaction strength) and their proximity to one another (such as tunnel aspect ratio) can affect flow symmetry. The results of the computational study show reasonable agreement with experiments, although simulations with particular turbulence models predict highly asymmetrical solutions for flows that were predominantly symmetrical in experiments. These discrepancies are attributed to the tendency of numerical schemes to overprediction corner-interaction size, and this also accounts for why computational fluid dynamics predicts the onset of asymmetry at lower shock strengths than in experiments. The findings of this study highlight the importance of making informed decisions about imposing artificial constraints on symmetry and boundary conditions for internal transonic flows. Future effort into modeling corner flows accurately is required. Copyright © 2011 by the American Institute of Aeronautics and Astronautics, Inc. All rights reserved.

Contact and channel modelling using part and function libraries in a function-based design approach

To support the development and analysis of engineering designs at the embodiment stage, designers work iteratively with representations of those designs as they consider the function and form of their constituent parts. Detailed descriptions of "what a machine does" usually include flows of forces and active principles within the technical system, and their localization within parts and across the interfaces between them. This means that a representation should assist a designer in considering form and function at the same time and at different levels of abstraction. This paper describes a design modelling approach that enables designers to break down a system architecture into its subsystems and parts, while assigning functions and flows to parts and the interfaces between them. In turn, this may reveal further requirements to fulfil functions in order to complete the design. The approach is implemented in a software tool which provides a uniform, computable language allowing the user to describe functions and flows as they are iteratively discovered, created and embodied. A database of parts allows the user to search for existing design solutions. The approach is illustrated through an example: modelling the complex mechanisms within a humanoid robot. Copyright © 2010 by ASME.

Nearest neighbour decoding and pilot-aided channel estimation in stationary Gaussian flat-fading channels

We study the information rates of non-coherent, stationary, Gaussian, multiple-input multiple-output (MIMO) flat-fading channels that are achievable with nearest neighbour decoding and pilot-aided channel estimation. In particular, we analyse the behaviour of these achievable rates in the limit as the signal-to-noise ratio (SNR) tends to infinity. We demonstrate that nearest neighbour decoding and pilot-aided channel estimation achieves the capacity pre-logwhich is defined as the limiting ratio of the capacity to the logarithm of SNR as the SNR tends to infinityof non-coherent multiple-input single-output (MISO) flat-fading channels, and it achieves the best so far known lower bound on the capacity pre-log of non-coherent MIMO flat-fading channels. © 2011 IEEE.

Is the assumption of perfect channel-state information in fading channels a good assumption?

Fading channels, which are used as a model for wireless communication, are often analyzed by assuming that the receiver is aware of the realization of the channel. This is commonly justified by saying that the channel varies typically slowly with time, and the receiver is thus able to estimate it. However, this assumption is optimistic, since it is prima facie not clear whether the channel can be estimated perfectly. This paper investigates the quality of this assumption by means of the channel capacity. In particular, results on the channel capacity of fading channels are presented, both when the receiver is aware of the realization of the channel and when it is aware only of its statistics. A comparison of these results demonstrates that information- theoretic analyses of fading channels that are based on the assumption that the receiver is aware of the channel's realization can yield helpful insights, but have to be taken with a pinch of salt. ©2009 IEEE.

A hot channel

This paper studies on-chip communication with non-ideal heat sinks. A channel model is proposed where the variance of the additive noise depends on the weighted sum of the past channel input powers. It is shown that, depending on the weights, the capacity can be either bounded or unbounded in the input power. A necessary condition and a sufficient condition for the capacity to be bounded are presented. © 2007 IEEE.

Plankton identified in stomach contents of Oreochromis niloticus (Pisces, Cichlidae) and the water system of Lakes Edward, George, and Kazinga channel - Uganda

The samples were collected from Lake Edward at Rwenshama, Kisenyi and Katwe, and from Lake George at Mahyoro, Kashaka and Kasenyi and in Kazinga Channel at Katunguru. The organisms identified from the water samples obtained irrespective of station or depth were mainly the phytoplankton (diatoms, blue-green algae and green algae). Of the phytoplankton, blue green-algae were the most abundant both in quantity and number of species especially in L. George. In order of importance were Microcystis spp, Planktolyngbya spp and Anabaenopsis spp were the dominant blue greens. Diatoms and green algae were present but less abundant. The estimated proportions of different types of phytoplankton identified in O. niloticus stomach contents indicate that bluegreen algae were the most abundant followed by the diatoms and green algae.

Channel selective tunnelling through a nanographene assembly.

We report selective tunnelling through a nanographene intermolecular tunnel junction achieved via scanning tunnelling microscope tip functionalization with hexa-peri-hexabenzocoronene (HBC) molecules. This leads to an offset in the alignment between the energy levels of the tip and the molecular assembly, resulting in the imaging of a variety of distinct charge density patterns in the HBC assembly, not attainable using a bare metallic tip. Different tunnelling channels can be selected by the application of an electric field in the tunnelling junction, which changes the condition of the HBC on the tip. Density functional theory-based calculations relate the imaged HBC patterns to the calculated molecular orbitals at certain energy levels. These patterns bear a close resemblance to the π-orbital states of the HBC molecule calculated at the relevant energy levels, mainly below the Fermi energy of HBC. This correlation demonstrates the ability of an HBC functionalized tip as regards accessing an energy range that is restricted to the usual operating bias range around the Fermi energy with a normal metallic tip at room temperature. Apart from relating to molecular orbitals, some patterns could also be described in association with the Clar aromatic sextet formula. Our observations may help pave the way towards the possibility of controlling charge transport between organic interfaces.

Nearest neighbour decoding with pilot-assisted channel estimation for fading multiple-access channels

This paper studies a noncoherent multiple-input multiple-output (MIMO) fading multiple-access channel (MAC). The rate region that is achievable with nearest neighbour decoding and pilot-assisted channel estimation is analysed and the corresponding pre-log region, defined as the limiting ratio of the rate region to the logarithm of the signal-to-noise ratio (SNR) as the SNR tends to infinity, is determined. © 2011 IEEE.

Effects of ginkgo biloba extract on cation currents in rat ventricular myocytes

Ginkgo biloba extract (GBE), a valuable natural product for cerebral and cardiovascular diseases, is mainly composed of two classes of constituents: terpene lactones (e.g., ginkgolide A and B, bilobalide) and flavone glycosides (e.g., quercetin and kaempferol). Its electrophysiological action in heart is yet unclear. In the present study, using whole-cell patch clamp technique, we investigated electrophysiological effects of GBE on cation channel currents in ventricular myocytes isolated from rat hearts. We found that GBE 0.01-0.1% inhibited significantly the sodium current (I-Na), L-type calcium current (I-Ca) and transient outward potassium current (IKto) in a concentration-dependent manner. Surprisingly, its main ingredients, ginkgolide A (GB A), ginkgolide B (GB B) and bilobalide (GB BA) at 0.1 mM did not exhibit any significant effect on these cation channel currents. These results suggested that GBE is a potent non-selective cation channel modulator in cardiaomyocytes. Other constituents (rather than GB A, GB B and GB BA) might be responsible for the observed inhibitory effects of GBE on cation channels. (C) 2004 Elsevier Inc. All rights reserved.

In vitro anti-HIV and -HSV activity and safety of sodium rutin sulfate as a microbicide candidate

Sodium rutin sulfate (SRS) is a sulfated rutin modified from the natural flavonol glycoside rutin. Here, we investigated its in vitro anti-HIV and -HSV activities and its cytotoxic profile. Fifty percent inhibitory concentration (IC50) values of SRS against HIV-1 X4 virus IIIB, HIV-1 R5 isolates Ada-M and Ba-L were 2.3 +/- 0.2, 4.5 +/- 2.0 and 8.5 +/- 3.8 mu M with a selectivity index (SI) of 563, 575 and 329, respectively. Its IC50 against primary R5 HIV-1 isolate from Yunnan province in China was 13.1 +/- 5.5 mu M, with a Sl of 197. In contrast, unsulfated rutin had no activity against any of the HIV-1 isolates tested. Further study indicated that SRS blocked viral entry and virus-cell fusion likely through interacting with the HIV- I envelope glycoprotein. SRS also demonstrated some activity against human herpes simplex virus (HSV) with an IC50 of 88.3 +/- 0.1 mu M and a Sl of 30. The 50% cytotoxicity concentration (CC50) of SRS was >3.0 mM, as determined in human genital ME 180, HeLa and primary human foreskin fibroblast cells. Minimum inhibitory concentration of SRS for vaginal lactobacilli was >3.0 mM. These results collectively indicate that SRS represents a novel candidate for anti-HIV-1/HSV microbicide development. (C) 2007 Elsevier B.V. All rights reserved.