Identificação dos princípios ativos antiulcerogênicos das folhas de Casearia sylvestris: contribuição para o desenvolvimento de um fitoterápico

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Caracterização quali e quantitativa de metabólitos secundários em extratos vegetais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Propriedades químicas e físicas do material particulado atmosférico e seus efeitos no crescimento de partículas

Pós-graduação em Química - IQ

Peptídeos derivados da toxina ParE: síntese, estrutura e estudos de inibição de DNA topoisomerases

Pós-graduação em Biotecnologia - IQ

Immunodetection of cells with a CD44+/CD24-phenotype in canine mammary neoplasms

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Conservative Approach: Using Decompression Procedure for Management of a Large Unicystic Ameloblastoma of the Mandible

Ameloblastoma is a relatively uncommon benign odontogenic tumor, which is locally aggressive and has a high tendency to recur, despite its benign histopathologic features. This pathology can be classified into 4 groups: unicystic, solid or multicystic, peripheral, and malignant. There are 3 variants of unicystic ameloblastoma, as luminal, intraluminal, and mural. Therefore, in mural ameloblastoma, the fibrous wall of the cyst is infiltrated with tumor nodules, and for this reason it is considered the most aggressive variant of unicystic ameloblastomas. Various treatment techniques for ameloblastomas have been proposed, which include decompression, enucleation/curettage, sclerotizing solution, cryosurgery, marginal resection, and aggressive resection. Literature shows treatment of this lesion continues to be a subject of intense interest and some controversy. Thus, the authors aimed to describe a case of a mural unicystic ameloblastoma of follicular subtype in a 19-year-old subject who was successfully treated using conservative approaches, as decompression. The patient has been followed up for 3 years, and has remained clinically and radiographically disease-free.

Estudo imuno-histoquímico da co-expressão de citoqueratinas, vimentina e actina em neoplasias de glândula salivar

Human salivary gland tumors originated from intercalated ducts present a broad range of histologic and cytologic patterns, mainly due to the presence of myoepithelial cells. The aim of this study is to verify the differentiation grade of neoplastic cells and a possible relation between myoepithelial cell differentiation and the presence of luminal secretory contents. The expression of vimentin and cytokeratin (CK) intermediate filaments, actin myofilament and epithelial membrane antigen (EMA) was investigated by double labeling immunocytochemical technique, in thirty salivary gland neoplasms: 5 pleomorphic adenomas, 5 myoepitheliomas, 3 basal cell adenomas, 7 adenoid cystic carcinomas (ACC) and 10 polimorphous low grade adenocarcinomas (PLGA). Tumors with intercalated duct differentiation (pleomorphic adenomas, basal cell adenomas and ACC) express CKs 7, 8, 18 and 19 in the luminal cells and coexpress eventually CK14 with these CKs. Some luminal cells stained with anti-EMA antibody, mainly where a secretory content in the lumen was observed. Outer ductal cells and other myoepithelial-like cells express vimentin, sometimes coexpressing actin and/or CK14 with vimentin. Plasmacytoid cells in myoepitheliomas and pleomorphic adenomas express vimentin and rarely CKs 7, 8, 18 and 19, sometimes coexpressing these CKs with CK14 but they are negative for the remaining antigens. Tumors without intercalated duct differentiation (solid basal cell adenoma and PLGA) express vimentin and CKs 7, 8, 14 and 18, sometimes coexpressing CKs 8 and 18 with CK14. In conclusion, in tumors with intercalated duct differentiation, myoepithelial cells express vimentin and sometimes coexpress actin and/or CK14 with vimentin, never coexpressing other CKs with vimentin. CK14 and actin are independently expressed by myoepithelial cells, so their expression is probably induced by different stimulus. However, the secretory function of luminal cells, visualized by EMA staining, ....

Cooperviva: análise das possibilidades e limites do trabalho dos catadores de resíduos recicláveis de Rio Claro (SP)

The issue of waste, historically, has been getting worse as a result of the world´s population increase and in order to meet their needs the consumption of natural resources and the generation has been constantly increasing, especially in large urban centers. The people involved in the collection of recyclable solid waste, called from now on as collectors, are responsible for about 90% of the waste sent to recycling industry in Brazil. Apart from having a role in the economy, the collectors have specific knowledge and skills necessary to identify, collect, sort and sell these wastes. This study aims to examine the possibilities and limits of COOPERVIVA – Cooperativa de Trabalho dos Catadores de Material Reaproveitável de Rio Claro - as an instrument of the municipal solid waste management system, and its experience as a recyclable waste collectors cooperative. The methodological procedures of this study rely on the literature research on the experiences of cooperative and the management of municipal solid waste. In addition to this review of the literature, fortnightly visits to the cooperative were held in order to experience their daily activities, and testimonies of the cooperative members were collected as well as documents and reports produced by COOPERVIVA. The results of this analysis are considerations of the limitations of the cooperative and recommendations aimed at maximizing their potential as a tool of the domestic solid waste management system of and as a recyclable waste collectors cooperative. Among other things it can be concluded that the urban selective collection of waste challenges the community to the reinvention of life and the relationships each individual with the planet.

A geração de resíduos sólidos urbanos e sua influência na gestão urbano-ambiental municipal de Sorocaba/SP

In Brazil, rates of urban solid waste generation increase proportionally more than the annual growing of the population. The government, from its federal authority to the municipal spheres, faces increasing challenges for Urban and Environmental management regarding to solid waste in general and, in particular, municipal solid waste. Financial problems added to public health problems that worsen with the serious environmental impacts generated every year. In August 2010 Brazil the PNRS (National Policy on Solid Waste) was approved and sanctioned in which represented a true watershed in the country as a legal framework extremely ambitious and visionary. This work presents the successes and challenges that the municipality of Sorocaba/SP is facing after more than three years of PNRS sanctioned to conduct an Urban and Environmental management suited to the new legal bases towards the subject of solid waste. The results show a large gap between the content proposed by the law and the technical, financial and temporal reality to practical implementation by the municipal government. New legislation and federal incentives, consortiums among the cities, flexible deadlines and environmental education at all levels are certainly some proposals to catalyze processes that aim to solve and overcome the challenges of solid waste

Síntese e caracterização do complexo de lantânio (III) e indometacina no estado sólido

In this study, the drug indomethacin, a non-steroidal anti-inflammatory indoleacetic acid derivative and the complex of indomethacin and lanthanum (III) in solid form were synthesized and characterized by Thermogravimetry (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC) and powder X-ray diffractometry (XRD), infrared vibrational spectroscopy by diffuse reflectance (FTIR) and complexometric titration with EDTA. With the TG curves it was possible to determine the stoichiometry of the complex as La(Ind)3·3.5H2O where Ind is the drug indomethacin. The result of thermal analyzes provided information on the thermal stability, enthalpy of dehydration and thermal behavior of the compounds. The infrared spectrum and with the aid of theoretical calculations suggests that the indomethacin is coordinated by the carboxylate group in the bidentate mode

Gestão ambiental na produção avícola: estudo de caso

The present study addresses the application of the Environmental Management System in chicken production on a farm owned by the Nardo brothers, located in the city of Guapiaçu/SP (Brazil). The objectives were to analyze the process of solid waste generation, destination and forms of treatment in this activity; propose measures to reduce the use of potable water for non-potable ends; determine other measures identified through a questionnaire drafted by Embrapa (Brazilian Agriculture Research Cooperative) and administered to employees who have direct involvement in the productive process; pay visits to chicken production enterprises; and employ the guidelines proposed by the National Fowl-Breeding Sanitation Program that guide the application of the Environmental Management System. Adequate management of waste was put into effect through the construction of a compost shed capable of meeting current production demands, in which all procedures were accompanied – from civil construction to the commercialization process of the organic fertilizer generated. The rain-gathering system for the roofs of the pens proposed to reduce the consumption of potable ground water destined for washing the floors of the pens was completely dimensioned and budgeted, allowing the motivated owners to place the project into effect in a timely fashion. Other simpler but no less important proposals were also made in order to bring the production up to the required quality standards.

Estudo de compósitos de madeira com reforço em bambu para utilização em mobiliário

Bamboo has been studied because of its peculiar mechanical properties and numerous possibilities of use, besides being a fast-growing grass and short cutting cycle. This study aimed to analyze the mechanical characteristics of wood-bamboo composite material, where the samples were developed from the combination of layers of bamboo as a structural reinforcement in solid pieces of pine and EGP panel parts. The species of wood used was Pinus taeda, and the bamboo species Guadua angustifolia and Dendrocalamus giganteus. All work was conducted at the Universidade Estadual Paulista - UNESP in the laboratory of Physical and Mechanical Properties of Wood. Tests including the density and tension parallel to grain of the bamboo species used and the static bending of composites in order to use this in the furniture industry. For the tests have been used as a basis the requirements of the normative document NBR 7190/97. The values obtained in the tests showed a significant increase in strength and stiffness compared to unreinforced parts, where there was an increase in MOE and MOR in static bending in all specimens used in evidence. The results showed the possibility of reducing sections in furniture components and the possibility of improving the mechanical properties of parts with defects found in wood of Pinus Itapeva region of São Paulo

Determination of anticonvulsants in human plasma using SPME in a heated interface coupled online to liquid chromatography (SPME-LC)

A simple and sensitive method using solid phase microextraction (SPME) and liquid chromatography (LC) with heated online desorption (SPME-LC) was developed and validated to analyze anticonvulsants (AEDs) in human plasma samples. A heated lab-made interface chamber was used in the desorption procedure, which allowed the transference of the whole extracted sample. The SPME conditions were optimized by applying an experimental design. Important factors are discussed such as fiber coating types, pH, extraction time and desorption conditions. The drugs were analyzed by LC, using a C18 column (150 mm 4.6 mm 5 mm); and 50 mmol L1 , pH ¼ 5.50 ammonium acetate buffer : acetonitrile : methanol (55 : 22 : 23 v/v) as the mobile phase with a flow rate of 0.8 mL min1 . The suggested method presented precision (intra-assay and inter-assay), linearity and limit of quantification (LOQ) all adequate for the therapeutic drug monitoring (TDM) of AEDs in plasma.

Analysis of fluoxetine and norfluoxetine in human plasma by HPLC-UV using a high purity C18 silica-based SPE sorbent

This paper reports on the development and validation of a simple and sensitive method that uses solid phase extraction (SPE) and liquid chromatography with ultraviolet detection to analyze fluoxetine (FLX) and norfluoxetine (NFLX) in human plasma samples. A lab-made C18 SPE phase was synthesized by using a sol–gel process employing a low-cost silica precursor. This sorbent was fully characterized by nuclear magnetic resonance (NMR), Fourier-transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM) to check the particles' shape, size and C18 functionalization. The lab-made C18 silica was used in the sample preparation step of human plasma by the SPE-HPLC-UV method. The method was validated in the 15 to 500 ng mL 1 range for both FLX and NFLX using a matrix matched curve. Detection limits of 4.3 and 4.2 ng mL 1 were obtained for FLX and NFLX, respectively. The repeatability and intermediary precision achieved varied from 7.6 to 15.0% and the accuracy ranged from 14.9 to 9.1%. The synthesized C18 sorbent was compared to commercial C18 sorbents. The average recoveries were similar (85–105%), however the lab-made C18 silica showed fewer interfering peaks in the chromatogram. After development and validation, the method using the lab-made C18 SPE was applied to plasma samples of patients under FLX treatment (n ¼ 6). The concentrations of FLX and NFLX found in the samples varied from 46.8–215.5 and 48.0–189.9 ng mL 1 , respectively.

Síntese, estrutura e atividade de peptídeos derivados de ParD, o antídoto do módulo toxina-antitoxina ParDE

Pós-graduação em Biotecnologia - IQ