Advantages of digital holographic microscopy for real-time full field absolute phase imaging

Different interferometric techniques were developed last decade to obtain full field, quantitative, and absolute phase imaging, such as phase-shifting, Fourier phase microscopy, Hilbert phase microscopy or digital holographic microscopy (DHM). Although, these techniques are very similar, DHM combines several advantages. In contrast, to phase shifting, DHM is indeed capable of single-shot hologram recording allowing a real-time absolute phase imaging. On the other hand, unlike to Fourier phase or Hilbert phase microscopy, DHM does not require to record in focus images of the specimen on the digital detector (CCD or CMOS camera), because a numerical focalization adjustment can be performed by a numerical wavefront propagation. Consequently, the depth of view of high NA microscope objectives is numerically extended. For example, two different biological cells, floating at different depths in a liquid, can be focalized numerically from the same digital hologram. Moreover, the numerical propagation associated to digital optics and automatic fitting procedures, permits vibrations insensitive full- field phase imaging and the complete compensation for a priori any image distortion or/and phase aberrations introduced for example by imperfections of holders or perfusion chamber. Examples of real-time full-field phase images of biological cells have been demonstrated. ©2008 COPYRIGHT SPIE

Reverse transcription-polymerase chain reaction construction of plasmid-based, full-length cDNA libraries from Leishmania infantum for in vitro expression screening

We describe a streamlined reverse transcription-polymerase chain reaction methodology for constructing full-length cDNA libraries of trypanosomatids on the basis of conserved sequences located at the 5' and 3'ends of trans-spliced mRNAs. The amplified cDNA corresponded to full-length messengers and was amenable to in vitro expression. Fractionated libraries could be rapidly constructed in a plasmid vector by the TA cloning method (Invitrogen). We believe this is useful when there are concerns over the use of restriction enzymes and phage technology as well as in cases where expression of proteins in their native conformation is desired.

Memòria metodològica de treball plantejada per a l'elaboració del projecte Alfabetització informacional : disseny d'estratègies, de serveis i recursos per a la fidelització d'usuaris de la biblioteca universitària

El treball se centra en l'anàlisi de les causes de la baixa participació en les sessions de formació d'usuaris per part dels estudiants, professors i investigadors universitaris de la Biblioteca de Física i Química de la Universitat de Barcelona. Es remarca la importància d'aquesta formació en la cerca, selecció i difusió de la informació així com la inclusió d'un programa d'alfabetització informacional. Es presenten possibles solucions i propostes per tal d'augmentar aquesta assistència (major difusió, publicitat...), amortitzar els recursos i serveis que preparen els bibliotecaris i en definitiva fidelitzar aquests usuaris.

Red blood cell structure and dynamics explored with digital holographic microspcopy

Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. Thanks to a decoupling procedure cell thickness and intracellular refractive index can be measured separately. Consequently, Mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), two highly relevant clinical parameters, have been measured non-invasively at a single cell level. The DHM nanometric axial and microsecond temporal sensitivities have permitted to measure the red blood cell membrane fluctuations (CMF) on the whole cell surface. ©2009 COPYRIGHT SPIE--The International Society for Optical Engineering.

ARCHITECTING USER-CENTRIC PRIVACY-AS-A-SET-OF-SERVICES: DIGITAL IDENTITY RELATED PRIVACY FRAMEWORK

AbstractDigitalization gives to the Internet the power by allowing several virtual representations of reality, including that of identity. We leave an increasingly digital footprint in cyberspace and this situation puts our identity at high risks. Privacy is a right and fundamental social value that could play a key role as a medium to secure digital identities. Identity functionality is increasingly delivered as sets of services, rather than monolithic applications. So, an identity layer in which identity and privacy management services are loosely coupled, publicly hosted and available to on-demand calls could be more realistic and an acceptable situation. Identity and privacy should be interoperable and distributed through the adoption of service-orientation and implementation based on open standards (technical interoperability). Ihe objective of this project is to provide a way to implement interoperable user-centric digital identity-related privacy to respond to the need of distributed nature of federated identity systems. It is recognized that technical initiatives, emerging standards and protocols are not enough to guarantee resolution for the concerns surrounding a multi-facets and complex issue of identity and privacy. For this reason they should be apprehended within a global perspective through an integrated and a multidisciplinary approach. The approach dictates that privacy law, policies, regulations and technologies are to be crafted together from the start, rather than attaching it to digital identity after the fact. Thus, we draw Digital Identity-Related Privacy (DigldeRP) requirements from global, domestic and business-specific privacy policies. The requirements take shape of business interoperability. We suggest a layered implementation framework (DigldeRP framework) in accordance to model-driven architecture (MDA) approach that would help organizations' security team to turn business interoperability into technical interoperability in the form of a set of services that could accommodate Service-Oriented Architecture (SOA): Privacy-as-a-set-of- services (PaaSS) system. DigldeRP Framework will serve as a basis for vital understanding between business management and technical managers on digital identity related privacy initiatives. The layered DigldeRP framework presents five practical layers as an ordered sequence as a basis of DigldeRP project roadmap, however, in practice, there is an iterative process to assure that each layer supports effectively and enforces requirements of the adjacent ones. Each layer is composed by a set of blocks, which determine a roadmap that security team could follow to successfully implement PaaSS. Several blocks' descriptions are based on OMG SoaML modeling language and BPMN processes description. We identified, designed and implemented seven services that form PaaSS and described their consumption. PaaSS Java QEE project), WSDL, and XSD codes are given and explained.

Real-time dual-wavelength digital holographic microscopy for extended measurement range with enhanced axial resolution

We report on advanced dual-wavelength digital holographic microscopy (DHM) methods, enabling single-acquisition real-time micron-range measurements while maintaining single-wavelength interferometric resolution in the nanometer regime. In top of the unique real-time capability of our technique, it is shown that axial resolution can be further increased compared to single-wavelength operation thanks to the uncorrelated nature of both recorded wavefronts. It is experimentally demonstrated that DHM topographic investigation within 3 decades measurement range can be achieved with our arrangement, opening new applications possibilities for this interferometric technique. ©2008 COPYRIGHT SPIE

Bases de dades multimedia

Aquest treball vol fer conèixer d'una manera més o menys acurada el concepte de base de dades multimèdia i tot el que hi està relacionat. Amb aquesta finalitat, el treball s'ha estructurat de manera que el lector conegui els conceptes fonamentals de les bases de dades en general i de les multimèdia en concret, com també les tecnologies disponibles per a gestionar-les, saber com funcionen, què ofereixen, etc.

Recent Progress and Perspectives in Digital Holographic Microscopy

Digital Holographic Microscopy (DHM), is a new imaging technique allowing to provide quantitative phase images with a high accuracy and stability making possible to explore a large variety of relevant processes, occurring on the p.s to day time scale, in the fields including material research as well as cell biology. As a non invasive and real time imaging technique, DHM is particularly well suited for high throughput screening

Informatización de una biblioteca mediante una BD multimedia

El projecte té com objectiu desenvolupar una aplicació informàtica per a una biblioteca. El principal handicap que tindrà aquesta aplicació és que algunes de les dades que s'hi volen desar són de diversos formats: imatges, sons, dibuixos, pel·lícules de DVD, arxius doc, arxius application/pdf, etc.

Bases de datos Multimedia

Aquest treball mira d'aproximar-se a la situació actual de les bases de dades multimèdia. Per a fer-ho, s'ha optat per un enfocament múltiple que té en compte diferents aspectes del producte multimèdia: els aspectes econòmics, és a dir, la situació del mercat (nombre de bases de dades, volum de negoci, creixement, etc.), els aspectes tècnics, és a dir, l'eina emprada, la seva evolució, les seves limitacions i problemes, i les seves aplicacions i serveis en diferents àrees (amb un interès especial en el camp de la documentació i la formació i la recerca).

Anàlisi i Disseny d'una aplicación pel Vot Digital

El treball consisteix en un sistema de votació basat en l'anàlisi i disseny basat en les noves tecnologies. L'aplicació es digués VotoDigital, l'esmentat sistema dugués a terme i gestionés el procés de votació.

Digital Strategy for Schools 2015 - 2020

Among the key points of the Digital Strategy are: Dedicated multi-annual funding to schools to invest in technology Build on the successful roll-out of high-speed broadband to every second-level school by investing in high-speed wifi networks in every school Integration of digital skills in the curriculum and in assessment Develop opportunities for students to take an in-depth ICT course at Leaving Cert, as well as embedding digital skills within other subjects Promotion of the use of e-portfolios at primary and post-primary level Provide enhanced digital content to schools, including working with cultural institutions, sporting bodies and other to expand this range of resources Embed ICT skills as part of initial teacher education and ongoing training for teachers

Digital Strategy for Schools 2015-2020 Report on the Consultation with Young People

The Department of Education and Skills is developing a new Digital Strategy for Schools to be completed during 2015. The Digital Strategy for Schools will set out how Department of Education and Skills’ priorities can be delivered and facilitated by the use of technology in education. The development of the Strategy is of critical importance to Ireland if it is to realise the potential of ICT in schools and prepare our young people to live, learn and work in the 21st century. The Department of Education and Skills has partnered with the Department of Children and Youth Affairs (DCYA) to seek the views of young people through a structured consultation process informed by DCYA expertise in consulting with young people.

Bases de dades multimedia

La part del treball de recerca inclou un estudi que recull l'evolució de les bases de dades des del seu començament fins a l'actualitat, incloent-hi una previsió del futur de les bases de dades; finalment, com a aprofundiment, s'hi estudien les bases de dades multimèdia. D'altra banda, la part pràctica mira d'aplicar tots el coneixements adquirits durant l'estudi de recerca i durant les diferents assignatures de la carrera, i intenta obtenir una aplicació que es pugui fer servir en el món real.