A modular LHC built on the DNA three-way junction

Over the past years, in numerous studies the DNA double helix serves as a scaffold for the controlled arrangement of functional molecules, including a wide range of different chromophores. Other nucleic acid structures like the DNA three-way junction have been exploited for this purpose as well. Recently, the successful development of DNA-based light-harvesting antenna systems have been reported. Herein, we describe the use of the DNA three-way junction (3WJ) as a versatile scaffold for the modular construction of an artificial light harvesting complex (LHC). The LHC is based on a modular construction in which a phenanthrene antenna is located in one of the three stems and the acceptor is brought into proximity of the antenna through the annealing of the third strand. Phenanthrene excitation (320 nm) is followed by energy transfer to pyrene (resulting in exciplex emission), perylenediimide (quencher) or a cyanine dye (cyanine fluorescence).

Real-Time Localization Using Software Defined Radio

Service providers make use of cost-effective wireless solutions to identify, localize, and possibly track users using their carried MDs to support added services, such as geo-advertisement, security, and management. Indoor and outdoor hotspot areas play a significant role for such services. However, GPS does not work in many of these areas. To solve this problem, service providers leverage available indoor radio technologies, such as WiFi, GSM, and LTE, to identify and localize users. We focus our research on passive services provided by third parties, which are responsible for (i) data acquisition and (ii) processing, and network-based services, where (i) and (ii) are done inside the serving network. For better understanding of parameters that affect indoor localization, we investigate several factors that affect indoor signal propagation for both Bluetooth and WiFi technologies. For GSM-based passive services, we developed first a data acquisition module: a GSM receiver that can overhear GSM uplink messages transmitted by MDs while being invisible. A set of optimizations were made for the receiver components to support wideband capturing of the GSM spectrum while operating in real-time. Processing the wide-spectrum of the GSM is possible using a proposed distributed processing approach over an IP network. Then, to overcome the lack of information about tracked devices’ radio settings, we developed two novel localization algorithms that rely on proximity-based solutions to estimate in real environments devices’ locations. Given the challenging indoor environment on radio signals, such as NLOS reception and multipath propagation, we developed an original algorithm to detect and remove contaminated radio signals before being fed to the localization algorithm. To improve the localization algorithm, we extended our work with a hybrid based approach that uses both WiFi and GSM interfaces to localize users. For network-based services, we used a software implementation of a LTE base station to develop our algorithms, which characterize the indoor environment before applying the localization algorithm. Experiments were conducted without any special hardware, any prior knowledge of the indoor layout or any offline calibration of the system.

Real-time localization using software defined radio

Service providers make use of cost-effective wireless solutions to identify, localize, and possibly track users using their carried MDs to support added services, such as geo-advertisement, security, and management. Indoor and outdoor hotspot areas play a significant role for such services. However, GPS does not work in many of these areas. To solve this problem, service providers leverage available indoor radio technologies, such as WiFi, GSM, and LTE, to identify and localize users. We focus our research on passive services provided by third parties, which are responsible for (i) data acquisition and (ii) processing, and network-based services, where (i) and (ii) are done inside the serving network. For better understanding of parameters that affect indoor localization, we investigate several factors that affect indoor signal propagation for both Bluetooth and WiFi technologies. For GSM-based passive services, we developed first a data acquisition module: a GSM receiver that can overhear GSM uplink messages transmitted by MDs while being invisible. A set of optimizations were made for the receiver components to support wideband capturing of the GSM spectrum while operating in real-time. Processing the wide-spectrum of the GSM is possible using a proposed distributed processing approach over an IP network. Then, to overcome the lack of information about tracked devices’ radio settings, we developed two novel localization algorithms that rely on proximity-based solutions to estimate in real environments devices’ locations. Given the challenging indoor environment on radio signals, such as NLOS reception and multipath propagation, we developed an original algorithm to detect and remove contaminated radio signals before being fed to the localization algorithm. To improve the localization algorithm, we extended our work with a hybrid based approach that uses both WiFi and GSM interfaces to localize users. For network-based services, we used a software implementation of a LTE base station to develop our algorithms, which characterize the indoor environment before applying the localization algorithm. Experiments were conducted without any special hardware, any prior knowledge of the indoor layout or any offline calibration of the system.

From supramolecular sheets to fibers and back: establishing the critical parameters that govern the morphology of pyrene-based self-assembled materials

The precise arraying of functional entities in morphologically well-defined shapes remains one of the key challenges in the processing of organic molecules1. Among various π-conjugated species, pyrene exhibits a set of unique properties, which make it an attractive compound for the utilization in materials science2. In this contribution we report on properties of self-assembled structures prepared from amphiphilic pyrene trimers (Py3) consisting of phosphodiester-linked pyrenes. Depending on the geometry of a pyrene core substitution (1.6-, 1.8-, or 2.7- type, see Scheme), the thermally-controlled self-assembly allows the preparation of supramolecular architectures of different morphologies in a bottom-up approach: two-dimensional (2D) nanosheets3 are formed in case of 1.6- and 2.7-substitution4 whereas one-dimensional (1D) fibers are built from 1.8- substituted isomers. The morphologies of the assemblies are established by AFM and TEM, and the results are further correlated with spectroscopic and scattering data. Two-dimensional assemblies consist of an inner layer of hydrophobic pyrenes, sandwiched between a net of phosphates. Due to the repulsion of the negative charges, the 2D assemblies exist mostly as free-standing sheets. An internal alignment of pyrenes leads to strong exciton coupling with an unprecedented observation (simultaneous development of J- and H-bands from two different electronic transitions). Despite the similarity in spectroscopic properties, the structural parameters of the 2D aggregates drastically depend on the preparation procedure. Under certain conditions extra-large sheets (thickness of 2 nm, aspect ratio area/thickness ~107) in aqueous solution are formed4B. Finally, one-dimensional assemblies are formed as micrometer-long and nanometer-thick fibers. Both, planar and linear structures are intriguing objects for the creation of conductive nanowires that may find interest for applications in supramolecular electronics.

Exact Unification and Admissibility

A new hierarchy of "exact" unification types is introduced, motivated by the study of admissible rules for equational classes and non-classical logics. In this setting, unifiers of identities in an equational class are preordered, not by instantiation, but rather by inclusion over the corresponding sets of unified identities. Minimal complete sets of unifiers under this new preordering always have a smaller or equal cardinality than those provided by the standard instantiation preordering, and in significant cases a dramatic reduction may be observed. In particular, the classes of distributive lattices, idempotent semigroups, and MV-algebras, which all have nullary unification type, have unitary or finitary exact type. These results are obtained via an algebraic interpretation of exact unification, inspired by Ghilardi's algebraic approach to equational unification.

Linking soil properties and pre-Columbian agricultural strategies in the Bolivian lowlands: The case of raised fields in Exaltación

This paper aims to further our understanding of pre-Columbian agricultural systems in the Llanos de Moxos, Bolivia. Three different types of raised fields co-existing in the same site near the community of Exaltación, in north-western Beni, were studied. The morphology, texture and geochemistry of the soils of these fields and the surrounding area were analysed. Differences in field design have often been associated with the diversity of cultural practices. Our results suggest that in the study area differences in field shape, height and layout are primarily the result of an adaptation to the local edaphology. By using the technology of raised fields, pre-Columbian people were able to drain and cultivate soils with very different characteristics, making the land suitable for agriculture and possibly different crops. This study also shows that some fields in the Llanos de Moxos were built to prolong the presence of water, allowing an additional cultivation period in the dry season and/or in times of drought. Nevertheless, the nature of the highly weathered soils suggests that raised fields were not able to support large populations and their management required long fallow periods.

Endemic tuberculosis, the homeless, and public transportation: A merging of geographical information systems surveillance and the Houston Tuberculosis Initiative Surveillance project

To reach the goals established by the Institute of Medicine (IOM) and the Centers for Disease Control's (CDC) STOP TB USA, measures must be taken to curtail a future peak in Tuberculosis (TB) incidence and speed the currently stagnant rate of TB elimination. Both efforts will require, at minimum, the consideration and understanding of the third dimension of TB transmission: the location-based spread of an airborne pathogen among persons known and unknown to each other. This consideration will require an elucidation of the areas within the U.S. that have endemic TB. The Houston Tuberculosis Initiative (HTI) was a population-based active surveillance of confirmed Houston/Harris County TB cases from 1995–2004. Strengths in this dataset include the molecular characterization of laboratory confirmed cases, the collection of geographic locations (including home addresses) frequented by cases, and the HTI time period that parallels a decline in TB incidence in the United States (U.S.). The HTI dataset was used in this secondary data analysis to implement a GIS analysis of TB cases, the locations frequented by cases, and their association with risk factors associated with TB transmission. ^ This study reports, for the first time, the incidence of TB among the homeless in Houston, Texas. The homeless are an at-risk population for TB disease, yet they are also a population whose TB incidence has been unknown and unreported due to their non-enumeration. The first section of this dissertation identifies local areas in Houston with endemic TB disease. Many Houston TB cases who reported living in these endemic areas also share the TB risk factor of current or recent homelessness. Merging the 2004–2005 Houston enumeration of the homeless with historical HTI surveillance data of TB cases in Houston enabled this first-time report of TB risk among the homeless in Houston. The homeless were more likely to be US-born, belong to a genotypic cluster, and belong to a cluster of a larger size. The calculated average incidence among homeless persons was 411/100,000, compared to 9.5/100,000 among housed. These alarming rates are not driven by a co-infection but by social determinants. The unsheltered persons were hospitalized more days and required more follow-up time by staff than those who reported a steady housing situation. The homeless are a specific example of the increased targeting of prevention dollars that could occur if TB rates were reported for specific areas with known health disparities rather than as a generalized rate normalized over a diverse population. ^ It has been estimated that 27% of Houstonians use public transportation. The city layout allows bus routes to run like veins connecting even the most diverse of populations within the metropolitan area. Secondary data analysis of frequent bus use (defined as riding a route weekly) among TB cases was assessed for its relationship with known TB risk factors. The spatial distribution of genotypic clusters associated with bus use was assessed, along with the reported routes and epidemiologic-links among cases belonging to the identified clusters. ^ TB cases who reported frequent bus use were more likely to have demographic and social risk factors associated with poverty, immune suppression and health disparities. An equal proportion of bus riders and non-bus riders were cultured for Mycobacterium tuberculosis, yet 75% of bus riders were genotypically clustered, indicating recent transmission, compared to 56% of non-bus riders (OR=2.4, 95%CI(2.0, 2.8), p<0.001). Bus riders had a mean cluster size of 50.14 vs. 28.9 (p<0.001). Second order spatial analysis of clustered fingerprint 2 (n=122), a Beijing family cluster, revealed geographic clustering among cases based on their report of bus use. Univariate and multivariate analysis of routes reported by cases belonging to these clusters found that 10 of the 14 clusters were associated with use. Individual Metro routes, including one route servicing the local hospitals, were found to be risk factors for belonging to a cluster shown to be endemic in Houston. The routes themselves geographically connect the census tracts previously identified as having endemic TB. 78% (15/23) of Houston Metro routes investigated had one or more print groups reporting frequent use for every HTI study year. We present data on three specific but clonally related print groups and show that bus-use is clustered in time by route and is the only known link between cases in one of the three prints: print 22. (Abstract shortened by UMI.)^

UNDERSTANDING INTERRUPTIONS IN HEALTHCARE: DEVELOPING A MODEL

Developing a Model Interruption is a known human factor that contributes to errors and catastrophic events in healthcare as well as other high-risk industries. The landmark Institute of Medicine (IOM) report, To Err is Human, brought attention to the significance of preventable errors in medicine and suggested that interruptions could be a contributing factor. Previous studies of interruptions in healthcare did not offer a conceptual model by which to study interruptions. As a result of the serious consequences of interruptions investigated in other high-risk industries, there is a need to develop a model to describe, understand, explain, and predict interruptions and their consequences in healthcare. Therefore, the purpose of this study was to develop a model grounded in the literature and to use the model to describe and explain interruptions in healthcare. Specifically, this model would be used to describe and explain interruptions occurring in a Level One Trauma Center. A trauma center was chosen because this environment is characterized as intense, unpredictable, and interrupt-driven. The first step in developing the model began with a review of the literature which revealed that the concept interruption did not have a consistent definition in either the healthcare or non-healthcare literature. Walker and Avant’s method of concept analysis was used to clarify and define the concept. The analysis led to the identification of five defining attributes which include (1) a human experience, (2) an intrusion of a secondary, unplanned, and unexpected task, (3) discontinuity, (4) externally or internally initiated, and (5) situated within a context. However, before an interruption could commence, five conditions known as antecedents must occur. For an interruption to take place (1) an intent to interrupt is formed by the initiator, (2) a physical signal must pass a threshold test of detection by the recipient, (3) the sensory system of the recipient is stimulated to respond to the initiator, (4) an interruption task is presented to recipient, and (5) the interruption task is either accepted or rejected by v the recipient. An interruption was determined to be quantifiable by (1) the frequency of occurrence of an interruption, (2) the number of times the primary task has been suspended to perform an interrupting task, (3) the length of time the primary task has been suspended, and (4) the frequency of returning to the primary task or not returning to the primary task. As a result of the concept analysis, a definition of an interruption was derived from the literature. An interruption is defined as a break in the performance of a human activity initiated internal or external to the recipient and occurring within the context of a setting or location. This break results in the suspension of the initial task by initiating the performance of an unplanned task with the assumption that the initial task will be resumed. The definition is inclusive of all the defining attributes of an interruption. This is a standard definition that can be used by the healthcare industry. From the definition, a visual model of an interruption was developed. The model was used to describe and explain the interruptions recorded for an instrumental case study of physicians and registered nurses (RNs) working in a Level One Trauma Center. Five physicians were observed for a total of 29 hours, 31 minutes. Eight registered nurses were observed for a total of 40 hours 9 minutes. Observations were made on either the 0700–1500 or the 1500-2300 shift using the shadowing technique. Observations were recorded in the field note format. The field notes were analyzed by a hybrid method of categorizing activities and interruptions. The method was developed by using both a deductive a priori classification framework and by the inductive process utilizing line-byline coding and constant comparison as stated in Grounded Theory. The following categories were identified as relative to this study: Intended Recipient - the person to be interrupted Unintended Recipient - not the intended recipient of an interruption; i.e., receiving a phone call that was incorrectly dialed Indirect Recipient – the incidental recipient of an interruption; i.e., talking with another, thereby suspending the original activity Recipient Blocked – the intended recipient does not accept the interruption Recipient Delayed – the intended recipient postpones an interruption Self-interruption – a person, independent of another person, suspends one activity to perform another; i.e., while walking, stops abruptly and talks to another person Distraction – briefly disengaging from a task Organizational Design – the physical layout of the workspace that causes a disruption in workflow Artifacts Not Available – supplies and equipment that are not available in the workspace causing a disruption in workflow Initiator – a person who initiates an interruption Interruption by Organizational Design and Artifacts Not Available were identified as two new categories of interruption. These categories had not previously been cited in the literature. Analysis of the observations indicated that physicians were found to perform slightly fewer activities per hour when compared to RNs. This variance may be attributed to differing roles and responsibilities. Physicians were found to have more activities interrupted when compared to RNs. However, RNs experienced more interruptions per hour. Other people were determined to be the most commonly used medium through which to deliver an interruption. Additional mediums used to deliver an interruption vii included the telephone, pager, and one’s self. Both physicians and RNs were observed to resume an original interrupted activity more often than not. In most interruptions, both physicians and RNs performed only one or two interrupting activities before returning to the original interrupted activity. In conclusion the model was found to explain all interruptions observed during the study. However, the model will require an even more comprehensive study in order to establish its predictive value.

NOVEL INDUCERS OF GLIOTOXIN PRODUCTION IN ASPERGILLUS FUMIGATUS

Secondary metabolites are produced by numerous organisms and can either be benign to humans or harmful. Genes involved in the synthesis and transport of these secondary metabolites are frequently found in gene clusters, which are often located in subtelomeric regions of the chromosome. These clusters are often coordinately regulated, being almost exclusively dependent on transcription factors that are located within the clusters themselves. Secondary metabolites are also regulated by a variety of factors, including nutritional factors, environmental factors and developmental processes. Gliotoxin, which is produced by a variety of Aspergillus species, Trichoderma species, and Penicillium species, exhibits immunosuppressive properties and has therefore been the subject of research for many laboratories. There have been a few proteins shown to regulate the gliotoxin cluster, most notably GliZ, a Zn2Cys6 binuclear finger transcription factor that lies within the cluster, and LaeA, a putative methyltransferase that globally regulates secondary metabolism clusters within numerous fungal organisms, although no study has demonstrated the direct binding of any protein to a promoter region in the gliotoxin cluster. I report here two novel proteins, GipA, a C2H2 transcription factor and GipB, a hybrid sensor kinase, which are involved in regulating the gliotoxin biosynthetic cluster. GipA plays an important role in gliotoxin production, as high-copy expression of gipA induces gliotoxin biosynthesis and loss of gipA reduces gliotoxin biosynthesis by 50%. GipB is also involved in regulating gliotoxin production, as high-copy expression of gipB induces gliotoxin biosynthesis, but only during certain stages of asexual development. Furthermore, loss of gipB reduces gliotoxin biosynthesis by 10%. Based on data obtained from this project, I propose a model for the regulation of gliA, the efflux pump of the gliotoxin cluster, which involves GipB signaling through both GliZ and GipA. I propose that GliZ and GipA are interdependent, as mutation of the GipA DNA binding site in the gliA promoter negatively affects both GliZ-mediated and GipA-mediated induction of gliA. This is further supported by the fact that GliZ cannot fully induce gliA in the absence of GipA and vice versa. This is the first time that anyone has shown evidence of a protein directly binding to the gliotoxin cluster. Even though biosynthetic clusters are often coordinately regulated, my model raises the possibility that gliA is independently regulated, as the layout of the binding site in the gliA promoter is not present upstream of any other genes in the gliotoxin cluster, except for gliZ.

Paraná y su región : Criterios básicos para proyectar políticas de gestión metropolitana

El propósito del presente trabajo es reconocer, cómo a partir de las tensiones globales y locales, de carácter socio - espacial e imbricadas en el proceso de metropolización que acusa el ámbito expandido de la ciudad de Paraná, es factible identificar y analizar los criterios básicos para proyectar una inédita gestión metropolitana dentro del espacio regional que constituyen tanto el ejido de la capital de la Provincia de Entre Ríos como el de sus tres municipios conurbados. En consecuencia y a tal fin, se intenta aquí establecer los nuevos desafíos incorporados a la gestión urbano-ambiental, tanto provincial como municipal, dentro de los que se platea articular la intervención estatal y la participación comunitaria, en el marco de un proceso de ordenamiento territorial del ámbito metropolizado en esas cuatro jurisdicciones en las que hoy habitan más de trescientos mil comprovincianos. Se trata, por tanto, de un proyecto estratégico que institucionalmente persiga la posibilidad de articular en simultáneo cuatro criterios políticos considerados aquí básicos para la gestión democrática del territorio metropolitano: la gobernabilidad, la competitividad, la sustentabilidad y la inclusión social. Criterios que, dentro de esa inteligencia, guiarán el análisis desarrollado sobre propuestas de actuación, jurídico-normativas y de gestión para abordar la compleja problemática del espacio urbano-regional. Es en tal contexto entonces, y dentro de esos principios primordiales para la intervención urbano-territorial, en el que se estima posible plantear, desde el ámbito institucional del Gobierno de la Provincia y de los gobiernos municipales, un acotado y preciso conjunto de políticas sectoriales básicas de ordenamiento que habiliten al despliegue de estrategias comunes y de consenso social posible, destinadas a transformar las críticas condiciones por las que atraviesa la conurbación en su conjunto, sin por eso vulnerar la autonomía de la jurisdicciones municipales incluidas en el espacio geográfico. Concluyendo, en el presente trabajo se analizan e identifican en forma iniciática, los criterios esenciales para el logro de los objetivos territoriales y las metas institucionales que dentro de la gestión metropolitana, puedan asumir el Estado Provincial y las jurisdicciones municipales, en tanto dispositivos gubernamentales destinados a que legal y orgánicamente, sean orientadas las políticas que tengan el propósito de afrontar tanto los procesos territoriales distorsivos como los déficit, las asimetrías y las carencias socio-ambientales que acusa la población del Gran Paraná

El valor de la lectura o cómo eludir el engaño. La trascendencia del romance de la esposa infiel como una lucha indicial

Mediante el estudio de la tradición americana del romance de La esposa infiel, se resalta la importancia que en dicho romance tiene la lucha, por el desciframiento y el encubrimiento de indicios, librada entre el hombre y la mujer. A partir de tal planteo, se propone que su núcleo de interés radica en la problemática del engaño en general y la necesidad de descubrirlo.

El valor de la lectura o cómo eludir el engaño. La trascendencia del romance de la esposa infiel como una lucha indicial

Mediante el estudio de la tradición americana del romance de La esposa infiel, se resalta la importancia que en dicho romance tiene la lucha, por el desciframiento y el encubrimiento de indicios, librada entre el hombre y la mujer. A partir de tal planteo, se propone que su núcleo de interés radica en la problemática del engaño en general y la necesidad de descubrirlo.

Paraná y su región : Criterios básicos para proyectar políticas de gestión metropolitana

El propósito del presente trabajo es reconocer, cómo a partir de las tensiones globales y locales, de carácter socio - espacial e imbricadas en el proceso de metropolización que acusa el ámbito expandido de la ciudad de Paraná, es factible identificar y analizar los criterios básicos para proyectar una inédita gestión metropolitana dentro del espacio regional que constituyen tanto el ejido de la capital de la Provincia de Entre Ríos como el de sus tres municipios conurbados. En consecuencia y a tal fin, se intenta aquí establecer los nuevos desafíos incorporados a la gestión urbano-ambiental, tanto provincial como municipal, dentro de los que se platea articular la intervención estatal y la participación comunitaria, en el marco de un proceso de ordenamiento territorial del ámbito metropolizado en esas cuatro jurisdicciones en las que hoy habitan más de trescientos mil comprovincianos. Se trata, por tanto, de un proyecto estratégico que institucionalmente persiga la posibilidad de articular en simultáneo cuatro criterios políticos considerados aquí básicos para la gestión democrática del territorio metropolitano: la gobernabilidad, la competitividad, la sustentabilidad y la inclusión social. Criterios que, dentro de esa inteligencia, guiarán el análisis desarrollado sobre propuestas de actuación, jurídico-normativas y de gestión para abordar la compleja problemática del espacio urbano-regional. Es en tal contexto entonces, y dentro de esos principios primordiales para la intervención urbano-territorial, en el que se estima posible plantear, desde el ámbito institucional del Gobierno de la Provincia y de los gobiernos municipales, un acotado y preciso conjunto de políticas sectoriales básicas de ordenamiento que habiliten al despliegue de estrategias comunes y de consenso social posible, destinadas a transformar las críticas condiciones por las que atraviesa la conurbación en su conjunto, sin por eso vulnerar la autonomía de la jurisdicciones municipales incluidas en el espacio geográfico. Concluyendo, en el presente trabajo se analizan e identifican en forma iniciática, los criterios esenciales para el logro de los objetivos territoriales y las metas institucionales que dentro de la gestión metropolitana, puedan asumir el Estado Provincial y las jurisdicciones municipales, en tanto dispositivos gubernamentales destinados a que legal y orgánicamente, sean orientadas las políticas que tengan el propósito de afrontar tanto los procesos territoriales distorsivos como los déficit, las asimetrías y las carencias socio-ambientales que acusa la población del Gran Paraná

Paraná y su región : Criterios básicos para proyectar políticas de gestión metropolitana

El propósito del presente trabajo es reconocer, cómo a partir de las tensiones globales y locales, de carácter socio - espacial e imbricadas en el proceso de metropolización que acusa el ámbito expandido de la ciudad de Paraná, es factible identificar y analizar los criterios básicos para proyectar una inédita gestión metropolitana dentro del espacio regional que constituyen tanto el ejido de la capital de la Provincia de Entre Ríos como el de sus tres municipios conurbados. En consecuencia y a tal fin, se intenta aquí establecer los nuevos desafíos incorporados a la gestión urbano-ambiental, tanto provincial como municipal, dentro de los que se platea articular la intervención estatal y la participación comunitaria, en el marco de un proceso de ordenamiento territorial del ámbito metropolizado en esas cuatro jurisdicciones en las que hoy habitan más de trescientos mil comprovincianos. Se trata, por tanto, de un proyecto estratégico que institucionalmente persiga la posibilidad de articular en simultáneo cuatro criterios políticos considerados aquí básicos para la gestión democrática del territorio metropolitano: la gobernabilidad, la competitividad, la sustentabilidad y la inclusión social. Criterios que, dentro de esa inteligencia, guiarán el análisis desarrollado sobre propuestas de actuación, jurídico-normativas y de gestión para abordar la compleja problemática del espacio urbano-regional. Es en tal contexto entonces, y dentro de esos principios primordiales para la intervención urbano-territorial, en el que se estima posible plantear, desde el ámbito institucional del Gobierno de la Provincia y de los gobiernos municipales, un acotado y preciso conjunto de políticas sectoriales básicas de ordenamiento que habiliten al despliegue de estrategias comunes y de consenso social posible, destinadas a transformar las críticas condiciones por las que atraviesa la conurbación en su conjunto, sin por eso vulnerar la autonomía de la jurisdicciones municipales incluidas en el espacio geográfico. Concluyendo, en el presente trabajo se analizan e identifican en forma iniciática, los criterios esenciales para el logro de los objetivos territoriales y las metas institucionales que dentro de la gestión metropolitana, puedan asumir el Estado Provincial y las jurisdicciones municipales, en tanto dispositivos gubernamentales destinados a que legal y orgánicamente, sean orientadas las políticas que tengan el propósito de afrontar tanto los procesos territoriales distorsivos como los déficit, las asimetrías y las carencias socio-ambientales que acusa la población del Gran Paraná

El valor de la lectura o cómo eludir el engaño. La trascendencia del romance de la esposa infiel como una lucha indicial

Mediante el estudio de la tradición americana del romance de La esposa infiel, se resalta la importancia que en dicho romance tiene la lucha, por el desciframiento y el encubrimiento de indicios, librada entre el hombre y la mujer. A partir de tal planteo, se propone que su núcleo de interés radica en la problemática del engaño en general y la necesidad de descubrirlo.