Aplicação do processo de relação interpessoal de Travelbee com mãe de recém-nascido internado em uma unidade neonatal

Objetivou-se aplicar o processo de relação interpessoal com mãe a de um recém-nascido que se encontrava internado em unidade neonatal, utilizando a Teoria da Relação Interpessoal de Joyce Travelbee. Estudo de caso, de natureza descritiva, desenvolvido em unidade de internação neonatal, em dezembro e janeiro de 2004. A Coleta de dados realizou-se mediante entrevista semi-estruturada, com interação pessoa-pessoa. Aplicou-se um instrumento contendo seis perguntas que revelaram possibilidades de ajuda mútua ante os problemas de saúde do filho. Buscou-se amenizar as angústias, dúvidas e medos, por meio da interação com a mãe. Constatou-se que a abordagem teórica foi relevante e serviu como guia para a enfermeira aplicar um cuidar mais ético e humano.

RecA-DNA helical filaments in genetic recombination.

Paul Howard-Flanders et al proposed a molecular model of RecA-mediated recombination reaction six years ago. How does this model stand at present? In answering this question, we focus on two leading ideas of the original model, namely the proposal of the coaxial arrangement of the aligned DNA molecules within helical RecA filaments and the proposal of the ATP independence of the pairing stage of the recombination reaction. Results obtained after the model was proposed are reviewed and compared with these original assumptions and postulates of the model. EM visualization of recombining DNA molecules, studies of the energetics of the RecA-mediated recombination reaction and biochemical analysis of deproteinized joint molecules are fully consistent with a triple-stranded DNA arrangement during the RecA-mediated recombination reaction and demonstrate the ATP independence of the pairing stage of the reaction.

A WiMAX-based implementation of network MIMO for indoor wireless systems

It is well known that multiple-input multiple-output (MIMO) techniques can bring numerous benefits, such as higher spectral efficiency, to point-to-point wireless links. More recently, there has been interest in extending MIMO concepts tomultiuser wireless systems. Our focus in this paper is on network MIMO, a family of techniques whereby each end user in a wireless access network is served through several access points within its range of influence. By tightly coordinating the transmission and reception of signals at multiple access points, network MIMO can transcend the limits on spectral efficiency imposed by cochannel interference. Taking prior information-theoretic analyses of networkMIMO to the next level, we quantify the spectral efficiency gains obtainable under realistic propagation and operational conditions in a typical indoor deployment. Our study relies on detailed simulations and, for specificity, is conducted largely within the physical-layer framework of the IEEE 802.16e Mobile WiMAX system. Furthermore,to facilitate the coordination between access points, we assume that a high-capacity local area network, such as Gigabit Ethernet,connects all the access points. Our results confirm that network MIMO stands to provide a multiple-fold increase in spectralefficiency under these conditions.

Análisis comparativo del monólogo interior de dos fragmentos modernistas

Este trabajo consiste en un análisis comparativo del monólogo interior de dos fragmentos modernistas pertenecientes a The Waves de Virginia Woolf y Ulysses de James Joyce. Asimismo, se contextualizan las dos obras y se definen conceptos relacionados con el monólogo interior, como, el stream of consciousness o el soliloquio.

Partos domiciliares planejados assistidos por enfermeiras obstétricas: transferências maternas e neonatais

O presente trabalho trata-se de estudo exploratório-descritivo que teve como objetivo descrever a taxa e as causas de transferência intraparto para o hospital de mulheres assistidas no domicílio por enfermeiras obstétricas e os desfechos desses nascimentos. A amostra foi composta por onze mulheres e seus recém-nascidos, de janeiro de 2005 a dezembro de 2009. Os dados foram coleta-dos em prontuários e cadernetas de saúde e analisados por estatística descritiva. A taxa de transferência foi de 11%, a maioria de nulíparas (63,6%), e todas foram transferidas durante o primeiro período clínico do parto. Os motivos mais frequentes de transferência foram parada de dilatação cervical e progressão da apresentação fetal, e desproporção cefalopélvica. Os escores de Apgar no 1º e 5º minutos foram >7 em 81,8% dos casos e não houve internação em unidade de terapia intensiva neonatal. Neste estudo constatou-se que o parto domiciliar planejado assistido por enfermeiras obstétricas, com protocolo assistencial, apresentou bons resultados maternos e neonatais, mesmo quando a transferência para o hospital foi necessária.

Q. Horatius Flaccus, Carmina (1-48v), Epodon liber (48v-58v), Carmen saeculare (58v-59v), De arte poetica epistula ad Pisones (60-67), Epistulae (60-90), Sermones (90-122v), cum glossis.

Ce ms. a été donné en 1734 au chapitre de Notre-Dame de Paris par le chanoine Gagne de Périgny: "Ex dono Domini Gagne de Perigny canonici Parisiensis anno 1734" (f. 1). L'ex-libris "A l'église de Paris" (XIXe s.) figure au f. 1, suivi de la cote M9. Notre-Dame.

Florilegium Gallicum.

Ce ms. contient une anthologie de textes des auteurs classiques latins Prudence, Claudien, Ovide, Tibulle, Horace, Juvénal, Perse, Martial, Pétrone, Virgile, Calpurnius, Térence, Salluste, Boèce, Cicéron, Quintilien, Sénèque, Plaute (Querulus), Macrobe, Aulu Gelle, César, Sidoine, Cassiodore, Suétone, Donat. D'après R.H. Rouse, Vincent de Beauvais l'a probablement consulté à Paris avant 1244 pour rédiger son Speculum majus."

Evaluation of the interactions between multiwalled carbon nanotubes and caco-2 cells

The aim of this study was to determine whether multiwalled carbon nanotubes (MWNCT) are taken up by and are toxic to human intestinal enterocytes using the Caco-2 cell model. Caco-2 cells were exposed to 50 ?g/ml MWCNT (oxidized or pristine) for 24 h, and experiments were repeated in the presence of 2.5 mg/L natural organic matter. Cells displayed many of the properties that characterize enterocytes, such as apical microvilli, basolateral basement membrane, and glycogen. The cell monolayers also displayed tight junctions and electrical resistance. Exposure to pristine and oxidized MWCNT, with or without natural organic matter, did not markedly affect viability, which was assessed by measuring activity of released lactate dehydrogenase (LDH) and staining with propidium iodide. Ultrastructural analysis revealed some damage to microvilli colocalized with the MWCNT; however, neither type of MWCNT was taken up by Caco-2 cells. In contrast, pristine and oxidized MWCNT were taken up by the macrophage RAW 264.7 line. Our study suggests that intestinal enterocytes cells do not take up MWCNT. [Authors]

Genome-wide association study for coronary artery calcification with follow-up in myocardial infarction.

BACKGROUND: Coronary artery calcification (CAC) detected by computed tomography is a noninvasive measure of coronary atherosclerosis, which underlies most cases of myocardial infarction (MI). We sought to identify common genetic variants associated with CAC and further investigate their associations with MI. METHODS AND RESULTS: Computed tomography was used to assess quantity of CAC. A meta-analysis of genome-wide association studies for CAC was performed in 9961 men and women from 5 independent community-based cohorts, with replication in 3 additional independent cohorts (n=6032). We examined the top single-nucleotide polymorphisms (SNPs) associated with CAC quantity for association with MI in multiple large genome-wide association studies of MI. Genome-wide significant associations with CAC for SNPs on chromosome 9p21 near CDKN2A and CDKN2B (top SNP: rs1333049; P=7.58×10(-19)) and 6p24 (top SNP: rs9349379, within the PHACTR1 gene; P=2.65×10(-11)) replicated for CAC and for MI. Additionally, there is evidence for concordance of SNP associations with both CAC and MI at a number of other loci, including 3q22 (MRAS gene), 13q34 (COL4A1/COL4A2 genes), and 1p13 (SORT1 gene). CONCLUSIONS: SNPs in the 9p21 and PHACTR1 gene loci were strongly associated with CAC and MI, and there are suggestive associations with both CAC and MI of SNPs in additional loci. Multiple genetic loci are associated with development of both underlying coronary atherosclerosis and clinical events.

Avaliação do projeto Final - Recursos Hídrico do Tarrafal – Cabo Verde

A avaliacão do projecto do recursos hidricos do Tarrafal (Cabo Verde ) tem por base dois objectivos: 1) Cumprir as obrigag8es do contrato, o qual roquor uwa avaliação.o final, a 2) Sujeitar-se aon requisitos da CID pars a avaliação final. Desta formal a extenslo deta avaliaqlo 6 male quo ura simples rovialo, reopondendo a obrigaqos do contracto. Esta avaliagKot tern por fim estimar a forma coo o projecto atinglu ou doixou do atingir os objectivos propostos. An recomendaga4s quo safram desta avalia qio dovem sorvir para melhorar os projectos om andamento quo integram no sistema da CID. Ambos o fundos da CID o do contracto foram utilizados nesta avaliaqlo Os pontos apresentados nests documento slo da equipe do avaliaqlo o n~o representan necessariamente 0s da Administraqlo o/ou do Executivo da CID. B. A.VLICrUi)Z DA Ai1ALIAj!O Todo o projecto do Recurnos Hfdricos do Tarrafal eat inserido dontro do pris1m desta avalia.lo. Zbora outrou relatdrios tenham servido do fontes do infor=qlo, a proeantj oxamina o projecto dead. o porfodo da preparaqEo at o trmino C. EQU!!' DE AYALIACKO A equioe roi Joloeada polo Dean ( tipo do reitor do faculdads )# Director da Cemara don Adminintradoras da CID Dr. drnont Briskoyp o polo Director do Executivo, Dr. John L. Fiecher. A avaliaqgo foi orientada pole repartiglo do Executivo da CID. 0 Dr. Barry Re. Baintonp agents director do projectop coordenou o processo do avaliaqEo. 0 Dr. Gerald 3stlockq director do programus Internacionais do agricultura na Universidade do Arizonap foi selocionado coo lider da equips . 0 Dr. Howard Peterson, professor do agriculture • ongenharia do irrgaqIo da Universidade do Utah, foi selecionado coma segundo mumbro da equips. 0 Dr. Peterson ji tinha cumprido dues misses no projecto em Cabo Verde. 0 Dr. Jean Ruley Kearns, conselheiro director da CID no poriodo do 1982-83p fot selecionado comc sondo o tarceiro membro da equipe. D. ATODO DE AVALIAQXO Os pianos proliminares para conduzir a avaliaqo foram feitos numa rounibo inicial em Tucson ( Arizona ) a 5 do novembro do 1982. Nessa rounilo foi deci dido quo o Dr. Matlock visitaria o projecto durante o afs do novembro do 1982. A visita fot planojada do forma a coincidir com a presenga do Kern Stutlor, director coordenador do projectop quo devoria astar am Cabo lirde nessa data co.mpletando os trabalho a preparando o relat6rio final. Uma c6pia do Procasio do Avaliaglo pola AID assim como cdpias do relatdrios anteriores a outros docunontos rolevantea foram diatribufdos aos membros da equips duranto a reunigo inicial. Oa docu ientos examinados polos membros da equip. encontram-se listados na secção /III, Referiencias

Death and resurrection of the human IRGM gene

Immunity-related GTPases (IRG) play an important role in defense against intracellular pathogens. One member of this gene family in humans, IRGM, has been recently implicated as a risk factor for Crohn's disease. We analyzed the detailed structure of this gene family among primates and showed that most of the IRG gene cluster was deleted early in primate evolution, after the divergence of the anthropoids from prosimians ( about 50 million years ago). Comparative sequence analysis of New World and Old World monkey species shows that the single-copy IRGM gene became pseudogenized as a result of an Alu retrotransposition event in the anthropoid common ancestor that disrupted the open reading frame (ORF). We find that the ORF was reestablished as a part of a polymorphic stop codon in the common ancestor of humans and great apes. Expression analysis suggests that this change occurred in conjunction with the insertion of an endogenous retrovirus, which altered the transcription initiation, splicing, and expression profile of IRGM. These data argue that the gene became pseudogenized and was then resurrected through a series of complex structural events and suggest remarkable functional plasticity where alleles experience diverse evolutionary pressures over time. Such dynamism in structure and evolution may be critical for a gene family locked in an arms race with an ever-changing repertoire of intracellular parasites.

Crystal packing modifies ligand binding affinity: the case of aldose reductase.

The relationship between the structures of protein-ligand complexes existing in the crystal and in solution, essential in the case of fragment-based screening by X-ray crystallography (FBS-X), has been often an object of controversy. To address this question, simultaneous co-crystallization and soaking of two inhibitors with different ratios, Fidarestat (FID; K(d) = 6.5 nM) and IDD594 (594; K(d) = 61 nM), which bind to h-aldose reductase (AR), have been performed. The subatomic resolution of the crystal structures allows the differentiation of both inhibitors, even when the structures are almost superposed. We have determined the occupation ratio in solution by mass spectrometry (MS) Occ(FID)/Occ(594) = 2.7 and by X-ray crystallography Occ(FID)/Occ(594) = 0.6. The occupancies in the crystal and in solution differ 4.6 times, implying that ligand binding potency is influenced by crystal contacts. A structural analysis shows that the Loop A (residues 122-130), which is exposed to the solvent, is flexible in solution, and is involved in packing contacts within the crystal. Furthermore, inhibitor 594 contacts the base of Loop A, stabilizing it, while inhibitor FID does not. This is shown by the difference in B-factors of the Loop A between the AR-594 and AR-FID complexes. A stable loop diminishes the entropic energy barrier to binding, favoring 594 versus FID. Therefore, the effect of the crystal environment should be taken into consideration in the X-ray diffraction analysis of ligand binding to proteins. This conclusion highlights the need for additional methodologies in the case of FBS-X to validate this powerful screening technique, which is widely used.

Estudio de la edad, el crecimiento, la madurez sexual y el desove de la anchoveta (Cetengraulis Mysticetus) en el Golfo de Panamá

Realiza un estudio sobre la edad, crecimiento, madurez sexual y las épocas de desove de la anchoveta. Estas investigaciones se realizaron en el Golfo de Panamá entre los años de junio de 1951 a enero de 1956.

Estudio de la edad, el crecimiento, la madurez sexual y el desove de la anchoveta (Cetengraulis Mysticetus) en el Golfo de Panamá (Conclusión)

Presenta los métodos utilizados en los laboratorios para determinar la madurez sexual y desove de la población de anchovetas, su época y frecuencia. Estos datos concluyen la edad con relación al tiempo del desove anual gracias al índice de gónadas recolectado en el Golfo de Panamá.

Genome-wide association analysis of blood-pressure traits in African-ancestry individuals reveals common associated genes in African and non-African populations.

High blood pressure (BP) is more prevalent and contributes to more severe manifestations of cardiovascular disease (CVD) in African Americans than in any other United States ethnic group. Several small African-ancestry (AA) BP genome-wide association studies (GWASs) have been published, but their findings have failed to replicate to date. We report on a large AA BP GWAS meta-analysis that includes 29,378 individuals from 19 discovery cohorts and subsequent replication in additional samples of AA (n = 10,386), European ancestry (EA) (n = 69,395), and East Asian ancestry (n = 19,601). Five loci (EVX1-HOXA, ULK4, RSPO3, PLEKHG1, and SOX6) reached genome-wide significance (p < 1.0 × 10(-8)) for either systolic or diastolic BP in a transethnic meta-analysis after correction for multiple testing. Three of these BP loci (EVX1-HOXA, RSPO3, and PLEKHG1) lack previous associations with BP. We also identified one independent signal in a known BP locus (SOX6) and provide evidence for fine mapping in four additional validated BP loci. We also demonstrate that validated EA BP GWAS loci, considered jointly, show significant effects in AA samples. Consequently, these findings suggest that BP loci might have universal effects across studied populations, demonstrating that multiethnic samples are an essential component in identifying, fine mapping, and understanding their trait variability.