Mechanisms governing lentivirus integration site selection.

An essential step of the life cycle of retroviruses is the stable insertion of a copy of their DNA genome into the host cell genome, and lentiviruses are no exception. This integration step, catalyzed by the viral-encoded integrase, ensures long-term expression of the viral genes, thus allowing a productive viral replication and rendering retroviral vectors also attractive for the field of gene therapy. At the same time, this ability to integrate into the host genome raises safety concerns regarding the use of retroviral-based gene therapy vectors, due to the genomic locations of integration sites. The availability of the human genome sequence made possible the analysis of the integration site preferences, which revealed to be nonrandom and retrovirus-specific, i.e. all lentiviruses studied so far favor integration in active transcription units, while other retroviruses have a different integration site distribution. Several mechanisms have been proposed that may influence integration targeting, which include (i) chromatin accessibility, (ii) cell cycle effects, and (iii) tethering proteins. Recent data provide evidence that integration site selection can occur via a tethering mechanism, through the recruitment of the lentiviral integrase by the cellular LEDGF/p75 protein, both proteins being the two major players in lentiviral integration targeting.

Generació automàtica d'una solució JEE (JPA + EJB + JSF) basada en el framework AndroMDA

En aquest projecte s'utilitzarà el framework AndroMDA per construir un programari que permeti generar automàticament a partir d'un model UML simple una aplicació JEE.

Regional-scale integration of multi-scale hydrological and geophysical data using a two-step Bayesian sequential simulation approach

Significant progress has been made with regard to the quantitative integration of geophysical and hydrological data at the local scale for the purpose of improving predictions of groundwater flow and solute transport. However, extending corresponding approaches to the regional scale still represents one of the major challenges in the domain of hydrogeophysics. To address this problem, we have developed a regional-scale data integration methodology based on a two-step Bayesian sequential simulation approach. Our objective is to generate high-resolution stochastic realizations of the regional-scale hydraulic conductivity field in the common case where there exist spatially exhaustive but poorly resolved measurements of a related geophysical parameter, as well as highly resolved but spatially sparse collocated measurements of this geophysical parameter and the hydraulic conductivity. To integrate this multi-scale, multi-parameter database, we first link the low- and high-resolution geophysical data via a stochastic downscaling procedure. This is followed by relating the downscaled geophysical data to the high-resolution hydraulic conductivity distribution. After outlining the general methodology of the approach, we demonstrate its application to a realistic synthetic example where we consider as data high-resolution measurements of the hydraulic and electrical conductivities at a small number of borehole locations, as well as spatially exhaustive, low-resolution estimates of the electrical conductivity obtained from surface-based electrical resistivity tomography. The different stochastic realizations of the hydraulic conductivity field obtained using our procedure are validated by comparing their solute transport behaviour with that of the underlying ?true? hydraulic conductivity field. We find that, even in the presence of strong subsurface heterogeneity, our proposed procedure allows for the generation of faithful representations of the regional-scale hydraulic conductivity structure and reliable predictions of solute transport over long, regional-scale distances.

Framework para la capa de presentación de aplicaciones web

El framework dissenyat, denominat magis, ajuda als desenvolupadors organitzant la presentació de continguts, l'accés a les funcions i serveis de negoci, el flux de pàgines, el processament de formularis, el maneig d'errors i la gestió d'estat.

J2EE Framework capa de presentación

El present projecte se centra en la creació d'un framework1 per a la capa de presentació de java J2EE2, així com un generador de codi de suport a aquest.

Diseño e implementación de un marco de trabajo(framework) de presentación para aplicaciones J2EE

El projecte de fi de carrera proposat consisteix en l'anàlisi, disseny i implementació d'un conjunt de components que simplifiquen la construcció de la capa de presentació per a aplicacions web, servint de base per al desenvolupament d'altres productes.

A framework for quality in long-term residential care for older people in Ireland / National Council on Ageing and Older People

In 1999 the National Council on Ageing and Older People commissioned a postal survey of all long-term residential care facilities in the country to determie: - whether facilities had quality initiatives in operation - providers' views and aspirations for future provision of long-term care - providers' views on the introduction of a national quality monitoring policy This report is the outcome of the programme of work conducted by the Council on the quality of long-term residential care provision for older people in Ireland. The aim of the report is to provide a framework for developing quality in long-term residential care settings with a focus on the well-being, dignity and autonomy of older residents.

Palliative Care - A Five Year/Medium Term Development Framework

This document details the HSE's Palliative Care Services - Five Year Medium Term Development Framework (2009 - 2013). It has been developed using a holistic, system-wide, approach to addressing the level of need identified by both the Baseline Study on the Provision of Hospice / Specialist Palliative Care Services in Ireland (2006) as well as the HSE Audit of Palliative Care Service Provision (2007). The HSE Audit was conducted by each of the four HSE Administrative Areas, in conjunction with Area Development Committees, against the recommendations of the Report of the National Advisory Committee on Palliative Care 2001 (the NACPC Report). Therefore, this document is grounded in the recommendations of the NACPC Report, (e.g. palliative care definition, palliative care service areas, staffing and bed number ratios), and informed by the findings of the HSE Audit. The purpose of this document is not to replace the recommendations of the NACPC Report from a policy context, nor does it represent a new national strategy for palliative care. This document details the required actions and initiatives necessary to address the gaps in palliative care service provision, against the recommendations set out in the NACPC Report.

Active East - A Physical Activity Framework for the East of England 2006

This document sets out the role of physical activity, not just in terms of its known health benefits (prevention of obesity), but also its wider social contribution. Physical activity is a cross cutting agenda and needs to involve a variety of organisations working in partnership with the health sector.

Leaner East: an obesity framework for the East of England 2006-2010

This framework outlines how the East of England will tackle the rise in the proportion of the population who are overweight and provide treatment for obesity.

J2EE. Diseño e implementación de un Framework de Persistencia

Anàlisi i desenvolupament d'un conjunt de components que simplifiquin i agilitin el desenvolupament de la capa de persistència en aplicacions multicapa, especialment per a les aplicacions desenvolupades amb J2EE.

Implementació d'una funcionalitat en un framework per una aplicació J2EE

En el present projecte utilitzarem els coneixements sobre els frameworks adquirits al llarg del treball per complir la raó última d'aquest projecte: la modificació d'un framework per tal d'ampliar o millorar el seu rendiment en l'aplicació J2EE. En concret consistirà en la implementació d¿un validador en el framework JSF.

The Coronary Heart Disease National Service Framework: Building on excellence,maintaining progress - Progress report for 2008

This report details the progress which has been made in implementing the Coronary Heart Disease National Service Framework in the eight years since its publication.

Desenvolupament amb tecnologia Microsoft.net Framework arquitectura Asp.net : Aplicació de comerç electrònic

Tot i que el cos d'aquest treball es refereix a la creació d'una botiga virtual per a la venda de bicicletes i altres components que s'hi relacionen, l'objectiu últim és conèixer l'arquitectura Asp.NET ?integrada dins la tecnologia Microsoft.NET Framework? i aprofundir en les seves característiques.

Standard Evaluation Framework for Weight Management Interventions - Core Criteria

The National Obesity Observatory was established to provide a single point of contact for wide-ranging authoritative information on data and evidence related to obesity, overweight, underweight and their determinants. The Standard Evaluation Framework is a list of data collection criteria and supporting guidance for collecting high quality information to support the evaluation of weight management interventions. This is a quick reference guide to the core criteria of the Standard Evaluation Framework. Essential criteria are presented as the minimum recommended data for evaluating a weight management intervention. Desirable criteria are additional data that would enhance the evaluation.refer to the resource